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The map label for this gene is glpF [H]

Identifier: 18309908

GI number: 18309908

Start: 1124547

End: 1125251

Strand: Direct

Name: glpF [H]

Synonym: CPE0926

Alternate gene names: 18309908

Gene position: 1124547-1125251 (Clockwise)

Preceding gene: 18309907

Following gene: 18309909

Centisome position: 37.1

GC content: 32.2

Gene sequence:

>705_bases
ATGGCATCATTATTAGCAGAATTAATAGGAACAATGCTACTTATCATACTTGGTGATGGTGTTGTTGCAAACGTAGTTTT
AAAGAAAACAAAGGGAAATAGCTCAGGATGGATAGTTATAACTACTGGATGGGCTTTAGCAGTTGCTATACCAGCAGCTA
TTTTTACTAGTGTGAGTGGAGCATTATTTAATCCAGCATTAACAATAGCATTAGCTATAGTTGGCCAATTTACATGGTCT
CAAGTTATTCCTTATATAATTGCACAACTTTTAGGAGCTTTTTTAGGAGCAGTTGTTGTTTATATAACTTACTATAATCA
TTTTGAAGAAACAGAAGATCAAAATTCAAAATTAGGAGTATTTTGTACTATTCCAGAAATAGAAGATCATAAAATAAACT
TCTTAACAGAGTTTATAGGAACTTTTGTTTTAACTTTTGCTGTATTAGGAATAGGAGCACAAAATATAGATTATGGTGTT
AAAGTAATTATAATAGGATGCTTAATATGGGCTATAGGATTAAGCTTAGGTGGTCCAACAGGATATGCAATAAATCCAGC
TAGAGATTTTGGTCCAAGATTAGCACACTTCTTACTTCCGATTCCTAGAAAAGGAAGTTCTAAATGGGAATATGCTTGGA
TTCCTATAGTTGCACCAATAATAGGAGCAATATGTGGAGCCTTAGTTTATGTAAATATATTTTAA

Upstream 100 bases:

>100_bases
TTTTAACATGTGTATTATAGTATAATTGTTGTTAAAAGAATATTAAATGTTTATTAAAAAGAATTGTATTTGTGTCATAA
ATATAGAGGGAGTATAATTT

Downstream 100 bases:

>100_bases
TAAACCAAGGTAAATAAGTTTAAAATTATTATCTATAGATCAATATAGATAATAATTTTTTTTAATTATAAATGATAAAT
TTCTTATAACTTTAAAAATA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MASLLAELIGTMLLIILGDGVVANVVLKKTKGNSSGWIVITTGWALAVAIPAAIFTSVSGALFNPALTIALAIVGQFTWS
QVIPYIIAQLLGAFLGAVVVYITYYNHFEETEDQNSKLGVFCTIPEIEDHKINFLTEFIGTFVLTFAVLGIGAQNIDYGV
KVIIIGCLIWAIGLSLGGPTGYAINPARDFGPRLAHFLLPIPRKGSSKWEYAWIPIVAPIIGAICGALVYVNIF

Sequences:

>Translated_234_residues
MASLLAELIGTMLLIILGDGVVANVVLKKTKGNSSGWIVITTGWALAVAIPAAIFTSVSGALFNPALTIALAIVGQFTWS
QVIPYIIAQLLGAFLGAVVVYITYYNHFEETEDQNSKLGVFCTIPEIEDHKINFLTEFIGTFVLTFAVLGIGAQNIDYGV
KVIIIGCLIWAIGLSLGGPTGYAINPARDFGPRLAHFLLPIPRKGSSKWEYAWIPIVAPIIGAICGALVYVNIF
>Mature_233_residues
ASLLAELIGTMLLIILGDGVVANVVLKKTKGNSSGWIVITTGWALAVAIPAAIFTSVSGALFNPALTIALAIVGQFTWSQ
VIPYIIAQLLGAFLGAVVVYITYYNHFEETEDQNSKLGVFCTIPEIEDHKINFLTEFIGTFVLTFAVLGIGAQNIDYGVK
VIIIGCLIWAIGLSLGGPTGYAINPARDFGPRLAHFLLPIPRKGSSKWEYAWIPIVAPIIGAICGALVYVNIF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=245, Percent_Identity=37.5510204081633, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI157266307, Length=242, Percent_Identity=35.9504132231405, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI22538420, Length=242, Percent_Identity=33.4710743801653, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4502187, Length=243, Percent_Identity=32.5102880658436, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI310133356, Length=221, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI310114181, Length=221, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1790362, Length=249, Percent_Identity=37.7510040160643, Blast_Score=123, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71994009, Length=240, Percent_Identity=33.75, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531431, Length=240, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531429, Length=240, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71992966, Length=247, Percent_Identity=34.412955465587, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17544068, Length=242, Percent_Identity=34.7107438016529, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71992961, Length=242, Percent_Identity=31.404958677686, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32564052, Length=239, Percent_Identity=30.5439330543933, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533613, Length=239, Percent_Identity=30.5439330543933, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558372, Length=233, Percent_Identity=28.755364806867, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321054, Length=204, Percent_Identity=32.3529411764706, Blast_Score=98, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6322985, Length=280, Percent_Identity=30.3571428571429, Blast_Score=89, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24652747, Length=234, Percent_Identity=31.6239316239316, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551084, Length=234, Percent_Identity=31.6239316239316, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25041; Mature: 24910

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLLAELIGTMLLIILGDGVVANVVLKKTKGNSSGWIVITTGWALAVAIPAAIFTSVSG
CHHHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHCH
ALFNPALTIALAIVGQFTWSQVIPYIIAQLLGAFLGAVVVYITYYNHFEETEDQNSKLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
FCTIPEIEDHKINFLTEFIGTFVLTFAVLGIGAQNIDYGVKVIIIGCLIWAIGLSLGGPT
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
GYAINPARDFGPRLAHFLLPIPRKGSSKWEYAWIPIVAPIIGAICGALVYVNIF
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASLLAELIGTMLLIILGDGVVANVVLKKTKGNSSGWIVITTGWALAVAIPAAIFTSVSG
HHHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHCH
ALFNPALTIALAIVGQFTWSQVIPYIIAQLLGAFLGAVVVYITYYNHFEETEDQNSKLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
FCTIPEIEDHKINFLTEFIGTFVLTFAVLGIGAQNIDYGVKVIIIGCLIWAIGLSLGGPT
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
GYAINPARDFGPRLAHFLLPIPRKGSSKWEYAWIPIVAPIIGAICGALVYVNIF
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]