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The map label for this gene is truA

Identifier: 18311353

GI number: 18311353

Start: 2732995

End: 2733729

Strand: Reverse

Name: truA

Synonym: CPE2371

Alternate gene names: 18311353

Gene position: 2733729-2732995 (Counterclockwise)

Preceding gene: 18311354

Following gene: 18311352

Centisome position: 90.18

GC content: 31.02

Gene sequence:

>735_bases
ATGAGGAATATAAAATTAACTATAGAATATGATGGAACATCTTATTTTGGGTGGCAAAAACAGCCTATAGGAAATACTAT
TCAGCAGAAGGTAGAAGAGGCTATAAAGAAGGTTACTAAAGAAGAGGTAGAAATACTAGGAAGTAGTAGAACAGATTCAG
GAGTCCATGCTAAGGCCTATGTGGCTAATTTTAAAACTAATTCTAATATACCAGGAAAAAATTTTAAGGCAGCATTAAAT
TCAAAATTACCAAAGGATATAGTAATAATAAATTCAGAAGAGGTAGCTGAGGATTTTCATGCTAGGTATATGACTACAGG
TAAAACTTATTGTTACACAATACTAAATAGAGAAGAGCCACCAGCATTAGAAAGAAATTATGTATATCATGTTAAAAAGC
AATTAGATGTTGAATCTATGAAAGAAGCATGTAAATACTTTTTAGGAAAACATGACTTTAAGGCCTTTCAAAGACCCGGT
GGGACAGTTAAGACCTCTGTAAGAACAATAACTGATATTCATATTGAAACAGAAGGAAATAAAATTAAAATATATGTATC
AGCAGATGGCTTTTTATATAATATGGTAAGATTAATTGTAGGAACATTACTTAAAGTAGGAAGAGGAAAAGAGAAGCCAG
AATATATAAAGGAAGTCATAGATTCTGGTGATAGAAAAAAAGCTGGAATTTGTGTTCCACCAACAGGATTATGCTTAGAA
AAAGTTTTTTACTAA

Upstream 100 bases:

>100_bases
TATAAAAGAAAGACTGCAAATTTGTAGTCTTTCTTTGTTTGAAGAATTAATTATTTATGATTTAAAATATTGTTTTATAA
AAAAATAAAGGAATGATTAT

Downstream 100 bases:

>100_bases
AAGTAGATGAAATGTATTGACACGCCCGTAAGCGTGTATTATAATTGTATTTGTTTGTAAGAGAGATGTACGGGATTCAA
TATGAATCTTAACATAAAGT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 2; tRNA-uridine isomerase I 2

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MRNIKLTIEYDGTSYFGWQKQPIGNTIQQKVEEAIKKVTKEEVEILGSSRTDSGVHAKAYVANFKTNSNIPGKNFKAALN
SKLPKDIVIINSEEVAEDFHARYMTTGKTYCYTILNREEPPALERNYVYHVKKQLDVESMKEACKYFLGKHDFKAFQRPG
GTVKTSVRTITDIHIETEGNKIKIYVSADGFLYNMVRLIVGTLLKVGRGKEKPEYIKEVIDSGDRKKAGICVPPTGLCLE
KVFY

Sequences:

>Translated_244_residues
MRNIKLTIEYDGTSYFGWQKQPIGNTIQQKVEEAIKKVTKEEVEILGSSRTDSGVHAKAYVANFKTNSNIPGKNFKAALN
SKLPKDIVIINSEEVAEDFHARYMTTGKTYCYTILNREEPPALERNYVYHVKKQLDVESMKEACKYFLGKHDFKAFQRPG
GTVKTSVRTITDIHIETEGNKIKIYVSADGFLYNMVRLIVGTLLKVGRGKEKPEYIKEVIDSGDRKKAGICVPPTGLCLE
KVFY
>Mature_244_residues
MRNIKLTIEYDGTSYFGWQKQPIGNTIQQKVEEAIKKVTKEEVEILGSSRTDSGVHAKAYVANFKTNSNIPGKNFKAALN
SKLPKDIVIINSEEVAEDFHARYMTTGKTYCYTILNREEPPALERNYVYHVKKQLDVESMKEACKYFLGKHDFKAFQRPG
GTVKTSVRTITDIHIETEGNKIKIYVSADGFLYNMVRLIVGTLLKVGRGKEKPEYIKEVIDSGDRKKAGICVPPTGLCLE
KVFY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=263, Percent_Identity=30.0380228136882, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI199559785, Length=258, Percent_Identity=25.968992248062, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788657, Length=241, Percent_Identity=34.0248962655602, Blast_Score=160, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI71985046, Length=263, Percent_Identity=25.8555133079848, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA2_CLOPE (Q8XHV5)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_563287.1
- ProteinModelPortal:   Q8XHV5
- SMR:   Q8XHV5
- GeneID:   990733
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE2371
- NMPDR:   fig|195102.1.peg.2434
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q8XHV5
- ProtClustDB:   PRK00021
- BioCyc:   CPER195102:CPE2371-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27656; Mature: 27656

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIKLTIEYDGTSYFGWQKQPIGNTIQQKVEEAIKKVTKEEVEILGSSRTDSGVHAKAY
CCCEEEEEEECCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VANFKTNSNIPGKNFKAALNSKLPKDIVIINSEEVAEDFHARYMTTGKTYCYTILNREEP
EEECCCCCCCCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCC
PALERNYVYHVKKQLDVESMKEACKYFLGKHDFKAFQRPGGTVKTSVRTITDIHIETEGN
CCCCCCHHEEHHHHCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEHEEEEEEEEEEEECCC
KIKIYVSADGFLYNMVRLIVGTLLKVGRGKEKPEYIKEVIDSGDRKKAGICVPPTGLCLE
EEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEECCCCHHHHH
KVFY
HHCC
>Mature Secondary Structure
MRNIKLTIEYDGTSYFGWQKQPIGNTIQQKVEEAIKKVTKEEVEILGSSRTDSGVHAKAY
CCCEEEEEEECCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VANFKTNSNIPGKNFKAALNSKLPKDIVIINSEEVAEDFHARYMTTGKTYCYTILNREEP
EEECCCCCCCCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCC
PALERNYVYHVKKQLDVESMKEACKYFLGKHDFKAFQRPGGTVKTSVRTITDIHIETEGN
CCCCCCHHEEHHHHCCHHHHHHHHHHHHCCHHHHHHHCCCCCEEHEEEEEEEEEEEECCC
KIKIYVSADGFLYNMVRLIVGTLLKVGRGKEKPEYIKEVIDSGDRKKAGICVPPTGLCLE
EEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEECCCCHHHHH
KVFY
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842