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The map label for this gene is radA [H]

Identifier: 183220184

GI number: 183220184

Start: 791305

End: 792672

Strand: Direct

Name: radA [H]

Synonym: LEPBI_I0774

Alternate gene names: 183220184

Gene position: 791305-792672 (Clockwise)

Preceding gene: 183220183

Following gene: 183220185

Centisome position: 21.98

GC content: 45.1

Gene sequence:

>1368_bases
ATGGCAAAAAAACAACTCCCCCAATACCAATGCAAAGCTTGTGGCGATACCTTTAGCCGTTGGGCAGGGAAATGCCCATC
TTGTGGCGAATGGAACCAAATTGAGGAAATGGCAAACACTTCCCAAGGGAGGTTTGATACACCTATATCTCTAAAACCGA
GAGACAGAAGGTATACCGAACCAAAATCAATTGGTTCGGTTGTGAGTGATTCCCATACAAGGACACAAACAGGATTTAGT
GAATTGGATTTGGTACTGGGTGGAGGTATTGTCCCCGGAAGTTTGGTGTTAGTTGGTGGAGAACCAGGAGTTGGAAAATC
AACTTTGGTTTTGGAAATAGCAAAAAACATCGCAAACCAAGGCACAGTTTTGTACATCTCTGGTGAAGAATCGGCTTCCC
AAATTGGTCTTCGTGCCAAACGTATGGGTGTCACCTCTGAAAATATATTACTCTCATCCGAAGTGTATGCGGAAAACATT
GCGCAGATGATTTCCGATATAAAACCAAAAGTTGTTTTTATCGATTCCATCCAAACCATTATCAAAGAAAGCCTTGTGAA
CCAAGCAGGGACCATCACCCAACTTCGTGAGTCGTCCCAAGTGTTTTTAGAAACCGCCAAACGTACGTCAGTTCCTATTT
TTCTCATAGGCCACATCACAAAAGACGGCCAAATTGCAGGCCCCAAAGTATTGGAACATTTGGTGGATACCGTTTTGTAC
TTTGAAGGAGACAGATTCAATTATTACCGCATTTTACGTGCTGTGAAAAATCGATTTGGAGCCGTAGGAGACACTGCAAT
TTTTGAAATGGTCCTCGGTGGACTGAGTCAAGTCCTAGACCGCCATCGTTTGTTCATTTCACCCGAAACAGAAGAACGAT
CTGGGAGTGTCCTGTCTTCTGTGATGGAAGGGTCCCGTGCCATCAGTGTGGAAGTGCAGGCCCTTGTCACAAAGTCTTCT
TATGGACAGGCGCGCCGTATGGCAGAAGGACTCGATAACCGACGTGTGATTTTACTTTCCGCTGTCATCGAAAAATACTT
AGGCCTTCCTCTTTCGGAATCTGACATCTTCAGCAATTTGGCAGGCGGTCTCAGCGTGGACGAACCAAGCCTTGATCTTG
CGATTGTTGCCTCGATTGTCTCCTCCTTTCGTGACAAACCAGTCTCCAGAGAGACAGGGTATTTAGGCGAAGTAGGACTT
TCAGGTGAAGTGAGAAGTGTGGGGCAAATGAGTCTTCGCATCAAAGAACTCGTTGGGATTGGGATCAGTCAAATTTACAT
CCCACATGGAAACTGGAAAGAAGTAGAAGGTATGTTCCCTTCCATCGAACTTGTCCCCATCAAACATTTACAAGCGTTAG
GTTTGTGA

Upstream 100 bases:

>100_bases
TCCGTGAAAATCGATTCACGATTGCCGAAAAGTGTTTTGGCTTTTTGGAAACCCAAGTGGAACTCGATTTATTGGAAGTG
TATAACCTTTTCGAACACTG

Downstream 100 bases:

>100_bases
GTGTCAGCGTTACCTTCTAGCCCCAAAGGATCTACCGTTCCATTGGATCACTCTCATTCGCATTGTGACACAACTTGTTA
CAGTTTATTAGTAGACGACA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MAKKQLPQYQCKACGDTFSRWAGKCPSCGEWNQIEEMANTSQGRFDTPISLKPRDRRYTEPKSIGSVVSDSHTRTQTGFS
ELDLVLGGGIVPGSLVLVGGEPGVGKSTLVLEIAKNIANQGTVLYISGEESASQIGLRAKRMGVTSENILLSSEVYAENI
AQMISDIKPKVVFIDSIQTIIKESLVNQAGTITQLRESSQVFLETAKRTSVPIFLIGHITKDGQIAGPKVLEHLVDTVLY
FEGDRFNYYRILRAVKNRFGAVGDTAIFEMVLGGLSQVLDRHRLFISPETEERSGSVLSSVMEGSRAISVEVQALVTKSS
YGQARRMAEGLDNRRVILLSAVIEKYLGLPLSESDIFSNLAGGLSVDEPSLDLAIVASIVSSFRDKPVSRETGYLGEVGL
SGEVRSVGQMSLRIKELVGIGISQIYIPHGNWKEVEGMFPSIELVPIKHLQALGL

Sequences:

>Translated_455_residues
MAKKQLPQYQCKACGDTFSRWAGKCPSCGEWNQIEEMANTSQGRFDTPISLKPRDRRYTEPKSIGSVVSDSHTRTQTGFS
ELDLVLGGGIVPGSLVLVGGEPGVGKSTLVLEIAKNIANQGTVLYISGEESASQIGLRAKRMGVTSENILLSSEVYAENI
AQMISDIKPKVVFIDSIQTIIKESLVNQAGTITQLRESSQVFLETAKRTSVPIFLIGHITKDGQIAGPKVLEHLVDTVLY
FEGDRFNYYRILRAVKNRFGAVGDTAIFEMVLGGLSQVLDRHRLFISPETEERSGSVLSSVMEGSRAISVEVQALVTKSS
YGQARRMAEGLDNRRVILLSAVIEKYLGLPLSESDIFSNLAGGLSVDEPSLDLAIVASIVSSFRDKPVSRETGYLGEVGL
SGEVRSVGQMSLRIKELVGIGISQIYIPHGNWKEVEGMFPSIELVPIKHLQALGL
>Mature_454_residues
AKKQLPQYQCKACGDTFSRWAGKCPSCGEWNQIEEMANTSQGRFDTPISLKPRDRRYTEPKSIGSVVSDSHTRTQTGFSE
LDLVLGGGIVPGSLVLVGGEPGVGKSTLVLEIAKNIANQGTVLYISGEESASQIGLRAKRMGVTSENILLSSEVYAENIA
QMISDIKPKVVFIDSIQTIIKESLVNQAGTITQLRESSQVFLETAKRTSVPIFLIGHITKDGQIAGPKVLEHLVDTVLYF
EGDRFNYYRILRAVKNRFGAVGDTAIFEMVLGGLSQVLDRHRLFISPETEERSGSVLSSVMEGSRAISVEVQALVTKSSY
GQARRMAEGLDNRRVILLSAVIEKYLGLPLSESDIFSNLAGGLSVDEPSLDLAIVASIVSSFRDKPVSRETGYLGEVGLS
GEVRSVGQMSLRIKELVGIGISQIYIPHGNWKEVEGMFPSIELVPIKHLQALGL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=442, Percent_Identity=38.9140271493213, Blast_Score=323, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49493; Mature: 49362

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKQLPQYQCKACGDTFSRWAGKCPSCGEWNQIEEMANTSQGRFDTPISLKPRDRRYTE
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PKSIGSVVSDSHTRTQTGFSELDLVLGGGIVPGSLVLVGGEPGVGKSTLVLEIAKNIANQ
HHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCC
GTVLYISGEESASQIGLRAKRMGVTSENILLSSEVYAENIAQMISDIKPKVVFIDSIQTI
CEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEHHHHHHH
IKESLVNQAGTITQLRESSQVFLETAKRTSVPIFLIGHITKDGQIAGPKVLEHLVDTVLY
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
FEGDRFNYYRILRAVKNRFGAVGDTAIFEMVLGGLSQVLDRHRLFISPETEERSGSVLSS
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHH
VMEGSRAISVEVQALVTKSSYGQARRMAEGLDNRRVILLSAVIEKYLGLPLSESDIFSNL
HHCCCCEEEEEEEEEEECHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHH
AGGLSVDEPSLDLAIVASIVSSFRDKPVSRETGYLGEVGLSGEVRSVGQMSLRIKELVGI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
GISQIYIPHGNWKEVEGMFPSIELVPIKHLQALGL
CCEEEEECCCCCHHHCCCCCCEEEEEHHHHHHHCC
>Mature Secondary Structure 
AKKQLPQYQCKACGDTFSRWAGKCPSCGEWNQIEEMANTSQGRFDTPISLKPRDRRYTE
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PKSIGSVVSDSHTRTQTGFSELDLVLGGGIVPGSLVLVGGEPGVGKSTLVLEIAKNIANQ
HHHHHHHHCCCCCHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCC
GTVLYISGEESASQIGLRAKRMGVTSENILLSSEVYAENIAQMISDIKPKVVFIDSIQTI
CEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEHHHHHHH
IKESLVNQAGTITQLRESSQVFLETAKRTSVPIFLIGHITKDGQIAGPKVLEHLVDTVLY
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
FEGDRFNYYRILRAVKNRFGAVGDTAIFEMVLGGLSQVLDRHRLFISPETEERSGSVLSS
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHH
VMEGSRAISVEVQALVTKSSYGQARRMAEGLDNRRVILLSAVIEKYLGLPLSESDIFSNL
HHCCCCEEEEEEEEEEECHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHH
AGGLSVDEPSLDLAIVASIVSSFRDKPVSRETGYLGEVGLSGEVRSVGQMSLRIKELVGI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
GISQIYIPHGNWKEVEGMFPSIELVPIKHLQALGL
CCEEEEECCCCCHHHCCCCCCEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]