Click here to switch to the map view.

The map label for this gene is hydA [C]

Identifier: 18311328

GI number: 18311328

Start: 2703382

End: 2705100

Strand: Reverse

Name: hydA [C]

Synonym: CPE2346

Alternate gene names: 18311328

Gene position: 2705100-2703382 (Counterclockwise)

Preceding gene: 18311329

Following gene: 18311327

Centisome position: 89.24

GC content: 33.51

Gene sequence:

>1719_bases
ATGAATAAAATAATAATCAATGATAAGACTATCGAATTTGATGGTGACAAGACGATACTTGACTTAGCAAGAGAGAATGG
ATTTGACATTCCAGTGCTTTGTGAGTTAAAAAATTGCGGAAACAAAGGACAATGTGGAGTTTGTCTTGTTGAGCAAGAAG
GAAATGATAGACTATTAAGATCTTGTGCAATAAAAGCTAAAGATGGAATGGTTATTAAAACTGATAGTGAAAAAGTACTA
GAGGCTAGAAAAGAAAGAGTTGCAGAACTTTTAGATGAGCATGAGTTCAAATGTGGACCATGTAAAAGAAGAGAGAATTG
TGAATTCTTAAAACTTGTAATTAAAACAAAGGCAAGAGCTCACAAACCATTTGTAGTTGCAGATAAATCAGAATATGTAG
ATGACAGAAGTAAATCAATAGTTTTAGATAGAAGTAAATGTGTTAAATGTGGTAGATGTGTTGCAGCATGTAGAACAAGA
ACTGCTACTAACTCAATAAAATTCCACAGAATTGATGGAGTAAGATTAGTAGGACCAGAAGAATTAAAATGTTTCGACGA
TACAAATTGTTTATTATGTGGACAATGTATAGCTGCTTGCCCAGTTGATGCATTATCAGAAAAATCACATATTGAAAGAG
TTCAAGACGCATTAAATGATCCAGAAAAGCATGTAATAGTTGCTATGGCACCAGCTGTAAGAACATCAATGGGTGAATTA
TTCAAGATGGGTTATGGACAAGATGTTACTGGAAAATTATATACTGCTTTAAGAGAATTAGGTTTTGATAAAGTATTTGA
TATAAACTTCGGTGCTGATATGACGATAATGGAAGAAGCTACTGAGCTTATAGAAAGAATAAAAAATAACGGACCTTTCC
CAATGTTAACTTCATGTTGTCCATCATGGGTTAGAGAAGTTGAAAACTACTTCCCAGAATTAGTAGAAAATCTTTCATCA
GCTAAATCACCACAACAAATATTTGGTGCAGCATCAAAAACTTATTATCCACAAGTTGCTGATATAGATCCTAAAAAAGT
ATTTACAGTAACTGTAATGCCTTGTACTTCTAAAAAATTCGAGGCTGATAGACCTGAAATGGAAAATGAAGGAATAAGAA
ACATAGATGCAGTTATAACAACTAGAGAGTTAGCTAGAATGATAAAAGCTGCTAAAATAGATTTTGCTAAATTAGAAGAT
GGTGAAGTGGATCCAGCTATGGGTGAGTACACTGGAGCAGGTGTTATATTTGGAGCTACTGGTGGAGTTATGGAAGCTGC
TTTAAGAACAGCTAAAGATTTCATGGAAAATGACAACTTAGATAATGTAGATTATGAAGCTGTTAGAGGATTAGCTGGAA
TAAAAGAAGCTGAAGTAGAAATAGCAGGAAATGAATATAAATTAGCGGTTGTAAGTGGAGCTGCTAATGTATTTGAACTA
GTTAAGTCAGGTAAAATAAATGACTATCACTTCATCGAAGTAATGGCATGTCCAGGTGGATGTGTTAACGGTGGAGGACA
ACCACACATCTCAGCTGAAGATAGTGATAAAATAGATATTAGAGAAGTAAGAGCTTCTGTTCTTTACAATCAAGATAAGA
ATTTAGAGAAGAGAAAATCACATCAAAACTCAGCTTTATTAAAAATGTATGAAAACTACATGGGTAAACCAGGTCATGGA
AGAGCTCATGAGTTATTACACATGAAATATAAAAAATAA

Upstream 100 bases:

>100_bases
TATGTTAGAATAATAACGACGATTTTTAAATTAACTGATTTTTACAATGTATGAGTTGTGGTTTAGTATTAAGTAAAAAA
CCAAAAATTGGAGGAACAGC

Downstream 100 bases:

>100_bases
TATTTAAAATATAGACAGGATGTTTAATGTATCCTGTCTTTTATATTTAAAAATATCACAATAAAAATAAAATTTATACA
CTCTATTTAGATTATCATAC

Product: iron hydrogenase

Products: NA

Alternate protein names: CpI; Fe-only hydrogenase; [Fe] hydrogenase [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MNKIIINDKTIEFDGDKTILDLARENGFDIPVLCELKNCGNKGQCGVCLVEQEGNDRLLRSCAIKAKDGMVIKTDSEKVL
EARKERVAELLDEHEFKCGPCKRRENCEFLKLVIKTKARAHKPFVVADKSEYVDDRSKSIVLDRSKCVKCGRCVAACRTR
TATNSIKFHRIDGVRLVGPEELKCFDDTNCLLCGQCIAACPVDALSEKSHIERVQDALNDPEKHVIVAMAPAVRTSMGEL
FKMGYGQDVTGKLYTALRELGFDKVFDINFGADMTIMEEATELIERIKNNGPFPMLTSCCPSWVREVENYFPELVENLSS
AKSPQQIFGAASKTYYPQVADIDPKKVFTVTVMPCTSKKFEADRPEMENEGIRNIDAVITTRELARMIKAAKIDFAKLED
GEVDPAMGEYTGAGVIFGATGGVMEAALRTAKDFMENDNLDNVDYEAVRGLAGIKEAEVEIAGNEYKLAVVSGAANVFEL
VKSGKINDYHFIEVMACPGGCVNGGGQPHISAEDSDKIDIREVRASVLYNQDKNLEKRKSHQNSALLKMYENYMGKPGHG
RAHELLHMKYKK

Sequences:

>Translated_572_residues
MNKIIINDKTIEFDGDKTILDLARENGFDIPVLCELKNCGNKGQCGVCLVEQEGNDRLLRSCAIKAKDGMVIKTDSEKVL
EARKERVAELLDEHEFKCGPCKRRENCEFLKLVIKTKARAHKPFVVADKSEYVDDRSKSIVLDRSKCVKCGRCVAACRTR
TATNSIKFHRIDGVRLVGPEELKCFDDTNCLLCGQCIAACPVDALSEKSHIERVQDALNDPEKHVIVAMAPAVRTSMGEL
FKMGYGQDVTGKLYTALRELGFDKVFDINFGADMTIMEEATELIERIKNNGPFPMLTSCCPSWVREVENYFPELVENLSS
AKSPQQIFGAASKTYYPQVADIDPKKVFTVTVMPCTSKKFEADRPEMENEGIRNIDAVITTRELARMIKAAKIDFAKLED
GEVDPAMGEYTGAGVIFGATGGVMEAALRTAKDFMENDNLDNVDYEAVRGLAGIKEAEVEIAGNEYKLAVVSGAANVFEL
VKSGKINDYHFIEVMACPGGCVNGGGQPHISAEDSDKIDIREVRASVLYNQDKNLEKRKSHQNSALLKMYENYMGKPGHG
RAHELLHMKYKK
>Mature_572_residues
MNKIIINDKTIEFDGDKTILDLARENGFDIPVLCELKNCGNKGQCGVCLVEQEGNDRLLRSCAIKAKDGMVIKTDSEKVL
EARKERVAELLDEHEFKCGPCKRRENCEFLKLVIKTKARAHKPFVVADKSEYVDDRSKSIVLDRSKCVKCGRCVAACRTR
TATNSIKFHRIDGVRLVGPEELKCFDDTNCLLCGQCIAACPVDALSEKSHIERVQDALNDPEKHVIVAMAPAVRTSMGEL
FKMGYGQDVTGKLYTALRELGFDKVFDINFGADMTIMEEATELIERIKNNGPFPMLTSCCPSWVREVENYFPELVENLSS
AKSPQQIFGAASKTYYPQVADIDPKKVFTVTVMPCTSKKFEADRPEMENEGIRNIDAVITTRELARMIKAAKIDFAKLED
GEVDPAMGEYTGAGVIFGATGGVMEAALRTAKDFMENDNLDNVDYEAVRGLAGIKEAEVEIAGNEYKLAVVSGAANVFEL
VKSGKINDYHFIEVMACPGGCVNGGGQPHISAEDSDKIDIREVRASVLYNQDKNLEKRKSHQNSALLKMYENYMGKPGHG
RAHELLHMKYKK

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI11968051, Length=341, Percent_Identity=30.4985337243402, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI6912524, Length=405, Percent_Identity=28.641975308642, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI134284357, Length=368, Percent_Identity=28.5326086956522, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI84452151, Length=368, Percent_Identity=28.5326086956522, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI14165461, Length=448, Percent_Identity=27.2321428571429, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI145693161, Length=219, Percent_Identity=25.5707762557078, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71995015, Length=355, Percent_Identity=30.1408450704225, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17565758, Length=213, Percent_Identity=26.7605633802817, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566231, Length=213, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193209088, Length=212, Percent_Identity=26.8867924528302, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324089, Length=378, Percent_Identity=26.1904761904762, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI116007464, Length=406, Percent_Identity=25.615763546798, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI116007468, Length=406, Percent_Identity=25.615763546798, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI116007470, Length=406, Percent_Identity=25.615763546798, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI116007466, Length=406, Percent_Identity=25.615763546798, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI116007462, Length=290, Percent_Identity=27.5862068965517, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR003149
- InterPro:   IPR013352
- InterPro:   IPR001041 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF02256 Fe_hyd_SSU [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 63535; Mature: 63535

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS51085 2FE2S_FER_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIIINDKTIEFDGDKTILDLARENGFDIPVLCELKNCGNKGQCGVCLVEQEGNDRLLR
CCEEEECCEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEECCCHHHHH
SCAIKAKDGMVIKTDSEKVLEARKERVAELLDEHEFKCGPCKRRENCEFLKLVIKTKARA
HHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
HKPFVVADKSEYVDDRSKSIVLDRSKCVKCGRCVAACRTRTATNSIKFHRIDGVRLVGPE
CCCEEEECCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECHH
ELKCFDDTNCLLCGQCIAACPVDALSEKSHIERVQDALNDPEKHVIVAMAPAVRTSMGEL
HCEEECCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
FKMGYGQDVTGKLYTALRELGFDKVFDINFGADMTIMEEATELIERIKNNGPFPMLTSCC
HHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
PSWVREVENYFPELVENLSSAKSPQQIFGAASKTYYPQVADIDPKKVFTVTVMPCTSKKF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCC
EADRPEMENEGIRNIDAVITTRELARMIKAAKIDFAKLEDGEVDPAMGEYTGAGVIFGAT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEEECC
GGVMEAALRTAKDFMENDNLDNVDYEAVRGLAGIKEAEVEIAGNEYKLAVVSGAANVFEL
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEECCCEEEEEEEECHHHHHHH
VKSGKINDYHFIEVMACPGGCVNGGGQPHISAEDSDKIDIREVRASVLYNQDKNLEKRKS
HHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
HQNSALLKMYENYMGKPGHGRAHELLHMKYKK
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MNKIIINDKTIEFDGDKTILDLARENGFDIPVLCELKNCGNKGQCGVCLVEQEGNDRLLR
CCEEEECCEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEECCCHHHHH
SCAIKAKDGMVIKTDSEKVLEARKERVAELLDEHEFKCGPCKRRENCEFLKLVIKTKARA
HHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
HKPFVVADKSEYVDDRSKSIVLDRSKCVKCGRCVAACRTRTATNSIKFHRIDGVRLVGPE
CCCEEEECCHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECHH
ELKCFDDTNCLLCGQCIAACPVDALSEKSHIERVQDALNDPEKHVIVAMAPAVRTSMGEL
HCEEECCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
FKMGYGQDVTGKLYTALRELGFDKVFDINFGADMTIMEEATELIERIKNNGPFPMLTSCC
HHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
PSWVREVENYFPELVENLSSAKSPQQIFGAASKTYYPQVADIDPKKVFTVTVMPCTSKKF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCC
EADRPEMENEGIRNIDAVITTRELARMIKAAKIDFAKLEDGEVDPAMGEYTGAGVIFGAT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEEECC
GGVMEAALRTAKDFMENDNLDNVDYEAVRGLAGIKEAEVEIAGNEYKLAVVSGAANVFEL
CHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEECCCEEEEEEEECHHHHHHH
VKSGKINDYHFIEVMACPGGCVNGGGQPHISAEDSDKIDIREVRASVLYNQDKNLEKRKS
HHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
HQNSALLKMYENYMGKPGHGRAHELLHMKYKK
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1911757; 9836629; 10529166 [H]