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The map label for this gene is ysgA [H]

Identifier: 183220443

GI number: 183220443

Start: 1080462

End: 1081295

Strand: Reverse

Name: ysgA [H]

Synonym: LEPBI_I1040

Alternate gene names: 183220443

Gene position: 1081295-1080462 (Counterclockwise)

Preceding gene: 183220444

Following gene: 183220442

Centisome position: 30.04

GC content: 41.37

Gene sequence:

>834_bases
ATGAAACCACAAAGGAATTACATCACAAGTTTCTCCAATCCGAAGGTGAAATGGGTAGCAGGCCTCAAAGAAAAACGAAA
TCGTGATGAAGAAAAAAAATTTTTCATCGAAGGGTATCGGGAAATCAAAAAAGCCATTACGGGAAACCCAAACTCACCGA
TTCCTTGTTTGCCTGTGAACATCACAAGTTTATTCATTTCCCCTGAATGTTTTTTGGGAGAAAATGAAGAGAGTTTGATA
GCATCAATTCGTTGTCCTATCTTTGAACTCCCACGGAAAATTTTTGAAAAAATTTCCTACAGGGATCGTCCGGATGGTCT
TATCGCAGTCGCAGAGACTCCAAGCGCCATGGTTCCTTGGAACTCCCTTCAAACCAGTGAAACAAATCCTATCCTCATCA
TTGAAGGTGTGGAAAAACCTGGAAATCTCGGAACCATCCTACGCACGGCAGAAGGAGCAGGTGTGGGACTTGTGATTGTC
ACCGATCCAAGGATTGATCTTTTTAATCCAAATGTGGTGAGGGCAAGCACAGGAACAATTTTTACTCTTCCTGTTTACAT
TGGTGACCTAACAGAAGTTTTAACAGAGTTCAAAAAACAAGGTTACAAACGATATGCCGTCACCCCGGAAGGAAAAACTT
TATACACTTCGGTGAACCTAAAAGAAAAGTCAGTTTTTTTATTTGGAAGTGAGCAGTATGGACTTAGCCCACTTGCCAAA
GAACTCGCAGATACAACTTTGTATTTGCCTATGTTTGGTGAAGCGGATTCCCTCAATTTGGCTATGTCTTGTGGCATCGT
TTTGTATGAATCCATCCGCCAAAGATCCAAATGA

Upstream 100 bases:

>100_bases
ATACGAAACAACTGAACTTTATATCCCAGAAACTTCTGGGAAAAAATATCCGGCTGGATTCTGCACCTTTTTCAAACGTT
CCTAATCGAGTATTTCTCCA

Downstream 100 bases:

>100_bases
AAATTTATTATTCGATCTCAGGCCATGGTTTTGGTCACATCAGTCGTTCTGGAAACATCATTAAACGCTTATTAGATGAA
GATTTCATCGAAGAAATCCA

Product: putative rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKPQRNYITSFSNPKVKWVAGLKEKRNRDEEKKFFIEGYREIKKAITGNPNSPIPCLPVNITSLFISPECFLGENEESLI
ASIRCPIFELPRKIFEKISYRDRPDGLIAVAETPSAMVPWNSLQTSETNPILIIEGVEKPGNLGTILRTAEGAGVGLVIV
TDPRIDLFNPNVVRASTGTIFTLPVYIGDLTEVLTEFKKQGYKRYAVTPEGKTLYTSVNLKEKSVFLFGSEQYGLSPLAK
ELADTTLYLPMFGEADSLNLAMSCGIVLYESIRQRSK

Sequences:

>Translated_277_residues
MKPQRNYITSFSNPKVKWVAGLKEKRNRDEEKKFFIEGYREIKKAITGNPNSPIPCLPVNITSLFISPECFLGENEESLI
ASIRCPIFELPRKIFEKISYRDRPDGLIAVAETPSAMVPWNSLQTSETNPILIIEGVEKPGNLGTILRTAEGAGVGLVIV
TDPRIDLFNPNVVRASTGTIFTLPVYIGDLTEVLTEFKKQGYKRYAVTPEGKTLYTSVNLKEKSVFLFGSEQYGLSPLAK
ELADTTLYLPMFGEADSLNLAMSCGIVLYESIRQRSK
>Mature_277_residues
MKPQRNYITSFSNPKVKWVAGLKEKRNRDEEKKFFIEGYREIKKAITGNPNSPIPCLPVNITSLFISPECFLGENEESLI
ASIRCPIFELPRKIFEKISYRDRPDGLIAVAETPSAMVPWNSLQTSETNPILIIEGVEKPGNLGTILRTAEGAGVGLVIV
TDPRIDLFNPNVVRASTGTIFTLPVYIGDLTEVLTEFKKQGYKRYAVTPEGKTLYTSVNLKEKSVFLFGSEQYGLSPLAK
ELADTTLYLPMFGEADSLNLAMSCGIVLYESIRQRSK

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=148, Percent_Identity=31.7567567567568, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30908; Mature: 30908

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQRNYITSFSNPKVKWVAGLKEKRNRDEEKKFFIEGYREIKKAITGNPNSPIPCLPVN
CCCCCCCCCCCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
ITSLFISPECFLGENEESLIASIRCPIFELPRKIFEKISYRDRPDGLIAVAETPSAMVPW
EEEEEECCCEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCC
NSLQTSETNPILIIEGVEKPGNLGTILRTAEGAGVGLVIVTDPRIDLFNPNVVRASTGTI
CCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCEEECCCCEEEECCCCE
FTLPVYIGDLTEVLTEFKKQGYKRYAVTPEGKTLYTSVNLKEKSVFLFGSEQYGLSPLAK
EEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCCHHHH
ELADTTLYLPMFGEADSLNLAMSCGIVLYESIRQRSK
HHHHCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPQRNYITSFSNPKVKWVAGLKEKRNRDEEKKFFIEGYREIKKAITGNPNSPIPCLPVN
CCCCCCCCCCCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
ITSLFISPECFLGENEESLIASIRCPIFELPRKIFEKISYRDRPDGLIAVAETPSAMVPW
EEEEEECCCEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCC
NSLQTSETNPILIIEGVEKPGNLGTILRTAEGAGVGLVIVTDPRIDLFNPNVVRASTGTI
CCCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCEEECCCCEEEECCCCE
FTLPVYIGDLTEVLTEFKKQGYKRYAVTPEGKTLYTSVNLKEKSVFLFGSEQYGLSPLAK
EEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCCHHHH
ELADTTLYLPMFGEADSLNLAMSCGIVLYESIRQRSK
HHHHCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]