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The map label for this gene is purB [H]

Identifier: 183219476

GI number: 183219476

Start: 47282

End: 48586

Strand: Reverse

Name: purB [H]

Synonym: LEPBI_I0049

Alternate gene names: 183219476

Gene position: 48586-47282 (Counterclockwise)

Preceding gene: 183219478

Following gene: 183219475

Centisome position: 1.35

GC content: 41.07

Gene sequence:

>1305_bases
ATGATCGATCGTTATAGCCATCCTGAAATTTCCGCCATTTGGGAATTAGAGAACAAATTTAAAATTTGGACAGATATTGA
AATTTATGCCTGCGAAGCTCGCGCCAATCGAGGAGAAGTCCCAAAAGAAGACCTCGAGACCATTAAACAAAAAGCAAAAT
TCAACGTGGATGAGATTTTAGAAATCGAATCGAAAGTCCATCATGATGTGATTGCTTATTTAACCAATTTAAACTCTTAT
ATAGGACCAGCTGGCCGTCATGTTCACTTTGGTCTTACTTCCAGTGATGTGGGTGATACTGCACTTTGTGTGCAAATGGT
GCAAGCAATGGATCTTCTCATCCAACGCACAGAAACACTTTTAGAAACCACAAAACAAAAAGCAAAAGAGTACAAAGACC
TTCCTTGTATCGGCCGGTCTCACGGGATCCATGCAGAACCGATGACCCTTGGTCTTAAGTTTGCACTCTTTTTTGCAGAG
ATGACTCGCAATTTAGAAAGGATGAAAGACGCACGGGAACAAGTGGCTGTCGGAAAACTATCTGGTGCGGTCGGAACATA
TTCCAATATCGATTTAGAAATTGAAGAATATGTATTGAACAAACTTGGATTAAAAGTAGATCCCATTGCAACACAGGTGA
TCTCAAGAGACCGACATGCTTTTTATATGTCAGTGCTTGGTGTTGTCGCTGCGAGTTTAGACCGAATGGCAACCGAGATT
CGATTGTTACAAAAAACGGAAGGGCGTGAAGTTGAAGAACCGTTTGCAAAAGGCCAAAAGGGATCATCCGCAATGCCTCA
CAAACGGAACCCCGTTGTTTGTGAGCGTATCTCTGGAATCTCTCGAGTCATTCGTTCTAACGTAAACGTGGGATTACAGA
ACGTGGGTCTTTGGCATGAACGAGATATCTCCCATTCCTCAGCGGAGCGAATTGTGCTTCCTGATTCCACCATTGCTCTG
GATTACATTTTGGAAAAAATGAATTTTGTATTGAAAGGTCTTCATGTGTATCCTGATGCCACCGAACGCACATTAAATGT
AACTCGCGGACTCATTTTCTCTCAAAAAGTATTATTGTGGCTCATTGAAAAAGGTGGGATCACAAGAGAAGATGCCTACT
TGATTGTCCAAGAAAATGCGATGGCTGTCTGGGCAGACCAATCTAAAAATTTGAGAGACCTTTTGAAACAAGATCCAAGA
TGTTCATCAATTCTCAAAGATTCTGACTTGGATGAAATTTTTCAAATCAAACCATATTTAGAGCGGATCCCACTGATTTT
CAAACGATTGGGAATCATAGATTAA

Upstream 100 bases:

>100_bases
CTAGGATTTATTACTAGTTTCTAAACATGAAACTCGAACATCGTTCGGCAATCGTAAAATTCCCAGTTGAAACGGAAGTT
TGCAATGAAAGACTGCCCTT

Downstream 100 bases:

>100_bases
CAAACATTAGAGAATCAATTTCTTAAAAATTTCAGATAGGTTAGGTTATGTGTACAATACAAAACCTAACCTTTTTTGTA
TCATCAGGTGTTGTCGTATA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MIDRYSHPEISAIWELENKFKIWTDIEIYACEARANRGEVPKEDLETIKQKAKFNVDEILEIESKVHHDVIAYLTNLNSY
IGPAGRHVHFGLTSSDVGDTALCVQMVQAMDLLIQRTETLLETTKQKAKEYKDLPCIGRSHGIHAEPMTLGLKFALFFAE
MTRNLERMKDAREQVAVGKLSGAVGTYSNIDLEIEEYVLNKLGLKVDPIATQVISRDRHAFYMSVLGVVAASLDRMATEI
RLLQKTEGREVEEPFAKGQKGSSAMPHKRNPVVCERISGISRVIRSNVNVGLQNVGLWHERDISHSSAERIVLPDSTIAL
DYILEKMNFVLKGLHVYPDATERTLNVTRGLIFSQKVLLWLIEKGGITREDAYLIVQENAMAVWADQSKNLRDLLKQDPR
CSSILKDSDLDEIFQIKPYLERIPLIFKRLGIID

Sequences:

>Translated_434_residues
MIDRYSHPEISAIWELENKFKIWTDIEIYACEARANRGEVPKEDLETIKQKAKFNVDEILEIESKVHHDVIAYLTNLNSY
IGPAGRHVHFGLTSSDVGDTALCVQMVQAMDLLIQRTETLLETTKQKAKEYKDLPCIGRSHGIHAEPMTLGLKFALFFAE
MTRNLERMKDAREQVAVGKLSGAVGTYSNIDLEIEEYVLNKLGLKVDPIATQVISRDRHAFYMSVLGVVAASLDRMATEI
RLLQKTEGREVEEPFAKGQKGSSAMPHKRNPVVCERISGISRVIRSNVNVGLQNVGLWHERDISHSSAERIVLPDSTIAL
DYILEKMNFVLKGLHVYPDATERTLNVTRGLIFSQKVLLWLIEKGGITREDAYLIVQENAMAVWADQSKNLRDLLKQDPR
CSSILKDSDLDEIFQIKPYLERIPLIFKRLGIID
>Mature_434_residues
MIDRYSHPEISAIWELENKFKIWTDIEIYACEARANRGEVPKEDLETIKQKAKFNVDEILEIESKVHHDVIAYLTNLNSY
IGPAGRHVHFGLTSSDVGDTALCVQMVQAMDLLIQRTETLLETTKQKAKEYKDLPCIGRSHGIHAEPMTLGLKFALFFAE
MTRNLERMKDAREQVAVGKLSGAVGTYSNIDLEIEEYVLNKLGLKVDPIATQVISRDRHAFYMSVLGVVAASLDRMATEI
RLLQKTEGREVEEPFAKGQKGSSAMPHKRNPVVCERISGISRVIRSNVNVGLQNVGLWHERDISHSSAERIVLPDSTIAL
DYILEKMNFVLKGLHVYPDATERTLNVTRGLIFSQKVLLWLIEKGGITREDAYLIVQENAMAVWADQSKNLRDLLKQDPR
CSSILKDSDLDEIFQIKPYLERIPLIFKRLGIID

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=438, Percent_Identity=28.5388127853881, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=29.3963254593176, Blast_Score=165, Evalue=8e-41,
Organism=Escherichia coli, GI1787376, Length=305, Percent_Identity=26.2295081967213, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787896, Length=212, Percent_Identity=29.7169811320755, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17508577, Length=416, Percent_Identity=25.7211538461538, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI32564234, Length=344, Percent_Identity=26.453488372093, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323391, Length=442, Percent_Identity=29.6380090497738, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24647570, Length=439, Percent_Identity=25.5125284738041, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI78710009, Length=221, Percent_Identity=30.316742081448, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640177, Length=223, Percent_Identity=30.0448430493274, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24640179, Length=223, Percent_Identity=30.0448430493274, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24662684, Length=221, Percent_Identity=30.316742081448, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49213; Mature: 49213

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRYSHPEISAIWELENKFKIWTDIEIYACEARANRGEVPKEDLETIKQKAKFNVDEIL
CCCCCCCCCHHEEEEECCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHH
EIESKVHHDVIAYLTNLNSYIGPAGRHVHFGLTSSDVGDTALCVQMVQAMDLLIQRTETL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LETTKQKAKEYKDLPCIGRSHGIHAEPMTLGLKFALFFAEMTRNLERMKDAREQVAVGKL
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYSNIDLEIEEYVLNKLGLKVDPIATQVISRDRHAFYMSVLGVVAASLDRMATEI
CCCCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RLLQKTEGREVEEPFAKGQKGSSAMPHKRNPVVCERISGISRVIRSNVNVGLQNVGLWHE
HHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCC
RDISHSSAERIVLPDSTIALDYILEKMNFVLKGLHVYPDATERTLNVTRGLIFSQKVLLW
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LIEKGGITREDAYLIVQENAMAVWADQSKNLRDLLKQDPRCSSILKDSDLDEIFQIKPYL
HHHCCCCCCCCCEEEEECCEEEEEECCCCCHHHHHHCCCCHHHHHCCCCHHHHHHHHHHH
ERIPLIFKRLGIID
HHHHHHHHHHCCCC
>Mature Secondary Structure
MIDRYSHPEISAIWELENKFKIWTDIEIYACEARANRGEVPKEDLETIKQKAKFNVDEIL
CCCCCCCCCHHEEEEECCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHH
EIESKVHHDVIAYLTNLNSYIGPAGRHVHFGLTSSDVGDTALCVQMVQAMDLLIQRTETL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LETTKQKAKEYKDLPCIGRSHGIHAEPMTLGLKFALFFAEMTRNLERMKDAREQVAVGKL
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYSNIDLEIEEYVLNKLGLKVDPIATQVISRDRHAFYMSVLGVVAASLDRMATEI
CCCCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RLLQKTEGREVEEPFAKGQKGSSAMPHKRNPVVCERISGISRVIRSNVNVGLQNVGLWHE
HHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCC
RDISHSSAERIVLPDSTIALDYILEKMNFVLKGLHVYPDATERTLNVTRGLIFSQKVLLW
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LIEKGGITREDAYLIVQENAMAVWADQSKNLRDLLKQDPRCSSILKDSDLDEIFQIKPYL
HHHCCCCCCCCCEEEEECCEEEEEECCCCCHHHHHHCCCCHHHHHCCCCHHHHHHHHHHH
ERIPLIFKRLGIID
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]