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The map label for this gene is cysB [H]

Identifier: 182682453

GI number: 182682453

Start: 2170528

End: 2171559

Strand: Reverse

Name: cysB [H]

Synonym: XfasM23_1940

Alternate gene names: 182682453

Gene position: 2171559-2170528 (Counterclockwise)

Preceding gene: 182682457

Following gene: 182682452

Centisome position: 85.64

GC content: 55.14

Gene sequence:

>1032_bases
ATGGAAGTGATCTACAGTCGTTTTTGGTTTTGCGATATTTCGCCTGTCATGACGTTGACTCAACTTCGCTACCTTGTGGC
TATTGCCGATGCCGATCTCAATATTACGCTTGCTGCTGCACGTATACATGCCACGCAGCCGGGCCTGTCCAAGCAATTGA
AAAAGCTAGAGGATGAACTCGGCTTTTTGTTGTTTGTGCGCCGGGGCCGTAGTTTGGAGTCAGTGACGCCTGCGGGGGAG
GAGGTGATTGAGCGGGCGCGGGTGATGTTGGTTGAAGTCAACAACATACGTACTTATGCCGCCAATCAGCGCCGTGAGTA
CCAAGGGCAATTGACGTTGAGTACCACCCACACTCAGGCGCGTTTTGTGCTGCCGCCTGCCGTGGCGTTGATCAAGCAGG
CTTATCCGCAGGTTACTGTGCATTTGCAGCAGGCTGCCGAGAGCGATGTGCTGGATGAGTTTGGTCAGGGAGGGGCCGAT
ATTGCAATCGTGAGTACTGCGGGCAGCGCGCCTTCATCAGGGCTTGCGGTCCCTTTGTATCGGTGGCGGCGTTTGGTGAT
TGTGCCGTGTGGTCATCCTTTGGATACAGTGGGGTGCACTCTGGATTTGGCAGCGCTGGCGGAGTATCCGTTGGTCAGTT
ATGACTCTTCAATACGGCCAATGTCTTCGCTGCAACGCGCTTTCGCCAGTTTAGGGTTGGAGTCGCGCATTGTGTTGACA
GCTTTGGATGCTGATTTGATCAAGACCTATGTGCGTGCCGGTCTGGGTGTGGGCATTCTTGCGGAGATGGCGGTTAATGC
GGTCGATACCGATTTGCGTGCTTGGCCGGTACCGGCGCCCATTCCTGAATGTATTGCTTGGGCGGTGCTGCCACGTGATC
GTGTCTTGCGTGACTACGTGTTGGAGTTGGTGCATGCGCTTGCGCCGCAGATCGACAAGCGTGATTTGCGGCGTGTGCTC
AATGGTCATCAGGAGCCTGACTGGCCGTCGCCTCCTAGCTGGCAATCGTTGACACAGACCATTACTCATTGA

Upstream 100 bases:

>100_bases
AGTGAGTTGAGGTCGGGGGTCATGACCGTAGTGCCTGGTTGATAATCTCAGGAAATGACTTCAGGCAGTTGGAAAATAGC
CGTTAGTTATTAAGTTCTTC

Downstream 100 bases:

>100_bases
GTGGGTTCTCAAGTGGCTGTGTGGGGGCTATGGATGTTCTTGGTGAGTCACGCAGGTGGTGGCAGGGTGTGTGATGTGTT
CACGGGTGCTTGCAGTCACT

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MEVIYSRFWFCDISPVMTLTQLRYLVAIADADLNITLAAARIHATQPGLSKQLKKLEDELGFLLFVRRGRSLESVTPAGE
EVIERARVMLVEVNNIRTYAANQRREYQGQLTLSTTHTQARFVLPPAVALIKQAYPQVTVHLQQAAESDVLDEFGQGGAD
IAIVSTAGSAPSSGLAVPLYRWRRLVIVPCGHPLDTVGCTLDLAALAEYPLVSYDSSIRPMSSLQRAFASLGLESRIVLT
ALDADLIKTYVRAGLGVGILAEMAVNAVDTDLRAWPVPAPIPECIAWAVLPRDRVLRDYVLELVHALAPQIDKRDLRRVL
NGHQEPDWPSPPSWQSLTQTITH

Sequences:

>Translated_343_residues
MEVIYSRFWFCDISPVMTLTQLRYLVAIADADLNITLAAARIHATQPGLSKQLKKLEDELGFLLFVRRGRSLESVTPAGE
EVIERARVMLVEVNNIRTYAANQRREYQGQLTLSTTHTQARFVLPPAVALIKQAYPQVTVHLQQAAESDVLDEFGQGGAD
IAIVSTAGSAPSSGLAVPLYRWRRLVIVPCGHPLDTVGCTLDLAALAEYPLVSYDSSIRPMSSLQRAFASLGLESRIVLT
ALDADLIKTYVRAGLGVGILAEMAVNAVDTDLRAWPVPAPIPECIAWAVLPRDRVLRDYVLELVHALAPQIDKRDLRRVL
NGHQEPDWPSPPSWQSLTQTITH
>Mature_343_residues
MEVIYSRFWFCDISPVMTLTQLRYLVAIADADLNITLAAARIHATQPGLSKQLKKLEDELGFLLFVRRGRSLESVTPAGE
EVIERARVMLVEVNNIRTYAANQRREYQGQLTLSTTHTQARFVLPPAVALIKQAYPQVTVHLQQAAESDVLDEFGQGGAD
IAIVSTAGSAPSSGLAVPLYRWRRLVIVPCGHPLDTVGCTLDLAALAEYPLVSYDSSIRPMSSLQRAFASLGLESRIVLT
ALDADLIKTYVRAGLGVGILAEMAVNAVDTDLRAWPVPAPIPECIAWAVLPRDRVLRDYVLELVHALAPQIDKRDLRRVL
NGHQEPDWPSPPSWQSLTQTITH

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=298, Percent_Identity=38.9261744966443, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1788296, Length=308, Percent_Identity=33.4415584415584, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1790399, Length=251, Percent_Identity=28.2868525896414, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787879, Length=303, Percent_Identity=27.0627062706271, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367136, Length=244, Percent_Identity=26.6393442622951, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787806, Length=177, Percent_Identity=29.3785310734463, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=26.1168384879725, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=225, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788297, Length=262, Percent_Identity=23.6641221374046, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=181, Percent_Identity=30.3867403314917, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=147, Percent_Identity=26.530612244898, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37832; Mature: 37832

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVIYSRFWFCDISPVMTLTQLRYLVAIADADLNITLAAARIHATQPGLSKQLKKLEDEL
CCCCEEEEEEECCHHHHHHHHHHHHHHEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHH
GFLLFVRRGRSLESVTPAGEEVIERARVMLVEVNNIRTYAANQRREYQGQLTLSTTHTQA
CEEEEECCCCCCCCCCCCHHHHHHHHHEEEEEECCCEEHHHCCCHHCCCEEEEEECCCCE
RFVLPPAVALIKQAYPQVTVHLQQAAESDVLDEFGQGGADIAIVSTAGSAPSSGLAVPLY
EEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEHH
RWRRLVIVPCGHPLDTVGCTLDLAALAEYPLVSYDSSIRPMSSLQRAFASLGLESRIVLT
HCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
ALDADLIKTYVRAGLGVGILAEMAVNAVDTDLRAWPVPAPIPECIAWAVLPRDRVLRDYV
EHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHH
LELVHALAPQIDKRDLRRVLNGHQEPDWPSPPSWQSLTQTITH
HHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MEVIYSRFWFCDISPVMTLTQLRYLVAIADADLNITLAAARIHATQPGLSKQLKKLEDEL
CCCCEEEEEEECCHHHHHHHHHHHHHHEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHH
GFLLFVRRGRSLESVTPAGEEVIERARVMLVEVNNIRTYAANQRREYQGQLTLSTTHTQA
CEEEEECCCCCCCCCCCCHHHHHHHHHEEEEEECCCEEHHHCCCHHCCCEEEEEECCCCE
RFVLPPAVALIKQAYPQVTVHLQQAAESDVLDEFGQGGADIAIVSTAGSAPSSGLAVPLY
EEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEHH
RWRRLVIVPCGHPLDTVGCTLDLAALAEYPLVSYDSSIRPMSSLQRAFASLGLESRIVLT
HCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
ALDADLIKTYVRAGLGVGILAEMAVNAVDTDLRAWPVPAPIPECIAWAVLPRDRVLRDYV
EHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHH
LELVHALAPQIDKRDLRRVLNGHQEPDWPSPPSWQSLTQTITH
HHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]