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The map label for this gene is purF [H]

Identifier: 183219598

GI number: 183219598

Start: 168109

End: 169536

Strand: Direct

Name: purF [H]

Synonym: LEPBI_I0173

Alternate gene names: 183219598

Gene position: 168109-169536 (Clockwise)

Preceding gene: 183219591

Following gene: 183219599

Centisome position: 4.67

GC content: 46.36

Gene sequence:

>1428_bases
ATGATTCTCCAATCTGACAAACCAAAAGAAGAATGTGCCATATACGGCATCTACAATAGCAAGGAAGCTGCTAATTTTAC
CTACCTAGGTTTGTACTCAATGCAACACCGAGGCCAGGAGTCCAGTGGGATCGTATCCACCGATGGTTCCCACTTATACC
GGTATGCCAATATGGGCCTTGTGGCAAATATCTTCACCCAACCGAAGATCAAAGAGCTCATAGGGGATGCGGCCATTGGC
CACAACCGGTATTCCACAACGGGGGCGAGTTTTTTACGAAATGCGCAGCCCGTCCGAGTGGAATCTCACTTGGGACCTGT
GGCACTTGCCCATAATGGAAACCTTGTCAACTCTTGGGACATCCGAAACCGGCTGGAAAGGGATGGATCCATCTTCCAAA
CCACCATTGATTCGGAAGTGATTGTCCACCTGATGGCGAAAAGCCATAAAACAGACCTACTCGAAGCTCTCTGTGAATCT
CTTGCCCAGGTGCGAGGGGCCTATTCGTTGTTAGTATTAACTCCTAGATATCTAATCGCGGTCAGGGATCCGAATGGGTT
CCGACCACTTGTCATGGGGAAACGATCTGACGGAGCCATTGTCTTTGCATCTGAAACTTGTGCTTTTGACATTACGGATA
CAGAATATGTGAGAGATGTGGAACCGGGGGAAATGGTTGTCATCGACCATACAGGCATGCGTTCCCTCTACCCATTCCCC
AAGGCAAAACCAAGTCTTTGTATTTTTGAATATATCTATTTCGCAAGACCTGATTCCTATATTTTTGAAGAATCTGTCTA
CAAAGTGAGAAAGTCTCTCGGTCGCCAATTAGCGCGTGTTATGCCAGTTGAGGCCGATGTAGTCATACCAGTCCCAGATT
CCGCAAACATTGCCGCCCTTGGTTACAGTGAAGAGTCCGGGATCCCTTACCAAAGTGGATTGGTTCGTTCTCATTACATA
GGTCGAACCTTTATCGAACCGGACCAAAAAATCCGAGACTTTGGAGCCAAAATCAAATACAATGTGGTAAAGGAAGTTGT
GAACGGAAAACGAGTGGTCATCATCGACGACTCGGTAATGCGTGGAACCACAAGCCGTAAAATCATCAAAATGATTCGAA
ATGCTGGTGCCAAAGAAATCCATTTTCGAGTGTCCGCACCTCCAACGGTGGCTCCTTGTTATTATGGAATCGATATTCCA
ACTCACAAAGAACTCATTGCATCAACCCATAGCATTGAGGAAATCCAGAAATACTTACGTGTGGACTCCCTCGCCTACCT
TACGTTAGATAGGATGCACAAAGCAGTGGAAGGCCACAAAGGTGGTGGATTTTGTGATGCTTGTTTTACATCCAATTATC
CAGTAGAATTCCAAGACCACGCGGGAAACCAAAAGTCACTATTTACGGAATACGCAACGGAAGAGTGA

Upstream 100 bases:

>100_bases
AGGGCCAAATCCAAAGCTTTTGCTGTATCGACGAGAATATAGTTGGAATTGATTTGCATCTAATTGGGATTATGGAAATA
CTGCAATTGTCATTCTAACA

Downstream 100 bases:

>100_bases
TGGAAGAGATCGAACTTTCCAAAACCTTTGGTTTTGAAGCTGCCCATTTTTTACCGAATGTTCCAGAAGGACACAAATGC
AAACGGATGCATGGCCACAG

Product: glutamine phosphoribosylpyrophosphate amidotransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MILQSDKPKEECAIYGIYNSKEAANFTYLGLYSMQHRGQESSGIVSTDGSHLYRYANMGLVANIFTQPKIKELIGDAAIG
HNRYSTTGASFLRNAQPVRVESHLGPVALAHNGNLVNSWDIRNRLERDGSIFQTTIDSEVIVHLMAKSHKTDLLEALCES
LAQVRGAYSLLVLTPRYLIAVRDPNGFRPLVMGKRSDGAIVFASETCAFDITDTEYVRDVEPGEMVVIDHTGMRSLYPFP
KAKPSLCIFEYIYFARPDSYIFEESVYKVRKSLGRQLARVMPVEADVVIPVPDSANIAALGYSEESGIPYQSGLVRSHYI
GRTFIEPDQKIRDFGAKIKYNVVKEVVNGKRVVIIDDSVMRGTTSRKIIKMIRNAGAKEIHFRVSAPPTVAPCYYGIDIP
THKELIASTHSIEEIQKYLRVDSLAYLTLDRMHKAVEGHKGGGFCDACFTSNYPVEFQDHAGNQKSLFTEYATEE

Sequences:

>Translated_475_residues
MILQSDKPKEECAIYGIYNSKEAANFTYLGLYSMQHRGQESSGIVSTDGSHLYRYANMGLVANIFTQPKIKELIGDAAIG
HNRYSTTGASFLRNAQPVRVESHLGPVALAHNGNLVNSWDIRNRLERDGSIFQTTIDSEVIVHLMAKSHKTDLLEALCES
LAQVRGAYSLLVLTPRYLIAVRDPNGFRPLVMGKRSDGAIVFASETCAFDITDTEYVRDVEPGEMVVIDHTGMRSLYPFP
KAKPSLCIFEYIYFARPDSYIFEESVYKVRKSLGRQLARVMPVEADVVIPVPDSANIAALGYSEESGIPYQSGLVRSHYI
GRTFIEPDQKIRDFGAKIKYNVVKEVVNGKRVVIIDDSVMRGTTSRKIIKMIRNAGAKEIHFRVSAPPTVAPCYYGIDIP
THKELIASTHSIEEIQKYLRVDSLAYLTLDRMHKAVEGHKGGGFCDACFTSNYPVEFQDHAGNQKSLFTEYATEE
>Mature_475_residues
MILQSDKPKEECAIYGIYNSKEAANFTYLGLYSMQHRGQESSGIVSTDGSHLYRYANMGLVANIFTQPKIKELIGDAAIG
HNRYSTTGASFLRNAQPVRVESHLGPVALAHNGNLVNSWDIRNRLERDGSIFQTTIDSEVIVHLMAKSHKTDLLEALCES
LAQVRGAYSLLVLTPRYLIAVRDPNGFRPLVMGKRSDGAIVFASETCAFDITDTEYVRDVEPGEMVVIDHTGMRSLYPFP
KAKPSLCIFEYIYFARPDSYIFEESVYKVRKSLGRQLARVMPVEADVVIPVPDSANIAALGYSEESGIPYQSGLVRSHYI
GRTFIEPDQKIRDFGAKIKYNVVKEVVNGKRVVIIDDSVMRGTTSRKIIKMIRNAGAKEIHFRVSAPPTVAPCYYGIDIP
THKELIASTHSIEEIQKYLRVDSLAYLTLDRMHKAVEGHKGGGFCDACFTSNYPVEFQDHAGNQKSLFTEYATEE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=517, Percent_Identity=37.5241779497099, Blast_Score=345, Evalue=6e-95,
Organism=Escherichia coli, GI1788651, Length=454, Percent_Identity=36.784140969163, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1790167, Length=188, Percent_Identity=30.8510638297872, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=39.2484342379958, Blast_Score=333, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6323958, Length=458, Percent_Identity=35.8078602620087, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI28573187, Length=492, Percent_Identity=39.0243902439024, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI24659598, Length=484, Percent_Identity=38.6363636363636, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24659604, Length=460, Percent_Identity=39.5652173913043, Blast_Score=330, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53073; Mature: 53073

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILQSDKPKEECAIYGIYNSKEAANFTYLGLYSMQHRGQESSGIVSTDGSHLYRYANMGL
CCCCCCCCHHHEEEEEEECCCCCCCEEEEEEEHHHHCCCCCCCEEECCCHHEEEECCCCE
VANIFTQPKIKELIGDAAIGHNRYSTTGASFLRNAQPVRVESHLGPVALAHNGNLVNSWD
EEEHHCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEECCCCCEEEECCCCEEECHH
IRNRLERDGSIFQTTIDSEVIVHLMAKSHKTDLLEALCESLAQVRGAYSLLVLTPRYLIA
HHHHHHCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCEEEEE
VRDPNGFRPLVMGKRSDGAIVFASETCAFDITDTEYVRDVEPGEMVVIDHTGMRSLYPFP
EECCCCCCCEEEECCCCCEEEEEECCEEEECCCHHHHHCCCCCCEEEEECCCCHHCCCCC
KAKPSLCIFEYIYFARPDSYIFEESVYKVRKSLGRQLARVMPVEADVVIPVPDSANIAAL
CCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEE
GYSEESGIPYQSGLVRSHYIGRTFIEPDQKIRDFGAKIKYNVVKEVVNGKRVVIIDDSVM
ECCCCCCCCHHHCCHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCEEEEECCHHH
RGTTSRKIIKMIRNAGAKEIHFRVSAPPTVAPCYYGIDIPTHKELIASTHSIEEIQKYLR
CCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHH
VDSLAYLTLDRMHKAVEGHKGGGFCDACFTSNYPVEFQDHAGNQKSLFTEYATEE
HHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCEEECCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MILQSDKPKEECAIYGIYNSKEAANFTYLGLYSMQHRGQESSGIVSTDGSHLYRYANMGL
CCCCCCCCHHHEEEEEEECCCCCCCEEEEEEEHHHHCCCCCCCEEECCCHHEEEECCCCE
VANIFTQPKIKELIGDAAIGHNRYSTTGASFLRNAQPVRVESHLGPVALAHNGNLVNSWD
EEEHHCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEECCCCCEEEECCCCEEECHH
IRNRLERDGSIFQTTIDSEVIVHLMAKSHKTDLLEALCESLAQVRGAYSLLVLTPRYLIA
HHHHHHCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCEEEEE
VRDPNGFRPLVMGKRSDGAIVFASETCAFDITDTEYVRDVEPGEMVVIDHTGMRSLYPFP
EECCCCCCCEEEECCCCCEEEEEECCEEEECCCHHHHHCCCCCCEEEEECCCCHHCCCCC
KAKPSLCIFEYIYFARPDSYIFEESVYKVRKSLGRQLARVMPVEADVVIPVPDSANIAAL
CCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEE
GYSEESGIPYQSGLVRSHYIGRTFIEPDQKIRDFGAKIKYNVVKEVVNGKRVVIIDDSVM
ECCCCCCCCHHHCCHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCEEEEECCHHH
RGTTSRKIIKMIRNAGAKEIHFRVSAPPTVAPCYYGIDIPTHKELIASTHSIEEIQKYLR
CCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHHH
VDSLAYLTLDRMHKAVEGHKGGGFCDACFTSNYPVEFQDHAGNQKSLFTEYATEE
HHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCEEECCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]