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The map label for this gene is ntrB [H]

Identifier: 183220319

GI number: 183220319

Start: 945705

End: 948026

Strand: Reverse

Name: ntrB [H]

Synonym: LEPBI_I0915

Alternate gene names: 183220319

Gene position: 948026-945705 (Counterclockwise)

Preceding gene: 183220320

Following gene: 183220314

Centisome position: 26.34

GC content: 32.08

Gene sequence:

>2322_bases
ATGTTATTTCTTTTTGGAACTTCCAAAAATAAATTATTCTATTTTTTTGCTGAATCTCTGACTTTATTTAGTTCCTATTT
GATCATAAGCTCGATTATGCCAATAATCGCAAGAACCATTCGCGCAAAGTGGATCTATTCAATCATGATTGTGTTTTACA
TTATTTTTGTAACTTTAATGCTTTCAGGTATTGAATTCTTTTGGCTTTCCACACCTACTTCTATATTCAATGGCCTGGTT
TTATTATCCTTTGGATTTATTACATTCAATTTAAAAGAATATCCTACAAAGCTTCGTGTTTACTTTCTGTTAGTTTGCAC
ATTACTTGCTTTGCAAAGATTATTTTTTCCCTATTTATTTTCTTTAGAAAGATACCTCCCACTTGGTTATACACTCAGTA
CATTATTTATGTTTTTGTTTGGAGTGGCTTGTATACTCTTTAGTTTTCAAGTACAAACTAAAAAATTAAACTTATCTCTT
TTTGAATTGGAGACATTACAAAAAGCAATCAAAGACGTCAACGTTCGTCTTTTGATCATGTACAATCAGTTACCAGCAAT
CATCTATAATATTGAATTCATTCCTGAACCAAGAACATCCTACATTAGTTCTAAAATGGAAGAAATAACTGGATATGGTA
TTAATTATTTTTACGAGAACTCGGAATTCTTTCGAGACATCGTCATCCAAGAAGACCAACACAAAATAGAAGAACTTTTT
GCAGGTAAATCGCCCATCATTCTCAGAATGATTCATGCGAATGGTTCGATCATTTGGACAGAACATTACGTCAATATTTC
CAATGATATTTTAGGAATTAAAAAAAGAATCGATGTTGTTGCTCTAGATATTACAAATTCTAAAAAAACAGAAATCTCTT
TACTCCAAGAAAAAAACCTCAACTCCACTGTATTTGACAATGCCGCTAATTTAATCCTCTTAACCAATTCAAAAGGATAC
ATAGAAAACATAAATCCAGCTGCTTTACGTGTCCTTCATTTGACAAAAGAAGATGTACTCAACAAATACATTCAAAACAT
TATACTTGTAGAAGAGGACAGAGAATTTTTGAAGGAAGTTTTGGATGATATCAATGAAATCCAAAATATTGCTGAAAGTT
TAATCCTTCGCTACATTACGAAAAACAATCAAACTTTATATTTGGAGTGGCGACTCGGGATCATTCGGGATGCAAAAAAT
GAACCTTCAAAAATTATTTGGATTGGTATTGATCAAACATCTAAGCGAACTGCAGAACTTGAACTGAAAGAACTCAATAA
ATCGTTAGAAGAAAAAGTAAAAGCAAGGACATTAGAATTACAAAATAGCAACACAGAACTCAACTCCGCTCTATTTGCAT
TGAGGGAAGCACAAGAGAAACTAATTCAAAATGAAAAACTCGCATCTCTTGGGCAGTTGGTCTCAGGACTATCCCATGAA
ATCAATAATCCAATTGGTATGATCAAGTCTTCTGTTGAAACATTAATAGCAGAATGGGAAGAAGAATCTTATGATTTCCA
AAATCAAACGTTAAACGAAATTCTGAATTTGATTATAGATTCAAACGATACAGGCCTTCGAATCCTTACGGGAATTTCAA
ATAGACAAGCAAGAAAATCGTTGTCTGAAGTATTCAAACAAAATGAAATCTTATTTCCTGAGGAATTGGCAGAATTATTT
GTAGATTCAGGAATTCGAAACTTATCACCTTCCATCTTAAATAAAATAAAAACTACAATGTCAAATCAAAGTGATTTCAA
ACGATTGAGAAAATTTTTATTAATGAAACAATCTTCTGAACATATTTTATATTCGATAAGGAGATTATCTAAAATCACAT
TCACTTTAAAAAACTTTGCTGGTCTCCAATCAAATTTAGAACTAATTGATTTTTCACTAATCGATACAATCCATTCAGCA
ATTTCCTTATATAAGGAATATTTTTTAAGAGATATCAATTTGATTTTAAACCTAGATTTTGATGGAAAAATTCGCTGTAT
CCAAGGTGACCTTGTCCAACTATGGAGCCAAATGATATGGAATGGAATCCAAGCAGTTTCAACAAAAGGAACAATTTGGA
TTCGTAGTTTTAAGTCTTCTGACTCGATCGTCGTTGAAATTGAAGACTCTGGAATCGGTATCCCGTTCGAACACCATTCA
AAAATTTTTATGCCATTCTTCTCGACAAAAACTTCTGGCGATGGTTTAGGACTTGGACTTTATCTTGTGAAAGAAATCGT
AAACCGACACAACGCAAACATTAGTTTTGAATCTGCTCCTGGTAGAACCATATTCAGAGTTAGTTTCCCAATTACAATGT
AA

Upstream 100 bases:

>100_bases
AATCGCGTTTATTCTTTTCCATTTATCTAAAAATTCAAAATCTGAAATTAGACTCAATTATACATCTTTTTTTATCGCTG
TTTTTGTTTTACGTAATTTT

Downstream 100 bases:

>100_bases
TGGAAATTTTTCCGGAGTCATTGGTTTTTCCAATAACAAAAGAATCTTCACCAAGGACATGCAGTTCATTCATAGTGGCA
TCAACTAAGCTTGAATCAAC

Product: putative two-component sensor molecule

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 773; Mature: 773

Protein sequence:

>773_residues
MLFLFGTSKNKLFYFFAESLTLFSSYLIISSIMPIIARTIRAKWIYSIMIVFYIIFVTLMLSGIEFFWLSTPTSIFNGLV
LLSFGFITFNLKEYPTKLRVYFLLVCTLLALQRLFFPYLFSLERYLPLGYTLSTLFMFLFGVACILFSFQVQTKKLNLSL
FELETLQKAIKDVNVRLLIMYNQLPAIIYNIEFIPEPRTSYISSKMEEITGYGINYFYENSEFFRDIVIQEDQHKIEELF
AGKSPIILRMIHANGSIIWTEHYVNISNDILGIKKRIDVVALDITNSKKTEISLLQEKNLNSTVFDNAANLILLTNSKGY
IENINPAALRVLHLTKEDVLNKYIQNIILVEEDREFLKEVLDDINEIQNIAESLILRYITKNNQTLYLEWRLGIIRDAKN
EPSKIIWIGIDQTSKRTAELELKELNKSLEEKVKARTLELQNSNTELNSALFALREAQEKLIQNEKLASLGQLVSGLSHE
INNPIGMIKSSVETLIAEWEEESYDFQNQTLNEILNLIIDSNDTGLRILTGISNRQARKSLSEVFKQNEILFPEELAELF
VDSGIRNLSPSILNKIKTTMSNQSDFKRLRKFLLMKQSSEHILYSIRRLSKITFTLKNFAGLQSNLELIDFSLIDTIHSA
ISLYKEYFLRDINLILNLDFDGKIRCIQGDLVQLWSQMIWNGIQAVSTKGTIWIRSFKSSDSIVVEIEDSGIGIPFEHHS
KIFMPFFSTKTSGDGLGLGLYLVKEIVNRHNANISFESAPGRTIFRVSFPITM

Sequences:

>Translated_773_residues
MLFLFGTSKNKLFYFFAESLTLFSSYLIISSIMPIIARTIRAKWIYSIMIVFYIIFVTLMLSGIEFFWLSTPTSIFNGLV
LLSFGFITFNLKEYPTKLRVYFLLVCTLLALQRLFFPYLFSLERYLPLGYTLSTLFMFLFGVACILFSFQVQTKKLNLSL
FELETLQKAIKDVNVRLLIMYNQLPAIIYNIEFIPEPRTSYISSKMEEITGYGINYFYENSEFFRDIVIQEDQHKIEELF
AGKSPIILRMIHANGSIIWTEHYVNISNDILGIKKRIDVVALDITNSKKTEISLLQEKNLNSTVFDNAANLILLTNSKGY
IENINPAALRVLHLTKEDVLNKYIQNIILVEEDREFLKEVLDDINEIQNIAESLILRYITKNNQTLYLEWRLGIIRDAKN
EPSKIIWIGIDQTSKRTAELELKELNKSLEEKVKARTLELQNSNTELNSALFALREAQEKLIQNEKLASLGQLVSGLSHE
INNPIGMIKSSVETLIAEWEEESYDFQNQTLNEILNLIIDSNDTGLRILTGISNRQARKSLSEVFKQNEILFPEELAELF
VDSGIRNLSPSILNKIKTTMSNQSDFKRLRKFLLMKQSSEHILYSIRRLSKITFTLKNFAGLQSNLELIDFSLIDTIHSA
ISLYKEYFLRDINLILNLDFDGKIRCIQGDLVQLWSQMIWNGIQAVSTKGTIWIRSFKSSDSIVVEIEDSGIGIPFEHHS
KIFMPFFSTKTSGDGLGLGLYLVKEIVNRHNANISFESAPGRTIFRVSFPITM
>Mature_773_residues
MLFLFGTSKNKLFYFFAESLTLFSSYLIISSIMPIIARTIRAKWIYSIMIVFYIIFVTLMLSGIEFFWLSTPTSIFNGLV
LLSFGFITFNLKEYPTKLRVYFLLVCTLLALQRLFFPYLFSLERYLPLGYTLSTLFMFLFGVACILFSFQVQTKKLNLSL
FELETLQKAIKDVNVRLLIMYNQLPAIIYNIEFIPEPRTSYISSKMEEITGYGINYFYENSEFFRDIVIQEDQHKIEELF
AGKSPIILRMIHANGSIIWTEHYVNISNDILGIKKRIDVVALDITNSKKTEISLLQEKNLNSTVFDNAANLILLTNSKGY
IENINPAALRVLHLTKEDVLNKYIQNIILVEEDREFLKEVLDDINEIQNIAESLILRYITKNNQTLYLEWRLGIIRDAKN
EPSKIIWIGIDQTSKRTAELELKELNKSLEEKVKARTLELQNSNTELNSALFALREAQEKLIQNEKLASLGQLVSGLSHE
INNPIGMIKSSVETLIAEWEEESYDFQNQTLNEILNLIIDSNDTGLRILTGISNRQARKSLSEVFKQNEILFPEELAELF
VDSGIRNLSPSILNKIKTTMSNQSDFKRLRKFLLMKQSSEHILYSIRRLSKITFTLKNFAGLQSNLELIDFSLIDTIHSA
ISLYKEYFLRDINLILNLDFDGKIRCIQGDLVQLWSQMIWNGIQAVSTKGTIWIRSFKSSDSIVVEIEDSGIGIPFEHHS
KIFMPFFSTKTSGDGLGLGLYLVKEIVNRHNANISFESAPGRTIFRVSFPITM

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=118, Percent_Identity=38.9830508474576, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 89077; Mature: 89077

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLFGTSKNKLFYFFAESLTLFSSYLIISSIMPIIARTIRAKWIYSIMIVFYIIFVTLM
CEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGIEFFWLSTPTSIFNGLVLLSFGFITFNLKEYPTKLRVYFLLVCTLLALQRLFFPYLF
HCCCCEEEECCCHHHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLERYLPLGYTLSTLFMFLFGVACILFSFQVQTKKLNLSLFELETLQKAIKDVNVRLLIM
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECEEHHHHHHHHHHHHCCCEEEEEE
YNQLPAIIYNIEFIPEPRTSYISSKMEEITGYGINYFYENSEFFRDIVIQEDQHKIEELF
ECCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHH
AGKSPIILRMIHANGSIIWTEHYVNISNDILGIKKRIDVVALDITNSKKTEISLLQEKNL
CCCCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHCCEEEEEEECCCCCHHHHHHHHCCC
NSTVFDNAANLILLTNSKGYIENINPAALRVLHLTKEDVLNKYIQNIILVEEDREFLKEV
CCHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHH
LDDINEIQNIAESLILRYITKNNQTLYLEWRLGIIRDAKNEPSKIIWIGIDQTSKRTAEL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHCCCCCCCCEEEEEEECCCCCHHHHH
ELKELNKSLEEKVKARTLELQNSNTELNSALFALREAQEKLIQNEKLASLGQLVSGLSHE
HHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INNPIGMIKSSVETLIAEWEEESYDFQNQTLNEILNLIIDSNDTGLRILTGISNRQARKS
HCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHH
LSEVFKQNEILFPEELAELFVDSGIRNLSPSILNKIKTTMSNQSDFKRLRKFLLMKQSSE
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCH
HILYSIRRLSKITFTLKNFAGLQSNLELIDFSLIDTIHSAISLYKEYFLRDINLILNLDF
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECC
DGKIRCIQGDLVQLWSQMIWNGIQAVSTKGTIWIRSFKSSDSIVVEIEDSGIGIPFEHHS
CCEEEEECCHHHHHHHHHHHCCHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCC
KIFMPFFSTKTSGDGLGLGLYLVKEIVNRHNANISFESAPGRTIFRVSFPITM
EEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCC
>Mature Secondary Structure
MLFLFGTSKNKLFYFFAESLTLFSSYLIISSIMPIIARTIRAKWIYSIMIVFYIIFVTLM
CEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGIEFFWLSTPTSIFNGLVLLSFGFITFNLKEYPTKLRVYFLLVCTLLALQRLFFPYLF
HCCCCEEEECCCHHHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLERYLPLGYTLSTLFMFLFGVACILFSFQVQTKKLNLSLFELETLQKAIKDVNVRLLIM
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECEEHHHHHHHHHHHHCCCEEEEEE
YNQLPAIIYNIEFIPEPRTSYISSKMEEITGYGINYFYENSEFFRDIVIQEDQHKIEELF
ECCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHH
AGKSPIILRMIHANGSIIWTEHYVNISNDILGIKKRIDVVALDITNSKKTEISLLQEKNL
CCCCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHCCEEEEEEECCCCCHHHHHHHHCCC
NSTVFDNAANLILLTNSKGYIENINPAALRVLHLTKEDVLNKYIQNIILVEEDREFLKEV
CCHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHH
LDDINEIQNIAESLILRYITKNNQTLYLEWRLGIIRDAKNEPSKIIWIGIDQTSKRTAEL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHCCCCCCCCEEEEEEECCCCCHHHHH
ELKELNKSLEEKVKARTLELQNSNTELNSALFALREAQEKLIQNEKLASLGQLVSGLSHE
HHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INNPIGMIKSSVETLIAEWEEESYDFQNQTLNEILNLIIDSNDTGLRILTGISNRQARKS
HCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHH
LSEVFKQNEILFPEELAELFVDSGIRNLSPSILNKIKTTMSNQSDFKRLRKFLLMKQSSE
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCH
HILYSIRRLSKITFTLKNFAGLQSNLELIDFSLIDTIHSAISLYKEYFLRDINLILNLDF
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECC
DGKIRCIQGDLVQLWSQMIWNGIQAVSTKGTIWIRSFKSSDSIVVEIEDSGIGIPFEHHS
CCEEEEECCHHHHHHHHHHHCCHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCC
KIFMPFFSTKTSGDGLGLGLYLVKEIVNRHNANISFESAPGRTIFRVSFPITM
EEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]