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The map label for this gene is btuB [H]

Identifier: 182682323

GI number: 182682323

Start: 2015737

End: 2018520

Strand: Reverse

Name: btuB [H]

Synonym: XfasM23_1806

Alternate gene names: 182682323

Gene position: 2018520-2015737 (Counterclockwise)

Preceding gene: 182682325

Following gene: 182682321

Centisome position: 79.6

GC content: 51.11

Gene sequence:

>2784_bases
GTGACGCATGTCGCTATTGATTCACATCGGTATATTCCCCGCAATCTTCTGTGTGCATCCATTTCTGGTGTACTCCTCGT
TGCTGCCGTCAGCGTTCAGGCACAAGAAACTAACGCTGCAAGCAATTTAGAACGTATTACCGTCACAGGTTCTAATATCC
CCCGCACGGATACCGAGACCGCGTCGCCAGTCCAGATTGTCACTCGTGAGGACATCGCGCGTTCCGGTCGTGCCACAGTT
GCAGAGTATCTACAGACTTTAACTTCTGATGGCCAGGGTTCGATACCCAAGAATTTTGGTAACGGTTTCGCAATGGGTGG
GAGTGGTATCTCGTTGCGTGGTTTGGGTGCCGGCTCCACTCTGGTGTTGATTAACGGTCGTCGTATCGCTTCCTACGGGT
TGGCCGACGATGGTCAGAAGTTGTTCGCTGATTTGAGTAGCATCCCGATGGATGCGGTTGAGCGTGTCGAAGTTCTGAAG
GACGGTGCGTCCTCAATTTACGGATCTGACGCGATCGCCGGTGTAGTCAATATTATTTTGCGTAATGACTTCCAGGGTGC
TCTTGTGAGTGCTTCATATGGTCTTTCTGGTTATAGGGATGGCCATACCCCAAAGGCCACAATGACCGTTGGCGTTGGTA
ATTTGGCATCCGATGGCTGGAATGCGCTTGTGAGCGTGGATGTTGGCAAGAGTGATGGGATTATGACGAGTGACCGGAAA
GATCGTAAGTGGATCGGTACTGGTGATACTCGTCCCTGGGGCTACGACCTCGCAAACAATCAGCAGTTTCTTCGGGGCGC
AATCACTGGCAAAGGTACTATTGCCGACTCTTCGCCGGTAGGGGCAGTCCGTAACCCGTCTAATAGTCAGTTCAGTTCGC
TACCAGGTTGTTCAAAATTTTCCAGTATTTCGCCACAGGATCCAGGAGGCGGCTGTTTATGGGATGCGGCTCAGTTCCGT
AGCCTGACTCCGGATGAGAAATACGCCAACGTCTTCACTCGTGGCACTTTCACTCTGTTCGATACCACTGAGTTATATAC
CGAGCTGAGCTACTCTAAGAAAAAGTCCGAGTTCAGTAATTTACCATCTCCCGTTTCAGGTGCTTGGGGCTCTCCAGGCG
GTGCGGTCAATGCCAGTAGTGGTGACGGTGCTACGGTCTTGGCCCCCGGGCATCCGGACAACCCGTACAACGTACCTGCG
CGCCTGCGTTATGCAGCGTTTGACGTTGGTCCTCGTGTGAAAGAAAACACAAATGACTTTATGCGTGTTGTGTTGGGGAT
CAAGGGAAGTGTTGCCAGTTGGGATTATGATGTCAGTGTCTTGCATTCTCAGTCACAAATGGACAGGAAGTTCACTGGTT
ACTTGCGCTATAGCTCTGTGCGTTGTGTGCTTGGAAACTTAGCCTGCCCTGCTGGTGTATGGAATATTGGTAATGGCTCC
GAAACTAACCCGCGGTCGCTGTACGATTTCATTTCACCTGTACTTAGCGCGCAGGCCAAAACTAAGCTGGATGTGTTTGA
TGCAAGGGTCTCGCGTGCATTGGCTGACTTATCCGGTGGTGCTATGGGCTTGGCGATGGGTGTGGAATACCGCCGCGATC
AAGCTTCGTTAACTCCAATGACTTATACCGATATCGGCGACATCATTGGTGCGGGGTATTCGGCCTATAGTGGTGAAGAA
AAGGTGAGTGCTGCGTATGCTGAATTAGCCGCGCCGTTTACCAAAGCTTTTGAGGCGACTGTGGCTGGTCGTGTTGACCA
CTACAGTAGTGGCAATACCGCCATTACTCCGAAGTTGGGTGTGAAGTGGAAACCTTTTGATTGGTTTGCGCTACGTGGCA
CTTATGCCCAGGGTTTCCGTGCACCGAATACTGCTGAGTCCGGTAAAGGTGGTTTAGCTGCGTTTGCGGGCATAACTGAC
CCAGTGCGTTGTGCCGCTGCAGGGGGGAGTGGCGCTGAATGTGCGCCTCGTCTCGTTTCAGTTATCACTAGCCCGAATCC
AGATCTTAAGCCTGAGAAGTCAAATAGTTATACGCTTGGCATGGTGTTCCAGCCGACTAGCAGTACATCTATGACGGTGG
ATGGCTGGCAAATCAAGCGTACTAAAGAGATTGCTCAGATCGGCACAGTTGATGCGATCAATGCAGGTTATGTAATACGT
GACGATAACCTGTTGAAAGGTGTTGCCGGCACTGGTCAGTTGCTTGGGGTCAAGGCCAAATACGTCAATCTCAACGCCAC
TACAGCACGTGGTGTGGATACTGACGTACAGCATCGTATTGCCATTGGTGGACTAGGTGATTTGATCCTGGATTTGCAGT
GGAGTCGCATGCTTAAGTTTCAGCGCGAGGAAGCTAATGGCAACATCAGTGAGTATGCCGGTACGCATGGTAATTGCGAT
GTAAGCAATTGCATTGGTACTCCGAAGGACCGGATTAATTTTGGGGCGACTTGGAAATTGAACAACATCAGTGTGAGTGC
GTTAGGCCATTACCGTGGCCCGATGGAAAATAGGCTGTTTAAAGGTGGTGCTTGCGTCAGCCATTTTGCTGATGGAAGTG
ATGCTCCAAATCATTGCCGTTTGAACGCGTTTACCTCCGTGGACTTGTCAGCAAATTGGCAGGCCACTGAAGCACTGGCA
GTGTTTGGTTCAGTGCAGAACCTGTTTGACCGAATCGCTCCACTTGATCCGTTGACCGGTGGGGCACTGAACTATAATCC
ATTGGATTCTGCGGGTGCGGTTGGTCGCTTCTTCACAGTTGGCGCTCGCTACGCCTTTAAGTAA

