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The map label for this gene is glpF [H]

Identifier: 183219567

GI number: 183219567

Start: 138437

End: 139153

Strand: Reverse

Name: glpF [H]

Synonym: LEPBI_I0142

Alternate gene names: 183219567

Gene position: 139153-138437 (Counterclockwise)

Preceding gene: 183219571

Following gene: 183219566

Centisome position: 3.87

GC content: 49.09

Gene sequence:

>717_bases
TTGGAACTGGTTGGCGAATTTTTTGGAACGGCTGTACTGATCCTTTTAGGCGATGGAGTTGTCGCAGGCGTTTTATTGGA
GAAAACAAAATCCAAAGACGCAGGTTGGATCACCATCACAACGGCTTGGGCTCTTGCTGTATGTTTTGGCGTTCTTGTCG
CAAAAGCACTCGGTAGCCCTGGTGCACATCTGAATCCCGCTGTGACACTTTCAGTTTGCATCCAATCGGGAGAATATTCG
ATCTTTTTACCGTATGCAGTGGCACAAGTGTCTGGAGCTTCCCTCGGTGCCTTTTTGGTTTACTTACACTACCTTCCCCA
TTGGAAAGAAACAAAAGACCCAGGGAAAATCTTGGCCGTATTTTCCACAGAACCTGCCATCAAACACACTCTCTCCAATG
TCATCAGTGAAGGGTTCGGAACCTTCCTTCTCATCCTTGGTATCCATGCGATTTTTTCGCCCTTAAATGGTGGCGCCAGT
GGGATCGCAGGTACAGGCTTTGTGGCTCTCCTCGTTTGGGCCATTGGACTTTCGATGGGAGGCACTACTGGGTATGCGAT
TAACCCAGCTCGTGACCTTGGTCCAAGGATCATCCATTCACTGCTTCCAATACCCAATAAAGGTGACTCTGGTTGGAAGT
ATGCTTGGCTTCCCGTAGTGATTCCGTTAGTGGGTGGTGGACTTGCGGCAGTGGTGATCCGGTGGGTGATTGTATGA

Upstream 100 bases:

>100_bases
AATCCTAGAATAAATTGAGAGAAAGCACCTCCAAAAAAGGTTGCTCTATGTTCTATGTCCCTTCAGAGTAAGTCCTCATC
GAAGTCCGGGGGAGGGCATT

Downstream 100 bases:

>100_bases
AAAAAATTGCTTTCACACTATTACTCATTGGAATCTCAATTTACGGCCAATCAACAACTATGTTCAAAACAGTGAAGTTG
ATATGAAGTCTTATATCCAA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MELVGEFFGTAVLILLGDGVVAGVLLEKTKSKDAGWITITTAWALAVCFGVLVAKALGSPGAHLNPAVTLSVCIQSGEYS
IFLPYAVAQVSGASLGAFLVYLHYLPHWKETKDPGKILAVFSTEPAIKHTLSNVISEGFGTFLLILGIHAIFSPLNGGAS
GIAGTGFVALLVWAIGLSMGGTTGYAINPARDLGPRIIHSLLPIPNKGDSGWKYAWLPVVIPLVGGGLAAVVIRWVIV

Sequences:

>Translated_238_residues
MELVGEFFGTAVLILLGDGVVAGVLLEKTKSKDAGWITITTAWALAVCFGVLVAKALGSPGAHLNPAVTLSVCIQSGEYS
IFLPYAVAQVSGASLGAFLVYLHYLPHWKETKDPGKILAVFSTEPAIKHTLSNVISEGFGTFLLILGIHAIFSPLNGGAS
GIAGTGFVALLVWAIGLSMGGTTGYAINPARDLGPRIIHSLLPIPNKGDSGWKYAWLPVVIPLVGGGLAAVVIRWVIV
>Mature_238_residues
MELVGEFFGTAVLILLGDGVVAGVLLEKTKSKDAGWITITTAWALAVCFGVLVAKALGSPGAHLNPAVTLSVCIQSGEYS
IFLPYAVAQVSGASLGAFLVYLHYLPHWKETKDPGKILAVFSTEPAIKHTLSNVISEGFGTFLLILGIHAIFSPLNGGAS
GIAGTGFVALLVWAIGLSMGGTTGYAINPARDLGPRIIHSLLPIPNKGDSGWKYAWLPVVIPLVGGGLAAVVIRWVIV

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=237, Percent_Identity=36.7088607594937, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI157266307, Length=249, Percent_Identity=35.7429718875502, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI22538420, Length=236, Percent_Identity=33.4745762711864, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4502187, Length=244, Percent_Identity=31.1475409836066, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1790362, Length=254, Percent_Identity=31.1023622047244, Blast_Score=81, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17544068, Length=252, Percent_Identity=32.1428571428571, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71994009, Length=231, Percent_Identity=31.1688311688312, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17531429, Length=237, Percent_Identity=32.0675105485232, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531431, Length=237, Percent_Identity=32.0675105485232, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321054, Length=219, Percent_Identity=32.8767123287671, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24770; Mature: 24770

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELVGEFFGTAVLILLGDGVVAGVLLEKTKSKDAGWITITTAWALAVCFGVLVAKALGSP
CCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCC
GAHLNPAVTLSVCIQSGEYSIFLPYAVAQVSGASLGAFLVYLHYLPHWKETKDPGKILAV
CCCCCHHEEEEEEEECCCEEEECCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEE
FSTEPAIKHTLSNVISEGFGTFLLILGIHAIFSPLNGGASGIAGTGFVALLVWAIGLSMG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
GTTGYAINPARDLGPRIIHSLLPIPNKGDSGWKYAWLPVVIPLVGGGLAAVVIRWVIV
CCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MELVGEFFGTAVLILLGDGVVAGVLLEKTKSKDAGWITITTAWALAVCFGVLVAKALGSP
CCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCC
GAHLNPAVTLSVCIQSGEYSIFLPYAVAQVSGASLGAFLVYLHYLPHWKETKDPGKILAV
CCCCCHHEEEEEEEECCCEEEECCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEE
FSTEPAIKHTLSNVISEGFGTFLLILGIHAIFSPLNGGASGIAGTGFVALLVWAIGLSMG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
GTTGYAINPARDLGPRIIHSLLPIPNKGDSGWKYAWLPVVIPLVGGGLAAVVIRWVIV
CCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7565084; 11463916 [H]