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The map label for this gene is mrcB [H]

Identifier: 182682413

GI number: 182682413

Start: 2112562

End: 2114940

Strand: Direct

Name: mrcB [H]

Synonym: XfasM23_1899

Alternate gene names: 182682413

Gene position: 2112562-2114940 (Clockwise)

Preceding gene: 182682403

Following gene: 182682414

Centisome position: 83.31

GC content: 57.04

Gene sequence:

>2379_bases
ATGCCGGGCCATTCCCCCTGGTGGGGCTGGCTGATCGCTTGTGGCTTGGCAGGCTTGGCGCTTGCCATGGGCTTTCTGAT
CCCTTACACGCTTTACCTGAATCAGCAGGTGACCGCTCGCTTTGGTGAGCTGCGCTGGCAGATCCCAACCCGGGTCTATG
CTCGCCCTTTGATGCTACAACCGGGGATGGCATTAGATGCGCGCACGTTGAAGATCGAACTGGATGCTGCATCCTATCGT
GAGGACAATCACGGCGAGTTACCAGGCACTTACCAACAACAAAATGGCCGATTCACTGTCTCCAGCCGTGGCTACGTTGA
TGTGGATGGTGCAATACCAGCAAGCCGTCTGATCATCACGCTGAACAACAACCAAGTGTCTGCACTGCGGAATGCAGATA
CACGTAAACCAATCAGGCGCGGGCGACTGGATCCAGCACGGATCGCCACACTGTACGGCCAGAAGCAGGAAGAACGCCGT
TTGGTCAGGTTGGAGGAAGTTCCCCAACTGCTCGTCACCGGCTTACAAGTGGTGGAAGACCGCGATTTCAAACATCACCA
CGGCATCGACATCACCAGCATTTTGCGCGCAGCCTGGGTGATGGTGCGTTCAGGCGGTGGCGTTCGCCAAGGTGCCAGTA
CCCTAACGCAACAGCTGGCGCGTAGCGGACTGCTTGGTATCGGCAAAGAACAGACGTTGCAGCGCAAATGCAACGAAATC
CTGTATGCACTGATCCTGGAGGCACGCTACTCCAAGCGCGTTATCCTGGAGTCCTATCTCAATCAAGTCTACCTGGGTCA
ACGTGGCAGTCAGGCCGTACATGGTGTCGCAACTGGCGCGGAGTTCTGGTATGGACGTGAGCTAGGCTCGCTCACTACTG
AGCAGGTCGCCCTGCTCATCGGACTGGTCAAAGGACCTTCGTACTACGATCCACGCCGTTTCCCTGATCGCGCATTGTCC
CGACGCAATTTCGTGCTTGGCAAACTGCATGAAAACAAACTGATCGACGATGCTGAATACATGCGCGCCCAAGCATCGCC
CCTAGGTGTCCCCAAAGAACCTGGCCTGGTTGCAGCCAACCGCTTCGTCGCGTACCTGGACCTGGTACGCCGCCAGCTTG
CTCACGATTACCCAGAGAATGTGTTGCGTGGCGCAGGCATGACCGTACTTACCGGCATGTCGCCCTCAGCACAAGCCTAT
GCTGAGAGAGGAGTAAAAACCACGCTCAGTGCACTGGAAAAGGTCAAGGGGCTGAAACGTCCGCCACTGCAAGCCGGACT
GGTACTGACGGACGTGCATAACGGCGATGTCCTGGCCGTGGTCGGTAGTCGCGATGCAACCCAATCGGGTTTCAATCGTG
CCATTGAAGCGCAACGCCAGGTGGGTTCGCTGCTGAAACCGTTCGTCTATCTACTAGCACTGGCCTCACCGGATCGTTGG
TCCTTGTCCAGTTGGGTAGAAGACACCCCGGTCAACATACCGCTGGCACATGGGCGCATTTGGTCGCCAAGCAACTCAGA
CAACATCAGCCACGGAACCGTGCGCTTGATTGATGCATTGGCACACTCTTACAACCAAGCAACAGTGCGCGTTGGCATGC
AGGTCGGCCCAGAACGTGTGGCTCAATTGATTAACGTTCTGGCCGGATTAAAAGCCGACGCCAACCCATCGCTGATCCTG
GGCGCAACCGACCAAAGCCCTTATGCAATGACGCAGCTCTATCAGTTCCTGGCTTCTGGGGGTGAAATCCAACCATTGCA
CGCAGTGCGTGGCGTACTCGATCCGCAAAACAAACTGCTCAAACGCTATGACAATACCCCGGCACCTGCACAGCCCGGTG
ATTCAGTCGCAGCCAACGTCATCAGTGTTGCGCTACAGGAAGTGGTCAACAGCGGTACCGCGCGGCAACTCATCATTGAT
GGCTTGGGTCGGCTGTCCCCTGCCGGCAAAACCGGAACCTCCAACGATGGACGTGACAGCTGGTACGCGGGCTATACCGG
GAACCACTTGGCTGTCATTTGGGTCGGCAACGACCAAAACGAGCAAACTGGCCTATACGGCGCCACCGGTGCCATGCGCG
TCTGGTCGAGCATCTTCTCGCAGTTACCAAGCACCAGATTACAAGTCAACGGCAAAGGACTGGATTGGCAATCAGTCGAG
CCTATGGGCACTGGCACCACAGACGCAAACTGCCCAGGCGCACGCCGCTTTCCGTTTGTAACCGGCTTCGCTCCGCCTTA
TACAGCATGCTCTGGTGCTGGCGGAGCACCCGAAGCCGGGCATAATATCGGCTGGCGTAACTGGTTCGGGCTGGATAAGC
AGAAGGGGGACCAAGCATCGCCCCCCATAACGCCTCCTCCCGATCAATCGACACAATGA

