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The map label for this gene is rpoA

Identifier: 18311358

GI number: 18311358

Start: 2737177

End: 2738124

Strand: Reverse

Name: rpoA

Synonym: CPE2376

Alternate gene names: 18311358

Gene position: 2738124-2737177 (Counterclockwise)

Preceding gene: 18311359

Following gene: 18311357

Centisome position: 90.32

GC content: 31.75

Gene sequence:

>948_bases
ATGTTAGAAATAGAAAAGCCAGTAATTCAATGTGTTGAATCAAACGATAACGGAACATATGGTAAGTTTGAGATAGAACC
ATTAGAAAGAGGTTATGGAATAACTCTTGGTAATGCATTAAGAAGAATATTACTTTCTTCTTTACCTGGAGTGGCTCCAA
CTTCAGTTAAAATAGATTCAGTTCTTCATGAGTTTTCAACAATAACAGGTGTTAAAGAAGATGTTACAGAGATAATCTTA
AATCTTAAAATGTTAGCACTTACTATGGAAGGTGAAGGACCAAAAACTATTTATATTGATGCTCAAGGACCAGGTGTTGT
TACTGGAGCAGATATAAAAACAGATGGTGATGTAGAAGTTGTAAACAAAGATCTACATATCGCTACATTAGACAACGATG
GTAAGTTATATATGGAAATCGTAGTTAATAGAGGAAGAGGATATGTTACTCAAAACAAAAATAAAACAGAAGATCTTCCA
TTATCAGCTATTGCTATAGATTCTATATACACTCCTGTAAAGAGAGTTAATTTCTCTGTTCAAAATACAAGAGTTGGTCA
AATTACTGACTATGATAAACTTACATTAGAAATTTGGACTAATGGAACTATAAGAATAGAAGAAGCGATTTCATTATCAG
CTAAAATTCTTATAGAGCACTTCAAATTATTCATGACTTTAACTGACAACGCTAATGATGTTGAGATAATGATTGAAAAA
GAAGAAGATAAAAAAGAAAAAGCTTTAGAGATGACTATAGAAGAGCTTGACTTATCAGTAAGATCATACAATTGCTTAAA
GAGAGCAGGTATAAACACTGTTCAAGAGCTTGCGGGCAAGAGCATGGATGATATGATGAAAGTTAGAAACTTAGGTAAGA
AATCTCTAGAAGAAGTAGAAAGAAAGTTAAACGAGTTAGGCTTAAATTTAAGACTAAATGACGAGTAG

Upstream 100 bases:

>100_bases
GTTATACAGCAAATAATAATTATTGATCTTAAGAGTACTTTTACAATTGGATCAGTTGCCCTCATAAGGTTGCCATTTTA
ATATTAAGGAGGGTTTTTAA

Downstream 100 bases:

>100_bases
GTGAGGAGGTAAAGCCATGGCAGGATATCGTAAGTTAGGTCGTCCTACTGATCAAAGAAAAGCTATGCTTAGAAATCTTG
TTACAAGCTTCTTAAAGCAT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MLEIEKPVIQCVESNDNGTYGKFEIEPLERGYGITLGNALRRILLSSLPGVAPTSVKIDSVLHEFSTITGVKEDVTEIIL
NLKMLALTMEGEGPKTIYIDAQGPGVVTGADIKTDGDVEVVNKDLHIATLDNDGKLYMEIVVNRGRGYVTQNKNKTEDLP
LSAIAIDSIYTPVKRVNFSVQNTRVGQITDYDKLTLEIWTNGTIRIEEAISLSAKILIEHFKLFMTLTDNANDVEIMIEK
EEDKKEKALEMTIEELDLSVRSYNCLKRAGINTVQELAGKSMDDMMKVRNLGKKSLEEVERKLNELGLNLRLNDE

Sequences:

>Translated_315_residues
MLEIEKPVIQCVESNDNGTYGKFEIEPLERGYGITLGNALRRILLSSLPGVAPTSVKIDSVLHEFSTITGVKEDVTEIIL
NLKMLALTMEGEGPKTIYIDAQGPGVVTGADIKTDGDVEVVNKDLHIATLDNDGKLYMEIVVNRGRGYVTQNKNKTEDLP
LSAIAIDSIYTPVKRVNFSVQNTRVGQITDYDKLTLEIWTNGTIRIEEAISLSAKILIEHFKLFMTLTDNANDVEIMIEK
EEDKKEKALEMTIEELDLSVRSYNCLKRAGINTVQELAGKSMDDMMKVRNLGKKSLEEVERKLNELGLNLRLNDE
>Mature_315_residues
MLEIEKPVIQCVESNDNGTYGKFEIEPLERGYGITLGNALRRILLSSLPGVAPTSVKIDSVLHEFSTITGVKEDVTEIIL
NLKMLALTMEGEGPKTIYIDAQGPGVVTGADIKTDGDVEVVNKDLHIATLDNDGKLYMEIVVNRGRGYVTQNKNKTEDLP
LSAIAIDSIYTPVKRVNFSVQNTRVGQITDYDKLTLEIWTNGTIRIEEAISLSAKILIEHFKLFMTLTDNANDVEIMIEK
EEDKKEKALEMTIEELDLSVRSYNCLKRAGINTVQELAGKSMDDMMKVRNLGKKSLEEVERKLNELGLNLRLNDE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=303, Percent_Identity=44.5544554455446, Blast_Score=234, Evalue=4e-63,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_CLOP1 (Q0TMS5)

Other databases:

- EMBL:   AB034247
- EMBL:   CP000246
- RefSeq:   YP_697065.1
- ProteinModelPortal:   Q0TMS5
- STRING:   Q0TMS5
- GeneID:   4201297
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2685
- TIGR:   CPF_2685
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   CPER195103:CPF_2685-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 35177; Mature: 35177

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIEKPVIQCVESNDNGTYGKFEIEPLERGYGITLGNALRRILLSSLPGVAPTSVKIDS
CCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCHHHH
VLHEFSTITGVKEDVTEIILNLKMLALTMEGEGPKTIYIDAQGPGVVTGADIKTDGDVEV
HHHHHHHHCCCHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCCEEECCCCCCCCCEEE
VNKDLHIATLDNDGKLYMEIVVNRGRGYVTQNKNKTEDLPLSAIAIDSIYTPVKRVNFSV
EECCEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCCCCCCEEEHHHHHCCHHHHEEEE
QNTRVGQITDYDKLTLEIWTNGTIRIEEAISLSAKILIEHFKLFMTLTDNANDVEIMIEK
CCCCCCCCCCCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEC
EEDKKEKALEMTIEELDLSVRSYNCLKRAGINTVQELAGKSMDDMMKVRNLGKKSLEEVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
RKLNELGLNLRLNDE
HHHHHCCCCEEECCC
>Mature Secondary Structure
MLEIEKPVIQCVESNDNGTYGKFEIEPLERGYGITLGNALRRILLSSLPGVAPTSVKIDS
CCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCHHHH
VLHEFSTITGVKEDVTEIILNLKMLALTMEGEGPKTIYIDAQGPGVVTGADIKTDGDVEV
HHHHHHHHCCCHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCCEEECCCCCCCCCEEE
VNKDLHIATLDNDGKLYMEIVVNRGRGYVTQNKNKTEDLPLSAIAIDSIYTPVKRVNFSV
EECCEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCCCCCCEEEHHHHHCCHHHHEEEE
QNTRVGQITDYDKLTLEIWTNGTIRIEEAISLSAKILIEHFKLFMTLTDNANDVEIMIEK
CCCCCCCCCCCCEEEEEEECCCEEEEHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEC
EEDKKEKALEMTIEELDLSVRSYNCLKRAGINTVQELAGKSMDDMMKVRNLGKKSLEEVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
RKLNELGLNLRLNDE
HHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA