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The map label for this gene is rimK

Identifier: 182682536

GI number: 182682536

Start: 2265585

End: 2266490

Strand: Direct

Name: rimK

Synonym: XfasM23_2024

Alternate gene names: 182682536

Gene position: 2265585-2266490 (Clockwise)

Preceding gene: 182682535

Following gene: 182682537

Centisome position: 89.35

GC content: 54.97

Gene sequence:

>906_bases
ATGAAGCTGGCCATTCTCTCCCGCAACAGCAAGCTTTATTCCACTCGACGTTTAGTCGAAGTCGCACGTATGCGTGGCCA
TACTGTTCGTATTCTTGACCCGTTACGCTGCTACATGCGGATCGTAGTTGGAGACTTCAGCATGCACTATAAAGGCAAGC
CCATTGATGGCTATCACGCGGTGATTCCTCGCATTGGTGTATCGGTCACGCATTACGCCGCAGCGGTGCTACGTCAATTC
GAACTGATGGGCACCTACAGCCCCAATCCCTCGGACGCAATTCTGCGCTCACGAGATAAGCTACGCGCACATCAACTGTT
AGCCGCCCAGGGGATCGACATGCCAATGACTGTGTTCGGCGACAATCCCGACGATACTCAAGACCTACTCTCAATGCTCG
GTCCGCCCCCCCATGTAGTCAAGCTGAATGAGGGCGCGCAGGGTAAGGGAGTGATCCTATCGGAAAAAAACAGTGCTTCA
CGCGGTTTGGTAGAGGCACTACGTGGGCTCTACGCCAATTTTCTGGTACAGGAATTTATAAGCGAAGCTGACAGTGCGGA
CCTGCGCTGCTTCGTCGTCGGCAACCAAGTCGTAGCAACGATGCGTCGCCAAGCTGCGGAGGGTGACTTTCGTTCCAACC
TGCACCTAGGTGGGAGTGCAACTGCCGCCACCGCAAGCGAGGAAGAACAAGAGGTGGCAGTACGTTCAGCACACGCACTT
GGACTGACCGTGGCCGGTGTAGATCTCATCCGTTCACGACGTGGACCGCTGGTCCTTGAGGTCAATCCCACCCCGGGGTT
GGAAGGCATCGAAGCGACCTCCGGAACCAACGTCGCCATAAAGATTGTGCGCCATGTGGAAGAAATGCTGGCTACTATCT
GTAGCAGCAATATTTGCAAGCCCTGA

Upstream 100 bases:

>100_bases
AAATTCTCCATACCGATTACCCGTTGCATCTTTCCATGCTCCACCGCTAGGTAGAGCGTTTTGCATTCATAACATCCAAG
ATACATTCGGTTGAATACTG

Downstream 100 bases:

>100_bases
TGGATTATTTAGGTCATAGCTACAACTTATCGATCACAGCACTTACCTCACTGATCATGGCATCAAAATTTATTTTTTGA
TTAAAGCACTTTTGCTTAGG

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKLAILSRNSKLYSTRRLVEVARMRGHTVRILDPLRCYMRIVVGDFSMHYKGKPIDGYHAVIPRIGVSVTHYAAAVLRQF
ELMGTYSPNPSDAILRSRDKLRAHQLLAAQGIDMPMTVFGDNPDDTQDLLSMLGPPPHVVKLNEGAQGKGVILSEKNSAS
RGLVEALRGLYANFLVQEFISEADSADLRCFVVGNQVVATMRRQAAEGDFRSNLHLGGSATAATASEEEQEVAVRSAHAL
GLTVAGVDLIRSRRGPLVLEVNPTPGLEGIEATSGTNVAIKIVRHVEEMLATICSSNICKP

Sequences:

>Translated_301_residues
MKLAILSRNSKLYSTRRLVEVARMRGHTVRILDPLRCYMRIVVGDFSMHYKGKPIDGYHAVIPRIGVSVTHYAAAVLRQF
ELMGTYSPNPSDAILRSRDKLRAHQLLAAQGIDMPMTVFGDNPDDTQDLLSMLGPPPHVVKLNEGAQGKGVILSEKNSAS
RGLVEALRGLYANFLVQEFISEADSADLRCFVVGNQVVATMRRQAAEGDFRSNLHLGGSATAATASEEEQEVAVRSAHAL
GLTVAGVDLIRSRRGPLVLEVNPTPGLEGIEATSGTNVAIKIVRHVEEMLATICSSNICKP
>Mature_301_residues
MKLAILSRNSKLYSTRRLVEVARMRGHTVRILDPLRCYMRIVVGDFSMHYKGKPIDGYHAVIPRIGVSVTHYAAAVLRQF
ELMGTYSPNPSDAILRSRDKLRAHQLLAAQGIDMPMTVFGDNPDDTQDLLSMLGPPPHVVKLNEGAQGKGVILSEKNSAS
RGLVEALRGLYANFLVQEFISEADSADLRCFVVGNQVVATMRRQAAEGDFRSNLHLGGSATAATASEEEQEVAVRSAHAL
GLTVAGVDLIRSRRGPLVLEVNPTPGLEGIEATSGTNVAIKIVRHVEEMLATICSSNICKP

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI134254462, Length=264, Percent_Identity=28.7878787878788, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI164663810, Length=219, Percent_Identity=27.8538812785388, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1787076, Length=292, Percent_Identity=54.7945205479452, Blast_Score=333, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMK_XYLF2 (B2I9Q1)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830696.1
- ProteinModelPortal:   B2I9Q1
- GeneID:   6203617
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_2024
- HOGENOM:   HBG679591
- OMA:   CREPRLY
- ProtClustDB:   CLSK451700
- GO:   GO:0006412
- HAMAP:   MF_01552
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 32681; Mature: 32681

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAILSRNSKLYSTRRLVEVARMRGHTVRILDPLRCYMRIVVGDFSMHYKGKPIDGYHA
CEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCEECCCCCCCCCHHH
VIPRIGVSVTHYAAAVLRQFELMGTYSPNPSDAILRSRDKLRAHQLLAAQGIDMPMTVFG
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
DNPDDTQDLLSMLGPPPHVVKLNEGAQGKGVILSEKNSASRGLVEALRGLYANFLVQEFI
CCCCCHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
SEADSADLRCFVVGNQVVATMRRQAAEGDFRSNLHLGGSATAATASEEEQEVAVRSAHAL
HCCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHH
GLTVAGVDLIRSRRGPLVLEVNPTPGLEGIEATSGTNVAIKIVRHVEEMLATICSSNICK
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
P
C
>Mature Secondary Structure
MKLAILSRNSKLYSTRRLVEVARMRGHTVRILDPLRCYMRIVVGDFSMHYKGKPIDGYHA
CEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCEECCCCCCCCCHHH
VIPRIGVSVTHYAAAVLRQFELMGTYSPNPSDAILRSRDKLRAHQLLAAQGIDMPMTVFG
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
DNPDDTQDLLSMLGPPPHVVKLNEGAQGKGVILSEKNSASRGLVEALRGLYANFLVQEFI
CCCCCHHHHHHHHCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
SEADSADLRCFVVGNQVVATMRRQAAEGDFRSNLHLGGSATAATASEEEQEVAVRSAHAL
HCCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHH
GLTVAGVDLIRSRRGPLVLEVNPTPGLEGIEATSGTNVAIKIVRHVEEMLATICSSNICK
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA