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The map label for this gene is gacA [H]

Identifier: 182682601

GI number: 182682601

Start: 2350410

End: 2351072

Strand: Reverse

Name: gacA [H]

Synonym: XfasM23_2089

Alternate gene names: 182682601

Gene position: 2351072-2350410 (Counterclockwise)

Preceding gene: 182682602

Following gene: 182682599

Centisome position: 92.72

GC content: 46.3

Gene sequence:

>663_bases
ATGACTATTAAGATTTTTCTGATTGATGATCATACTCTCGTGCGTGTTGGCATGAAGATGATCTTATCCAATGAATTAGA
TCTCGAAGTGATAGGGGAAGCGGAGACAGGGGAGGCGGCTTTACCACAGATCCGTGAGCTACGCCCGAATGTCGTATTGT
GTGACATGCATCTCCCTGGGGTGAGTGGGCTGGAGATTACAGAAAAATTGGTGAAAGGGAATTATGGTAGCCGTGTAATT
ATTGTTTCGGTGTTGGAAGACGGCCCATTGCCGAAGCGACTGTTAGAGGCTGGAGCTTCTGGTTATGTTGGTAAGGGCGG
TGATGCTAATGAGTTGCTGCGTGCTATCCGTGAAGTGGCTTTAGGGAAGCGCTATCTTGGTAACAGCATTGCGCAGAATT
TGGTGTTGTCAAGCCTTGAAGGAGGATGTTCACCTTTTGATGTTTTGTCACCGCGTGAGTTGGAAATTGCCTTGTTGTTA
ATCCAAGGTCTGAGTCAGGGGGCTATTGCTAAGCGGTTGTGTCTCAGTCCTAAGACAATCAACACTCATAAAGTGCGTTT
GTTCGCTAAAGTTGATGTTCGAGATACCATTGCTCTGGCTCGGTTGGCTATTCAGTACGGGGTGAGTACTCCGGAGAAAT
ATAGTTTAGATAAAACGATCTGA

Upstream 100 bases:

>100_bases
CGATGCATTAATATGAGTATTTACCGCAATGTGTTTTGTTTCAGAAATTCGTTGGATTGACTCGCATCTCTAGAAAACTA
TCTGATTCAGGGGGACAAGG

Downstream 100 bases:

>100_bases
TTCGAAATCCAGATTTTCTTCCTCAAAATGATGCTTTCAGGTAGAGAGTGAACAGGTACTTAGAAGGCACTCAATAGGAT
GATATAAGGTGCTGCGTCAC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTIKIFLIDDHTLVRVGMKMILSNELDLEVIGEAETGEAALPQIRELRPNVVLCDMHLPGVSGLEITEKLVKGNYGSRVI
IVSVLEDGPLPKRLLEAGASGYVGKGGDANELLRAIREVALGKRYLGNSIAQNLVLSSLEGGCSPFDVLSPRELEIALLL
IQGLSQGAIAKRLCLSPKTINTHKVRLFAKVDVRDTIALARLAIQYGVSTPEKYSLDKTI

Sequences:

>Translated_220_residues
MTIKIFLIDDHTLVRVGMKMILSNELDLEVIGEAETGEAALPQIRELRPNVVLCDMHLPGVSGLEITEKLVKGNYGSRVI
IVSVLEDGPLPKRLLEAGASGYVGKGGDANELLRAIREVALGKRYLGNSIAQNLVLSSLEGGCSPFDVLSPRELEIALLL
IQGLSQGAIAKRLCLSPKTINTHKVRLFAKVDVRDTIALARLAIQYGVSTPEKYSLDKTI
>Mature_219_residues
TIKIFLIDDHTLVRVGMKMILSNELDLEVIGEAETGEAALPQIRELRPNVVLCDMHLPGVSGLEITEKLVKGNYGSRVII
VSVLEDGPLPKRLLEAGASGYVGKGGDANELLRAIREVALGKRYLGNSIAQNLVLSSLEGGCSPFDVLSPRELEIALLLI
QGLSQGAIAKRLCLSPKTINTHKVRLFAKVDVRDTIALARLAIQYGVSTPEKYSLDKTI

Specific function: Forms part of a two-component regulatory system gacA/gacA(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL) [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=215, Percent_Identity=40, Blast_Score=173, Evalue=6e-45,
Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=36.6834170854271, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1788521, Length=204, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1788712, Length=197, Percent_Identity=30.9644670050761, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1786747, Length=202, Percent_Identity=23.7623762376238, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=34.0659340659341, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788546, Length=201, Percent_Identity=29.3532338308458, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23824; Mature: 23693

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKIFLIDDHTLVRVGMKMILSNELDLEVIGEAETGEAALPQIRELRPNVVLCDMHLPG
CEEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCC
VSGLEITEKLVKGNYGSRVIIVSVLEDGPLPKRLLEAGASGYVGKGGDANELLRAIREVA
CCCHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LGKRYLGNSIAQNLVLSSLEGGCSPFDVLSPRELEIALLLIQGLSQGAIAKRLCLSPKTI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCC
NTHKVRLFAKVDVRDTIALARLAIQYGVSTPEKYSLDKTI
CCEEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIKIFLIDDHTLVRVGMKMILSNELDLEVIGEAETGEAALPQIRELRPNVVLCDMHLPG
EEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCC
VSGLEITEKLVKGNYGSRVIIVSVLEDGPLPKRLLEAGASGYVGKGGDANELLRAIREVA
CCCHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LGKRYLGNSIAQNLVLSSLEGGCSPFDVLSPRELEIALLLIQGLSQGAIAKRLCLSPKTI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCC
NTHKVRLFAKVDVRDTIALARLAIQYGVSTPEKYSLDKTI
CCEEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002569 [H]