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The map label for this gene is yajL [H]

Identifier: 183219940

GI number: 183219940

Start: 521100

End: 521666

Strand: Reverse

Name: yajL [H]

Synonym: LEPBI_I0522

Alternate gene names: 183219940

Gene position: 521666-521100 (Counterclockwise)

Preceding gene: 183219944

Following gene: 183219932

Centisome position: 14.49

GC content: 44.97

Gene sequence:

>567_bases
ATGGCCAAAAAGGTTTTGATTCCCCTCTGTCCTGGTTTTGAAGAAATGGAAGCCATCATTCTCATTGATGTCCTTCGTCG
CGGGAATGTGGAAGTGGTTTCAGCCAGCAAAACAAAAGAACCAGTCGTGGCTTCTCGGAATACAATCCATATCAGTGACA
CAACCTTTTCAGAGATCAATGTTGATGAATTTGATGCGATTGTTTTGCCCGGTGGGATGAATGGAACAAAAAATTTGATG
GCCGATACAGAAATCCAAAAGATCCTCTCTATCTTCCATTCCTCCAAAAAACACATTGGTGCTATCTGTGCCGCGCCTGC
CGTCTTACGAAAATGGGATATCATCTCTGGGAATGACCCATACACGGCCTTTCCCTCCACAGACGATTTGGCGAAAGGAA
AAGGGGGAAGGTATACGGGAAATCGGATTGAGTCCTTTCACCATATCCATACGAGTGTTGGACCTGGATCAGCATTTGCA
TTTGCTTTGTATTTGTTGGAATTGTTCGAAGGGAAGGAAGTGATGGAGAGAGTCAAACAAGGACTCCAACTGCCTCAATC
GGAGTGA

Upstream 100 bases:

>100_bases
AGTATCAGACCCAAAGAAGGGCGGGAAAGTTCCCATTTCTTGGGATCGTTTTTTTACAAAAAAACTTGAACAATTTGTAA
CACGTCAACCAAGTCTTAGT

Downstream 100 bases:

>100_bases
GAATTTCGTTCGCTGATGAGAGACTGGACTGCCATCTCTTCGGTATCAAACACTTCTAAGTGACTTTCCATCCCAGTCAT
TTTAAACACTTTGGCGACGG

Product: ThiJ/PfpI family peptidase

Products: 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyridinium; 4-Methyl-5-(2-hydroxyethyl)-thiazole

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MAKKVLIPLCPGFEEMEAIILIDVLRRGNVEVVSASKTKEPVVASRNTIHISDTTFSEINVDEFDAIVLPGGMNGTKNLM
ADTEIQKILSIFHSSKKHIGAICAAPAVLRKWDIISGNDPYTAFPSTDDLAKGKGGRYTGNRIESFHHIHTSVGPGSAFA
FALYLLELFEGKEVMERVKQGLQLPQSE

Sequences:

>Translated_188_residues
MAKKVLIPLCPGFEEMEAIILIDVLRRGNVEVVSASKTKEPVVASRNTIHISDTTFSEINVDEFDAIVLPGGMNGTKNLM
ADTEIQKILSIFHSSKKHIGAICAAPAVLRKWDIISGNDPYTAFPSTDDLAKGKGGRYTGNRIESFHHIHTSVGPGSAFA
FALYLLELFEGKEVMERVKQGLQLPQSE
>Mature_187_residues
AKKVLIPLCPGFEEMEAIILIDVLRRGNVEVVSASKTKEPVVASRNTIHISDTTFSEINVDEFDAIVLPGGMNGTKNLMA
DTEIQKILSIFHSSKKHIGAICAAPAVLRKWDIISGNDPYTAFPSTDDLAKGKGGRYTGNRIESFHHIHTSVGPGSAFAF
ALYLLELFEGKEVMERVKQGLQLPQSE

Specific function: Involved in biogenesis of ribosomal proteins, probably as a ribosomal protein-folding chaperone. Interacts with ribosomal subunits, ribosomes and polysomes. Confers resistance to oxidative stress [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C56 family [H]

Homologues:

Organism=Homo sapiens, GI183227678, Length=185, Percent_Identity=36.7567567567568, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI31543380, Length=185, Percent_Identity=36.7567567567568, Blast_Score=111, Evalue=5e-25,
Organism=Escherichia coli, GI87081736, Length=191, Percent_Identity=34.5549738219895, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17531319, Length=176, Percent_Identity=36.9318181818182, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17558714, Length=160, Percent_Identity=33.75, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571932, Length=183, Percent_Identity=36.6120218579235, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24653499, Length=183, Percent_Identity=37.7049180327869, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006287
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: 2.5.1.2

Molecular weight: Translated: 20554; Mature: 20423

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKVLIPLCPGFEEMEAIILIDVLRRGNVEVVSASKTKEPVVASRNTIHISDTTFSEIN
CCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCEEEECCCCCCCCC
VDEFDAIVLPGGMNGTKNLMADTEIQKILSIFHSSKKHIGAICAAPAVLRKWDIISGNDP
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
YTAFPSTDDLAKGKGGRYTGNRIESFHHIHTSVGPGSAFAFALYLLELFEGKEVMERVKQ
EEECCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
GLQLPQSE
HCCCCCCC
>Mature Secondary Structure 
AKKVLIPLCPGFEEMEAIILIDVLRRGNVEVVSASKTKEPVVASRNTIHISDTTFSEIN
CCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCEEEECCCCCCCCC
VDEFDAIVLPGGMNGTKNLMADTEIQKILSIFHSSKKHIGAICAAPAVLRKWDIISGNDP
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
YTAFPSTDDLAKGKGGRYTGNRIESFHHIHTSVGPGSAFAFALYLLELFEGKEVMERVKQ
EEECCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
GLQLPQSE
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: thiamine; pyridine

Specific reaction: thiamine + pyridine = 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyridinium + 4-methyl-5-(2-hydroxyethyl)thiazole

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9592144 [H]