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The map label for this gene is ispA [H]

Identifier: 183219510

GI number: 183219510

Start: 82822

End: 83745

Strand: Reverse

Name: ispA [H]

Synonym: LEPBI_I0083

Alternate gene names: 183219510

Gene position: 83745-82822 (Counterclockwise)

Preceding gene: 183219511

Following gene: 183219509

Centisome position: 2.33

GC content: 40.15

Gene sequence:

>924_bases
GTGCCAAATTCGTTCGAATCAATCCTACTAAAATCCAAACAAGTTTTTGATTCCTTTTTTGAATCCTACACAGCTAAACT
ATTTTCTCCCAAAACTCGCATAACAGAAGCATGCTTATATAGTTTACATGCAGGTGGAAAACGAGTCAGACCCATCTTTG
TAATCAATTCCTTTTTTGAACCAAATGATTTGTTTAGTTCAGAACCTTTTGATACTCACCAATCCGTTTTTTTAGCTGCA
CTCGCTGTAGAATGTATCCATACATATTCACTCATCCATGATGATTTACCTGCGATGGACAATGACGACACTCGTCGCGG
AAAACCAACTTGCCACAAACAATTTGATGAAGCCACTGCAATCCTTGCTGGTGACACTCTCAATTCATTGAGTTTTCAAT
TATTATCCATGATGGATGCAAACGATCCGAACCTCCTTCGTGACTCCATTCAGATCTTACACCAAGGGGCCGGGATGACT
GGAATGATCCTCGGACAGATGGAAGACATCGAGGAAGAAAAAAATCCGAGTTCCAATGCTCGTGAATCCAAACTCGAATC
CATTCATGAAAAAAAGACAGGCGCCCTCATCACATCTTCATTTCTTTTGGGAAACCGATTACGAGCGGATTGGAAAGAGA
GAGAATTCGTACTCACTCGTTATGCGAAAGAAATCGGACTCCTTTTCCAAATCACAGACGATATTCTCGATGTGGAAGGG
AATTTAGCAGAACTTGGAAAAACACCTGGAAAAGATGCAAAAGCTGGAAAATTGACCTATCCAAGTTTGTATGGTTTGGA
AAAATCAAAATTACTTCGCGACCAAACGCTTGGTAAGGCCATTTCTTTGGCAGCGGAACTTCCCTCTTTACACAATGAAT
TCTTTTTAGGATTGCCTAAGTACATTGCTGAAAGAAAAAATTAG

Upstream 100 bases:

>100_bases
CATTACAAGAAATTGCATTTTTCTTCAAAGGGTATGAGCTTAACTAAGCTTTTGATTTACACTCAGATCGGTTCTTAATT
CAGAACCGGTCTCACCCATC

Downstream 100 bases:

>100_bases
ACTCGATGATTATCTCGTTCGCGAAGGTTATGCGAAAGATTTAAAATTAGCACAATCATTAATCCTATCAGGATCGGTAC
TCGTCAATGATACTATCGTC

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPNSFESILLKSKQVFDSFFESYTAKLFSPKTRITEACLYSLHAGGKRVRPIFVINSFFEPNDLFSSEPFDTHQSVFLAA
LAVECIHTYSLIHDDLPAMDNDDTRRGKPTCHKQFDEATAILAGDTLNSLSFQLLSMMDANDPNLLRDSIQILHQGAGMT
GMILGQMEDIEEEKNPSSNARESKLESIHEKKTGALITSSFLLGNRLRADWKEREFVLTRYAKEIGLLFQITDDILDVEG
NLAELGKTPGKDAKAGKLTYPSLYGLEKSKLLRDQTLGKAISLAAELPSLHNEFFLGLPKYIAERKN

Sequences:

>Translated_307_residues
MPNSFESILLKSKQVFDSFFESYTAKLFSPKTRITEACLYSLHAGGKRVRPIFVINSFFEPNDLFSSEPFDTHQSVFLAA
LAVECIHTYSLIHDDLPAMDNDDTRRGKPTCHKQFDEATAILAGDTLNSLSFQLLSMMDANDPNLLRDSIQILHQGAGMT
GMILGQMEDIEEEKNPSSNARESKLESIHEKKTGALITSSFLLGNRLRADWKEREFVLTRYAKEIGLLFQITDDILDVEG
NLAELGKTPGKDAKAGKLTYPSLYGLEKSKLLRDQTLGKAISLAAELPSLHNEFFLGLPKYIAERKN
>Mature_306_residues
PNSFESILLKSKQVFDSFFESYTAKLFSPKTRITEACLYSLHAGGKRVRPIFVINSFFEPNDLFSSEPFDTHQSVFLAAL
AVECIHTYSLIHDDLPAMDNDDTRRGKPTCHKQFDEATAILAGDTLNSLSFQLLSMMDANDPNLLRDSIQILHQGAGMTG
MILGQMEDIEEEKNPSSNARESKLESIHEKKTGALITSSFLLGNRLRADWKEREFVLTRYAKEIGLLFQITDDILDVEGN
LAELGKTPGKDAKAGKLTYPSLYGLEKSKLLRDQTLGKAISLAAELPSLHNEFFLGLPKYIAERKN

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=251, Percent_Identity=27.8884462151394, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1786623, Length=276, Percent_Identity=43.1159420289855, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1789578, Length=254, Percent_Identity=27.5590551181102, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17505681, Length=252, Percent_Identity=28.1746031746032, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24651612, Length=230, Percent_Identity=26.5217391304348, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 34357; Mature: 34225

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2 ; PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNSFESILLKSKQVFDSFFESYTAKLFSPKTRITEACLYSLHAGGKRVRPIFVINSFFE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
PNDLFSSEPFDTHQSVFLAALAVECIHTYSLIHDDLPAMDNDDTRRGKPTCHKQFDEATA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHE
ILAGDTLNSLSFQLLSMMDANDPNLLRDSIQILHQGAGMTGMILGQMEDIEEEKNPSSNA
EEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCH
RESKLESIHEKKTGALITSSFLLGNRLRADWKEREFVLTRYAKEIGLLFQITDDILDVEG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEHHHHHCCCC
NLAELGKTPGKDAKAGKLTYPSLYGLEKSKLLRDQTLGKAISLAAELPSLHNEFFLGLPK
CHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
YIAERKN
HHHCCCC
>Mature Secondary Structure 
PNSFESILLKSKQVFDSFFESYTAKLFSPKTRITEACLYSLHAGGKRVRPIFVINSFFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
PNDLFSSEPFDTHQSVFLAALAVECIHTYSLIHDDLPAMDNDDTRRGKPTCHKQFDEATA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHE
ILAGDTLNSLSFQLLSMMDANDPNLLRDSIQILHQGAGMTGMILGQMEDIEEEKNPSSNA
EEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCH
RESKLESIHEKKTGALITSSFLLGNRLRADWKEREFVLTRYAKEIGLLFQITDDILDVEG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEHHHHHCCCC
NLAELGKTPGKDAKAGKLTYPSLYGLEKSKLLRDQTLGKAISLAAELPSLHNEFFLGLPK
CHHHHCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
YIAERKN
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]