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The map label for this gene is prfA

Identifier: 18311184

GI number: 18311184

Start: 2521090

End: 2522172

Strand: Reverse

Name: prfA

Synonym: CPE2202

Alternate gene names: 18311184

Gene position: 2522172-2521090 (Counterclockwise)

Preceding gene: 18311185

Following gene: 18311183

Centisome position: 83.2

GC content: 33.24

Gene sequence:

>1083_bases
ATGATACTTGATAGATTAAATTTTATTGAAAATAAATATGATGAACTTTCAGTTAAGATTAGTGATCCTTCAATAATGGC
TAACCAAAAAGAGTGGAGAAAGCTTTGTAAGGAGCACGCTGACTTAGAAGTTATAGTTAATAAGTACAAAGAGTACAAAG
AAGCGACAGAAGAATTAGAAGCTAACAAAGAGATGCTTTCAGAAGAAAGTGATCAAGAAATGAGAGAAATGATAAACTCT
GAAATCAAGGACTTAACAGAAAGAAAAAAAGAATTAGAGGACGAAATACAAATTCTTTTACTACCAAAAGATCCTAATGA
TGATAAAAACGTATTTGTTGAAATCAGAGGTGGTGCTGGTGGAGATGAAGCAGCACTATTTGCAGCTAATCTTTTCAGAA
TGTACACTAAATACGCTGAAAAGAATAGATGGAAAGTTGAACTAATGAGTGCAAATGAAACTGACATTGGAGGTTTCAAA
GAGGTTGTATTCATGATAAAAGGAGCAGGTGCTTATTCAAAACTTAAATATGAGAGTGGAGCACACAGAGTTCAAAGGGT
TCCTGATACAGAAAGTTCAGGAAGAATACATACTTCAACTGCTACAGTAGCTGTTTTACCAGAAGTTGATGATGTAGAAA
TCGAAATAAACGATAAAGATATTAAAATAGACGTATTTAGAGCATCAGGTAATGGTGGTCAGTGCGTTAACACAACTGAT
TCAGCTGTAAGAATAACTCACTTACCTTCAGGATTAGTTGTTTCATGTCAGGATGAAAAATCACAGCTTAAAAATAAAGA
AAAAGCTATGAAAGTATTAAGAGCAAGATTATTCGAGCAAGCTGAAGCTGAAAGATTAGCTGGTATTGCTGAAGATAGAA
AGAGTCAGGTTGGTACTGGAGATAGAAGTGAAAGAATAAGAACTTACAACTATCCACAAGGAAGAGTAACTGATCACAGA
ATAAACATGACTTTATATAAATTAGATAGTTTCTTAGAAGGTGACATAGACGAAATATTAAATGCTCTAATTACTGAAGA
TCAAGCTCAAAAGATGAAAGCAATGGGTAATACTGAATTTTAA

Upstream 100 bases:

>100_bases
GTTTAGATAGATGTGTTATAGGAAGAGTAAGCCTTGAAAGATAGCATAGTAGTGTGCTATAATTGTAAAATATGAAAATC
TGTATACGGAGTGATAATAG

Downstream 100 bases:

>100_bases
TATACAAAGCCTTACATGAATAATGTAAGGCTTTAAAAATACATAGTTTTAAAATGGTAATTAAAGAGGAATATAATTAT
GGATATAGATAAAGCTATAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MILDRLNFIENKYDELSVKISDPSIMANQKEWRKLCKEHADLEVIVNKYKEYKEATEELEANKEMLSEESDQEMREMINS
EIKDLTERKKELEDEIQILLLPKDPNDDKNVFVEIRGGAGGDEAALFAANLFRMYTKYAEKNRWKVELMSANETDIGGFK
EVVFMIKGAGAYSKLKYESGAHRVQRVPDTESSGRIHTSTATVAVLPEVDDVEIEINDKDIKIDVFRASGNGGQCVNTTD
SAVRITHLPSGLVVSCQDEKSQLKNKEKAMKVLRARLFEQAEAERLAGIAEDRKSQVGTGDRSERIRTYNYPQGRVTDHR
INMTLYKLDSFLEGDIDEILNALITEDQAQKMKAMGNTEF

Sequences:

>Translated_360_residues
MILDRLNFIENKYDELSVKISDPSIMANQKEWRKLCKEHADLEVIVNKYKEYKEATEELEANKEMLSEESDQEMREMINS
EIKDLTERKKELEDEIQILLLPKDPNDDKNVFVEIRGGAGGDEAALFAANLFRMYTKYAEKNRWKVELMSANETDIGGFK
EVVFMIKGAGAYSKLKYESGAHRVQRVPDTESSGRIHTSTATVAVLPEVDDVEIEINDKDIKIDVFRASGNGGQCVNTTD
SAVRITHLPSGLVVSCQDEKSQLKNKEKAMKVLRARLFEQAEAERLAGIAEDRKSQVGTGDRSERIRTYNYPQGRVTDHR
INMTLYKLDSFLEGDIDEILNALITEDQAQKMKAMGNTEF
>Mature_360_residues
MILDRLNFIENKYDELSVKISDPSIMANQKEWRKLCKEHADLEVIVNKYKEYKEATEELEANKEMLSEESDQEMREMINS
EIKDLTERKKELEDEIQILLLPKDPNDDKNVFVEIRGGAGGDEAALFAANLFRMYTKYAEKNRWKVELMSANETDIGGFK
EVVFMIKGAGAYSKLKYESGAHRVQRVPDTESSGRIHTSTATVAVLPEVDDVEIEINDKDIKIDVFRASGNGGQCVNTTD
SAVRITHLPSGLVVSCQDEKSQLKNKEKAMKVLRARLFEQAEAERLAGIAEDRKSQVGTGDRSERIRTYNYPQGRVTDHR
INMTLYKLDSFLEGDIDEILNALITEDQAQKMKAMGNTEF

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=46.2328767123288, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI34577120, Length=330, Percent_Identity=41.2121212121212, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI166795305, Length=201, Percent_Identity=42.7860696517413, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=50.974930362117, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=35.7142857142857, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=285, Percent_Identity=38.9473684210526, Blast_Score=194, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=43.4650455927052, Blast_Score=265, Evalue=6e-72,
Organism=Drosophila melanogaster, GI19921226, Length=340, Percent_Identity=42.0588235294118, Blast_Score=250, Evalue=9e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_CLOP1 (Q0TNB0)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_696880.1
- ProteinModelPortal:   Q0TNB0
- SMR:   Q0TNB0
- STRING:   Q0TNB0
- GeneID:   4202688
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2466
- TIGR:   CPF_2466
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   CPER195103:CPF_2466-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40947; Mature: 40947

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDRLNFIENKYDELSVKISDPSIMANQKEWRKLCKEHADLEVIVNKYKEYKEATEELE
CCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ANKEMLSEESDQEMREMINSEIKDLTERKKELEDEIQILLLPKDPNDDKNVFVEIRGGAG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEECCCC
GDEAALFAANLFRMYTKYAEKNRWKVELMSANETDIGGFKEVVFMIKGAGAYSKLKYESG
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCHHHEECCCC
AHRVQRVPDTESSGRIHTSTATVAVLPEVDDVEIEINDKDIKIDVFRASGNGGQCVNTTD
HHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCEEEEEEEEECCCCCCEECCCC
SAVRITHLPSGLVVSCQDEKSQLKNKEKAMKVLRARLFEQAEAERLAGIAEDRKSQVGTG
CEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNYPQGRVTDHRINMTLYKLDSFLEGDIDEILNALITEDQAQKMKAMGNTEF
CCCCCCEECCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MILDRLNFIENKYDELSVKISDPSIMANQKEWRKLCKEHADLEVIVNKYKEYKEATEELE
CCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ANKEMLSEESDQEMREMINSEIKDLTERKKELEDEIQILLLPKDPNDDKNVFVEIRGGAG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEECCCC
GDEAALFAANLFRMYTKYAEKNRWKVELMSANETDIGGFKEVVFMIKGAGAYSKLKYESG
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCHHHEECCCC
AHRVQRVPDTESSGRIHTSTATVAVLPEVDDVEIEINDKDIKIDVFRASGNGGQCVNTTD
HHHHHCCCCCCCCCEEEECCEEEEEECCCCCEEEEECCCEEEEEEEEECCCCCCEECCCC
SAVRITHLPSGLVVSCQDEKSQLKNKEKAMKVLRARLFEQAEAERLAGIAEDRKSQVGTG
CEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNYPQGRVTDHRINMTLYKLDSFLEGDIDEILNALITEDQAQKMKAMGNTEF
CCCCCCEECCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA