Click here to switch to the map view.

The map label for this gene is ribBA [H]

Identifier: 183220795

GI number: 183220795

Start: 1464975

End: 1466183

Strand: Direct

Name: ribBA [H]

Synonym: LEPBI_I1406

Alternate gene names: 183220795

Gene position: 1464975-1466183 (Clockwise)

Preceding gene: 183220793

Following gene: 183220799

Centisome position: 40.7

GC content: 42.85

Gene sequence:

>1209_bases
ATGATACGTCCGATCGAAGAAGCAATCGAAGAAATCCGCCAAGGCAAAATGATCATCCTCGTCGACTCCGAAGATAGAGA
AAACGAAGGCGATTTGGTCTGTGCCTCCCAATTTGCGGACAAAGACAAAATCAATTTTATGGCAACCCATGGCAGGGGGC
TCATTTGTGTTCCTATGGAAAGAGACAGGCTTCAAAGTTTAGGCCTCGGGAAGATGGTAGATGACTTATCTCTTGGCGAT
AAACACGGAACTGCTTTTACCATATCAGTGGATGCAAAATTTGGTACCTCCACTGGCATTTCGGCGCATGACAGAGCCAA
AACTGTCGAAGTACTACTCGATCCTAATACCAAACCAGATGACCTCATGCGACCTGGACATTTATTTCCCTTGCAAGCGG
TAACAGGTGGTGTGTTACGAAGGGCAGGGCATACGGAAGCAGCAGTTGACTTATCAAAGTTAGCTGGTCTTTATCCGAGT
GGTGTTATTTGTGAAATTATGAACGACGATGGTTCCATGGCAAGGATTCCTGATTTGGAAAAATTTGCAAAAACACATGG
ACTGAATATTTATACAATTGAAGACCTCATTCGATACCGCAGGCATAAAGAAAAACTCATCCACTTAGAAGTAGAAGCAA
ACCTTCCGACTGAGTTTGGTGATTTTAAAATCAAAGCATACTCTACCCAAATTGATGACAAAATCCATATGGCACTTGTA
AAGGGAGACATCAATCCCGACAAACCAGTGCTTGTTCGTGTTCATAGTGAATGTTTGACGGGAGATATTTTTTCCTCTCA
ACGTTGTGATTGCGGACCACAACTCCACAATGCTCTTCGCATGATTGAAAAAGAAGGGAATGGTGTACTTTTGTACATGC
GACAAGAAGGGCGTGGGATTGGGATCATCAACAAACTCAAAGCCTATTCATTACAAGAAGGTGGCCTTGATACAGTGGAG
GCCAATGAAAAACTAGGATTTGCGCCTGACTTACGGGAGTATGGGATTGGAGCTCAAATCCTTCGTGACATAGGTGTGAA
ACAAATGAAACTCATCACAAATAACCCACGTAAGATTGTAGGCCTTGAAGGATACAACCTCCATGTGACCGAACGGGTTC
CCATTGAAATTGATCCAATCGAAGAGAACTCAAAATACTTACAGACCAAAAAAACAAAACTGGGACATCTTTTGAACTTA
CACGGTTAA

Upstream 100 bases:

>100_bases
CATTTAGACAAAAGAAAAGGGAAAAATCAATGAATTTCTTTGGGAATCTTCTTTAATTCGGAACGGCTTTCAGAGTTTTA
GTATATAGAGGCCTTTCCCT

Downstream 100 bases:

>100_bases
ACGTTTTTCTGCGTTAAATTCCAATGTTCGAATTTGAGTAAATGTTTTCAAAAGAGACATTCATTTAAGGCGATAGTCTC
GACCGAATCCAATTTTCGTT

Product: riboflavin biosynthesis protein RibA

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MIRPIEEAIEEIRQGKMIILVDSEDRENEGDLVCASQFADKDKINFMATHGRGLICVPMERDRLQSLGLGKMVDDLSLGD
KHGTAFTISVDAKFGTSTGISAHDRAKTVEVLLDPNTKPDDLMRPGHLFPLQAVTGGVLRRAGHTEAAVDLSKLAGLYPS
GVICEIMNDDGSMARIPDLEKFAKTHGLNIYTIEDLIRYRRHKEKLIHLEVEANLPTEFGDFKIKAYSTQIDDKIHMALV
KGDINPDKPVLVRVHSECLTGDIFSSQRCDCGPQLHNALRMIEKEGNGVLLYMRQEGRGIGIINKLKAYSLQEGGLDTVE
ANEKLGFAPDLREYGIGAQILRDIGVKQMKLITNNPRKIVGLEGYNLHVTERVPIEIDPIEENSKYLQTKKTKLGHLLNL
HG

Sequences:

>Translated_402_residues
MIRPIEEAIEEIRQGKMIILVDSEDRENEGDLVCASQFADKDKINFMATHGRGLICVPMERDRLQSLGLGKMVDDLSLGD
KHGTAFTISVDAKFGTSTGISAHDRAKTVEVLLDPNTKPDDLMRPGHLFPLQAVTGGVLRRAGHTEAAVDLSKLAGLYPS
GVICEIMNDDGSMARIPDLEKFAKTHGLNIYTIEDLIRYRRHKEKLIHLEVEANLPTEFGDFKIKAYSTQIDDKIHMALV
KGDINPDKPVLVRVHSECLTGDIFSSQRCDCGPQLHNALRMIEKEGNGVLLYMRQEGRGIGIINKLKAYSLQEGGLDTVE
ANEKLGFAPDLREYGIGAQILRDIGVKQMKLITNNPRKIVGLEGYNLHVTERVPIEIDPIEENSKYLQTKKTKLGHLLNL
HG
>Mature_402_residues
MIRPIEEAIEEIRQGKMIILVDSEDRENEGDLVCASQFADKDKINFMATHGRGLICVPMERDRLQSLGLGKMVDDLSLGD
KHGTAFTISVDAKFGTSTGISAHDRAKTVEVLLDPNTKPDDLMRPGHLFPLQAVTGGVLRRAGHTEAAVDLSKLAGLYPS
GVICEIMNDDGSMARIPDLEKFAKTHGLNIYTIEDLIRYRRHKEKLIHLEVEANLPTEFGDFKIKAYSTQIDDKIHMALV
KGDINPDKPVLVRVHSECLTGDIFSSQRCDCGPQLHNALRMIEKEGNGVLLYMRQEGRGIGIINKLKAYSLQEGGLDTVE
ANEKLGFAPDLREYGIGAQILRDIGVKQMKLITNNPRKIVGLEGYNLHVTERVPIEIDPIEENSKYLQTKKTKLGHLLNL
HG

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0807

COG function: function code H; GTP cyclohydrolase II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1787533, Length=189, Percent_Identity=50.7936507936508, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1789420, Length=201, Percent_Identity=43.7810945273632, Blast_Score=192, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6320695, Length=205, Percent_Identity=44.8780487804878, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6319438, Length=172, Percent_Identity=43.6046511627907, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 44692; Mature: 44692

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPIEEAIEEIRQGKMIILVDSEDRENEGDLVCASQFADKDKINFMATHGRGLICVPME
CCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHCCCCCCEEEEEECCCEEEEECCC
RDRLQSLGLGKMVDDLSLGDKHGTAFTISVDAKFGTSTGISAHDRAKTVEVLLDPNTKPD
HHHHHHCCCHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHH
DLMRPGHLFPLQAVTGGVLRRAGHTEAAVDLSKLAGLYPSGVICEIMNDDGSMARIPDLE
HHCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCHH
KFAKTHGLNIYTIEDLIRYRRHKEKLIHLEVEANLPTEFGDFKIKAYSTQIDDKIHMALV
HHHHHHCCEEEEHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCEEEEEEE
KGDINPDKPVLVRVHSECLTGDIFSSQRCDCGPQLHNALRMIEKEGNGVLLYMRQEGRGI
ECCCCCCCCEEEEEECHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCC
GIINKLKAYSLQEGGLDTVEANEKLGFAPDLREYGIGAQILRDIGVKQMKLITNNPRKIV
CCHHHHHHHEECCCCCCEEECCCCCCCCCCHHHHCCHHHHHHHCCHHEEEEEECCCCEEE
GLEGYNLHVTERVPIEIDPIEENSKYLQTKKTKLGHLLNLHG
EECCCEEEEEEECCEEECCCCCCCHHHHHHHHHHCCEEECCC
>Mature Secondary Structure
MIRPIEEAIEEIRQGKMIILVDSEDRENEGDLVCASQFADKDKINFMATHGRGLICVPME
CCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHCCCCCCEEEEEECCCEEEEECCC
RDRLQSLGLGKMVDDLSLGDKHGTAFTISVDAKFGTSTGISAHDRAKTVEVLLDPNTKPD
HHHHHHCCCHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHH
DLMRPGHLFPLQAVTGGVLRRAGHTEAAVDLSKLAGLYPSGVICEIMNDDGSMARIPDLE
HHCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCHH
KFAKTHGLNIYTIEDLIRYRRHKEKLIHLEVEANLPTEFGDFKIKAYSTQIDDKIHMALV
HHHHHHCCEEEEHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCEEEEEEE
KGDINPDKPVLVRVHSECLTGDIFSSQRCDCGPQLHNALRMIEKEGNGVLLYMRQEGRGI
ECCCCCCCCEEEEEECHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCC
GIINKLKAYSLQEGGLDTVEANEKLGFAPDLREYGIGAQILRDIGVKQMKLITNNPRKIV
CCHHHHHHHEECCCCCCEEECCCCCCCCCCHHHHCCHHHHHHHCCHHEEEEEECCCCEEE
GLEGYNLHVTERVPIEIDPIEENSKYLQTKKTKLGHLLNLHG
EECCCEEEEEEECCEEECCCCCCCHHHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA