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The map label for this gene is fliM [H]

Identifier: 183220997

GI number: 183220997

Start: 1684640

End: 1685665

Strand: Direct

Name: fliM [H]

Synonym: LEPBI_I1610

Alternate gene names: 183220997

Gene position: 1684640-1685665 (Clockwise)

Preceding gene: 183220996

Following gene: 183220998

Centisome position: 46.8

GC content: 45.32

Gene sequence:

>1026_bases
ATGACGGAAATCCTTTCCCAAGACGAAATTGATGCCCTGTTAAATGCCATCTCCTCTGGAGAAGTTTCGGAGGATGAATA
CTCGTCCGTTGGGGAACAAAAAAAAGTCAAAATTTACGACTTCAAACGTCCGGATAAATTTTCAAAAGACCAAATTCGTA
CACTCCAGATGATGCACGAGATCTTTGCTCGTTTGGCAACCACAGGACTTTCTGCACAGCTCCGAGCCCTCGTCGTAGTG
CACGTAGCTTCGGTGGACCAGTTAACTTACGAAGAATTCATTCGTTCCATTCCAAATCCCACAACCCTTGCTGTGATCAA
TATGGACCCTCTCCGAGGATCCGCCATTTTAGAAATTGACCCTTCCATTTCCTTTACCATCATCGATCGTCTGTTTGGTG
GTAAGGGTGAGTCTTCGAAAGTCAACCGTGAACTTTCCGATATTGAGTTATCGGTAATGGAAGGGATCATCGTCAGAATC
CTTGGAAACTTACGCGAGTCTTGGTCAACGGTGATCGACTTACGTCCTAGGCTCGGAAACATCGAAACAAACCCGCAGTT
CGCACAGGTTGTTCCTCCCAATGACATGGTGGTATTAATTACCTTGGAAACAAAAGTAGGGGAAGTGGAAAGGATGACAA
ACCTTTGTATTCCTTACATCACCATTGAACCTATCATCAATAAACTCTCTGCACAGTATTGGTATTCCTCGATTCGAAAA
GGGGAAGTGGATGAAAACCGTGCCGTCATCCAAGAACGTCTCGACCAAGTGAAAATCCCTCTCATTTCTGAAGTGGGAAG
CGTGGATATTTCTCTCAATGATCTCATGAACTTACATGTCGGAGATGTGATCAAATTGGAGAATACGCCCATCAAAACCG
ACCTGATGGTGAAAGTGGGGGACAGAAGTAAATTCAAAGCCACACCAGGACGCGTGGGAAACCGCCTAGCTATCCAAATT
GGTGATAGCATTGAGGACATTCCTGACGAACTCCTCGGTTCCACCAGATCGGAACAAGAATACTGA

Upstream 100 bases:

>100_bases
GGTTCAAAACAAGGTACGAATATATATACACCTTGCGACAAGAAGAAGGAAGCCGAGTTGGATTTTGGCAGATTAAAAAC
TTACTCGTAAAGGTAAAAAA

Downstream 100 bases:

>100_bases
TTTGATTCGCTAAACAGGGTTTATGTGAAGATTAAACCAAATGAAATTCAACTTAGTATTGTTTTGTACAAATTTGAAGA
ACCTCCCGTTTAGAATTAAA

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTEILSQDEIDALLNAISSGEVSEDEYSSVGEQKKVKIYDFKRPDKFSKDQIRTLQMMHEIFARLATTGLSAQLRALVVV
HVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEIDPSISFTIIDRLFGGKGESSKVNRELSDIELSVMEGIIVRI
LGNLRESWSTVIDLRPRLGNIETNPQFAQVVPPNDMVVLITLETKVGEVERMTNLCIPYITIEPIINKLSAQYWYSSIRK
GEVDENRAVIQERLDQVKIPLISEVGSVDISLNDLMNLHVGDVIKLENTPIKTDLMVKVGDRSKFKATPGRVGNRLAIQI
GDSIEDIPDELLGSTRSEQEY

Sequences:

>Translated_341_residues
MTEILSQDEIDALLNAISSGEVSEDEYSSVGEQKKVKIYDFKRPDKFSKDQIRTLQMMHEIFARLATTGLSAQLRALVVV
HVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEIDPSISFTIIDRLFGGKGESSKVNRELSDIELSVMEGIIVRI
LGNLRESWSTVIDLRPRLGNIETNPQFAQVVPPNDMVVLITLETKVGEVERMTNLCIPYITIEPIINKLSAQYWYSSIRK
GEVDENRAVIQERLDQVKIPLISEVGSVDISLNDLMNLHVGDVIKLENTPIKTDLMVKVGDRSKFKATPGRVGNRLAIQI
GDSIEDIPDELLGSTRSEQEY
>Mature_340_residues
TEILSQDEIDALLNAISSGEVSEDEYSSVGEQKKVKIYDFKRPDKFSKDQIRTLQMMHEIFARLATTGLSAQLRALVVVH
VASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEIDPSISFTIIDRLFGGKGESSKVNRELSDIELSVMEGIIVRIL
GNLRESWSTVIDLRPRLGNIETNPQFAQVVPPNDMVVLITLETKVGEVERMTNLCIPYITIEPIINKLSAQYWYSSIRKG
EVDENRAVIQERLDQVKIPLISEVGSVDISLNDLMNLHVGDVIKLENTPIKTDLMVKVGDRSKFKATPGRVGNRLAIQIG
DSIEDIPDELLGSTRSEQEY

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=287, Percent_Identity=29.2682926829268, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 38154; Mature: 38023

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEILSQDEIDALLNAISSGEVSEDEYSSVGEQKKVKIYDFKRPDKFSKDQIRTLQMMHE
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
IFARLATTGLSAQLRALVVVHVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEID
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEC
PSISFTIIDRLFGGKGESSKVNRELSDIELSVMEGIIVRILGNLRESWSTVIDLRPRLGN
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
IETNPQFAQVVPPNDMVVLITLETKVGEVERMTNLCIPYITIEPIINKLSAQYWYSSIRK
CCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCC
GEVDENRAVIQERLDQVKIPLISEVGSVDISLNDLMNLHVGDVIKLENTPIKTDLMVKVG
CCCCHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHHCCCCCEEEECCCCCCEEEEEEEC
DRSKFKATPGRVGNRLAIQIGDSIEDIPDELLGSTRSEQEY
CCCCCCCCCCCCCCEEEEEECCCHHHCHHHHHCCCCCCCCC
>Mature Secondary Structure 
TEILSQDEIDALLNAISSGEVSEDEYSSVGEQKKVKIYDFKRPDKFSKDQIRTLQMMHE
CCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
IFARLATTGLSAQLRALVVVHVASVDQLTYEEFIRSIPNPTTLAVINMDPLRGSAILEID
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEC
PSISFTIIDRLFGGKGESSKVNRELSDIELSVMEGIIVRILGNLRESWSTVIDLRPRLGN
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
IETNPQFAQVVPPNDMVVLITLETKVGEVERMTNLCIPYITIEPIINKLSAQYWYSSIRK
CCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHCC
GEVDENRAVIQERLDQVKIPLISEVGSVDISLNDLMNLHVGDVIKLENTPIKTDLMVKVG
CCCCHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHHCCCCCEEEECCCCCCEEEEEEEC
DRSKFKATPGRVGNRLAIQIGDSIEDIPDELLGSTRSEQEY
CCCCCCCCCCCCCCEEEEEECCCHHHCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755894; 9665876; 8529894 [H]