Click here to switch to the map view.

The map label for this gene is cysM [H]

Identifier: 183221015

GI number: 183221015

Start: 1700819

End: 1701820

Strand: Direct

Name: cysM [H]

Synonym: LEPBI_I1628

Alternate gene names: 183221015

Gene position: 1700819-1701820 (Clockwise)

Preceding gene: 183221014

Following gene: 183221017

Centisome position: 47.25

GC content: 42.32

Gene sequence:

>1002_bases
ATGATTGATCCGATTTCCAAAGGTATCGATGACTTTGGCAACAGTTTGTTGCAAGCACTGAATAATGTACAGGGTATCTT
TGGAAAGGAACTTTCCGTTGCCAAACCAATCAAAGACAATGTTTTACAATTGATTGGCAATACACCTCTCATTCGTTTGA
ACCGGATTGGATCTGAATATTCTGGTGTACAGTTTTACCTAAAAGCTGAATTTTTAAATCCAACAGGTTCTGCGAAAGAT
CGTACTGCCATAGCGATGGTGTTAGATGCGGAAAAACGAGGGAAGTTAAAAAAAGGAATGCCTATCATTCTTTCTGGTGC
CGGTTCCTCCTCTGTGAGTTTTACATGGATTGGAAAGGTCAAAGGTTACCCTGTGTATTGCCTAGTTCCCCTCACCACAC
CACCAGAACGTGTCCAACTCCTCAGAAGTTATGGAGCAGAAGTGACGGTTACGAATGAATCGGATATCGTAAAGCTCACG
GAACTAGCAGAAGAAAAAGCCAAAAAAATGGGTGGGTATCTCCCTGATGAATTGGAAAATCCTGCCAACCCCAATTTCCA
TTTCAAAACCACAGGTCCTGAAATATGGAGAGATCTGCAAGGAAAAGTCGGAGCTGTGATTTCAGCACCAGGATCTGGTG
GTGCCATTACTGGGATCGGTCGTTACCTGAAGTCACAAGACAGAAGGGTAAAAGTGATCATTGCAGGAAAACAAAACTCA
GCCTTTATGGAATATGGAAAAACTGACAATCCCAAAGAAAGAGAAAGGATTCGCCTGCCTGCCGTTTATGATCCAAAACT
CATTGACCATTACTTTCATGTCACAAAAGACGAAGCCTTACACCTGCAAGCCGATCTATATGAAAAAGAAGGTATTTTTG
CTGGGCTTACAACTGGGACAGTGATCACAGGTGCTTTACGTTTTTCGGAAACTTTATCTGAGTCCGAAAAAAATGAAAGG
AACCCGTTTAATATCGTGATCCTCTCCCCCGACAGAGATTAA

Upstream 100 bases:

>100_bases
GACCATTCCTCCCAAATATGGATATGGTGACAGAGCAGTTGGACCCATTCCTGCCAATTCGACTCTTGTGTTTGATGTTG
AGTTAATTAAAGTTAAATAA

Downstream 100 bases:

>100_bases
TTTTTTTCGCTAAACAGTTGTTTGATTTCCTGTAAGGAACTTTTGGCTACAGTTTCGCCGAGTTCAATGAATTCTTTACT
CCGCCAAAACTCAAACCAAT

Product: cysteine synthase B

Products: NA

Alternate protein names: CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MIDPISKGIDDFGNSLLQALNNVQGIFGKELSVAKPIKDNVLQLIGNTPLIRLNRIGSEYSGVQFYLKAEFLNPTGSAKD
RTAIAMVLDAEKRGKLKKGMPIILSGAGSSSVSFTWIGKVKGYPVYCLVPLTTPPERVQLLRSYGAEVTVTNESDIVKLT
ELAEEKAKKMGGYLPDELENPANPNFHFKTTGPEIWRDLQGKVGAVISAPGSGGAITGIGRYLKSQDRRVKVIIAGKQNS
AFMEYGKTDNPKERERIRLPAVYDPKLIDHYFHVTKDEALHLQADLYEKEGIFAGLTTGTVITGALRFSETLSESEKNER
NPFNIVILSPDRD

Sequences:

>Translated_333_residues
MIDPISKGIDDFGNSLLQALNNVQGIFGKELSVAKPIKDNVLQLIGNTPLIRLNRIGSEYSGVQFYLKAEFLNPTGSAKD
RTAIAMVLDAEKRGKLKKGMPIILSGAGSSSVSFTWIGKVKGYPVYCLVPLTTPPERVQLLRSYGAEVTVTNESDIVKLT
ELAEEKAKKMGGYLPDELENPANPNFHFKTTGPEIWRDLQGKVGAVISAPGSGGAITGIGRYLKSQDRRVKVIIAGKQNS
AFMEYGKTDNPKERERIRLPAVYDPKLIDHYFHVTKDEALHLQADLYEKEGIFAGLTTGTVITGALRFSETLSESEKNER
NPFNIVILSPDRD
>Mature_333_residues
MIDPISKGIDDFGNSLLQALNNVQGIFGKELSVAKPIKDNVLQLIGNTPLIRLNRIGSEYSGVQFYLKAEFLNPTGSAKD
RTAIAMVLDAEKRGKLKKGMPIILSGAGSSSVSFTWIGKVKGYPVYCLVPLTTPPERVQLLRSYGAEVTVTNESDIVKLT
ELAEEKAKKMGGYLPDELENPANPNFHFKTTGPEIWRDLQGKVGAVISAPGSGGAITGIGRYLKSQDRRVKVIIAGKQNS
AFMEYGKTDNPKERERIRLPAVYDPKLIDHYFHVTKDEALHLQADLYEKEGIFAGLTTGTVITGALRFSETLSESEKNER
NPFNIVILSPDRD

Specific function: Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=305, Percent_Identity=29.1803278688525, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI295821200, Length=305, Percent_Identity=29.1803278688525, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI4557415, Length=305, Percent_Identity=29.1803278688525, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI2367138, Length=269, Percent_Identity=35.6877323420074, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1788754, Length=321, Percent_Identity=33.6448598130841, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17535051, Length=274, Percent_Identity=35.4014598540146, Blast_Score=169, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17562970, Length=273, Percent_Identity=33.6996336996337, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI32566674, Length=273, Percent_Identity=33.6996336996337, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI115535073, Length=279, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17561720, Length=273, Percent_Identity=34.4322344322344, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI32566672, Length=188, Percent_Identity=30.8510638297872, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17534315, Length=314, Percent_Identity=30.5732484076433, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25147552, Length=309, Percent_Identity=29.126213592233, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6321594, Length=314, Percent_Identity=30.5732484076433, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321449, Length=328, Percent_Identity=30.4878048780488, Blast_Score=124, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24643623, Length=308, Percent_Identity=30.5194805194805, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI20129101, Length=308, Percent_Identity=30.5194805194805, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005858
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 36583; Mature: 36583

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPISKGIDDFGNSLLQALNNVQGIFGKELSVAKPIKDNVLQLIGNTPLIRLNRIGSEY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCC
SGVQFYLKAEFLNPTGSAKDRTAIAMVLDAEKRGKLKKGMPIILSGAGSSSVSFTWIGKV
CCEEEEEEEEEECCCCCCCCCEEEEEEEECHHCCCHHCCCCEEEECCCCCCEEEEEEECC
KGYPVYCLVPLTTPPERVQLLRSYGAEVTVTNESDIVKLTELAEEKAKKMGGYLPDELEN
CCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHCC
PANPNFHFKTTGPEIWRDLQGKVGAVISAPGSGGAITGIGRYLKSQDRRVKVIIAGKQNS
CCCCCEEEEECCHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCC
AFMEYGKTDNPKERERIRLPAVYDPKLIDHYFHVTKDEALHLQADLYEKEGIFAGLTTGT
HHHHCCCCCCCCCHHHEECCCCCCCHHHHHHHHCCCCCEEEEEEHHHHCCCEEEEECCCH
VITGALRFSETLSESEKNERNPFNIVILSPDRD
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
>Mature Secondary Structure
MIDPISKGIDDFGNSLLQALNNVQGIFGKELSVAKPIKDNVLQLIGNTPLIRLNRIGSEY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCC
SGVQFYLKAEFLNPTGSAKDRTAIAMVLDAEKRGKLKKGMPIILSGAGSSSVSFTWIGKV
CCEEEEEEEEEECCCCCCCCCEEEEEEEECHHCCCHHCCCCEEEECCCCCCEEEEEEECC
KGYPVYCLVPLTTPPERVQLLRSYGAEVTVTNESDIVKLTELAEEKAKKMGGYLPDELEN
CCCEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHCC
PANPNFHFKTTGPEIWRDLQGKVGAVISAPGSGGAITGIGRYLKSQDRRVKVIIAGKQNS
CCCCCEEEEECCHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCC
AFMEYGKTDNPKERERIRLPAVYDPKLIDHYFHVTKDEALHLQADLYEKEGIFAGLTTGT
HHHHCCCCCCCCCHHHEECCCCCCCHHHHHHHHCCCCCEEEEEEHHHHCCCEEEEECCCH
VITGALRFSETLSESEKNERNPFNIVILSPDRD
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503 [H]