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The map label for this gene is ligA

Identifier: 182682559

GI number: 182682559

Start: 2295468

End: 2297963

Strand: Reverse

Name: ligA

Synonym: XfasM23_2047

Alternate gene names: 182682559

Gene position: 2297963-2295468 (Counterclockwise)

Preceding gene: 182682560

Following gene: 182682558

Centisome position: 90.62

GC content: 58.01

Gene sequence:

>2496_bases
ATGATCCCCCTGGATCCTGCCCAGCGTGCCGCTGAACTGCGTCGTCGTCTTCAAGAGGCCAACTACCACTACCACGTCCT
CGACCAACCACGTATTCCGGATGCTGACTACGACCGCATGTTGCGTGAACTGGACGCCCTCGAAGCGACCTATCCAGACC
TAGCGACGCCTGATTCACCGACTCAGCGTGTTGGACACACCATTGCCACCGCATTTTCCGAAGTGCGCCATACCGTCCCG
ATGCTTTCCCTGAACAATGCCTTCAGTGATCCCGAAGTCCTTGAATTCGTGCGCCGTATTACCGCGCGTCTGGGAGAAAC
AGCCCCTGGTTTTTCCGCAGAACCCAAACTGGATGGCTTGGCGATCAGCCTCCGCTATCAGAACGGCATCTTTATCCAAG
GAGCGACTCGCGGCGATGGCGTCACCGGCGAAGATGTCACCGCCAACCTGCGTACCCTGCCTACCATCCCGCAACGCTTG
CAAAGCGACACATGGCCAACCGTGTTGGAAGTGCGCGGCGAAGTGTATATGCCACGTCCTGACTTTGAAGCCTACAACAC
CCAGGCCCGGCTTCGCGGATGGAAAGTACTGGCCAATCCCCGCAATGGCGCCGCCGGCTCCTTGCGGCAACTGGACCCAC
ACATCACCGCACAGCGCCCCCTGAGCTTCTATGCCTACGGTATCGGCGAAGTGACCGATGACGTAGCGTTCCACCGCCAC
TCCGAAATCCTGGCCTCCCTACGTGCCTGGGGATTCCCCGTCTCCCCCCTCGTGGAGCTGGTGTATGGCAGTGAAGGACT
ACTGAATTACTACCGCCGGATGGAAACCATTCGCGATACCTTACCGTTTGATATTGATGGCATCGTTTATAAACTCGACG
ACCTGTCTGGCCAGCATGAAATGGGGTTTGTCGCCCGTGCCCCCCGTTGGGCCATTGCCCATAAATTTCCGGCCCAAGAA
CAAACCACCACTGTTGAAGCCATTGAAATCCAGATTGGCCGCACCGGTGCGGCCACCCCAGTCGCACGCCTGACCCCCGT
ACAAGTCGCTGGAGTCACCGTTACCAGCGTCACCTTGCATAACGCTGACCAGATCGCCCGCTTGGATGTACGCATCGGTG
ATACCGTCATCGTGCGCCGTGCTGGTGACGTGATTCCTGAAGTCGTCGCAGTCATCACCGACAGCCGCCCCCCTGGAGCC
ACCGCATGGAGCATGCCGATGGCGTGCCCCGTCTGTGGTTCCGAGATTGTTCGTGAAACAGGCGCCGCTGTATGGCGTTG
CTCCGGTGAACTCGCCTGTCCCGCCCAACGCAAAGAGGCCATCCGCCATTTCGTCTCCCGCCGCGCTATGGATGTCGAAG
GGCTGGGCGTCAAGTGCATTGAATTACTGGTGGATGCCGCCGTTGTCCACGGCGTCGCTGACCTGTATCACCTGAGCCTG
GATCAACTGCTGCGGCTCCGCCTCGTCACCAATGCCCAAACCCCCACCATGCTCCTGCGCGAAGCGCGCGACCATGTGAC
CGGCATGCGTTACCAACAATTAGAAGAGATACTGCGCACCGTGGGTGTGGACCTGTCTGGTGAAGGTGACGTGCCCAAGC
ATTGGCAGATTGACGTATTGCGTGCCCAATGGCCGGACTTTGATTGGAACCACAAAAAGATTGCCACCAAGTGGGCGCAG
AACCTGATTGCTGCCATTGACCGCTCACGGCAGACTACCTTGGAGCGTTTCCTCTTTGCCCTTGGCATGACCCATGTCGG
CGAAACCACCGCCAAAGCACTGGCGCACTCCTTCGGGGACTTAGCAATCATTCGGCAACTCCCTTGGCCCTTATTCAAAT
GCGTCCCGGATATTGGAGGCGAAGTGGCACGTGCCATCGGCCACTTTATGGATCAGCCCGCCAACCAACAGGCCATTGAT
GACCTAGTGGAGCGTGGCGTGCGTATCACCGATGCCCATCCCCCCACATCTACATTGCGCGATCAACTCACCCTGGCCAG
CCTCTTGGAACACTTGGAGATTCCAAAAATCACCCCACTGCGTGCTGTCCAGTTAGCCACCCTCGCTCCCACATTGCCCT
TATTGGCTGAAGCCGACCTTGATGCACTCCTTCAGGCGGGCGTGCCCCAGCCTGCTGCACAATCATTGACCGAATGGTTC
CAATCTCCTGATAACATCTCCTTAGCCCGCCGCCTCCAGCACTGCTGTGACGTACTGTTGGCACAACTGCTATCTCCCGA
TCGTGCCCACACTGCCCCACTCAACGGCCAGAGCGTCGTACTGACAGGCAAATTAGCGTCACTGACCCGTGAAGCCGCCG
CCACTCGACTAGAAAGCTTGGGAGCCAAAATTGTCGGCAGTGTGTCCAAAAAAACCAGCTTCCTTGTTGCTGGAGAAGAT
CCAGGCTCCAAACTCGACAAAGCCCACGCCTTGCATGTCGATATTTGGGATGAAGCCCGTCTACTCGCCTTTCTGGAACA
ATACAGTGCCCAATAG

Upstream 100 bases:

>100_bases
GTTTGTAACCATGCCCAACGCACCGAACAACCCTGCAACATCTCACAGCGCCTCACTTATACAATCATTTCCTGTTTTAT
AAATAACTGATCCCGCCCTC

Downstream 100 bases:

>100_bases
AACAACAGACATGGACACGGCTACCTTGCAATGGCGTCTTGCTACTCCTGTCGATGAACCCCTACTGATCCCCATGATCC
GGCGCTTCTATGCCGAAGAC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 831; Mature: 831

Protein sequence:

>831_residues
MIPLDPAQRAAELRRRLQEANYHYHVLDQPRIPDADYDRMLRELDALEATYPDLATPDSPTQRVGHTIATAFSEVRHTVP
MLSLNNAFSDPEVLEFVRRITARLGETAPGFSAEPKLDGLAISLRYQNGIFIQGATRGDGVTGEDVTANLRTLPTIPQRL
QSDTWPTVLEVRGEVYMPRPDFEAYNTQARLRGWKVLANPRNGAAGSLRQLDPHITAQRPLSFYAYGIGEVTDDVAFHRH
SEILASLRAWGFPVSPLVELVYGSEGLLNYYRRMETIRDTLPFDIDGIVYKLDDLSGQHEMGFVARAPRWAIAHKFPAQE
QTTTVEAIEIQIGRTGAATPVARLTPVQVAGVTVTSVTLHNADQIARLDVRIGDTVIVRRAGDVIPEVVAVITDSRPPGA
TAWSMPMACPVCGSEIVRETGAAVWRCSGELACPAQRKEAIRHFVSRRAMDVEGLGVKCIELLVDAAVVHGVADLYHLSL
DQLLRLRLVTNAQTPTMLLREARDHVTGMRYQQLEEILRTVGVDLSGEGDVPKHWQIDVLRAQWPDFDWNHKKIATKWAQ
NLIAAIDRSRQTTLERFLFALGMTHVGETTAKALAHSFGDLAIIRQLPWPLFKCVPDIGGEVARAIGHFMDQPANQQAID
DLVERGVRITDAHPPTSTLRDQLTLASLLEHLEIPKITPLRAVQLATLAPTLPLLAEADLDALLQAGVPQPAAQSLTEWF
QSPDNISLARRLQHCCDVLLAQLLSPDRAHTAPLNGQSVVLTGKLASLTREAAATRLESLGAKIVGSVSKKTSFLVAGED
PGSKLDKAHALHVDIWDEARLLAFLEQYSAQ

Sequences:

>Translated_831_residues
MIPLDPAQRAAELRRRLQEANYHYHVLDQPRIPDADYDRMLRELDALEATYPDLATPDSPTQRVGHTIATAFSEVRHTVP
MLSLNNAFSDPEVLEFVRRITARLGETAPGFSAEPKLDGLAISLRYQNGIFIQGATRGDGVTGEDVTANLRTLPTIPQRL
QSDTWPTVLEVRGEVYMPRPDFEAYNTQARLRGWKVLANPRNGAAGSLRQLDPHITAQRPLSFYAYGIGEVTDDVAFHRH
SEILASLRAWGFPVSPLVELVYGSEGLLNYYRRMETIRDTLPFDIDGIVYKLDDLSGQHEMGFVARAPRWAIAHKFPAQE
QTTTVEAIEIQIGRTGAATPVARLTPVQVAGVTVTSVTLHNADQIARLDVRIGDTVIVRRAGDVIPEVVAVITDSRPPGA
TAWSMPMACPVCGSEIVRETGAAVWRCSGELACPAQRKEAIRHFVSRRAMDVEGLGVKCIELLVDAAVVHGVADLYHLSL
DQLLRLRLVTNAQTPTMLLREARDHVTGMRYQQLEEILRTVGVDLSGEGDVPKHWQIDVLRAQWPDFDWNHKKIATKWAQ
NLIAAIDRSRQTTLERFLFALGMTHVGETTAKALAHSFGDLAIIRQLPWPLFKCVPDIGGEVARAIGHFMDQPANQQAID
DLVERGVRITDAHPPTSTLRDQLTLASLLEHLEIPKITPLRAVQLATLAPTLPLLAEADLDALLQAGVPQPAAQSLTEWF
QSPDNISLARRLQHCCDVLLAQLLSPDRAHTAPLNGQSVVLTGKLASLTREAAATRLESLGAKIVGSVSKKTSFLVAGED
PGSKLDKAHALHVDIWDEARLLAFLEQYSAQ
>Mature_831_residues
MIPLDPAQRAAELRRRLQEANYHYHVLDQPRIPDADYDRMLRELDALEATYPDLATPDSPTQRVGHTIATAFSEVRHTVP
MLSLNNAFSDPEVLEFVRRITARLGETAPGFSAEPKLDGLAISLRYQNGIFIQGATRGDGVTGEDVTANLRTLPTIPQRL
QSDTWPTVLEVRGEVYMPRPDFEAYNTQARLRGWKVLANPRNGAAGSLRQLDPHITAQRPLSFYAYGIGEVTDDVAFHRH
SEILASLRAWGFPVSPLVELVYGSEGLLNYYRRMETIRDTLPFDIDGIVYKLDDLSGQHEMGFVARAPRWAIAHKFPAQE
QTTTVEAIEIQIGRTGAATPVARLTPVQVAGVTVTSVTLHNADQIARLDVRIGDTVIVRRAGDVIPEVVAVITDSRPPGA
TAWSMPMACPVCGSEIVRETGAAVWRCSGELACPAQRKEAIRHFVSRRAMDVEGLGVKCIELLVDAAVVHGVADLYHLSL
DQLLRLRLVTNAQTPTMLLREARDHVTGMRYQQLEEILRTVGVDLSGEGDVPKHWQIDVLRAQWPDFDWNHKKIATKWAQ
NLIAAIDRSRQTTLERFLFALGMTHVGETTAKALAHSFGDLAIIRQLPWPLFKCVPDIGGEVARAIGHFMDQPANQQAID
DLVERGVRITDAHPPTSTLRDQLTLASLLEHLEIPKITPLRAVQLATLAPTLPLLAEADLDALLQAGVPQPAAQSLTEWF
QSPDNISLARRLQHCCDVLLAQLLSPDRAHTAPLNGQSVVLTGKLASLTREAAATRLESLGAKIVGSVSKKTSFLVAGED
PGSKLDKAHALHVDIWDEARLLAFLEQYSAQ

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=641, Percent_Identity=45.8658346333853, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI87082305, Length=287, Percent_Identity=25.7839721254355, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_XYLF2 (B2I9S4)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830719.1
- ProteinModelPortal:   B2I9S4
- GeneID:   6203393
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_2047
- HOGENOM:   HBG620317
- OMA:   FHELKAL
- ProtClustDB:   PRK07956
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 91617; Mature: 91617

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 116-116 BINDING 114-114 BINDING 137-137 BINDING 174-174 BINDING 291-291 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPLDPAQRAAELRRRLQEANYHYHVLDQPRIPDADYDRMLRELDALEATYPDLATPDSP
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCH
TQRVGHTIATAFSEVRHTVPMLSLNNAFSDPEVLEFVRRITARLGETAPGFSAEPKLDGL
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
AISLRYQNGIFIQGATRGDGVTGEDVTANLRTLPTIPQRLQSDTWPTVLEVRGEVYMPRP
EEEEEECCCEEEEECCCCCCCCCCCCCCCCHHCCCHHHHHCCCCCCHHEEECCCEECCCC
DFEAYNTQARLRGWKVLANPRNGAAGSLRQLDPHITAQRPLSFYAYGIGEVTDDVAFHRH
CCHHHCCHHHHCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
SEILASLRAWGFPVSPLVELVYGSEGLLNYYRRMETIRDTLPFDIDGIVYKLDDLSGQHE
HHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
MGFVARAPRWAIAHKFPAQEQTTTVEAIEIQIGRTGAATPVARLTPVQVAGVTVTSVTLH
CCHHHCCCCHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCHHHCCCEEEECEEEEEEEEC
NADQIARLDVRIGDTVIVRRAGDVIPEVVAVITDSRPPGATAWSMPMACPVCGSEIVRET
CCCHHEEEEEEECCEEEEECHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHH
GAAVWRCSGELACPAQRKEAIRHFVSRRAMDVEGLGVKCIELLVDAAVVHGVADLYHLSL
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCH
DQLLRLRLVTNAQTPTMLLREARDHVTGMRYQQLEEILRTVGVDLSGEGDVPKHWQIDVL
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
RAQWPDFDWNHKKIATKWAQNLIAAIDRSRQTTLERFLFALGMTHVGETTAKALAHSFGD
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LAIIRQLPWPLFKCVPDIGGEVARAIGHFMDQPANQQAIDDLVERGVRITDAHPPTSTLR
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCHHHHH
DQLTLASLLEHLEIPKITPLRAVQLATLAPTLPLLAEADLDALLQAGVPQPAAQSLTEWF
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCHHHHHHCCCCCHHHHHHHHHH
QSPDNISLARRLQHCCDVLLAQLLSPDRAHTAPLNGQSVVLTGKLASLTREAAATRLESL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHH
GAKIVGSVSKKTSFLVAGEDPGSKLDKAHALHVDIWDEARLLAFLEQYSAQ
HHHHHHHHCCCCEEEEECCCCCCHHCCCCEEEEEECHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPLDPAQRAAELRRRLQEANYHYHVLDQPRIPDADYDRMLRELDALEATYPDLATPDSP
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCH
TQRVGHTIATAFSEVRHTVPMLSLNNAFSDPEVLEFVRRITARLGETAPGFSAEPKLDGL
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
AISLRYQNGIFIQGATRGDGVTGEDVTANLRTLPTIPQRLQSDTWPTVLEVRGEVYMPRP
EEEEEECCCEEEEECCCCCCCCCCCCCCCCHHCCCHHHHHCCCCCCHHEEECCCEECCCC
DFEAYNTQARLRGWKVLANPRNGAAGSLRQLDPHITAQRPLSFYAYGIGEVTDDVAFHRH
CCHHHCCHHHHCCEEEEECCCCCCCCCHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHH
SEILASLRAWGFPVSPLVELVYGSEGLLNYYRRMETIRDTLPFDIDGIVYKLDDLSGQHE
HHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
MGFVARAPRWAIAHKFPAQEQTTTVEAIEIQIGRTGAATPVARLTPVQVAGVTVTSVTLH
CCHHHCCCCHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCHHHCCCEEEECEEEEEEEEC
NADQIARLDVRIGDTVIVRRAGDVIPEVVAVITDSRPPGATAWSMPMACPVCGSEIVRET
CCCHHEEEEEEECCEEEEECHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHH
GAAVWRCSGELACPAQRKEAIRHFVSRRAMDVEGLGVKCIELLVDAAVVHGVADLYHLSL
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCH
DQLLRLRLVTNAQTPTMLLREARDHVTGMRYQQLEEILRTVGVDLSGEGDVPKHWQIDVL
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
RAQWPDFDWNHKKIATKWAQNLIAAIDRSRQTTLERFLFALGMTHVGETTAKALAHSFGD
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LAIIRQLPWPLFKCVPDIGGEVARAIGHFMDQPANQQAIDDLVERGVRITDAHPPTSTLR
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCHHHHH
DQLTLASLLEHLEIPKITPLRAVQLATLAPTLPLLAEADLDALLQAGVPQPAAQSLTEWF
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCHHHHHHCCCCCHHHHHHHHHH
QSPDNISLARRLQHCCDVLLAQLLSPDRAHTAPLNGQSVVLTGKLASLTREAAATRLESL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHH
GAKIVGSVSKKTSFLVAGEDPGSKLDKAHALHVDIWDEARLLAFLEQYSAQ
HHHHHHHHCCCCEEEEECCCCCCHHCCCCEEEEEECHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA