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The map label for this gene is smpB [H]

Identifier: 183220800

GI number: 183220800

Start: 1471966

End: 1472454

Strand: Direct

Name: smpB [H]

Synonym: LEPBI_I1411

Alternate gene names: 183220800

Gene position: 1471966-1472454 (Clockwise)

Preceding gene: 183220799

Following gene: 183220801

Centisome position: 40.89

GC content: 39.26

Gene sequence:

>489_bases
ATGGGCAAAACAAAAAAAGACGATAAACCCCGCGGAACTGATCCTTTGATCAATAAAAAGGCAAAGTTCAATTTCGAACT
ACTCGATTCGTTCGAGGCGGGAGTTGTACTCACAGGATCTGAGGTGAAATCCCTTCGTGAAAAAAAAGGAAACCTCACCG
ATTGTTTTGCCAAAGTGAGAAATGGGGAAGTATTTTTAGAAAATTTTCAAATCCCTCCTTACAAAAACGGAGGTTATGCG
AACCACCCTGAAATTCGTCCAAGAAAACTCCTGCTCAAGGCAAAAGAAATTGAAAAAATAGATCGTTCGATAAAAGAGAA
GGGACTTGTGCTTGTTGCCACTCGTTGTTTCTTTAAGAACAATCGTTTGGTGAAGATTGATGTCGCATTAGCCAAACCAA
AAAAATTATACGACAAACGGGATGATATCCAAAAAAAGGAAGCCAAAATCGATATGGAAAGAGCCATGAAGGAACACTTA
CGCAAATGA

Upstream 100 bases:

>100_bases
TCCAAATCACGGCGGATAATTTTTATTCGATGCCAAATCTCATTGGAGCTGAAATTGCGAGACTCGTGCAAATCCACCAT
TCCTTGAGACAGTAATCAGT

Downstream 100 bases:

>100_bases
AGGGACTTCCAGTGGTCACCATTGTTGGTAGACAAAATGTGGGAAAATCCACATTATTTAACGCCATTCTCCGAGCACAA
AGTGCGATTACCGAAAACAC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MGKTKKDDKPRGTDPLINKKAKFNFELLDSFEAGVVLTGSEVKSLREKKGNLTDCFAKVRNGEVFLENFQIPPYKNGGYA
NHPEIRPRKLLLKAKEIEKIDRSIKEKGLVLVATRCFFKNNRLVKIDVALAKPKKLYDKRDDIQKKEAKIDMERAMKEHL
RK

Sequences:

>Translated_162_residues
MGKTKKDDKPRGTDPLINKKAKFNFELLDSFEAGVVLTGSEVKSLREKKGNLTDCFAKVRNGEVFLENFQIPPYKNGGYA
NHPEIRPRKLLLKAKEIEKIDRSIKEKGLVLVATRCFFKNNRLVKIDVALAKPKKLYDKRDDIQKKEAKIDMERAMKEHL
RK
>Mature_161_residues
GKTKKDDKPRGTDPLINKKAKFNFELLDSFEAGVVLTGSEVKSLREKKGNLTDCFAKVRNGEVFLENFQIPPYKNGGYAN
HPEIRPRKLLLKAKEIEKIDRSIKEKGLVLVATRCFFKNNRLVKIDVALAKPKKLYDKRDDIQKKEAKIDMERAMKEHLR
K

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=158, Percent_Identity=36.0759493670886, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18701; Mature: 18570

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTKKDDKPRGTDPLINKKAKFNFELLDSFEAGVVLTGSEVKSLREKKGNLTDCFAKVR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHC
NGEVFLENFQIPPYKNGGYANHPEIRPRKLLLKAKEIEKIDRSIKEKGLVLVATRCFFKN
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHCC
NRLVKIDVALAKPKKLYDKRDDIQKKEAKIDMERAMKEHLRK
CCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKTKKDDKPRGTDPLINKKAKFNFELLDSFEAGVVLTGSEVKSLREKKGNLTDCFAKVR
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEEECHHHHHHHHHCCCHHHHHHHHC
NGEVFLENFQIPPYKNGGYANHPEIRPRKLLLKAKEIEKIDRSIKEKGLVLVATRCFFKN
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHCC
NRLVKIDVALAKPKKLYDKRDDIQKKEAKIDMERAMKEHLRK
CCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA