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The map label for this gene is tufA [H]

Identifier: 182682613

GI number: 182682613

Start: 2367003

End: 2368193

Strand: Reverse

Name: tufA [H]

Synonym: XfasM23_2101

Alternate gene names: 182682613

Gene position: 2368193-2367003 (Counterclockwise)

Preceding gene: 182682614

Following gene: 182682612

Centisome position: 93.39

GC content: 49.87

Gene sequence:

>1191_bases
ATGGCTCAGGATAAGTTCAAACGCACTAAGTTGCACGTAAATGTTGGTACGATTGGTCACGTTGACCATGGCAAGACTAC
ACTGACTGCGGCGCTGACCAAGGTGGGTGCTGAGCGTTTTGGTGGCGAGTTTAAGGCATACGATGCGATTGACGCGGCAC
CTGAAGAGAAGGCGCGCGGGATTACTATTTCTACGGCGCACGTCGAGTATGAAACTGAAGTCCGCCACTATGCCCATGTG
GACTGTCCTGGTCATGCTGATTATGTGAAAAACATGATTACAGGTGCGGCGCAGATGGATGGTGCGATTTTAGTATGTTC
TGCTGCTGATGGTCCGATGCCTCAGACTCGTGAACACATTTTGTTGGCTCGCCAGGTGGGTGTGCCTTACATTGTTGTGT
TCTTGAATAAGGCTGACATGGTGGATGATGCTGAGTTGCTTGAGCTTGTGGAGATGGAAGTTCGTGAGTTGTTGAGCAAG
TATGATTTTCCTGGTGATGACACTCCTATTGTGAGAGGTTCCGCGTTGAAGGCGCTGGAGGGTGATCAGTCTGAGATTGG
TGTACCGGCGATTATTCGACTGGCGGAAGCATTAGATACCCACATTCCGAATCCGGAGCGTGCGATTGATCGTCCGTTTT
TGATGCCTGTGGAAGATGTGTTTTCGATTTCTGGTCGTGGCACTGTGGTGACAGGTCGTATTGAATGTGGTGTGATTAAG
GTTGGCGATGAGGTGGAAATAGTTGGCATTCGTCCGACGTCGAAGACGATTGTTACGGGTGTTGAGATGTTCCGTAAGTT
ATTGGATCAAGGTCAGGCTGGTGACAACGCGGGTCTATTGTTGCGCGGTACGAAGCGTGATGAAGTGGAGCGTGGTCAGG
TGTTGGCGAAGCCTGGTTGTATAAAGGCGCATAAAGAATTTGAGGCTGAGGTGTATGTGCTTTCGAAAGAGGAGGGTGGT
CGTCATACTCCGTTTTTCAATGGATACACTCCGCAGTTTTATATGCGTACAACAGACATTACCGGTAAGGTCTGTTTACC
TGAGGGTGTAGAGATGGTGATGCCTGGTGATAATGTGAAGGTGACGGTATCTCTGATTAATCCTGTGGCGATGGGTGAGG
GTCAGCGCTTTGCGATCCGTGAAGGTGGGCGGACAGTGGGTGCTGGCGTTGTTTCGAAGGTTATTGGTTAA

Upstream 100 bases:

>100_bases
GCGTTTGCCAGCTTGCCGAAGGCCATAAACAACGGCTCTTTTGCTGGGCGCTGTGTTATTAAAATCGAGTAAAAATTTAG
CAGAAGAGGTTTCTAATTCT

Downstream 100 bases:

>100_bases
CTAGAGTGCTTTGTAGTCCGGCGGTCTTAGCTCGTTGGATTTTTTCAAACGGCGGACCGATTTAATCTCGGTTCGCCGTT
GTTATTTCAAAAATTGTAAT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAQDKFKRTKLHVNVGTIGHVDHGKTTLTAALTKVGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYETEVRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDAELLELVEMEVRELLSK
YDFPGDDTPIVRGSALKALEGDQSEIGVPAIIRLAEALDTHIPNPERAIDRPFLMPVEDVFSISGRGTVVTGRIECGVIK
VGDEVEIVGIRPTSKTIVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDEVERGQVLAKPGCIKAHKEFEAEVYVLSKEEGG
RHTPFFNGYTPQFYMRTTDITGKVCLPEGVEMVMPGDNVKVTVSLINPVAMGEGQRFAIREGGRTVGAGVVSKVIG

Sequences:

>Translated_396_residues
MAQDKFKRTKLHVNVGTIGHVDHGKTTLTAALTKVGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYETEVRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDAELLELVEMEVRELLSK
YDFPGDDTPIVRGSALKALEGDQSEIGVPAIIRLAEALDTHIPNPERAIDRPFLMPVEDVFSISGRGTVVTGRIECGVIK
VGDEVEIVGIRPTSKTIVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDEVERGQVLAKPGCIKAHKEFEAEVYVLSKEEGG
RHTPFFNGYTPQFYMRTTDITGKVCLPEGVEMVMPGDNVKVTVSLINPVAMGEGQRFAIREGGRTVGAGVVSKVIG
>Mature_395_residues
AQDKFKRTKLHVNVGTIGHVDHGKTTLTAALTKVGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYETEVRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDAELLELVEMEVRELLSKY
DFPGDDTPIVRGSALKALEGDQSEIGVPAIIRLAEALDTHIPNPERAIDRPFLMPVEDVFSISGRGTVVTGRIECGVIKV
GDEVEIVGIRPTSKTIVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDEVERGQVLAKPGCIKAHKEFEAEVYVLSKEEGGR
HTPFFNGYTPQFYMRTTDITGKVCLPEGVEMVMPGDNVKVTVSLINPVAMGEGQRFAIREGGRTVGAGVVSKVIG

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.2025316455696, Blast_Score=480, Evalue=1e-135,
Organism=Homo sapiens, GI4503475, Length=454, Percent_Identity=29.5154185022026, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=29.2410714285714, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI223555963, Length=434, Percent_Identity=29.9539170506912, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5729864, Length=434, Percent_Identity=29.9539170506912, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI194018522, Length=439, Percent_Identity=28.4738041002278, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI194097354, Length=439, Percent_Identity=28.7015945330296, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI194018520, Length=439, Percent_Identity=28.7015945330296, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI46094014, Length=339, Percent_Identity=30.9734513274336, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI54607086, Length=318, Percent_Identity=30.188679245283, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4503507, Length=360, Percent_Identity=27.5, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=77.020202020202, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=76.9620253164557, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI2367247, Length=381, Percent_Identity=29.9212598425197, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI48994988, Length=237, Percent_Identity=32.9113924050633, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789108, Length=311, Percent_Identity=29.903536977492, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=396, Percent_Identity=53.2828282828283, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.4780361757106, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=455, Percent_Identity=32.3076923076923, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=455, Percent_Identity=32.3076923076923, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=436, Percent_Identity=30.9633027522936, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32566629, Length=351, Percent_Identity=30.4843304843305, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=27.2727272727273, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI115532065, Length=440, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17506081, Length=347, Percent_Identity=27.3775216138329, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=166, Percent_Identity=34.9397590361446, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=268, Percent_Identity=25.7462686567164, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=26.5822784810127, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=65.3164556962025, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=446, Percent_Identity=32.0627802690583, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=446, Percent_Identity=32.0627802690583, Blast_Score=166, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=31.2865497076023, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322937, Length=367, Percent_Identity=26.158038147139, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=269, Percent_Identity=28.6245353159851, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=28.1512605042017, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363316, Length=390, Percent_Identity=58.4615384615385, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17864358, Length=390, Percent_Identity=58.4615384615385, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI19921738, Length=382, Percent_Identity=50.7853403141361, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=30, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=30, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=30, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=30, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=30.5122494432071, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=30.5122494432071, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=31.1475409836066, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI17137380, Length=342, Percent_Identity=31.8713450292398, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI45550900, Length=340, Percent_Identity=29.7058823529412, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24647046, Length=361, Percent_Identity=27.7008310249307, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24647048, Length=361, Percent_Identity=27.7008310249307, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42907; Mature: 42776

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQDKFKRTKLHVNVGTIGHVDHGKTTLTAALTKVGAERFGGEFKAYDAIDAAPEEKARG
CCCCCCEEEEEEEEECEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHCC
ITISTAHVEYETEVRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIVVFLNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIVRGSALKALE
EEEHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHCC
GDQSEIGVPAIIRLAEALDTHIPNPERAIDRPFLMPVEDVFSISGRGTVVTGRIECGVIK
CCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEEEEEEEEE
VGDEVEIVGIRPTSKTIVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDEVERGQVLAKPGC
ECCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEEECCCC
IKAHKEFEAEVYVLSKEEGGRHTPFFNGYTPQFYMRTTDITGKVCLPEGVEMVMPGDNVK
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCCE
VTVSLINPVAMGEGQRFAIREGGRTVGAGVVSKVIG
EEEEEECEEECCCCCEEEEECCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AQDKFKRTKLHVNVGTIGHVDHGKTTLTAALTKVGAERFGGEFKAYDAIDAAPEEKARG
CCCCCEEEEEEEEECEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHCC
ITISTAHVEYETEVRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIVVFLNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIVRGSALKALE
EEEHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHCC
GDQSEIGVPAIIRLAEALDTHIPNPERAIDRPFLMPVEDVFSISGRGTVVTGRIECGVIK
CCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEEEEEEEEE
VGDEVEIVGIRPTSKTIVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDEVERGQVLAKPGC
ECCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEEECCCC
IKAHKEFEAEVYVLSKEEGGRHTPFFNGYTPQFYMRTTDITGKVCLPEGVEMVMPGDNVK
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCCE
VTVSLINPVAMGEGQRFAIREGGRTVGAGVVSKVIG
EEEEEECEEECCCCCEEEEECCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]