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The map label for this gene is rpfG [H]

Identifier: 183220936

GI number: 183220936

Start: 1616701

End: 1617702

Strand: Direct

Name: rpfG [H]

Synonym: LEPBI_I1549

Alternate gene names: 183220936

Gene position: 1616701-1617702 (Clockwise)

Preceding gene: 183220935

Following gene: 183220939

Centisome position: 44.91

GC content: 37.33

Gene sequence:

>1002_bases
ATGATTCAAAACACAAAACCTAAAATTTTAGTCATTGATGATGAAGCGACGAACTTACAAATATTAAATGAAATTTTGAA
GAACGATTATTCCTTAAGTTTTGCAAAAGATGCAAATAAAGGATGGGAACTGGCCTTTAGTGAATCTCCCGATTTGATTT
TGCTTGATGTGATGATGCCTGAAAAAACAGGTTATGACCTCATCAAACAATTAAAGGAAAATAAAAAAACTCAGTTCATC
CCAGTGATTTTTGTAACAGCACTTACAGATGTAGGTGATGAGGAAAAAGGATTTCATTTGGGCGCAGTGGATTATATTAC
AAAACCTGTAAGCCCCGCAATCGTCAAAGCGAGGGTGAAAACACATTTATCGCTTGTGGATAGTACGGAAGTCAAGATCA
CAAGATTACAGATCATCCAGAGATTAGGGATGGCTTCCGAATACAAAGATAATGAAACGGGAATGCATGTCATTCGGATG
AGCCATTATAGCAAAACACTTGCTCTCGCCATTGGTTTTAGTGCAGAAGGTGCAGATGAAATTTTAAATGCAGCACCCAT
GCACGATGTAGGTAAAATTGGAATCCCAGATTCGATCATCCAAAAACCAGGTAAACTCACGGAAGAAGAATGGAATATCA
TGAAACGCCATCCTGAAATTGGAGCCGAAATCATCGGAGACCATCATTCCCGTTTGTTAAAATTAGCAAAGTCCATCGCT
CTCACACATCATGAAAAATATGATGGATCTGGATATCCTTATCAGTTAAAAGGGGAAAACATTCCTTTGGAAGGAAGGAT
CATCTCCATTGCCGATGTATTTGATGCCCTAACAACCATAAGACCATATAAAAAAGCATGGGAAGTAGAAGACGCTTTAG
GATATTTACAAAAAGAATCAGGTACACATTTTGATCCAAAATTGGTAAACGAGTTTTTGAAGGTTCTTCCGAAGATTCTT
GAAATCAAAGCAGAATGGCCGGAAGAAATTCAAAAAAAATAA

Upstream 100 bases:

>100_bases
TTGGAATTAGATTCGGTATCCAAATCGATCGAACAATTTGATTTTGAAAATTCGATCCTACTTTTAGAGAATTTAGAACA
AAAAATGAGAGAGCATTCAA

Downstream 100 bases:

>100_bases
ATTCAAATTCGATTTTGAATCCAAACGTTTTTTTTCGCATTGGAAATTGAAGGTTTACGTTAGTAAGCTTTGAATCCTGT
TGGCCTTTCTCCTCGTTTGT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MIQNTKPKILVIDDEATNLQILNEILKNDYSLSFAKDANKGWELAFSESPDLILLDVMMPEKTGYDLIKQLKENKKTQFI
PVIFVTALTDVGDEEKGFHLGAVDYITKPVSPAIVKARVKTHLSLVDSTEVKITRLQIIQRLGMASEYKDNETGMHVIRM
SHYSKTLALAIGFSAEGADEILNAAPMHDVGKIGIPDSIIQKPGKLTEEEWNIMKRHPEIGAEIIGDHHSRLLKLAKSIA
LTHHEKYDGSGYPYQLKGENIPLEGRIISIADVFDALTTIRPYKKAWEVEDALGYLQKESGTHFDPKLVNEFLKVLPKIL
EIKAEWPEEIQKK

Sequences:

>Translated_333_residues
MIQNTKPKILVIDDEATNLQILNEILKNDYSLSFAKDANKGWELAFSESPDLILLDVMMPEKTGYDLIKQLKENKKTQFI
PVIFVTALTDVGDEEKGFHLGAVDYITKPVSPAIVKARVKTHLSLVDSTEVKITRLQIIQRLGMASEYKDNETGMHVIRM
SHYSKTLALAIGFSAEGADEILNAAPMHDVGKIGIPDSIIQKPGKLTEEEWNIMKRHPEIGAEIIGDHHSRLLKLAKSIA
LTHHEKYDGSGYPYQLKGENIPLEGRIISIADVFDALTTIRPYKKAWEVEDALGYLQKESGTHFDPKLVNEFLKVLPKIL
EIKAEWPEEIQKK
>Mature_333_residues
MIQNTKPKILVIDDEATNLQILNEILKNDYSLSFAKDANKGWELAFSESPDLILLDVMMPEKTGYDLIKQLKENKKTQFI
PVIFVTALTDVGDEEKGFHLGAVDYITKPVSPAIVKARVKTHLSLVDSTEVKITRLQIIQRLGMASEYKDNETGMHVIRM
SHYSKTLALAIGFSAEGADEILNAAPMHDVGKIGIPDSIIQKPGKLTEEEWNIMKRHPEIGAEIIGDHHSRLLKLAKSIA
LTHHEKYDGSGYPYQLKGENIPLEGRIISIADVFDALTTIRPYKKAWEVEDALGYLQKESGTHFDPKLVNEFLKVLPKIL
EIKAEWPEEIQKK

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=139, Percent_Identity=34.5323741007194, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=34.1880341880342, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788394, Length=123, Percent_Identity=36.5853658536585, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=34.1880341880342, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 37492; Mature: 37492

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQNTKPKILVIDDEATNLQILNEILKNDYSLSFAKDANKGWELAFSESPDLILLDVMMP
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCEEEEEEECC
EKTGYDLIKQLKENKKTQFIPVIFVTALTDVGDEEKGFHLGAVDYITKPVSPAIVKARVK
CCCCHHHHHHHHHCCCCEEHHHHHEEHHHCCCCCCCCEEECHHHHHHCCCCHHHHHHHHH
THLSLVDSTEVKITRLQIIQRLGMASEYKDNETGMHVIRMSHYSKTLALAIGFSAEGADE
HHHHHHCCCCHHHHHHHHHHHHCCCHHCCCCCCCEEEEEEHHCCCEEEEEEECCCCCHHH
ILNAAPMHDVGKIGIPDSIIQKPGKLTEEEWNIMKRHPEIGAEIIGDHHSRLLKLAKSIA
HHHCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LTHHEKYDGSGYPYQLKGENIPLEGRIISIADVFDALTTIRPYKKAWEVEDALGYLQKES
HHHCCCCCCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTHFDPKLVNEFLKVLPKILEIKAEWPEEIQKK
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure
MIQNTKPKILVIDDEATNLQILNEILKNDYSLSFAKDANKGWELAFSESPDLILLDVMMP
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCEEEEEEECC
EKTGYDLIKQLKENKKTQFIPVIFVTALTDVGDEEKGFHLGAVDYITKPVSPAIVKARVK
CCCCHHHHHHHHHCCCCEEHHHHHEEHHHCCCCCCCCEEECHHHHHHCCCCHHHHHHHHH
THLSLVDSTEVKITRLQIIQRLGMASEYKDNETGMHVIRMSHYSKTLALAIGFSAEGADE
HHHHHHCCCCHHHHHHHHHHHHCCCHHCCCCCCCEEEEEEHHCCCEEEEEEECCCCCHHH
ILNAAPMHDVGKIGIPDSIIQKPGKLTEEEWNIMKRHPEIGAEIIGDHHSRLLKLAKSIA
HHHCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LTHHEKYDGSGYPYQLKGENIPLEGRIISIADVFDALTTIRPYKKAWEVEDALGYLQKES
HHHCCCCCCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTHFDPKLVNEFLKVLPKILEIKAEWPEEIQKK
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]