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The map label for this gene is cobB [H]

Identifier: 183220133

GI number: 183220133

Start: 735265

End: 736020

Strand: Reverse

Name: cobB [H]

Synonym: LEPBI_I0721

Alternate gene names: 183220133

Gene position: 736020-735265 (Counterclockwise)

Preceding gene: 183220135

Following gene: 183220132

Centisome position: 20.45

GC content: 41.67

Gene sequence:

>756_bases
ATGAACCTTTTGCCCCAAGATTCTTTGGAACTCATACAAAACGCTCCTTCCATCGTTTTTTTAACAGGTGCTGGCATTTC
GAGCGAAAGTGGGATTCCTACCTTTCGCGGAGAAGGTGGCCTTTGGAAAAACTTCCGAGCAGAGGATCTGGCAACACCCG
AAGCCTTTCAAAAGAATCCAAAACTTGTATGGGAATGGTATGATTGGAGAAGGGAAATTTGTCAAAAAGCAAAACCAAAC
CTCGGCCACCTAATCATCGCAAAATGGCAAAAAAAATCTAGTTCCGTTCATCTCATCACACAAAATGTAGATGGACTCCA
CCCTCGTGCCGGAAGTGAAACTCTGATCGAATTACATGGGAATATCTTTCGAGTGAGGTGTACAAATTGTACGTCAATAT
TCCATTTGGAAAAAGATGGATTGGATCATCCAGGACTCAAATTTTGTAAAGAATGTGATTCCCTACTCCGCCCTGATATT
GTCTGGTTTGGTGAAGAATACAACCAAAATTTACTCACGAAGAGTTGGGAACTTTGTAAACAATCGCAAGTGGTGATTTT
GATCGGAACAAGTGCCAATGTATCGGTTCCAACCAATTTGGCCCAAACCGCCATTCGCAATGGTGCCATCGGGATTGAAA
TCAATCCAGAGGAAACAAATCTAACACCTTCCATGAAACATCACTTTGATGGGAAATCGGGAGAAGTATTACCTCAAATT
TGGAAGGAAGTATTTCCAGATGAAACTATGGAATAA

Upstream 100 bases:

>100_bases
TGAATTTCTTGCCAATGACGGTTCACAACGACCAGAATTCCTAGACTGAAATTTTTTCCGATTTAAATTTTGAAAGAATT
TGTTAGAATGAACCAAAGAA

Downstream 100 bases:

>100_bases
GATTCCTTGTAATCTCAAAAGAGTTAGAGTTAAATTTTAGCTCTAACCGATTAGGAATGAAGTCATGAAATTTTACAGAA
TGTAAATCTGATATTCTTTG

Product: putative NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNLLPQDSLELIQNAPSIVFLTGAGISSESGIPTFRGEGGLWKNFRAEDLATPEAFQKNPKLVWEWYDWRREICQKAKPN
LGHLIIAKWQKKSSSVHLITQNVDGLHPRAGSETLIELHGNIFRVRCTNCTSIFHLEKDGLDHPGLKFCKECDSLLRPDI
VWFGEEYNQNLLTKSWELCKQSQVVILIGTSANVSVPTNLAQTAIRNGAIGIEINPEETNLTPSMKHHFDGKSGEVLPQI
WKEVFPDETME

Sequences:

>Translated_251_residues
MNLLPQDSLELIQNAPSIVFLTGAGISSESGIPTFRGEGGLWKNFRAEDLATPEAFQKNPKLVWEWYDWRREICQKAKPN
LGHLIIAKWQKKSSSVHLITQNVDGLHPRAGSETLIELHGNIFRVRCTNCTSIFHLEKDGLDHPGLKFCKECDSLLRPDI
VWFGEEYNQNLLTKSWELCKQSQVVILIGTSANVSVPTNLAQTAIRNGAIGIEINPEETNLTPSMKHHFDGKSGEVLPQI
WKEVFPDETME
>Mature_251_residues
MNLLPQDSLELIQNAPSIVFLTGAGISSESGIPTFRGEGGLWKNFRAEDLATPEAFQKNPKLVWEWYDWRREICQKAKPN
LGHLIIAKWQKKSSSVHLITQNVDGLHPRAGSETLIELHGNIFRVRCTNCTSIFHLEKDGLDHPGLKFCKECDSLLRPDI
VWFGEEYNQNLLTKSWELCKQSQVVILIGTSANVSVPTNLAQTAIRNGAIGIEINPEETNLTPSMKHHFDGKSGEVLPQI
WKEVFPDETME

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=241, Percent_Identity=39.8340248962656, Blast_Score=180, Evalue=9e-46,
Organism=Homo sapiens, GI6912664, Length=258, Percent_Identity=37.5968992248062, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI13787215, Length=246, Percent_Identity=37.3983739837398, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI6912660, Length=224, Percent_Identity=33.0357142857143, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI63054862, Length=207, Percent_Identity=32.8502415458937, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI6912662, Length=268, Percent_Identity=27.9850746268657, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI7657575, Length=241, Percent_Identity=28.2157676348548, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI300797577, Length=249, Percent_Identity=27.710843373494, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI7706712, Length=202, Percent_Identity=29.2079207920792, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI13775602, Length=206, Percent_Identity=30.0970873786408, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI13775600, Length=206, Percent_Identity=30.0970873786408, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI300797705, Length=193, Percent_Identity=30.5699481865285, Blast_Score=78, Evalue=6e-15,
Organism=Escherichia coli, GI308199517, Length=234, Percent_Identity=35.8974358974359, Blast_Score=146, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17541892, Length=222, Percent_Identity=28.8288288288288, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17567771, Length=270, Percent_Identity=29.2592592592593, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990482, Length=271, Percent_Identity=28.4132841328413, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71990487, Length=273, Percent_Identity=28.2051282051282, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17505510, Length=219, Percent_Identity=30.5936073059361, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325242, Length=221, Percent_Identity=31.2217194570136, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28571445, Length=191, Percent_Identity=29.3193717277487, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17137536, Length=248, Percent_Identity=27.0161290322581, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648389, Length=189, Percent_Identity=30.1587301587302, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24650933, Length=202, Percent_Identity=27.2277227722772, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24645650, Length=131, Percent_Identity=33.587786259542, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 28273; Mature: 28273

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLPQDSLELIQNAPSIVFLTGAGISSESGIPTFRGEGGLWKNFRAEDLATPEAFQKNP
CCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEECCCCCCCCCCCCHHCCCCHHHHCCC
KLVWEWYDWRREICQKAKPNLGHLIIAKWQKKSSSVHLITQNVDGLHPRAGSETLIELHG
CEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEECC
NIFRVRCTNCTSIFHLEKDGLDHPGLKFCKECDSLLRPDIVWFGEEYNQNLLTKSWELCK
CEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCHHCCHHHHHHHHHHC
QSQVVILIGTSANVSVPTNLAQTAIRNGAIGIEINPEETNLTPSMKHHFDGKSGEVLPQI
CCCEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHCCCCCCCHHHHH
WKEVFPDETME
HHHHCCCCCCC
>Mature Secondary Structure
MNLLPQDSLELIQNAPSIVFLTGAGISSESGIPTFRGEGGLWKNFRAEDLATPEAFQKNP
CCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEECCCCCCCCCCCCHHCCCCHHHHCCC
KLVWEWYDWRREICQKAKPNLGHLIIAKWQKKSSSVHLITQNVDGLHPRAGSETLIELHG
CEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEECC
NIFRVRCTNCTSIFHLEKDGLDHPGLKFCKECDSLLRPDIVWFGEEYNQNLLTKSWELCK
CEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCHHCCHHHHHHHHHHC
QSQVVILIGTSANVSVPTNLAQTAIRNGAIGIEINPEETNLTPSMKHHFDGKSGEVLPQI
CCCEEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHCCCCCCCHHHHH
WKEVFPDETME
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA