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The map label for this gene is pdxJ

Identifier: 183219894

GI number: 183219894

Start: 472054

End: 472836

Strand: Reverse

Name: pdxJ

Synonym: LEPBI_I0476

Alternate gene names: 183219894

Gene position: 472836-472054 (Counterclockwise)

Preceding gene: 183219904

Following gene: 183219892

Centisome position: 13.14

GC content: 41.25

Gene sequence:

>783_bases
ATGACCCAATTAAGTGTCAATGTCAATAAGATCGCCACTCTCCGCAATTCTCGTGGAGGATCCCTTCCTAGTGTCCTGAA
ACTGTCAGAACTCATCTTGGATTCAGGTGCTCATGGGATCACTGTTCACCCTCGTTCGGACGAAAGGCATATCACCAAAC
AAGATGTATTCGAATTACAGGAGTTTTTACGAACTTACAACGAAAAAATTACTAAATTAGGAATTTCTAAAAAAGAGTAC
AATATAGAAGGCGAACCAAGTGAACGTTTTTTAGACCTAGTACTCAAAGCAAAACCAGACCAAGCCACTCTCGTTCCCGT
GAAACCAGGCGAAATCACCTCGGATCATGGATTTCAATTTGCCGATCAAAAAACGTTTTCGACCTTAAAACCCATTGTAG
AGGCAATTCGAAAGGAAGGGATCCGTGTTTCTTTGTTTATGGAAACCGATTTTACGTTTTATGACCAAGTGGTTGCCCTT
GGTGCCGAACGAATTGAACTCTATACAGGTCCTTTTGCACATGCTTTCGATCTTTCGGAAGAAAAAGGAAAAGAAATTTT
CACTGATTACCAAAGAGCCGCCATTGAGGCAAACAAACTAGGGTTAGCTGTGAATGCGGGACACGATTTGGATACCAATA
ATCTACGAGTGTTTGCAAAACTTCCTTACTTAAAAGAAGTTTCCATTGGGCATCGTTTGATGGCCCAAAGTTTAGTGGAT
GGTTTAGAAACGACAGTCAAATCCTATCTCAAAGTTCTTTCGCTAGGGAACGAAACTGAGTGA

Upstream 100 bases:

>100_bases
GTACTTTTGACAGTTTTGGAAATCCCCGTTACGGGACAACCTTTTTGAAATTGGACGGTTTTCTCCTGGATGAAAATTAA
TGAAAAGTTTTTTTTTTCCT

Downstream 100 bases:

>100_bases
ACTGAGGGTCTTGTTCGATGAGTAATTTGGCGGCTCGTTCTAGAGCCGTTGCCATGAATGCTTCTGTTTTGCCTTTCCGT
TTCACAATCTGCACTTCCGC

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTQLSVNVNKIATLRNSRGGSLPSVLKLSELILDSGAHGITVHPRSDERHITKQDVFELQEFLRTYNEKITKLGISKKEY
NIEGEPSERFLDLVLKAKPDQATLVPVKPGEITSDHGFQFADQKTFSTLKPIVEAIRKEGIRVSLFMETDFTFYDQVVAL
GAERIELYTGPFAHAFDLSEEKGKEIFTDYQRAAIEANKLGLAVNAGHDLDTNNLRVFAKLPYLKEVSIGHRLMAQSLVD
GLETTVKSYLKVLSLGNETE

Sequences:

>Translated_260_residues
MTQLSVNVNKIATLRNSRGGSLPSVLKLSELILDSGAHGITVHPRSDERHITKQDVFELQEFLRTYNEKITKLGISKKEY
NIEGEPSERFLDLVLKAKPDQATLVPVKPGEITSDHGFQFADQKTFSTLKPIVEAIRKEGIRVSLFMETDFTFYDQVVAL
GAERIELYTGPFAHAFDLSEEKGKEIFTDYQRAAIEANKLGLAVNAGHDLDTNNLRVFAKLPYLKEVSIGHRLMAQSLVD
GLETTVKSYLKVLSLGNETE
>Mature_259_residues
TQLSVNVNKIATLRNSRGGSLPSVLKLSELILDSGAHGITVHPRSDERHITKQDVFELQEFLRTYNEKITKLGISKKEYN
IEGEPSERFLDLVLKAKPDQATLVPVKPGEITSDHGFQFADQKTFSTLKPIVEAIRKEGIRVSLFMETDFTFYDQVVALG
AERIELYTGPFAHAFDLSEEKGKEIFTDYQRAAIEANKLGLAVNAGHDLDTNNLRVFAKLPYLKEVSIGHRLMAQSLVDG
LETTVKSYLKVLSLGNETE

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=250, Percent_Identity=33.6, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_LEPBA (B0SBH6)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961575.1
- ProteinModelPortal:   B0SBH6
- SMR:   B0SBH6
- GeneID:   6387825
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0458
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- BioCyc:   LBIF355278:LBF_0458-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 29083; Mature: 28951

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: ACT_SITE 43-43 ACT_SITE 83-83 ACT_SITE 208-208 BINDING 7-7 BINDING 18-18 BINDING 45-45 BINDING 50-50 BINDING 113-113 BINDING 209-209

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLSVNVNKIATLRNSRGGSLPSVLKLSELILDSGAHGITVHPRSDERHITKQDVFELQ
CCEEEECHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHH
EFLRTYNEKITKLGISKKEYNIEGEPSERFLDLVLKAKPDQATLVPVKPGEITSDHGFQF
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
ADQKTFSTLKPIVEAIRKEGIRVSLFMETDFTFYDQVVALGAERIELYTGPFAHAFDLSE
CCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCHHHEEEEECCCHHEECCCH
EKGKEIFTDYQRAAIEANKLGLAVNAGHDLDTNNLRVFAKLPYLKEVSIGHRLMAQSLVD
HHCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
GLETTVKSYLKVLSLGNETE
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQLSVNVNKIATLRNSRGGSLPSVLKLSELILDSGAHGITVHPRSDERHITKQDVFELQ
CEEEECHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHH
EFLRTYNEKITKLGISKKEYNIEGEPSERFLDLVLKAKPDQATLVPVKPGEITSDHGFQF
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
ADQKTFSTLKPIVEAIRKEGIRVSLFMETDFTFYDQVVALGAERIELYTGPFAHAFDLSE
CCHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCHHHEEEEECCCHHEECCCH
EKGKEIFTDYQRAAIEANKLGLAVNAGHDLDTNNLRVFAKLPYLKEVSIGHRLMAQSLVD
HHCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
GLETTVKSYLKVLSLGNETE
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA