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The map label for this gene is tapB [H]

Identifier: 182682610

GI number: 182682610

Start: 2360414

End: 2360635

Strand: Reverse

Name: tapB [H]

Synonym: XfasM23_2098

Alternate gene names: 182682610

Gene position: 2360635-2360414 (Counterclockwise)

Preceding gene: 182682612

Following gene: 182682609

Centisome position: 93.1

GC content: 45.5

Gene sequence:

>222_bases
ATGTCTGATACGTCTGATGATCGTCAGATAGGTACGGTGAAGTGGTTCAATGATAATAAAGGCTTCGGCTTCATCACCTC
GAATAATGGGCCCGACCTGTTTGTCCATTACCGCGCAATTCAAGGTAATGGATTTAAGTCCCTTCAGGAAGGTCAGAAAG
TTTCTTTCGTTGCTGTGCAAGGCCAGAAAGGTATGCAGGCTGACCAAGTTCAGGTTCTGTAA

Upstream 100 bases:

>100_bases
ACCTGATCAGCTTCATCGTTCCGTTTGACCAACGCCTGCAACTCAGTTTTGGCCCCGATCTCCGGTGGCCGTTATCCATT
CAATTGTTCAGGAGTTTGTA

Downstream 100 bases:

>100_bases
TCTAGGGTCTCATTAGAGATTGTTCTCGTAACGCCGGAAGTACCTGCTTCCGGCTTTTTTCCGTGTTTTCCATAGATAAG
AATTTCTTATGGCTCATATC

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: E8.0 [H]

Number of amino acids: Translated: 73; Mature: 72

Protein sequence:

>73_residues
MSDTSDDRQIGTVKWFNDNKGFGFITSNNGPDLFVHYRAIQGNGFKSLQEGQKVSFVAVQGQKGMQADQVQVL

Sequences:

>Translated_73_residues
MSDTSDDRQIGTVKWFNDNKGFGFITSNNGPDLFVHYRAIQGNGFKSLQEGQKVSFVAVQGQKGMQADQVQVL
>Mature_72_residues
SDTSDDRQIGTVKWFNDNKGFGFITSNNGPDLFVHYRAIQGNGFKSLQEGQKVSFVAVQGQKGMQADQVQVL

Specific function: Affects cell viability at low temperatures [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=56.7164179104478, Blast_Score=75, Evalue=8e-16,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=57.8125, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=59.375, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI2367114, Length=68, Percent_Identity=52.9411764705882, Blast_Score=72, Evalue=9e-15,
Organism=Escherichia coli, GI1787834, Length=68, Percent_Identity=51.4705882352941, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=47.7611940298507, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1787107, Length=61, Percent_Identity=50.8196721311475, Blast_Score=63, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 8082; Mature: 7951

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTSDDRQIGTVKWFNDNKGFGFITSNNGPDLFVHYRAIQGNGFKSLQEGQKVSFVAVQ
CCCCCCCCEEEEEEEEECCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEE
GQKGMQADQVQVL
CCCCCCCCEEEEC
>Mature Secondary Structure 
SDTSDDRQIGTVKWFNDNKGFGFITSNNGPDLFVHYRAIQGNGFKSLQEGQKVSFVAVQ
CCCCCCCEEEEEEEEECCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEEE
GQKGMQADQVQVL
CCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9393697 [H]