Click here to switch to the map view.

The map label for this gene is murB

Identifier: 182682571

GI number: 182682571

Start: 2311049

End: 2312104

Strand: Direct

Name: murB

Synonym: XfasM23_2059

Alternate gene names: 182682571

Gene position: 2311049-2312104 (Clockwise)

Preceding gene: 182682570

Following gene: 182682572

Centisome position: 91.14

GC content: 55.21

Gene sequence:

>1056_bases
ATGAGCAGGCAAATCAATACTCCTGACTGGATCCTCCATGCCAACGCGCCTCTGCGTGAGCTGAACACTTTTCATATCCA
GGCACAAGCGCGCTGGCTACTAGAGATCATTCATCCGACCGCACTACCACAAGCATTGACGCACCCTCACATCGTCGGCT
TGCCAATCTTGGTGCTTGGCAGTGGCAGTAATGTTTTGTTCGCCGCCGATCCTGAGGAGTGTGTGCTGCGCTTTGTTAAC
CGCGAAGTGACGATCCTTGAACATCGGATCGATCACACGCTAGTACGTGCAGGTGCAGGGATGGCTTGGCATGACTTGGT
GCTATGGTCACTGCAACAGGGACTGTCTGGACTGGAAAATCTGGCACTAATTCCAGGCACAGTCGGAGCCTGCTCAATTC
AGAACATCGGTGCGTATGGGGTGCAAGTCGAAGAATTCGTGCACATTGTCGAAGCCTATGATCAGACCGAAGGCAAGTTC
GTAAGGCTGACCGCATCCGAATGTGAATTCGCTTACCGCAACAGTCGATTCAAGCGAGAACCAAACCGCTACCTGATCAC
AGCGGTAGAATTCCGTCTGCCGCTGCTGCATGAACTCAACCTCAACTACGCAGGGATCAGCGAAGAATTAGAGGCGCTAC
AGATCACATTGCCAGAGCCATGCGATGTCGCCCAGGCAGTTATCAATCTGCGTCGCCGCAAACTGCCGGATCCAGAGGTG
CTCAGCAACGCCGGCAGCTTTTTCAAGAACCCACATCTGCCACGCGAACAAGCCGAACAGTTACGCCAACACCACCCCAC
ACTCCCCATTTACCCGGGCGAAACACCAGAATCAAACAAGCTATCAGCGGCTTGGCTGATTGAGCAGTGTGGCTGGAAAG
GCATCCGCGAAGGCGACGCTGGAGTTGCGCCACAGCACTCACTTGTACTGGTTAACTACGGCGAAGCAACCGGCGCCGAG
CTGCTGGCGCTGGCACGACGCATCGCTGCTTCGGTGCAGGAGCGCTTCGGTGTGGCAATCGAGCCAGAAACACGCCTGAT
TGGGGCCCAATGGTGA

Upstream 100 bases:

>100_bases
ACACCGGATTGATATTCAAAGGCCCACAGTTGGTTGGAGAATGCGTGGAAGCCATTCGCCGCCGCCTCGAAGCCTCCAGT
AGCGGAAGGGAAAACACTCA

Downstream 100 bases:

>100_bases
CTCAGAGACAGCTGCCGCTACGTGCGGCAATGCTGATGCTATTCAGTACACTGTCGTTTGGCATGATGGCAATCACTATC
CGTTACGCTTCCACTCAAAT

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSRQINTPDWILHANAPLRELNTFHIQAQARWLLEIIHPTALPQALTHPHIVGLPILVLGSGSNVLFAADPEECVLRFVN
REVTILEHRIDHTLVRAGAGMAWHDLVLWSLQQGLSGLENLALIPGTVGACSIQNIGAYGVQVEEFVHIVEAYDQTEGKF
VRLTASECEFAYRNSRFKREPNRYLITAVEFRLPLLHELNLNYAGISEELEALQITLPEPCDVAQAVINLRRRKLPDPEV
LSNAGSFFKNPHLPREQAEQLRQHHPTLPIYPGETPESNKLSAAWLIEQCGWKGIREGDAGVAPQHSLVLVNYGEATGAE
LLALARRIAASVQERFGVAIEPETRLIGAQW

Sequences:

>Translated_351_residues
MSRQINTPDWILHANAPLRELNTFHIQAQARWLLEIIHPTALPQALTHPHIVGLPILVLGSGSNVLFAADPEECVLRFVN
REVTILEHRIDHTLVRAGAGMAWHDLVLWSLQQGLSGLENLALIPGTVGACSIQNIGAYGVQVEEFVHIVEAYDQTEGKF
VRLTASECEFAYRNSRFKREPNRYLITAVEFRLPLLHELNLNYAGISEELEALQITLPEPCDVAQAVINLRRRKLPDPEV
LSNAGSFFKNPHLPREQAEQLRQHHPTLPIYPGETPESNKLSAAWLIEQCGWKGIREGDAGVAPQHSLVLVNYGEATGAE
LLALARRIAASVQERFGVAIEPETRLIGAQW
>Mature_350_residues
SRQINTPDWILHANAPLRELNTFHIQAQARWLLEIIHPTALPQALTHPHIVGLPILVLGSGSNVLFAADPEECVLRFVNR
EVTILEHRIDHTLVRAGAGMAWHDLVLWSLQQGLSGLENLALIPGTVGACSIQNIGAYGVQVEEFVHIVEAYDQTEGKFV
RLTASECEFAYRNSRFKREPNRYLITAVEFRLPLLHELNLNYAGISEELEALQITLPEPCDVAQAVINLRRRKLPDPEVL
SNAGSFFKNPHLPREQAEQLRQHHPTLPIYPGETPESNKLSAAWLIEQCGWKGIREGDAGVAPQHSLVLVNYGEATGAEL
LALARRIAASVQERFGVAIEPETRLIGAQW

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=337, Percent_Identity=43.9169139465875, Blast_Score=239, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_XYLF2 (B2I9T6)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830731.1
- ProteinModelPortal:   B2I9T6
- SMR:   B2I9T6
- GeneID:   6203407
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_2059
- HOGENOM:   HBG686573
- OMA:   GAAPMQN
- ProtClustDB:   PRK00046
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 39132; Mature: 39001

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 173-173 ACT_SITE 246-246 ACT_SITE 343-343

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQINTPDWILHANAPLRELNTFHIQAQARWLLEIIHPTALPQALTHPHIVGLPILVLG
CCCCCCCCCEEEECCCCHHHCCEEEEEHHHHHHHHHHHHHHCHHHHCCCCEECCEEEEEE
SGSNVLFAADPEECVLRFVNREVTILEHRIDHTLVRAGAGMAWHDLVLWSLQQGLSGLEN
CCCCEEEECCHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
LALIPGTVGACSIQNIGAYGVQVEEFVHIVEAYDQTEGKFVRLTASECEFAYRNSRFKRE
CEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCC
PNRYLITAVEFRLPLLHELNLNYAGISEELEALQITLPEPCDVAQAVINLRRRKLPDPEV
CCCEEEEEEEECCCHHHHCCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHH
LSNAGSFFKNPHLPREQAEQLRQHHPTLPIYPGETPESNKLSAAWLIEQCGWKGIREGDA
HHHCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
GVAPQHSLVLVNYGEATGAELLALARRIAASVQERFGVAIEPETRLIGAQW
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCEECCCC
>Mature Secondary Structure 
SRQINTPDWILHANAPLRELNTFHIQAQARWLLEIIHPTALPQALTHPHIVGLPILVLG
CCCCCCCCEEEECCCCHHHCCEEEEEHHHHHHHHHHHHHHCHHHHCCCCEECCEEEEEE
SGSNVLFAADPEECVLRFVNREVTILEHRIDHTLVRAGAGMAWHDLVLWSLQQGLSGLEN
CCCCEEEECCHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
LALIPGTVGACSIQNIGAYGVQVEEFVHIVEAYDQTEGKFVRLTASECEFAYRNSRFKRE
CEECCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCCCCC
PNRYLITAVEFRLPLLHELNLNYAGISEELEALQITLPEPCDVAQAVINLRRRKLPDPEV
CCCEEEEEEEECCCHHHHCCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHH
LSNAGSFFKNPHLPREQAEQLRQHHPTLPIYPGETPESNKLSAAWLIEQCGWKGIREGDA
HHHCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
GVAPQHSLVLVNYGEATGAELLALARRIAASVQERFGVAIEPETRLIGAQW
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA