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The map label for this gene is folC [H]

Identifier: 183220243

GI number: 183220243

Start: 849470

End: 850684

Strand: Reverse

Name: folC [H]

Synonym: LEPBI_I0836

Alternate gene names: 183220243

Gene position: 850684-849470 (Counterclockwise)

Preceding gene: 183220244

Following gene: 183220242

Centisome position: 23.63

GC content: 40.99

Gene sequence:

>1215_bases
ATGAACTTTTTAGATTTTTTAAACGAGAGGCAAAACATTGAAAAAACAAGAAACTTCAATGTATTCCAAACCTATACTCT
CGATGGTTTGAAACCTATCTTTGAACATGTAAAAAAAATAACCCAAAAGAACGTTCACAATCCATTACGCATCAGTGTTG
TGGGAACCAATGGGAAAGGTTCCACCTCACACTACTTAGCTTGTCTTTTCTCAAAATTGGGATACAAGGTAGGGCTTTAC
ACATCACCACATTTAATTTCCCCACTCGAAAGGTTCCAATTGGTTTGGGATGGAAATCCCACTCTTCCCCTTTTGGATGA
GTTGAACCTCCTTTTCCAAAACCATTTCCTACCCGAAATAGAACTTTATGAATCTTTATCCTATTTTGAATTTTTAACTG
TTTTTGCTTTCCTGTTCTTTTCTGAACAAAACACAGAAGTTCAAATTTGGGAAGCAGGCCTCGGGGGAAGGCTTGATGCT
ACCAAATTGGTAGATGCAAATCATGTGGTGCTGACAAAAATTGGCCTCGACCATTCGGCCATTTTAGGGAATACAAAAGA
AGCCATCTGCCAAGAAAAGTTAGCCATCATTGGAGATCGTTGTTTGTCATTATTTGCGATGGAAGAAGAAGTGGAATCCT
TAAAGACATCATTTGTTAATCGACCAAGAGCCAATTCCGTTCCCTTACATATATTTTCTTGTCCACCAAATGAAAGTTAT
CTGGAAACTAATTTCCTTTTTGCAAAAGATACCATCTCCCAAATCATTCCCAATATACAAAAAAACCTCGCCTCCCTTTC
CTTCCAGGACCTTCCCAAACCCAGAGGAAGGATGGAGGTTATGCAAAGGGAGCCACTCGTAGTCTTTGACCCTTCCCATA
ACCCTGATGCCATTTTGACCACGACTCAGGAATTTGCAAAATCCCAATCCAAATTCCACATCTTCCTCGGAAGCCTTCCC
GACAAAGACCTAAAAGGCATCCTCGAAGCCCTTTCTCCATCGGAAACGAAGATCGAAAGCCTCCTCCTTTGGGAGGGAGA
GGGGTTTGGATCGTTTCCAGAACCAACGGGTGCCTTGTCTGCGAAGACAGATAGGGTCCGAACAACTGCGGAAATCCTAC
CCTTTTTCCAAGGCAGAATACCGATTTTGGTGTTAGGAAGTTTCCGACTTTATGGAATTGTGGCAAATTTAATACAAAAT
ACAATAAAGATGTAA

Upstream 100 bases:

>100_bases
AAAGTCAAACCAAAATGGATCAAAAAAGTAGAGTCCATTCTTTTACATGAATTGAAAAAAAGAAAATAAAATCTGTATGA
AGGTAAACTTTTACTAAATC

Downstream 100 bases:

>100_bases
AATTGGACTTTACAAATGAATCCTGAACGAGATTGTTCTTTTAGGAAACGTCGTTCAGGACTATGCAAACTCCAAAAGAA
CATACCAGAAAAGAAATTCT

Product: bifunctional folylpolyglutamate synthase/tetrahydrofolate ynthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MNFLDFLNERQNIEKTRNFNVFQTYTLDGLKPIFEHVKKITQKNVHNPLRISVVGTNGKGSTSHYLACLFSKLGYKVGLY
TSPHLISPLERFQLVWDGNPTLPLLDELNLLFQNHFLPEIELYESLSYFEFLTVFAFLFFSEQNTEVQIWEAGLGGRLDA
TKLVDANHVVLTKIGLDHSAILGNTKEAICQEKLAIIGDRCLSLFAMEEEVESLKTSFVNRPRANSVPLHIFSCPPNESY
LETNFLFAKDTISQIIPNIQKNLASLSFQDLPKPRGRMEVMQREPLVVFDPSHNPDAILTTTQEFAKSQSKFHIFLGSLP
DKDLKGILEALSPSETKIESLLLWEGEGFGSFPEPTGALSAKTDRVRTTAEILPFFQGRIPILVLGSFRLYGIVANLIQN
TIKM

Sequences:

>Translated_404_residues
MNFLDFLNERQNIEKTRNFNVFQTYTLDGLKPIFEHVKKITQKNVHNPLRISVVGTNGKGSTSHYLACLFSKLGYKVGLY
TSPHLISPLERFQLVWDGNPTLPLLDELNLLFQNHFLPEIELYESLSYFEFLTVFAFLFFSEQNTEVQIWEAGLGGRLDA
TKLVDANHVVLTKIGLDHSAILGNTKEAICQEKLAIIGDRCLSLFAMEEEVESLKTSFVNRPRANSVPLHIFSCPPNESY
LETNFLFAKDTISQIIPNIQKNLASLSFQDLPKPRGRMEVMQREPLVVFDPSHNPDAILTTTQEFAKSQSKFHIFLGSLP
DKDLKGILEALSPSETKIESLLLWEGEGFGSFPEPTGALSAKTDRVRTTAEILPFFQGRIPILVLGSFRLYGIVANLIQN
TIKM
>Mature_404_residues
MNFLDFLNERQNIEKTRNFNVFQTYTLDGLKPIFEHVKKITQKNVHNPLRISVVGTNGKGSTSHYLACLFSKLGYKVGLY
TSPHLISPLERFQLVWDGNPTLPLLDELNLLFQNHFLPEIELYESLSYFEFLTVFAFLFFSEQNTEVQIWEAGLGGRLDA
TKLVDANHVVLTKIGLDHSAILGNTKEAICQEKLAIIGDRCLSLFAMEEEVESLKTSFVNRPRANSVPLHIFSCPPNESY
LETNFLFAKDTISQIIPNIQKNLASLSFQDLPKPRGRMEVMQREPLVVFDPSHNPDAILTTTQEFAKSQSKFHIFLGSLP
DKDLKGILEALSPSETKIESLLLWEGEGFGSFPEPTGALSAKTDRVRTTAEILPFFQGRIPILVLGSFRLYGIVANLIQN
TIKM

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=189, Percent_Identity=31.2169312169312, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI66932990, Length=189, Percent_Identity=31.2169312169312, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1788654, Length=319, Percent_Identity=29.153605015674, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=205, Percent_Identity=32.1951219512195, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17553148, Length=205, Percent_Identity=32.1951219512195, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71984923, Length=205, Percent_Identity=32.1951219512195, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323760, Length=170, Percent_Identity=38.2352941176471, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324815, Length=303, Percent_Identity=25.0825082508251, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24641571, Length=230, Percent_Identity=30, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24581568, Length=160, Percent_Identity=31.875, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45613; Mature: 45613

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLDFLNERQNIEKTRNFNVFQTYTLDGLKPIFEHVKKITQKNVHNPLRISVVGTNGKG
CCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
STSHYLACLFSKLGYKVGLYTSPHLISPLERFQLVWDGNPTLPLLDELNLLFQNHFLPEI
CHHHHHHHHHHHHCCEEECCCCCHHHCHHHHEEEEECCCCCCCHHHHHHHHHHHCCCCHH
ELYESLSYFEFLTVFAFLFFSEQNTEVQIWEAGLGGRLDATKLVDANHVVLTKIGLDHSA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCEEEEEECCCCHH
ILGNTKEAICQEKLAIIGDRCLSLFAMEEEVESLKTSFVNRPRANSVPLHIFSCPPNESY
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCE
LETNFLFAKDTISQIIPNIQKNLASLSFQDLPKPRGRMEVMQREPLVVFDPSHNPDAILT
EEEEEEEHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHCCCEEEECCCCCCCEEEE
TTQEFAKSQSKFHIFLGSLPDKDLKGILEALSPSETKIESLLLWEGEGFGSFPEPTGALS
EHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHEEEEECCCCCCCCCCCCCCCC
AKTDRVRTTAEILPFFQGRIPILVLGSFRLYGIVANLIQNTIKM
CCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFLDFLNERQNIEKTRNFNVFQTYTLDGLKPIFEHVKKITQKNVHNPLRISVVGTNGKG
CCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
STSHYLACLFSKLGYKVGLYTSPHLISPLERFQLVWDGNPTLPLLDELNLLFQNHFLPEI
CHHHHHHHHHHHHCCEEECCCCCHHHCHHHHEEEEECCCCCCCHHHHHHHHHHHCCCCHH
ELYESLSYFEFLTVFAFLFFSEQNTEVQIWEAGLGGRLDATKLVDANHVVLTKIGLDHSA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCEEEEEECCCCHH
ILGNTKEAICQEKLAIIGDRCLSLFAMEEEVESLKTSFVNRPRANSVPLHIFSCPPNESY
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCE
LETNFLFAKDTISQIIPNIQKNLASLSFQDLPKPRGRMEVMQREPLVVFDPSHNPDAILT
EEEEEEEHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHCCCEEEECCCCCCCEEEE
TTQEFAKSQSKFHIFLGSLPDKDLKGILEALSPSETKIESLLLWEGEGFGSFPEPTGALS
EHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHEEEEECCCCCCCCCCCCCCCC
AKTDRVRTTAEILPFFQGRIPILVLGSFRLYGIVANLIQNTIKM
CCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]