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The map label for this gene is dus1 [H]

Identifier: 183219434

GI number: 183219434

Start: 8817

End: 9830

Strand: Direct

Name: dus1 [H]

Synonym: LEPBI_I0007

Alternate gene names: 183219434

Gene position: 8817-9830 (Clockwise)

Preceding gene: 183219433

Following gene: 183219435

Centisome position: 0.24

GC content: 37.48

Gene sequence:

>1014_bases
ATGGTAAGGGTAGGAAATGTAATCATCGAAGGAGACGTTGCCTTGTCTCCGATGGCTGGAATTTCTGATAGCCCTTACCG
CCAGATCACTAAACGTTTTGGATCGGCTTTTTCTTATACTGAATTTGTTTCGACTGAACAATTAAAATTGGGAAATGCAA
AGTCACTGGATATGTTCCGATTTGTAGAAATGGAACGGCCCATTGTGTTTCAAATCTTTGGATCTGATTTGGATACTGTG
GTGAATGCCTCAGAGATTGCAGCAGAACAAAAACCAGACATCATTGATTTGAATATGGGCTGTTCTGTATCGAAAGTTTC
TCACAACGGATCTGGTGCAGGTTTACTCAAAAACATACGTTTGGCTGGTAAAATGATCGAAGGGATTCGGAACCGAACAA
ACCTTCCTGTCACAGCAAAGATTCGTTTGGGATGGGATGCGAATTCGTTGAATTACCTAGAAACTCTGAAGGTATTAGAA
GACTCTGGAGTATCCGCAATTTCTGTTCATGGAAGAACCAAAGCAATGGCATACACGGGTATTGCCGATTGGAACGCCAT
TGGTGAAATCAAATCTAGAGCGAATGTTCCCATCTACGGAAATGGCGATGTACAAAGCTACAAGGAAGCTTTAGAGAAAA
AGAAAACTTATGGTGTTGATTTAGTCCTAATTGGAAGGAAAGCAATTGGGAATCCCTGGGTATTTGGAAAAACTCCAAAA
CAATTTTTTCCTTGGTATCAAATCAAAGATGTCATTTTGGAACATTTGGATTTGATGTTGAATTTCTATCCAACTAGTGA
TGATTATGCGTTAATTTTATTTCGAAAACATTTTATCAAATATATAGAAAACATAGGATTTCCTGAAGATACAAAACGTT
TGTTACTTTCTGTGGCTTCAGTGAATGAATTTAAAGATACATTGGAATTTGTGAAAATGGATTCTACGATTTTGGAAACA
AGCGAATTAAACAACGAGAATATAAACTGTGAAACGTTTGTAAGTCTTGTATAA

Upstream 100 bases:

>100_bases
GTCAAAGTAGTGAATACGAAAGACGAAGACTTTGTGAAAGATTTTGCCGTCGTTCGAGAATCGGATTCGGACTCCGCTGA
GTCTTAATTAGGAAATCAAA

Downstream 100 bases:

>100_bases
GGAAACTGAAATGGCAGATTCAAAACAATCGATATTCTCTGATAGTTATAGCAAATATGGTGGAGCAAAACAAAAACGAA
AAGATGCTCGGGTGAAATTA

Product: putative tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MVRVGNVIIEGDVALSPMAGISDSPYRQITKRFGSAFSYTEFVSTEQLKLGNAKSLDMFRFVEMERPIVFQIFGSDLDTV
VNASEIAAEQKPDIIDLNMGCSVSKVSHNGSGAGLLKNIRLAGKMIEGIRNRTNLPVTAKIRLGWDANSLNYLETLKVLE
DSGVSAISVHGRTKAMAYTGIADWNAIGEIKSRANVPIYGNGDVQSYKEALEKKKTYGVDLVLIGRKAIGNPWVFGKTPK
QFFPWYQIKDVILEHLDLMLNFYPTSDDYALILFRKHFIKYIENIGFPEDTKRLLLSVASVNEFKDTLEFVKMDSTILET
SELNNENINCETFVSLV

Sequences:

>Translated_337_residues
MVRVGNVIIEGDVALSPMAGISDSPYRQITKRFGSAFSYTEFVSTEQLKLGNAKSLDMFRFVEMERPIVFQIFGSDLDTV
VNASEIAAEQKPDIIDLNMGCSVSKVSHNGSGAGLLKNIRLAGKMIEGIRNRTNLPVTAKIRLGWDANSLNYLETLKVLE
DSGVSAISVHGRTKAMAYTGIADWNAIGEIKSRANVPIYGNGDVQSYKEALEKKKTYGVDLVLIGRKAIGNPWVFGKTPK
QFFPWYQIKDVILEHLDLMLNFYPTSDDYALILFRKHFIKYIENIGFPEDTKRLLLSVASVNEFKDTLEFVKMDSTILET
SELNNENINCETFVSLV
>Mature_337_residues
MVRVGNVIIEGDVALSPMAGISDSPYRQITKRFGSAFSYTEFVSTEQLKLGNAKSLDMFRFVEMERPIVFQIFGSDLDTV
VNASEIAAEQKPDIIDLNMGCSVSKVSHNGSGAGLLKNIRLAGKMIEGIRNRTNLPVTAKIRLGWDANSLNYLETLKVLE
DSGVSAISVHGRTKAMAYTGIADWNAIGEIKSRANVPIYGNGDVQSYKEALEKKKTYGVDLVLIGRKAIGNPWVFGKTPK
QFFPWYQIKDVILEHLDLMLNFYPTSDDYALILFRKHFIKYIENIGFPEDTKRLLLSVASVNEFKDTLEFVKMDSTILET
SELNNENINCETFVSLV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=301, Percent_Identity=27.5747508305648, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI239788462, Length=290, Percent_Identity=27.9310344827586, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI40807366, Length=247, Percent_Identity=26.7206477732794, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI31742496, Length=302, Percent_Identity=25.8278145695364, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=27.3469387755102, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789660, Length=318, Percent_Identity=28.6163522012579, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1788462, Length=236, Percent_Identity=31.7796610169492, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693211, Length=253, Percent_Identity=24.901185770751, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17507177, Length=265, Percent_Identity=25.2830188679245, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=238, Percent_Identity=28.1512605042017, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI25144369, Length=286, Percent_Identity=24.4755244755245, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=262, Percent_Identity=27.4809160305344, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=304, Percent_Identity=23.0263157894737, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=25.6637168141593, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24585320, Length=229, Percent_Identity=30.1310043668122, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24580595, Length=274, Percent_Identity=24.0875912408759, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19920448, Length=274, Percent_Identity=24.0875912408759, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19921524, Length=225, Percent_Identity=24.8888888888889, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37659; Mature: 37659

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRVGNVIIEGDVALSPMAGISDSPYRQITKRFGSAFSYTEFVSTEQLKLGNAKSLDMFR
CEEECCEEEECCEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCEECCCCCCCHHHH
FVEMERPIVFQIFGSDLDTVVNASEIAAEQKPDIIDLNMGCSVSKVSHNGSGAGLLKNIR
HHHCCCCEEEEEECCCHHHHHCHHHHHHCCCCCEEEECCCCCEEEECCCCCCCHHHHHHH
LAGKMIEGIRNRTNLPVTAKIRLGWDANSLNYLETLKVLEDSGVSAISVHGRTKAMAYTG
HHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEEEC
IADWNAIGEIKSRANVPIYGNGDVQSYKEALEKKKTYGVDLVLIGRKAIGNPWVFGKTPK
CCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCEEEEECCHHCCCCEEECCCHH
QFFPWYQIKDVILEHLDLMLNFYPTSDDYALILFRKHFIKYIENIGFPEDTKRLLLSVAS
HHCCHHHHHHHHHHHHHHHEEEECCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VNEFKDTLEFVKMDSTILETSELNNENINCETFVSLV
HHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHEEEC
>Mature Secondary Structure
MVRVGNVIIEGDVALSPMAGISDSPYRQITKRFGSAFSYTEFVSTEQLKLGNAKSLDMFR
CEEECCEEEECCEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCEECCCCCCCHHHH
FVEMERPIVFQIFGSDLDTVVNASEIAAEQKPDIIDLNMGCSVSKVSHNGSGAGLLKNIR
HHHCCCCEEEEEECCCHHHHHCHHHHHHCCCCCEEEECCCCCEEEECCCCCCCHHHHHHH
LAGKMIEGIRNRTNLPVTAKIRLGWDANSLNYLETLKVLEDSGVSAISVHGRTKAMAYTG
HHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEEEC
IADWNAIGEIKSRANVPIYGNGDVQSYKEALEKKKTYGVDLVLIGRKAIGNPWVFGKTPK
CCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCEEEEECCHHCCCCEEECCCHH
QFFPWYQIKDVILEHLDLMLNFYPTSDDYALILFRKHFIKYIENIGFPEDTKRLLLSVAS
HHCCHHHHHHHHHHHHHHHEEEECCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VNEFKDTLEFVKMDSTILETSELNNENINCETFVSLV
HHHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]