Upstream 100 bases:

>100_bases
AGACATGTAGGTGTTGCGCTAGACTCCATAAAGTCCTAATTCTCACATACCCGTGGCATACCACGCAGAAGATGATTTCA
ACGAAGCGTCAAATGGCAAC

Downstream 100 bases:

>100_bases
AAAGATTGCATGTTCCGTTCCGCGCATTCACCGTTACCGGCATGATGGCCTTGGCCCTTACTGCCTGTATCAGCCCGCCG
CAACCTGCAACACCAGCACC

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 792; Mature: 791

Protein sequence:

>792_residues
MPGHSPWWGWLIACGLAGLALAMGFLIPYTLYLNQQVTARFGELRWQIPTRVYARPLMLQPGMALDARTLKIELDAASYR
EDNHGELPGTYQQQNGRFTVSSRGYVDVDGAIPASRLIITLNNNQVSALRNADTRKPIRRGRLDPARIATLYGQKQEERR
LVRLEEVPQLLVTGLQVVEDRDFKHHHGIDITSILRAAWVMVRSGGGVRQGASTLTQQLARSGLLGIGKEQTLQRKCNEI
LYALILEARYSKRVILESYLNQVYLGQRGSQAVHGVATGAEFWYGRELGSLTTEQVALLIGLVKGPSYYDPRRFPDRALS
RRNFVLGKLHENKLIDDAEYMRAQASPLGVPKEPGLVAANRFVAYLDLVRRQLAHDYPENVLRGAGMTVLTGMSPSAQAY
AERGVKTTLSALEKVKGLKRPPLQAGLVLTDVHNGDVLAVVGSRDATQSGFNRAIEAQRQVGSLLKPFVYLLALASPDRW
SLSSWVEDTPVNIPLAHGRIWSPSNSDNISHGTVRLIDALAHSYNQATVRVGMQVGPERVAQLINVLAGLKADANPSLIL
GATDQSPYAMTQLYQFLASGGEIQPLHAVRGVLDPQNKLLKRYDNTPAPAQPGDSVAANVISVALQEVVNSGTARQLIID
GLGRLSPAGKTGTSNDGRDSWYAGYTGNHLAVIWVGNDQNEQTGLYGATGAMRVWSSIFSQLPSTRLQVNGKGLDWQSVE
PMGTGTTDANCPGARRFPFVTGFAPPYTACSGAGGAPEAGHNIGWRNWFGLDKQKGDQASPPITPPPDQSTQ

Sequences:

>Translated_792_residues
MPGHSPWWGWLIACGLAGLALAMGFLIPYTLYLNQQVTARFGELRWQIPTRVYARPLMLQPGMALDARTLKIELDAASYR
EDNHGELPGTYQQQNGRFTVSSRGYVDVDGAIPASRLIITLNNNQVSALRNADTRKPIRRGRLDPARIATLYGQKQEERR
LVRLEEVPQLLVTGLQVVEDRDFKHHHGIDITSILRAAWVMVRSGGGVRQGASTLTQQLARSGLLGIGKEQTLQRKCNEI
LYALILEARYSKRVILESYLNQVYLGQRGSQAVHGVATGAEFWYGRELGSLTTEQVALLIGLVKGPSYYDPRRFPDRALS
RRNFVLGKLHENKLIDDAEYMRAQASPLGVPKEPGLVAANRFVAYLDLVRRQLAHDYPENVLRGAGMTVLTGMSPSAQAY
AERGVKTTLSALEKVKGLKRPPLQAGLVLTDVHNGDVLAVVGSRDATQSGFNRAIEAQRQVGSLLKPFVYLLALASPDRW
SLSSWVEDTPVNIPLAHGRIWSPSNSDNISHGTVRLIDALAHSYNQATVRVGMQVGPERVAQLINVLAGLKADANPSLIL
GATDQSPYAMTQLYQFLASGGEIQPLHAVRGVLDPQNKLLKRYDNTPAPAQPGDSVAANVISVALQEVVNSGTARQLIID
GLGRLSPAGKTGTSNDGRDSWYAGYTGNHLAVIWVGNDQNEQTGLYGATGAMRVWSSIFSQLPSTRLQVNGKGLDWQSVE
PMGTGTTDANCPGARRFPFVTGFAPPYTACSGAGGAPEAGHNIGWRNWFGLDKQKGDQASPPITPPPDQSTQ
>Mature_791_residues
PGHSPWWGWLIACGLAGLALAMGFLIPYTLYLNQQVTARFGELRWQIPTRVYARPLMLQPGMALDARTLKIELDAASYRE
DNHGELPGTYQQQNGRFTVSSRGYVDVDGAIPASRLIITLNNNQVSALRNADTRKPIRRGRLDPARIATLYGQKQEERRL
VRLEEVPQLLVTGLQVVEDRDFKHHHGIDITSILRAAWVMVRSGGGVRQGASTLTQQLARSGLLGIGKEQTLQRKCNEIL
YALILEARYSKRVILESYLNQVYLGQRGSQAVHGVATGAEFWYGRELGSLTTEQVALLIGLVKGPSYYDPRRFPDRALSR
RNFVLGKLHENKLIDDAEYMRAQASPLGVPKEPGLVAANRFVAYLDLVRRQLAHDYPENVLRGAGMTVLTGMSPSAQAYA
ERGVKTTLSALEKVKGLKRPPLQAGLVLTDVHNGDVLAVVGSRDATQSGFNRAIEAQRQVGSLLKPFVYLLALASPDRWS
LSSWVEDTPVNIPLAHGRIWSPSNSDNISHGTVRLIDALAHSYNQATVRVGMQVGPERVAQLINVLAGLKADANPSLILG
ATDQSPYAMTQLYQFLASGGEIQPLHAVRGVLDPQNKLLKRYDNTPAPAQPGDSVAANVISVALQEVVNSGTARQLIIDG
LGRLSPAGKTGTSNDGRDSWYAGYTGNHLAVIWVGNDQNEQTGLYGATGAMRVWSSIFSQLPSTRLQVNGKGLDWQSVEP
MGTGTTDANCPGARRFPFVTGFAPPYTACSGAGGAPEAGHNIGWRNWFGLDKQKGDQASPPITPPPDQSTQ

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=707, Percent_Identity=35.7850070721358, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI87082258, Length=298, Percent_Identity=32.2147651006711, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1788867, Length=545, Percent_Identity=28.8073394495413, Blast_Score=126, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 86286; Mature: 86155

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGHSPWWGWLIACGLAGLALAMGFLIPYTLYLNQQVTARFGELRWQIPTRVYARPLMLQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEEECCHHHHHCCCEEC
PGMALDARTLKIELDAASYREDNHGELPGTYQQQNGRFTVSSRGYVDVDGAIPASRLIIT
CCCCCCCEEEEEEECHHHCCCCCCCCCCCCEECCCCEEEECCCCEEEECCCCCCEEEEEE
LNNNQVSALRNADTRKPIRRGRLDPARIATLYGQKQEERRLVRLEEVPQLLVTGLQVVED
ECCCCCHHHHCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
RDFKHHHGIDITSILRAAWVMVRSGGGVRQGASTLTQQLARSGLLGIGKEQTLQRKCNEI
CCCCHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LYALILEARYSKRVILESYLNQVYLGQRGSQAVHGVATGAEFWYGRELGSLTTEQVALLI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHCCHHCCCHHHHHHHHH
GLVKGPSYYDPRRFPDRALSRRNFVLGKLHENKLIDDAEYMRAQASPLGVPKEPGLVAAN
HHHCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHH
RFVAYLDLVRRQLAHDYPENVLRGAGMTVLTGMSPSAQAYAERGVKTTLSALEKVKGLKR
HHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHCCCC
PPLQAGLVLTDVHNGDVLAVVGSRDATQSGFNRAIEAQRQVGSLLKPFVYLLALASPDRW
CCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SLSSWVEDTPVNIPLAHGRIWSPSNSDNISHGTVRLIDALAHSYNQATVRVGMQVGPERV
CHHHHCCCCCCCEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHH
AQLINVLAGLKADANPSLILGATDQSPYAMTQLYQFLASGGEIQPLHAVRGVLDPQNKLL
HHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHH
KRYDNTPAPAQPGDSVAANVISVALQEVVNSGTARQLIIDGLGRLSPAGKTGTSNDGRDS
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
WYAGYTGNHLAVIWVGNDQNEQTGLYGATGAMRVWSSIFSQLPSTRLQVNGKGLDWQSVE
EECCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
PMGTGTTDANCPGARRFPFVTGFAPPYTACSGAGGAPEAGHNIGWRNWFGLDKQKGDQAS
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
PPITPPPDQSTQ
CCCCCCCCCCCC
>Mature Secondary Structure 
PGHSPWWGWLIACGLAGLALAMGFLIPYTLYLNQQVTARFGELRWQIPTRVYARPLMLQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHEEEECCHHHHHCCCEEC
PGMALDARTLKIELDAASYREDNHGELPGTYQQQNGRFTVSSRGYVDVDGAIPASRLIIT
CCCCCCCEEEEEEECHHHCCCCCCCCCCCCEECCCCEEEECCCCEEEECCCCCCEEEEEE
LNNNQVSALRNADTRKPIRRGRLDPARIATLYGQKQEERRLVRLEEVPQLLVTGLQVVED
ECCCCCHHHHCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
RDFKHHHGIDITSILRAAWVMVRSGGGVRQGASTLTQQLARSGLLGIGKEQTLQRKCNEI
CCCCHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LYALILEARYSKRVILESYLNQVYLGQRGSQAVHGVATGAEFWYGRELGSLTTEQVALLI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHCCHHCCCHHHHHHHHH
GLVKGPSYYDPRRFPDRALSRRNFVLGKLHENKLIDDAEYMRAQASPLGVPKEPGLVAAN
HHHCCCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHH
RFVAYLDLVRRQLAHDYPENVLRGAGMTVLTGMSPSAQAYAERGVKTTLSALEKVKGLKR
HHHHHHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHCCCC
PPLQAGLVLTDVHNGDVLAVVGSRDATQSGFNRAIEAQRQVGSLLKPFVYLLALASPDRW
CCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SLSSWVEDTPVNIPLAHGRIWSPSNSDNISHGTVRLIDALAHSYNQATVRVGMQVGPERV
CHHHHCCCCCCCEEECCCEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHH
AQLINVLAGLKADANPSLILGATDQSPYAMTQLYQFLASGGEIQPLHAVRGVLDPQNKLL
HHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHH
KRYDNTPAPAQPGDSVAANVISVALQEVVNSGTARQLIIDGLGRLSPAGKTGTSNDGRDS
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
WYAGYTGNHLAVIWVGNDQNEQTGLYGATGAMRVWSSIFSQLPSTRLQVNGKGLDWQSVE
EECCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
PMGTGTTDANCPGARRFPFVTGFAPPYTACSGAGGAPEAGHNIGWRNWFGLDKQKGDQAS
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
PPITPPPDQSTQ
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]