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The map label for this gene is fixL [H]

Identifier: 183219520

GI number: 183219520

Start: 93732

End: 95351

Strand: Direct

Name: fixL [H]

Synonym: LEPBI_I0093

Alternate gene names: 183219520

Gene position: 93732-95351 (Clockwise)

Preceding gene: 183219517

Following gene: 183219521

Centisome position: 2.6

GC content: 36.91

Gene sequence:

>1620_bases
TTGATGCCAACTAATTCTGCTATATCCCGTATTTGGCAAAACCCTTCTGCCTTCCCTCACGAAGAGGTACTTCGGGAATT
AGAAAACTTACTCAGATCCAATCCAGATTTTTGGCTCAAAATCAACCGAGATGGAATCATCGTTGATTATAAAACCTCTC
GGTTTGTGAACATTGGAGAAAAACCAGAGCATTTACTTGGCAAACACATAAATGAGGGACTTCCTCCTTATCTACGAGAT
ATCACTTCAGAAGCCTTAGAATACTTAACTGACAAAAAAAGTTTGGTATTTTGGAAAGAGTATTCTTACGGAGTTGCACC
TAATATTCGCCATGTGGAAGTTCGTTTTGTTGTTTTGTATGATGGATTTATCATGTCCAACCATCGTGACATTACAGAAC
GAAAACGACTAGAGAATGCGTTTTTAGAAAGTGAGTCCAGATTTTTATCCATGGCACAAAATGCTGCTGATTCCATCATC
ATTATCAATGACGAAGGGATCATTCAGTTTTTTAATAAAACTGCGGAGGATACATTTGGTTACAAACAAGAAGATGTGAT
TGGTAAAAATGTTACGATGATCATACCTCCTGAATACAAAGGAAAACACGATACCTTTTTAAAAAGGTATAAGGATACAG
AAGAAAATCATATCATTGGGGTCGGTCGGGAAGTTTTCGCACAACGGATGTCAGGTGAAGTATTTCCATGTGAACTTTCA
GTGGGTGAGTTCAAAACCAAAACAGGGAAAATGTTTACTGGCATTCTCCGAGATATCAGTCAAAGAAAACTCCAAGAAGA
AGAACTTTACCAATATAGAAATCATTTAGAAGATCTGGTCGAAAGCCAAACCATGGACTTAAAAATGTCCAAAAATATTG
CGGAAGAGGCTTCTTACATGAAATCTCTTTTTCTTGCAAATATTTCTCATGAGTTAAAAACACCCATCCATGCCATTTTG
AGTTACGCTGAACTGGGTGCAGAGAAATCAGCGAGTGTTTCTCCTGAAAAAATAAAAGAATACTTTCAAATCATCGATTC
TTCCGGCAAACGATTACTTTCTCTTTTAGAAAACCTACTCGATATCGCCAAACTAGAGTCAGGAAAGATGCGATATTTGT
TTGAAAAGAATTGTTTAAAAGAAACAACTAAGTTTGTGATCAATGAAATGCGTGCAATTTTGGAAAAAAGAGGGATCACA
GTTGTTTTACTGGATAAGGAAGAACGTTGGGAAGCGGAATTCGATTATGAGCGCATCCAACAAGTGATTCGAAATATCAT
CTCCAATGCATTAAAATTCATTCCAAATGATACTAATATAGAGATCAGTTTGATAAAAAGAGAATTTATTCCTAGAAAAT
CTCAGTCCTATGTCAAAGGGATTGGCATACAGATTCGAGATTTTGGACCAGGAATTCCAGCGGAAGACCTGGATAAAATT
TTTGAAAAATTCATCCAATCCAAACAAGTGAAAGCGGGAACAAAAGGAACAGGACTCGGTTTGTCAATCTCAAGAGAAAT
AGTGAATGATCATAACGGCTTGTTGTATGCAGAAAATCATGAAGAAAAGGGAGCCGTATTTACCATGTTAATACCATGTT
CCCGTGAAGGAATTCGATGA

Upstream 100 bases:

>100_bases
CAAGGGAAAAATCCTTACGTTGATTCTTTTTTCCCCATGATTTCTTACAATTTCTTTCCGAAAATGAGATTGTTTCCAAT
ATTCCTAATGAGGTCAGTAC

Downstream 100 bases:

>100_bases
TCAATTTACTTGCTGTTGATGATGAAATGATCAATTTGATGATCATTGATGAATCTTTGTCTGACAAGGGATTCACTGTT
GTAAAGGCAAAAGACGGGGA

Product: putative sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MMPTNSAISRIWQNPSAFPHEEVLRELENLLRSNPDFWLKINRDGIIVDYKTSRFVNIGEKPEHLLGKHINEGLPPYLRD
ITSEALEYLTDKKSLVFWKEYSYGVAPNIRHVEVRFVVLYDGFIMSNHRDITERKRLENAFLESESRFLSMAQNAADSII
IINDEGIIQFFNKTAEDTFGYKQEDVIGKNVTMIIPPEYKGKHDTFLKRYKDTEENHIIGVGREVFAQRMSGEVFPCELS
VGEFKTKTGKMFTGILRDISQRKLQEEELYQYRNHLEDLVESQTMDLKMSKNIAEEASYMKSLFLANISHELKTPIHAIL
SYAELGAEKSASVSPEKIKEYFQIIDSSGKRLLSLLENLLDIAKLESGKMRYLFEKNCLKETTKFVINEMRAILEKRGIT
VVLLDKEERWEAEFDYERIQQVIRNIISNALKFIPNDTNIEISLIKREFIPRKSQSYVKGIGIQIRDFGPGIPAEDLDKI
FEKFIQSKQVKAGTKGTGLGLSISREIVNDHNGLLYAENHEEKGAVFTMLIPCSREGIR

Sequences:

>Translated_539_residues
MMPTNSAISRIWQNPSAFPHEEVLRELENLLRSNPDFWLKINRDGIIVDYKTSRFVNIGEKPEHLLGKHINEGLPPYLRD
ITSEALEYLTDKKSLVFWKEYSYGVAPNIRHVEVRFVVLYDGFIMSNHRDITERKRLENAFLESESRFLSMAQNAADSII
IINDEGIIQFFNKTAEDTFGYKQEDVIGKNVTMIIPPEYKGKHDTFLKRYKDTEENHIIGVGREVFAQRMSGEVFPCELS
VGEFKTKTGKMFTGILRDISQRKLQEEELYQYRNHLEDLVESQTMDLKMSKNIAEEASYMKSLFLANISHELKTPIHAIL
SYAELGAEKSASVSPEKIKEYFQIIDSSGKRLLSLLENLLDIAKLESGKMRYLFEKNCLKETTKFVINEMRAILEKRGIT
VVLLDKEERWEAEFDYERIQQVIRNIISNALKFIPNDTNIEISLIKREFIPRKSQSYVKGIGIQIRDFGPGIPAEDLDKI
FEKFIQSKQVKAGTKGTGLGLSISREIVNDHNGLLYAENHEEKGAVFTMLIPCSREGIR
>Mature_539_residues
MMPTNSAISRIWQNPSAFPHEEVLRELENLLRSNPDFWLKINRDGIIVDYKTSRFVNIGEKPEHLLGKHINEGLPPYLRD
ITSEALEYLTDKKSLVFWKEYSYGVAPNIRHVEVRFVVLYDGFIMSNHRDITERKRLENAFLESESRFLSMAQNAADSII
IINDEGIIQFFNKTAEDTFGYKQEDVIGKNVTMIIPPEYKGKHDTFLKRYKDTEENHIIGVGREVFAQRMSGEVFPCELS
VGEFKTKTGKMFTGILRDISQRKLQEEELYQYRNHLEDLVESQTMDLKMSKNIAEEASYMKSLFLANISHELKTPIHAIL
SYAELGAEKSASVSPEKIKEYFQIIDSSGKRLLSLLENLLDIAKLESGKMRYLFEKNCLKETTKFVINEMRAILEKRGIT
VVLLDKEERWEAEFDYERIQQVIRNIISNALKFIPNDTNIEISLIKREFIPRKSQSYVKGIGIQIRDFGPGIPAEDLDKI
FEKFIQSKQVKAGTKGTGLGLSISREIVNDHNGLLYAENHEEKGAVFTMLIPCSREGIR

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=257, Percent_Identity=32.6848249027237, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI145693157, Length=241, Percent_Identity=29.4605809128631, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI87081816, Length=277, Percent_Identity=30.3249097472924, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1788713, Length=260, Percent_Identity=29.2307692307692, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1786600, Length=389, Percent_Identity=22.879177377892, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788549, Length=400, Percent_Identity=25.75, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI48994928, Length=261, Percent_Identity=26.8199233716475, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786912, Length=278, Percent_Identity=27.6978417266187, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=24.8908296943231, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788393, Length=261, Percent_Identity=22.9885057471264, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=249, Percent_Identity=27.3092369477912, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790346, Length=238, Percent_Identity=26.0504201680672, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081921, Length=135, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790551, Length=224, Percent_Identity=23.6607142857143, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786783, Length=247, Percent_Identity=23.4817813765182, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 62172; Mature: 62172

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPTNSAISRIWQNPSAFPHEEVLRELENLLRSNPDFWLKINRDGIIVDYKTSRFVNIGE
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCEEECCC
KPEHLLGKHINEGLPPYLRDITSEALEYLTDKKSLVFWKEYSYGVAPNIRHVEVRFVVLY
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEEEEE
DGFIMSNHRDITERKRLENAFLESESRFLSMAQNAADSIIIINDEGIIQFFNKTAEDTFG
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCC
YKQEDVIGKNVTMIIPPEYKGKHDTFLKRYKDTEENHIIGVGREVFAQRMSGEVFPCELS
CCCHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCEEEEEEC
VGEFKTKTGKMFTGILRDISQRKLQEEELYQYRNHLEDLVESQTMDLKMSKNIAEEASYM
CCCHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KSLFLANISHELKTPIHAILSYAELGAEKSASVSPEKIKEYFQIIDSSGKRLLSLLENLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
DIAKLESGKMRYLFEKNCLKETTKFVINEMRAILEKRGITVVLLDKEERWEAEFDYERIQ
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHH
QVIRNIISNALKFIPNDTNIEISLIKREFIPRKSQSYVKGIGIQIRDFGPGIPAEDLDKI
HHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCHHHHHHHCCEEEEECCCCCCHHHHHHH
FEKFIQSKQVKAGTKGTGLGLSISREIVNDHNGLLYAENHEEKGAVFTMLIPCSREGIR
HHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MMPTNSAISRIWQNPSAFPHEEVLRELENLLRSNPDFWLKINRDGIIVDYKTSRFVNIGE
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCEEECCC
KPEHLLGKHINEGLPPYLRDITSEALEYLTDKKSLVFWKEYSYGVAPNIRHVEVRFVVLY
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEEEEE
DGFIMSNHRDITERKRLENAFLESESRFLSMAQNAADSIIIINDEGIIQFFNKTAEDTFG
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCC
YKQEDVIGKNVTMIIPPEYKGKHDTFLKRYKDTEENHIIGVGREVFAQRMSGEVFPCELS
CCCHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCEEEEEEC
VGEFKTKTGKMFTGILRDISQRKLQEEELYQYRNHLEDLVESQTMDLKMSKNIAEEASYM
CCCHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KSLFLANISHELKTPIHAILSYAELGAEKSASVSPEKIKEYFQIIDSSGKRLLSLLENLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
DIAKLESGKMRYLFEKNCLKETTKFVINEMRAILEKRGITVVLLDKEERWEAEFDYERIQ
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHH
QVIRNIISNALKFIPNDTNIEISLIKREFIPRKSQSYVKGIGIQIRDFGPGIPAEDLDKI
HHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCHHHHHHHCCEEEEECCCCCCHHHHHHH
FEKFIQSKQVKAGTKGTGLGLSISREIVNDHNGLLYAENHEEKGAVFTMLIPCSREGIR
HHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]