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The map label for this gene is sppA [H]

Identifier: 183220418

GI number: 183220418

Start: 1060737

End: 1061735

Strand: Reverse

Name: sppA [H]

Synonym: LEPBI_I1014

Alternate gene names: 183220418

Gene position: 1061735-1060737 (Counterclockwise)

Preceding gene: 183220421

Following gene: 183220417

Centisome position: 29.5

GC content: 41.84

Gene sequence:

>999_bases
ATGCCTTTAAGAAAGTTTGGTCTTATCCGTTCCGTCGTTCTTCTTTCGGTTCTTTTTACAGAATCATGTGTCATTGGGAA
TAGTATGAATCTTTTCCCTCCAACCGCAAAGTCTGAATTCGAAGAGAAACTCATCGCAGGAAAGGATCAAGAAAAAATCG
TAATTATCTCCATCGAAGGGATGATTTCTGACGAAGGAAAAGAAGCCTTTTTTGGCCCCTCTTCCGATTCCATGGTCGTC
CGAGTGAAAGAATCTTTGAAACGTGCCGAACGGGACCCTGATGTCAAAGGTGTGATTTTAAAAATCAACTCACCTGGCGG
AACGGTCACTGCGAGCGATATCATTTACCAAGAGATCAAAAAATTCAAAGAAAGAAAAGGGATCCCTGTTTTTGCAGGTT
TTATGGACACGGCTGCCAGTGGTGCTTATTACATCGCCATGGCGACGGATTCGATCGGTGCTCACCCAACGACTGTTACA
GGATCGGTGGGAGTCATCATGTCTGGGATCAATGTCAAAGAAGGATTGGATAAAATTGGAATCAAAGACCAATCCTTTAC
TTCTGGTCCAAACAAAGCACTTGGTTCACCGACAACGGAAATGACTGCGGAACAAAGAAAAATTTTACAATCCATCATTG
ATAGTTTGTATTCCCGATTTTTTGAAGTGGTCAAAAAAGGTCGCCCCAAAGTTTCCGAAGCAAGACTCAAGGAAATTTGT
GATGGAAGGATCTTTACGGCAGAACAAGCGCAAAAAGAAGGGATGATCGATTTTATCGGATACTTTGATAATTTTGTAAT
CGAACTCATGAAACACCCGAAGTATGAAGGAAACCCACAAGGTTCACCACGAATTGTGACTTACCAAAGAGGGAAAGTTC
CTGTCGAAAATATCTACCAAGCAACAGATGTGAAAGGGAACCCAATTTCATTTGGAATCGCAGATAAACTTCTAGGAACA
GGAACGAACGCGAAGTTTTTATATCTCTGGGATATCTAG

Upstream 100 bases:

>100_bases
GGGTTCTGTCTCAAACCTTGAGAATTTAGTTTTCAGTTGGTGCAAGATTTATCTCCCAAAAATGATTGAAGTCAGAGTTT
GCCATCTTAGAAAGGTTTGT

Downstream 100 bases:

>100_bases
TTTTTAAGGTTACCTAATTCATGTTATTCAATTCGATTCCGTATTTAATTTTATTTGCTCTCACATATTTGATCTATTGG
AACATTCCACAGAAGGGAAG

Product: signal peptide peptidase SppA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MPLRKFGLIRSVVLLSVLFTESCVIGNSMNLFPPTAKSEFEEKLIAGKDQEKIVIISIEGMISDEGKEAFFGPSSDSMVV
RVKESLKRAERDPDVKGVILKINSPGGTVTASDIIYQEIKKFKERKGIPVFAGFMDTAASGAYYIAMATDSIGAHPTTVT
GSVGVIMSGINVKEGLDKIGIKDQSFTSGPNKALGSPTTEMTAEQRKILQSIIDSLYSRFFEVVKKGRPKVSEARLKEIC
DGRIFTAEQAQKEGMIDFIGYFDNFVIELMKHPKYEGNPQGSPRIVTYQRGKVPVENIYQATDVKGNPISFGIADKLLGT
GTNAKFLYLWDI

Sequences:

>Translated_332_residues
MPLRKFGLIRSVVLLSVLFTESCVIGNSMNLFPPTAKSEFEEKLIAGKDQEKIVIISIEGMISDEGKEAFFGPSSDSMVV
RVKESLKRAERDPDVKGVILKINSPGGTVTASDIIYQEIKKFKERKGIPVFAGFMDTAASGAYYIAMATDSIGAHPTTVT
GSVGVIMSGINVKEGLDKIGIKDQSFTSGPNKALGSPTTEMTAEQRKILQSIIDSLYSRFFEVVKKGRPKVSEARLKEIC
DGRIFTAEQAQKEGMIDFIGYFDNFVIELMKHPKYEGNPQGSPRIVTYQRGKVPVENIYQATDVKGNPISFGIADKLLGT
GTNAKFLYLWDI
>Mature_331_residues
PLRKFGLIRSVVLLSVLFTESCVIGNSMNLFPPTAKSEFEEKLIAGKDQEKIVIISIEGMISDEGKEAFFGPSSDSMVVR
VKESLKRAERDPDVKGVILKINSPGGTVTASDIIYQEIKKFKERKGIPVFAGFMDTAASGAYYIAMATDSIGAHPTTVTG
SVGVIMSGINVKEGLDKIGIKDQSFTSGPNKALGSPTTEMTAEQRKILQSIIDSLYSRFFEVVKKGRPKVSEARLKEICD
GRIFTAEQAQKEGMIDFIGYFDNFVIELMKHPKYEGNPQGSPRIVTYQRGKVPVENIYQATDVKGNPISFGIADKLLGTG
TNAKFLYLWDI

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=229, Percent_Identity=31.0043668122271, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787527, Length=174, Percent_Identity=27.0114942528736, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 36431; Mature: 36299

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRKFGLIRSVVLLSVLFTESCVIGNSMNLFPPTAKSEFEEKLIAGKDQEKIVIISIEG
CCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEC
MISDEGKEAFFGPSSDSMVVRVKESLKRAERDPDVKGVILKINSPGGTVTASDIIYQEIK
EECCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEEEECCCCCEEHHHHHHHHHH
KFKERKGIPVFAGFMDTAASGAYYIAMATDSIGAHPTTVTGSVGVIMSGINVKEGLDKIG
HHHHHCCCEEEECHHHHCCCCEEEEEEECCCCCCCCCEECCCHHHEEECCCHHHHHHHCC
IKDQSFTSGPNKALGSPTTEMTAEQRKILQSIIDSLYSRFFEVVKKGRPKVSEARLKEIC
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DGRIFTAEQAQKEGMIDFIGYFDNFVIELMKHPKYEGNPQGSPRIVTYQRGKVPVENIYQ
CCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHH
ATDVKGNPISFGIADKLLGTGTNAKFLYLWDI
HCCCCCCCEEECHHHHHHCCCCCCEEEEEECC
>Mature Secondary Structure 
PLRKFGLIRSVVLLSVLFTESCVIGNSMNLFPPTAKSEFEEKLIAGKDQEKIVIISIEG
CCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEC
MISDEGKEAFFGPSSDSMVVRVKESLKRAERDPDVKGVILKINSPGGTVTASDIIYQEIK
EECCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEEEECCCCCEEHHHHHHHHHH
KFKERKGIPVFAGFMDTAASGAYYIAMATDSIGAHPTTVTGSVGVIMSGINVKEGLDKIG
HHHHHCCCEEEECHHHHCCCCEEEEEEECCCCCCCCCEECCCHHHEEECCCHHHHHHHCC
IKDQSFTSGPNKALGSPTTEMTAEQRKILQSIIDSLYSRFFEVVKKGRPKVSEARLKEIC
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DGRIFTAEQAQKEGMIDFIGYFDNFVIELMKHPKYEGNPQGSPRIVTYQRGKVPVENIYQ
CCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHH
ATDVKGNPISFGIADKLLGTGTNAKFLYLWDI
HCCCCCCCEEECHHHHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]