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The map label for this gene is rpoN [H]

Identifier: 183221037

GI number: 183221037

Start: 1725094

End: 1726515

Strand: Direct

Name: rpoN [H]

Synonym: LEPBI_I1650

Alternate gene names: 183221037

Gene position: 1725094-1726515 (Clockwise)

Preceding gene: 183221036

Following gene: 183221038

Centisome position: 47.92

GC content: 37.62

Gene sequence:

>1422_bases
ATGAAACTCGGGGCTTCACTTTCACACCGCCAAACACAGAAACTGGTGATGACCCAGGACTTACGTCAGTCCATAGAACT
TTTATCCTTATCCACATTAGAACTTTCTGATAAAATCCAAAACGAATTACTTGAGAATCCTCTTCTCGATGAATTAGGCG
TTGATGAAAAAACAAAAATGCCAGAACTATTTTCCATCGATGAAGTGAAACGATTGGAAAAATTAAATCATGAAAAGAGT
ACAGATGTCAATTGGCAAGACAGTTATTCTTTGGAAGGTCCAAGGACTTATGATTCTGAAGCGAGTGACAGAAACCAAAA
ATATATCGAATCCTCGACAAGAGGTGAAACCTTAGAGGAACACCTGTTAAATCAACTTCGTCTTATCAAACTCACAAAAT
TAGAGTTTGAAATTGGGGAAGTTCTCATCAGTATGATCGATGATAAAGGATTCATCACTGATGATTTGACATTGGTAGCC
AAAGAGATGGGTTATCCGGAACCAAAACTTCGTCGTGTGTTACAAGTCATCAATGAGCTTGACCCGATTGGCATTGGTGC
AAAGGACATGCAAGAAACCCTTCTCATCCAAGGGAGGATTTTATTTCCAGAAAACACTCTTTTACACCAATTAATTGGCG
AGTTTTTATCTGATTTAGAAAAGGTAGATTATAAAAAAATCGCCAAGAATTTAAAAATCACAGAAGAAGAAATTTTAAGT
TTAGCAAGGTTAATTAAAAAGTTAGAACCATATCCTGCGACCACCTACCAAGGACGAAAAATCGATTATGTGGTAGCGGA
TGTAGTGGTCAAAGAAGTGGGAAATGAATTTAACATTTTTATCAATGATGAATGGTTACCAAAACTAACAATCCAAGAAG
AATACAAAGAACTCCTCGGACAAAAACTCCCACCGAAAGAAAAGGAATACTTCCAGACAAAATATAGTTCGGCGCAGTGG
CTCATTCGTTCGATCCAACAAAGGAGACAAACCTTACAAAGAGTTGTGAGTTGTATCATTGATTTCCAGGTGGATTTTTT
TCGCGGAGGGATTGGATTTATCAAACCCCTGACTCTGAAAGAAGTCGCTGAAAAACTAGGCCTTCACGAATCGACAATTT
CAAGAATTACAACGAATAAATACATCCAAACCACATGGGGTATTTTTGAATTAAAATGGTTTTTTTCATCAGGTGTGAAA
TCCGCAGAAGGTGGAAAAGAAAGTTCGAAAAAAATCCATGAGATCATTCGGAATTTGGTGAAAGAAGAAGATGAACAAAA
TCCTTTGTCCGACCAAGACATCGTCGAACTGATGGAAAAAAAAGGTATTGAAATTGCTCGTAGAACAGTGGCAAAATACC
GAAAGGTTTTAAGAATCCTTCCTTCCAACGAAAGAAAGCGAATCAGTTCGCTCAAGGGGTAA

Upstream 100 bases:

>100_bases
CATGTATAGTGGTCGAATATTAATTTCTGGAACTGCTGATGACTTAATCAATGATCCCGAAACACGTAGAATTTATTTAG
GTGAGGATTTTAAACTATAA

Downstream 100 bases:

>100_bases
ACAATGCCAGTTCCTGGAATCACAGTGGAAACCATTCTCCGAGACCACGACGACTTACAATTGCAACTCGTGACTGGCGA
AGTTGGTCTTTCGAATCGAA

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKLGASLSHRQTQKLVMTQDLRQSIELLSLSTLELSDKIQNELLENPLLDELGVDEKTKMPELFSIDEVKRLEKLNHEKS
TDVNWQDSYSLEGPRTYDSEASDRNQKYIESSTRGETLEEHLLNQLRLIKLTKLEFEIGEVLISMIDDKGFITDDLTLVA
KEMGYPEPKLRRVLQVINELDPIGIGAKDMQETLLIQGRILFPENTLLHQLIGEFLSDLEKVDYKKIAKNLKITEEEILS
LARLIKKLEPYPATTYQGRKIDYVVADVVVKEVGNEFNIFINDEWLPKLTIQEEYKELLGQKLPPKEKEYFQTKYSSAQW
LIRSIQQRRQTLQRVVSCIIDFQVDFFRGGIGFIKPLTLKEVAEKLGLHESTISRITTNKYIQTTWGIFELKWFFSSGVK
SAEGGKESSKKIHEIIRNLVKEEDEQNPLSDQDIVELMEKKGIEIARRTVAKYRKVLRILPSNERKRISSLKG

Sequences:

>Translated_473_residues
MKLGASLSHRQTQKLVMTQDLRQSIELLSLSTLELSDKIQNELLENPLLDELGVDEKTKMPELFSIDEVKRLEKLNHEKS
TDVNWQDSYSLEGPRTYDSEASDRNQKYIESSTRGETLEEHLLNQLRLIKLTKLEFEIGEVLISMIDDKGFITDDLTLVA
KEMGYPEPKLRRVLQVINELDPIGIGAKDMQETLLIQGRILFPENTLLHQLIGEFLSDLEKVDYKKIAKNLKITEEEILS
LARLIKKLEPYPATTYQGRKIDYVVADVVVKEVGNEFNIFINDEWLPKLTIQEEYKELLGQKLPPKEKEYFQTKYSSAQW
LIRSIQQRRQTLQRVVSCIIDFQVDFFRGGIGFIKPLTLKEVAEKLGLHESTISRITTNKYIQTTWGIFELKWFFSSGVK
SAEGGKESSKKIHEIIRNLVKEEDEQNPLSDQDIVELMEKKGIEIARRTVAKYRKVLRILPSNERKRISSLKG
>Mature_473_residues
MKLGASLSHRQTQKLVMTQDLRQSIELLSLSTLELSDKIQNELLENPLLDELGVDEKTKMPELFSIDEVKRLEKLNHEKS
TDVNWQDSYSLEGPRTYDSEASDRNQKYIESSTRGETLEEHLLNQLRLIKLTKLEFEIGEVLISMIDDKGFITDDLTLVA
KEMGYPEPKLRRVLQVINELDPIGIGAKDMQETLLIQGRILFPENTLLHQLIGEFLSDLEKVDYKKIAKNLKITEEEILS
LARLIKKLEPYPATTYQGRKIDYVVADVVVKEVGNEFNIFINDEWLPKLTIQEEYKELLGQKLPPKEKEYFQTKYSSAQW
LIRSIQQRRQTLQRVVSCIIDFQVDFFRGGIGFIKPLTLKEVAEKLGLHESTISRITTNKYIQTTWGIFELKWFFSSGVK
SAEGGKESSKKIHEIIRNLVKEEDEQNPLSDQDIVELMEKKGIEIARRTVAKYRKVLRILPSNERKRISSLKG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=479, Percent_Identity=32.776617954071, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54744; Mature: 54744

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGASLSHRQTQKLVMTQDLRQSIELLSLSTLELSDKIQNELLENPLLDELGVDEKTKM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCC
PELFSIDEVKRLEKLNHEKSTDVNWQDSYSLEGPRTYDSEASDRNQKYIESSTRGETLEE
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
HLLNQLRLIKLTKLEFEIGEVLISMIDDKGFITDDLTLVAKEMGYPEPKLRRVLQVINEL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCC
DPIGIGAKDMQETLLIQGRILFPENTLLHQLIGEFLSDLEKVDYKKIAKNLKITEEEILS
CCCCCCHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LARLIKKLEPYPATTYQGRKIDYVVADVVVKEVGNEFNIFINDEWLPKLTIQEEYKELLG
HHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHC
QKLPPKEKEYFQTKYSSAQWLIRSIQQRRQTLQRVVSCIIDFQVDFFRGGIGFIKPLTLK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHH
EVAEKLGLHESTISRITTNKYIQTTWGIFELKWFFSSGVKSAEGGKESSKKIHEIIRNLV
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
KEEDEQNPLSDQDIVELMEKKGIEIARRTVAKYRKVLRILPSNERKRISSLKG
HCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure
MKLGASLSHRQTQKLVMTQDLRQSIELLSLSTLELSDKIQNELLENPLLDELGVDEKTKM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCC
PELFSIDEVKRLEKLNHEKSTDVNWQDSYSLEGPRTYDSEASDRNQKYIESSTRGETLEE
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
HLLNQLRLIKLTKLEFEIGEVLISMIDDKGFITDDLTLVAKEMGYPEPKLRRVLQVINEL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCC
DPIGIGAKDMQETLLIQGRILFPENTLLHQLIGEFLSDLEKVDYKKIAKNLKITEEEILS
CCCCCCHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LARLIKKLEPYPATTYQGRKIDYVVADVVVKEVGNEFNIFINDEWLPKLTIQEEYKELLG
HHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHC
QKLPPKEKEYFQTKYSSAQWLIRSIQQRRQTLQRVVSCIIDFQVDFFRGGIGFIKPLTLK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHH
EVAEKLGLHESTISRITTNKYIQTTWGIFELKWFFSSGVKSAEGGKESSKKIHEIIRNLV
HHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
KEEDEQNPLSDQDIVELMEKKGIEIARRTVAKYRKVLRILPSNERKRISSLKG
HCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113171; 10984043 [H]