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The map label for this gene is algI [H]

Identifier: 183219602

GI number: 183219602

Start: 171350

End: 172732

Strand: Direct

Name: algI [H]

Synonym: LEPBI_I0177

Alternate gene names: 183219602

Gene position: 171350-172732 (Clockwise)

Preceding gene: 183219601

Following gene: 183219603

Centisome position: 4.76

GC content: 30.15

Gene sequence:

>1383_bases
GTGTTATTTAACTCATTTGTATTTGTCGTATTTTTTGTATCATTATATACCATCTATTGGCAACTGAATTCTAGATTTCA
AAAACTGACCATTTTAATTGGCTCCCTAATCTTTTATGGGTATTGGGATCTTTTGTTTTTGTGCCATTTTCTCTTAATTG
TTTTACTCAATTACGCATTTTATAAGATCACAAATTATAAATTTAATAAGATTCAAATTACAGGTATTCTTGTATTTAAT
TTATTAAATTTAATCTTTTTTAAGTATTTTTACTTTTTAGTAAATATTATCAAAGATTTGTTTGGATACCAACAAATTAC
AGAAGTAACTTCTTTTTTACCGAACATAGTGTTACCTCTTGCGATTAGTTTTTATACCTTTCAAATTTTAGCATTTCAAA
TTGATGTATATAGAGGAAAAATTCAATCATCAGTATCGGCTATAGATTTTACAATTTTCATTATGTTTTTTCCTCAATTG
ATTGCCGGTCCAATCATGAGGCATACAGATTTTTTCAAACAATTACACAGCAAAAAAAGATTTTTGGAGCTTCCTTTCAA
TGCAGGTGGTTACCTCATCTTGTTGGGTGTTTTAAAAAAAGTTATTGTTGCGGATAATATTTCTCCATTAATCGAACCAT
TCTTTAGCCATCCTGAAAATTTTAATTTTTTAAGTGCTCTTTTGTCTGTTTATGGCTTCGCAATCCAAATCTATTGTGAT
TTTTCCGGATATTGTGACATTGCACGAGGTTTGGCATTATTGCTTGGATACGATATTCCCGTTAACTTTAATGCACCTTA
CTTTTCTACTTCATTAAAAGAATTTTGGCAAAGGTGGCATATCACCCTTTCACAATGGTTGCGTGATTACCTTTACATTT
CGTTAGGTGGAAATCGATTTGGAAATATTCGAACTTATTTTAATTTAGTAGTCACTATGGTGTTAGGTGGTTTGTGGCAT
GGTGCGAACTATACTTTTTTAGTATGGGGTGCATTACACGGAATGTATTTAATAGTAGAAAGAGTTTTGACAACTAAGAA
AGAAAGTACTACATTTCATTTCGTTAAGCTAGCAAAAGGAATCATTGTATTTCATTTGGTCTGTTTTGCGTGGTTATTTT
TTAGAATTGAAAGTATTCAAGATTTTCAAACTATTTATCACAATTTAATTCAGCAATCTGGAATTGATATTGCTGAAATT
CCAAAATTGTACCGATTGATCGCGATTGGTTTTTTGCTTCATACGTATGAGTATTTCCAAAGAAATGTAGCGATACAATT
CAAATATAGAAAAATGTTTTTGCCTAGTTTAGCGATCATATGCGGTATACTAGTGTTAACGTTAACTTCAAATTCATCTC
AGTTCATATACTTTCAATTCTAG

Upstream 100 bases:

>100_bases
GAAACCAGGATTCCTTGGATTGATTTTTTACCCAGATGATAAAATGTATTTACTTAGGCTAAGGTAATTCCGACTATTAA
CTAATTATTGGGATACCACA

Downstream 100 bases:

>100_bases
GAAAAACGATGAAATTAAAAAAATTCCGCATTTTATACTTATTTTTGTTATTTTTTGCCATTGATAAATTGGTTTTGATT
CCTTCTGTAAGGGCTTTTTT

Product: putative alginate O-acetyltransferase

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLFNSFVFVVFFVSLYTIYWQLNSRFQKLTILIGSLIFYGYWDLLFLCHFLLIVLLNYAFYKITNYKFNKIQITGILVFN
LLNLIFFKYFYFLVNIIKDLFGYQQITEVTSFLPNIVLPLAISFYTFQILAFQIDVYRGKIQSSVSAIDFTIFIMFFPQL
IAGPIMRHTDFFKQLHSKKRFLELPFNAGGYLILLGVLKKVIVADNISPLIEPFFSHPENFNFLSALLSVYGFAIQIYCD
FSGYCDIARGLALLLGYDIPVNFNAPYFSTSLKEFWQRWHITLSQWLRDYLYISLGGNRFGNIRTYFNLVVTMVLGGLWH
GANYTFLVWGALHGMYLIVERVLTTKKESTTFHFVKLAKGIIVFHLVCFAWLFFRIESIQDFQTIYHNLIQQSGIDIAEI
PKLYRLIAIGFLLHTYEYFQRNVAIQFKYRKMFLPSLAIICGILVLTLTSNSSQFIYFQF

Sequences:

>Translated_460_residues
MLFNSFVFVVFFVSLYTIYWQLNSRFQKLTILIGSLIFYGYWDLLFLCHFLLIVLLNYAFYKITNYKFNKIQITGILVFN
LLNLIFFKYFYFLVNIIKDLFGYQQITEVTSFLPNIVLPLAISFYTFQILAFQIDVYRGKIQSSVSAIDFTIFIMFFPQL
IAGPIMRHTDFFKQLHSKKRFLELPFNAGGYLILLGVLKKVIVADNISPLIEPFFSHPENFNFLSALLSVYGFAIQIYCD
FSGYCDIARGLALLLGYDIPVNFNAPYFSTSLKEFWQRWHITLSQWLRDYLYISLGGNRFGNIRTYFNLVVTMVLGGLWH
GANYTFLVWGALHGMYLIVERVLTTKKESTTFHFVKLAKGIIVFHLVCFAWLFFRIESIQDFQTIYHNLIQQSGIDIAEI
PKLYRLIAIGFLLHTYEYFQRNVAIQFKYRKMFLPSLAIICGILVLTLTSNSSQFIYFQF
>Mature_460_residues
MLFNSFVFVVFFVSLYTIYWQLNSRFQKLTILIGSLIFYGYWDLLFLCHFLLIVLLNYAFYKITNYKFNKIQITGILVFN
LLNLIFFKYFYFLVNIIKDLFGYQQITEVTSFLPNIVLPLAISFYTFQILAFQIDVYRGKIQSSVSAIDFTIFIMFFPQL
IAGPIMRHTDFFKQLHSKKRFLELPFNAGGYLILLGVLKKVIVADNISPLIEPFFSHPENFNFLSALLSVYGFAIQIYCD
FSGYCDIARGLALLLGYDIPVNFNAPYFSTSLKEFWQRWHITLSQWLRDYLYISLGGNRFGNIRTYFNLVVTMVLGGLWH
GANYTFLVWGALHGMYLIVERVLTTKKESTTFHFVKLAKGIIVFHLVCFAWLFFRIESIQDFQTIYHNLIQQSGIDIAEI
PKLYRLIAIGFLLHTYEYFQRNVAIQFKYRKMFLPSLAIICGILVLTLTSNSSQFIYFQF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 53962; Mature: 53962

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNSFVFVVFFVSLYTIYWQLNSRFQKLTILIGSLIFYGYWDLLFLCHFLLIVLLNYAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKITNYKFNKIQITGILVFNLLNLIFFKYFYFLVNIIKDLFGYQQITEVTSFLPNIVLPL
HHHCCCEECEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
AISFYTFQILAFQIDVYRGKIQSSVSAIDFTIFIMFFPQLIAGPIMRHTDFFKQLHSKKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FLELPFNAGGYLILLGVLKKVIVADNISPLIEPFFSHPENFNFLSALLSVYGFAIQIYCD
EEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEEC
FSGYCDIARGLALLLGYDIPVNFNAPYFSTSLKEFWQRWHITLSQWLRDYLYISLGGNRF
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
GNIRTYFNLVVTMVLGGLWHGANYTFLVWGALHGMYLIVERVLTTKKESTTFHFVKLAKG
CCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IIVFHLVCFAWLFFRIESIQDFQTIYHNLIQQSGIDIAEIPKLYRLIAIGFLLHTYEYFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RNVAIQFKYRKMFLPSLAIICGILVLTLTSNSSQFIYFQF
CCEEEEEEHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEC
>Mature Secondary Structure
MLFNSFVFVVFFVSLYTIYWQLNSRFQKLTILIGSLIFYGYWDLLFLCHFLLIVLLNYAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKITNYKFNKIQITGILVFNLLNLIFFKYFYFLVNIIKDLFGYQQITEVTSFLPNIVLPL
HHHCCCEECEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
AISFYTFQILAFQIDVYRGKIQSSVSAIDFTIFIMFFPQLIAGPIMRHTDFFKQLHSKKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FLELPFNAGGYLILLGVLKKVIVADNISPLIEPFFSHPENFNFLSALLSVYGFAIQIYCD
EEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEEEC
FSGYCDIARGLALLLGYDIPVNFNAPYFSTSLKEFWQRWHITLSQWLRDYLYISLGGNRF
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
GNIRTYFNLVVTMVLGGLWHGANYTFLVWGALHGMYLIVERVLTTKKESTTFHFVKLAKG
CCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IIVFHLVCFAWLFFRIESIQDFQTIYHNLIQQSGIDIAEIPKLYRLIAIGFLLHTYEYFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RNVAIQFKYRKMFLPSLAIICGILVLTLTSNSSQFIYFQF
CCEEEEEEHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8636017; 10984043; 12003941; 11208804; 11179370 [H]