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The map label for this gene is dapL

Identifier: 183220431

GI number: 183220431

Start: 1069580

End: 1070806

Strand: Direct

Name: dapL

Synonym: LEPBI_I1028

Alternate gene names: 183220431

Gene position: 1069580-1070806 (Clockwise)

Preceding gene: 183220430

Following gene: 183220432

Centisome position: 29.71

GC content: 44.91

Gene sequence:

>1227_bases
ATGACTCAGATAAATGAAAACTATTTAAAATTAAAAGCGGGATACCTATTCCCTGAAATCGGAAGAAGGGTAAAAGCGTA
TTCCGATGCCAACCAAAATGCTAAGATCATCCGACTTGGAATCGGGGACGTGACCCTTCCACTCGCACCTACGATTGTGA
ATGCAATGGTGGATGCGGCCAAAGAAATGGGAAGTGCTGGTGGTTTCCATGGGTATGGACCTGAACAAGGTTATAGTTTT
CTCATCCAAAAGATCATCGCTCACGATTACACAGCACGTGGCGTTCAAATTGCAGAAGACGAAGTGTTCGTTTCCGATGG
TTCCAAATGTGACTGTGGGAATATCCAGGAAATCTTTTCTCTTGATAGTAAAATTGCCGTTGTGGATCCAGTCTACCCAG
TGTATGTCGATACGAACGTGATGGCAGGAAGAACTGGAGAAGTGGGGCCTGACGGGCGTTATGCAAATATCGTTTACATG
CCTGCAACGGAAGAAAACAATTTTGAACCGGATTTCCCAAAAGAAAAAGCAGACATCATTTACCTTTGTTACCCCAATAA
CCCAACAGGGATGGTGGCAACGAAAGCGCGTCTGACAGAATGGGTGAACTATGCCAAAAAAATGGGAAGTATCATCCTTT
ATGATTCGGCCTACGAATCCTTTATCCAAGACCCTGAAATTCCAAAATCCATTTATGAAATCCCAGGTGCCAAAGAAGTC
GCAATGGAATTTCGTTCCTTTTCCAAAACGGCAGGGTTTACAGGAACACGATGTGCCTACCTAGTGATCCCAAAAGATCT
CAAAGGAAAAACCAAATCGGGAGAAGAGGTGAGTTTTAATTCTCTCTGGAACCGCCGTCACACAACGAAGTTCAATGGAG
TCTCCTACATCACACAAAAAGGAGCGGAGGCTGTTTTTTCAGCCCAAGGCCAAGTGGAGATCAAAGAACAAATTTCCTAT
TATATGGAAAATGCCAAACTCATCCGCGAAGGGTTGGTCAAGGCAGGTTACACTGTCTTTGGGGGAACAAACGCTCCATA
CATTTGGCTAAAAACCCCCCGAGGTTTGAAATCCTGGGAATTTTTTGATGAATTGTTGGGAACCGCACAAGTGGTCGGTA
CCCCTGGATCTGGTTTTGGACCGGCGGGAGAAGGGTATTTCCGGCTTTCTGCCTTCGGAAAACGGGAAGATGTCATTTCT
GCCATCGAACGCATCCAAAAAATGTAA

Upstream 100 bases:

>100_bases
TTTTACTGTTGGACATGGGGCGAAGAAGATGTGTTATACGAACACGGGTATCATGAAGGATTTAGATCGAGAAAACTCAT
AGAGGAAACAAAAGAAGAAA

Downstream 100 bases:

>100_bases
AATTTAGTCCTGGTTTTTTCCATAGCTCCGATATATAAAACAAGGTAGAGCTATGGGAAAATGGAAATTCATCCTCTTTT
TTGCAATGGTTGTGGGTCAT

Product: L,L-diaminopimelate aminotransferase

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTQINENYLKLKAGYLFPEIGRRVKAYSDANQNAKIIRLGIGDVTLPLAPTIVNAMVDAAKEMGSAGGFHGYGPEQGYSF
LIQKIIAHDYTARGVQIAEDEVFVSDGSKCDCGNIQEIFSLDSKIAVVDPVYPVYVDTNVMAGRTGEVGPDGRYANIVYM
PATEENNFEPDFPKEKADIIYLCYPNNPTGMVATKARLTEWVNYAKKMGSIILYDSAYESFIQDPEIPKSIYEIPGAKEV
AMEFRSFSKTAGFTGTRCAYLVIPKDLKGKTKSGEEVSFNSLWNRRHTTKFNGVSYITQKGAEAVFSAQGQVEIKEQISY
YMENAKLIREGLVKAGYTVFGGTNAPYIWLKTPRGLKSWEFFDELLGTAQVVGTPGSGFGPAGEGYFRLSAFGKREDVIS
AIERIQKM

Sequences:

>Translated_408_residues
MTQINENYLKLKAGYLFPEIGRRVKAYSDANQNAKIIRLGIGDVTLPLAPTIVNAMVDAAKEMGSAGGFHGYGPEQGYSF
LIQKIIAHDYTARGVQIAEDEVFVSDGSKCDCGNIQEIFSLDSKIAVVDPVYPVYVDTNVMAGRTGEVGPDGRYANIVYM
PATEENNFEPDFPKEKADIIYLCYPNNPTGMVATKARLTEWVNYAKKMGSIILYDSAYESFIQDPEIPKSIYEIPGAKEV
AMEFRSFSKTAGFTGTRCAYLVIPKDLKGKTKSGEEVSFNSLWNRRHTTKFNGVSYITQKGAEAVFSAQGQVEIKEQISY
YMENAKLIREGLVKAGYTVFGGTNAPYIWLKTPRGLKSWEFFDELLGTAQVVGTPGSGFGPAGEGYFRLSAFGKREDVIS
AIERIQKM
>Mature_407_residues
TQINENYLKLKAGYLFPEIGRRVKAYSDANQNAKIIRLGIGDVTLPLAPTIVNAMVDAAKEMGSAGGFHGYGPEQGYSFL
IQKIIAHDYTARGVQIAEDEVFVSDGSKCDCGNIQEIFSLDSKIAVVDPVYPVYVDTNVMAGRTGEVGPDGRYANIVYMP
ATEENNFEPDFPKEKADIIYLCYPNNPTGMVATKARLTEWVNYAKKMGSIILYDSAYESFIQDPEIPKSIYEIPGAKEVA
MEFRSFSKTAGFTGTRCAYLVIPKDLKGKTKSGEEVSFNSLWNRRHTTKFNGVSYITQKGAEAVFSAQGQVEIKEQISYY
MENAKLIREGLVKAGYTVFGGTNAPYIWLKTPRGLKSWEFFDELLGTAQVVGTPGSGFGPAGEGYFRLSAFGKREDVISA
IERIQKM

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily

Homologues:

Organism=Escherichia coli, GI1788722, Length=389, Percent_Identity=25.706940874036, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPAT_LEPBA (B0SEH8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962099.1
- ProteinModelPortal:   B0SEH8
- SMR:   B0SEH8
- GeneID:   6387235
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0994
- HOGENOM:   HBG645860
- OMA:   VDLIYLC
- ProtClustDB:   PRK07590
- BioCyc:   LBIF355278:LBF_0994-MONOMER
- HAMAP:   MF_01642
- InterPro:   IPR004839
- InterPro:   IPR019942
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR03542

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.83

Molecular weight: Translated: 45076; Mature: 44945

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 42-42 BINDING 72-72 BINDING 75-75 BINDING 109-109 BINDING 132-132 BINDING 187-187 BINDING 187-187 BINDING 215-215 BINDING 218-218 BINDING 246-246 BINDING 248-248 BINDING 257-257 BINDING 292-292 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQINENYLKLKAGYLFPEIGRRVKAYSDANQNAKIIRLGIGDVTLPLAPTIVNAMVDAA
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCEECCHHHHHHHHHHHHH
KEMGSAGGFHGYGPEQGYSFLIQKIIAHDYTARGVQIAEDEVFVSDGSKCDCGNIQEIFS
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCCCCCCCCHHHHHC
LDSKIAVVDPVYPVYVDTNVMAGRTGEVGPDGRYANIVYMPATEENNFEPDFPKEKADII
CCCCEEEECCCEEEEEECCEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEE
YLCYPNNPTGMVATKARLTEWVNYAKKMGSIILYDSAYESFIQDPEIPKSIYEIPGAKEV
EEEECCCCCCEEEHHHHHHHHHHHHHHHCCEEEEECHHHHHHCCCCCCHHHHHCCCHHHH
AMEFRSFSKTAGFTGTRCAYLVIPKDLKGKTKSGEEVSFNSLWNRRHTTKFNGVSYITQK
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHHHCCCCCCCCCCHHHHHHH
GAEAVFSAQGQVEIKEQISYYMENAKLIREGLVKAGYTVFGGTNAPYIWLKTPRGLKSWE
CCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCHHH
FFDELLGTAQVVGTPGSGFGPAGEGYFRLSAFGKREDVISAIERIQKM
HHHHHHCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQINENYLKLKAGYLFPEIGRRVKAYSDANQNAKIIRLGIGDVTLPLAPTIVNAMVDAA
CCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCEECCHHHHHHHHHHHHH
KEMGSAGGFHGYGPEQGYSFLIQKIIAHDYTARGVQIAEDEVFVSDGSKCDCGNIQEIFS
HHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCCCCCCCCHHHHHC
LDSKIAVVDPVYPVYVDTNVMAGRTGEVGPDGRYANIVYMPATEENNFEPDFPKEKADII
CCCCEEEECCCEEEEEECCEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEE
YLCYPNNPTGMVATKARLTEWVNYAKKMGSIILYDSAYESFIQDPEIPKSIYEIPGAKEV
EEEECCCCCCEEEHHHHHHHHHHHHHHHCCEEEEECHHHHHHCCCCCCHHHHHCCCHHHH
AMEFRSFSKTAGFTGTRCAYLVIPKDLKGKTKSGEEVSFNSLWNRRHTTKFNGVSYITQK
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHHHCCCCCCCCCCHHHHHHH
GAEAVFSAQGQVEIKEQISYYMENAKLIREGLVKAGYTVFGGTNAPYIWLKTPRGLKSWE
CCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCHHH
FFDELLGTAQVVGTPGSGFGPAGEGYFRLSAFGKREDVISAIERIQKM
HHHHHHCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA