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The map label for this gene is gmd [H]

Identifier: 183219589

GI number: 183219589

Start: 161146

End: 162162

Strand: Direct

Name: gmd [H]

Synonym: LEPBI_I0164

Alternate gene names: 183219589

Gene position: 161146-162162 (Clockwise)

Preceding gene: 183219586

Following gene: 183219591

Centisome position: 4.48

GC content: 40.61

Gene sequence:

>1017_bases
ATGAAAAAAGCACTCATCACGGGAATCACGGGCCAAGATGGTTCCTACTTAGCAGAACTCCTCCTCCAAAAAGGATACGA
AGTCCATGGTATCGTTCGTAGAACGAGTTTGTTCAATCGCAATCGGATTGAACAACTCCACGGAAACCCAAACCTATTTT
TGCACTATGGGGACCTTACCGATTCATCCAATTTGAATCGAATCTTAGAAAAAATCCAACCATCCGAAATTTACAATTTG
GCGGCACAGTCCCATGTTCAGGTTTCGTTTGAAGTGCCAGAATATACGGCAGAAGTTGATGCTGTAGGAACTTTAAGGAT
CTTAGATGCCATCAAACAAACAGGCATTAGCTCAAGGTTCTACCAAGCTTCTACTTCTGAGTTGTATGGTCTTGTACAAG
AAGTGCCACAAACAGAAAAAACTCCATTTTACCCACGTTCCCCTTATGCTGTCGCAAAACTCTATGCCTACTGGGCAGTT
GTGAACTATCGTGAAGCTTACAATTTACATGCATCCAATGGAATTTTATTCAACCATGAATCTCCAAGACGTGGTGAAAC
CTTTGTCACTCGTAAAATCACTCTCGGTGTTTCTGCCGTCAAAGCAGGAAAACTTCCTTTCATCACAATGGGAAACATTG
ATTCCAAACGAGATTGGGGTTATGCACCAGATTATGTTGAGATGATGTGGATGATGTTACAACAAGACAAACCTGATGAT
TATGTTGTTGCTACCAATGAAACACATACAGTTCGCGAATTCATCGAAGAATCTTATAAGATTGCAGGTTATGAAGTGGT
ATGGGAAGGCAAAGAAGACAATGAAATTGGAAAGGACAAAAAAACTGGCCAAGTTCTAGTCAAAATTGATCCAAAATACT
ACCGACCTACAGAAGTAGAACTTCTCATTGGAAATCCAGAGAAGGCAAAACGTCAGTTAGGTTGGGAGCCAAAAGTAAAG
TTCAAAGAACTTGTCAAAATTATGATGGAAGCGGACTTAAAGGCACAAGGATTTTAG

Upstream 100 bases:

>100_bases
AAGGCAAAGGGAATTCGGTGGGTATTTGCAGATAATTCATCGTCCCCATAGCAATTTTCCTTTTCTTTCGAAAGTATTTT
AAAAAACTGTTCTCTGATCT

Downstream 100 bases:

>100_bases
GCGAATCCTGAATTTAAAAAAAATCATGAACAACAATAGGATTACTAAAAATCCTATTGTTGGAAGACCGTTGAATTGAT
TGAATAAAATCAAATTCAAA

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKKALITGITGQDGSYLAELLLQKGYEVHGIVRRTSLFNRNRIEQLHGNPNLFLHYGDLTDSSNLNRILEKIQPSEIYNL
AAQSHVQVSFEVPEYTAEVDAVGTLRILDAIKQTGISSRFYQASTSELYGLVQEVPQTEKTPFYPRSPYAVAKLYAYWAV
VNYREAYNLHASNGILFNHESPRRGETFVTRKITLGVSAVKAGKLPFITMGNIDSKRDWGYAPDYVEMMWMMLQQDKPDD
YVVATNETHTVREFIEESYKIAGYEVVWEGKEDNEIGKDKKTGQVLVKIDPKYYRPTEVELLIGNPEKAKRQLGWEPKVK
FKELVKIMMEADLKAQGF

Sequences:

>Translated_338_residues
MKKALITGITGQDGSYLAELLLQKGYEVHGIVRRTSLFNRNRIEQLHGNPNLFLHYGDLTDSSNLNRILEKIQPSEIYNL
AAQSHVQVSFEVPEYTAEVDAVGTLRILDAIKQTGISSRFYQASTSELYGLVQEVPQTEKTPFYPRSPYAVAKLYAYWAV
VNYREAYNLHASNGILFNHESPRRGETFVTRKITLGVSAVKAGKLPFITMGNIDSKRDWGYAPDYVEMMWMMLQQDKPDD
YVVATNETHTVREFIEESYKIAGYEVVWEGKEDNEIGKDKKTGQVLVKIDPKYYRPTEVELLIGNPEKAKRQLGWEPKVK
FKELVKIMMEADLKAQGF
>Mature_338_residues
MKKALITGITGQDGSYLAELLLQKGYEVHGIVRRTSLFNRNRIEQLHGNPNLFLHYGDLTDSSNLNRILEKIQPSEIYNL
AAQSHVQVSFEVPEYTAEVDAVGTLRILDAIKQTGISSRFYQASTSELYGLVQEVPQTEKTPFYPRSPYAVAKLYAYWAV
VNYREAYNLHASNGILFNHESPRRGETFVTRKITLGVSAVKAGKLPFITMGNIDSKRDWGYAPDYVEMMWMMLQQDKPDD
YVVATNETHTVREFIEESYKIAGYEVVWEGKEDNEIGKDKKTGQVLVKIDPKYYRPTEVELLIGNPEKAKRQLGWEPKVK
FKELVKIMMEADLKAQGF

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=339, Percent_Identity=63.716814159292, Blast_Score=463, Evalue=1e-131,
Organism=Homo sapiens, GI42516563, Length=327, Percent_Identity=25.6880733944954, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=25.4437869822485, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=58.1196581196581, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=24.7910863509749, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI48994969, Length=343, Percent_Identity=26.530612244898, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=341, Percent_Identity=63.049853372434, Blast_Score=449, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=62.7565982404692, Blast_Score=445, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI133901788, Length=341, Percent_Identity=62.7565982404692, Blast_Score=445, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=62.7565982404692, Blast_Score=445, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17539422, Length=341, Percent_Identity=62.7565982404692, Blast_Score=445, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=62.7565982404692, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24158427, Length=341, Percent_Identity=63.3431085043988, Blast_Score=460, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38634; Mature: 38634

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALITGITGQDGSYLAELLLQKGYEVHGIVRRTSLFNRNRIEQLHGNPNLFLHYGDLT
CCCCEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
DSSNLNRILEKIQPSEIYNLAAQSHVQVSFEVPEYTAEVDAVGTLRILDAIKQTGISSRF
CCCCHHHHHHHCCHHHHHHHHHCCEEEEEEECCCHHHHHCCCHHHHHHHHHHHCCCCCHH
YQASTSELYGLVQEVPQTEKTPFYPRSPYAVAKLYAYWAVVNYREAYNLHASNGILFNHE
HHCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCC
SPRRGETFVTRKITLGVSAVKAGKLPFITMGNIDSKRDWGYAPDYVEMMWMMLQQDKPDD
CCCCCCEEEEEEEEECHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
YVVATNETHTVREFIEESYKIAGYEVVWEGKEDNEIGKDKKTGQVLVKIDPKYYRPTEVE
EEEEECCCHHHHHHHHHHHEECCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEE
LLIGNPEKAKRQLGWEPKVKFKELVKIMMEADLKAQGF
EEECCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKALITGITGQDGSYLAELLLQKGYEVHGIVRRTSLFNRNRIEQLHGNPNLFLHYGDLT
CCCCEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
DSSNLNRILEKIQPSEIYNLAAQSHVQVSFEVPEYTAEVDAVGTLRILDAIKQTGISSRF
CCCCHHHHHHHCCHHHHHHHHHCCEEEEEEECCCHHHHHCCCHHHHHHHHHHHCCCCCHH
YQASTSELYGLVQEVPQTEKTPFYPRSPYAVAKLYAYWAVVNYREAYNLHASNGILFNHE
HHCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCC
SPRRGETFVTRKITLGVSAVKAGKLPFITMGNIDSKRDWGYAPDYVEMMWMMLQQDKPDD
CCCCCCEEEEEEEEECHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
YVVATNETHTVREFIEESYKIAGYEVVWEGKEDNEIGKDKKTGQVLVKIDPKYYRPTEVE
EEEEECCCHHHHHHHHHHHEECCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEE
LLIGNPEKAKRQLGWEPKVKFKELVKIMMEADLKAQGF
EEECCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]