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The map label for this gene is metY [H]

Identifier: 183220980

GI number: 183220980

Start: 1669494

End: 1670798

Strand: Reverse

Name: metY [H]

Synonym: LEPBI_I1593

Alternate gene names: 183220980

Gene position: 1670798-1669494 (Counterclockwise)

Preceding gene: 183220982

Following gene: 183220979

Centisome position: 46.42

GC content: 42.53

Gene sequence:

>1305_bases
ATGCCACGCCAATACAAACCAGAAACCATCGCACTTCACGGAGGCCAAGAGCCTGATCCAACCACTACGTCAAGAGCGGT
CCCACTTTACCAAACGACATCGTATGTTTTCAAAGATACAGACCATGCGGCCAGGTTATTTGGGCTCCAAGAGTTTGGAA
ATATTTATACCAGACTCATGAATCCAACAACGGATGTTTTGGAAAAACGTGTGGCAGCTTTAGAAGGTGGGGTGGCAGCA
CTTGCGACCGCTTCTGGCCAAAGTGCTGAAATGCTTGCCCTGTTGAATATTGTAGAAGCAGGTCAAGAAATTGTCGCCTC
TTCTTCGTTATATGGTGGAACTTATAATTTACTCCATTATACATTTCCAAAATTAGGAATCAAGGTTCATTTTGTAGACC
CATCCAACCCAGAAAATTTCAAAAAAGCATCGAATGATAAAACACGTGCCTTTTATGCTGAGACATTAGGAAACCCAAAA
TTAGATACACTTGACATAGCAGCCGTTAGTAAGGTCGCAAAAGAAGTTGGTGTACCACTTGTGATCGACAACACAATGCC
TTCTCCTTTTTTAGTGAATCCTCTCAAACATGGAGCAGATATTGTTGTTCACTCATTAACGAAATTTTTAGGAGGCCATG
GAACATCGATTGGAGGCATCATCATCGATGGTGGTAGTTTCAATTGGGGGAATGGCAAATTTAAAAATTTTACGGAACCA
GATCCTTCCTACCATGGACTTAAATTTTGGGATGTGTTTGGGAAATTTGAACCGTTTGGTGGAGTGAACATTGCGTTCAT
TCTAAAGGCTAGAGTGCAAGGATTACGAGATTTAGGACCAGCGATTTCTCCATTTAACGCATGGCAAATTCTACAAGGTG
TGGAAACACTCCCCCTTAGAATGGAACGCCACTCCCACAATGCATTGAAAGTAGCAGAATTCCTACAAAAACATCCTAAG
GTCGAATGGGTGAATTACCCTGGTCTACCGACTGACAAAAACTACGCTACTGCGAAAAAATACCATGAACGTGGACTATT
CGGTGCGATCGTAGGTTTCGAAATCAAAGGTGGTGTTGAGAAGGCAAAAAAATTCATCGATGGTTTGGAACTATTTAGTC
TCCTTGCTAACATCGGTGATGCGAAATCACTTGCCATCCACCCGGCATCAACCACCCACCAACAATTAACAAGTGAGGAA
CAAATCTCGGCTGGTGTCACTCCTGGGTTTGTGAGACTCAGTGTCGGTTTAGAAAATCTAGACGACATTCTAGTAGACTT
GGAAGAGGCATTAAAAAATATCTGA

Upstream 100 bases:

>100_bases
GGACAAATCCAATAAAACAGATATTTATTCTATTTTATTGGAGATTTTATTGACAAATGAGAATGCGAAAGGATAGTGGA
CGATACCATCGGGGGAATCT

Downstream 100 bases:

>100_bases
TTAAAGACATGCCTACTTCAGAACCAAACGATTTTTTTCATGGATCCGTAGGCATCGTACAGACTCAGGTAGCCACGTTT
GACTCATTAACACTTGAGGG

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPRQYKPETIALHGGQEPDPTTTSRAVPLYQTTSYVFKDTDHAARLFGLQEFGNIYTRLMNPTTDVLEKRVAALEGGVAA
LATASGQSAEMLALLNIVEAGQEIVASSSLYGGTYNLLHYTFPKLGIKVHFVDPSNPENFKKASNDKTRAFYAETLGNPK
LDTLDIAAVSKVAKEVGVPLVIDNTMPSPFLVNPLKHGADIVVHSLTKFLGGHGTSIGGIIIDGGSFNWGNGKFKNFTEP
DPSYHGLKFWDVFGKFEPFGGVNIAFILKARVQGLRDLGPAISPFNAWQILQGVETLPLRMERHSHNALKVAEFLQKHPK
VEWVNYPGLPTDKNYATAKKYHERGLFGAIVGFEIKGGVEKAKKFIDGLELFSLLANIGDAKSLAIHPASTTHQQLTSEE
QISAGVTPGFVRLSVGLENLDDILVDLEEALKNI

Sequences:

>Translated_434_residues
MPRQYKPETIALHGGQEPDPTTTSRAVPLYQTTSYVFKDTDHAARLFGLQEFGNIYTRLMNPTTDVLEKRVAALEGGVAA
LATASGQSAEMLALLNIVEAGQEIVASSSLYGGTYNLLHYTFPKLGIKVHFVDPSNPENFKKASNDKTRAFYAETLGNPK
LDTLDIAAVSKVAKEVGVPLVIDNTMPSPFLVNPLKHGADIVVHSLTKFLGGHGTSIGGIIIDGGSFNWGNGKFKNFTEP
DPSYHGLKFWDVFGKFEPFGGVNIAFILKARVQGLRDLGPAISPFNAWQILQGVETLPLRMERHSHNALKVAEFLQKHPK
VEWVNYPGLPTDKNYATAKKYHERGLFGAIVGFEIKGGVEKAKKFIDGLELFSLLANIGDAKSLAIHPASTTHQQLTSEE
QISAGVTPGFVRLSVGLENLDDILVDLEEALKNI
>Mature_433_residues
PRQYKPETIALHGGQEPDPTTTSRAVPLYQTTSYVFKDTDHAARLFGLQEFGNIYTRLMNPTTDVLEKRVAALEGGVAAL
ATASGQSAEMLALLNIVEAGQEIVASSSLYGGTYNLLHYTFPKLGIKVHFVDPSNPENFKKASNDKTRAFYAETLGNPKL
DTLDIAAVSKVAKEVGVPLVIDNTMPSPFLVNPLKHGADIVVHSLTKFLGGHGTSIGGIIIDGGSFNWGNGKFKNFTEPD
PSYHGLKFWDVFGKFEPFGGVNIAFILKARVQGLRDLGPAISPFNAWQILQGVETLPLRMERHSHNALKVAEFLQKHPKV
EWVNYPGLPTDKNYATAKKYHERGLFGAIVGFEIKGGVEKAKKFIDGLELFSLLANIGDAKSLAIHPASTTHQQLTSEEQ
ISAGVTPGFVRLSVGLENLDDILVDLEEALKNI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=426, Percent_Identity=33.5680751173709, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI299473759, Length=426, Percent_Identity=30.9859154929577, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI25453487, Length=424, Percent_Identity=30.188679245283, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1790375, Length=428, Percent_Identity=30.607476635514, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789383, Length=437, Percent_Identity=23.1121281464531, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71980708, Length=414, Percent_Identity=31.1594202898551, Blast_Score=211, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17538055, Length=427, Percent_Identity=32.3185011709602, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=427, Percent_Identity=32.7868852459016, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=449, Percent_Identity=45.2115812917595, Blast_Score=352, Evalue=5e-98,
Organism=Saccharomyces cerevisiae, GI6319307, Length=382, Percent_Identity=34.2931937172775, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=450, Percent_Identity=25.7777777777778, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=364, Percent_Identity=26.0989010989011, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=437, Percent_Identity=22.1967963386728, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922554, Length=429, Percent_Identity=32.1678321678322, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47294; Mature: 47163

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQYKPETIALHGGQEPDPTTTSRAVPLYQTTSYVFKDTDHAARLFGLQEFGNIYTRLM
CCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCHHEECCCCHHHHHHHHHHHHHHHHHHC
NPTTDVLEKRVAALEGGVAALATASGQSAEMLALLNIVEAGQEIVASSSLYGGTYNLLHY
CCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHE
TFPKLGIKVHFVDPSNPENFKKASNDKTRAFYAETLGNPKLDTLDIAAVSKVAKEVGVPL
ECCCCCEEEEEECCCCCHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCE
VIDNTMPSPFLVNPLKHGADIVVHSLTKFLGGHGTSIGGIIIDGGSFNWGNGKFKNFTEP
EECCCCCCCCEECHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
DPSYHGLKFWDVFGKFEPFGGVNIAFILKARVQGLRDLGPAISPFNAWQILQGVETLPLR
CCCCCCEEHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHCCHH
MERHSHNALKVAEFLQKHPKVEWVNYPGLPTDKNYATAKKYHERGLFGAIVGFEIKGGVE
HHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCHH
KAKKFIDGLELFSLLANIGDAKSLAIHPASTTHQQLTSEEQISAGVTPGFVRLSVGLENL
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCHHHHHCCCCCCEEEEEECHHHH
DDILVDLEEALKNI
HHHHHHHHHHHHCC
>Mature Secondary Structure 
PRQYKPETIALHGGQEPDPTTTSRAVPLYQTTSYVFKDTDHAARLFGLQEFGNIYTRLM
CCCCCCCEEEEECCCCCCCCCCCCCEEEEECCHHEECCCCHHHHHHHHHHHHHHHHHHC
NPTTDVLEKRVAALEGGVAALATASGQSAEMLALLNIVEAGQEIVASSSLYGGTYNLLHY
CCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHE
TFPKLGIKVHFVDPSNPENFKKASNDKTRAFYAETLGNPKLDTLDIAAVSKVAKEVGVPL
ECCCCCEEEEEECCCCCHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCE
VIDNTMPSPFLVNPLKHGADIVVHSLTKFLGGHGTSIGGIIIDGGSFNWGNGKFKNFTEP
EECCCCCCCCEECHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
DPSYHGLKFWDVFGKFEPFGGVNIAFILKARVQGLRDLGPAISPFNAWQILQGVETLPLR
CCCCCCEEHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHCCHH
MERHSHNALKVAEFLQKHPKVEWVNYPGLPTDKNYATAKKYHERGLFGAIVGFEIKGGVE
HHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCHH
KAKKFIDGLELFSLLANIGDAKSLAIHPASTTHQQLTSEEQISAGVTPGFVRLSVGLENL
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCHHHHHCCCCCCEEEEEECHHHH
DDILVDLEEALKNI
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]