Upstream 100 bases:

>100_bases
CATCTAACTCTTGATTTACGAATGCACTAACGACGATGCTCGCTACCAGGCCAGAAAAAAGGCCGATTTTACTATATTTT
TACCGTTTCAGGAGAAAAAT

Downstream 100 bases:

>100_bases
TTGGTGGTTTGTTAGACCCGAAAACCCCTCGGTCATTTAGACTGGGGGTTTTTTGTGCCGATGTGTATAAGGCACATTAG
TCCTACTGTGTTGAGTGGTA

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 927; Mature: 926

Protein sequence:

>927_residues
MTHVAIDSHRYIPRNLLCASISGVLLVAAVSVQAQETNAASNLERITVTGSNIPRTDTETASPVQIVTREDIARSGRATV
AEYLQTLTSDGQGSIPKNFGNGFAMGGSGISLRGLGAGSTLVLINGRRIASYGLADDGQKLFADLSSIPMDAVERVEVLK
DGASSIYGSDAIAGVVNIILRNDFQGALVSASYGLSGYRDGHTPKATMTVGVGNLASDGWNALVSVDVGKSDGIMTSDRK
DRKWIGTGDTRPWGYDLANNQQFLRGAITGKGTIADSSPVGAVRNPSNSQFSSLPGCSKFSSISPQDPGGGCLWDAAQFR
SLTPDEKYANVFTRGTFTLFDTTELYTELSYSKKKSEFSNLPSPVSGAWGSPGGAVNASSGDGATVLAPGHPDNPYNVPA
RLRYAAFDVGPRVKENTNDFMRVVLGIKGSVASWDYDVSVLHSQSQMDRKFTGYLRYSSVRCVLGNLACPAGVWNIGNGS
ETNPRSLYDFISPVLSAQAKTKLDVFDARVSRALADLSGGAMGLAMGVEYRRDQASLTPMTYTDIGDIIGAGYSAYSGEE
KVSAAYAELAAPFTKAFEATVAGRVDHYSSGNTAITPKLGVKWKPFDWFALRGTYAQGFRAPNTAESGKGGLAAFAGITD
PVRCAAAGGSGAECAPRLVSVITSPNPDLKPEKSNSYTLGMVFQPTSSTSMTVDGWQIKRTKEIAQIGTVDAINAGYVIR
DDNLLKGVAGTGQLLGVKAKYVNLNATTARGVDTDVQHRIAIGGLGDLILDLQWSRMLKFQREEANGNISEYAGTHGNCD
VSNCIGTPKDRINFGATWKLNNISVSALGHYRGPMENRLFKGGACVSHFADGSDAPNHCRLNAFTSVDLSANWQATEALA
VFGSVQNLFDRIAPLDPLTGGALNYNPLDSAGAVGRFFTVGARYAFK

Sequences:

>Translated_927_residues
MTHVAIDSHRYIPRNLLCASISGVLLVAAVSVQAQETNAASNLERITVTGSNIPRTDTETASPVQIVTREDIARSGRATV
AEYLQTLTSDGQGSIPKNFGNGFAMGGSGISLRGLGAGSTLVLINGRRIASYGLADDGQKLFADLSSIPMDAVERVEVLK
DGASSIYGSDAIAGVVNIILRNDFQGALVSASYGLSGYRDGHTPKATMTVGVGNLASDGWNALVSVDVGKSDGIMTSDRK
DRKWIGTGDTRPWGYDLANNQQFLRGAITGKGTIADSSPVGAVRNPSNSQFSSLPGCSKFSSISPQDPGGGCLWDAAQFR
SLTPDEKYANVFTRGTFTLFDTTELYTELSYSKKKSEFSNLPSPVSGAWGSPGGAVNASSGDGATVLAPGHPDNPYNVPA
RLRYAAFDVGPRVKENTNDFMRVVLGIKGSVASWDYDVSVLHSQSQMDRKFTGYLRYSSVRCVLGNLACPAGVWNIGNGS
ETNPRSLYDFISPVLSAQAKTKLDVFDARVSRALADLSGGAMGLAMGVEYRRDQASLTPMTYTDIGDIIGAGYSAYSGEE
KVSAAYAELAAPFTKAFEATVAGRVDHYSSGNTAITPKLGVKWKPFDWFALRGTYAQGFRAPNTAESGKGGLAAFAGITD
PVRCAAAGGSGAECAPRLVSVITSPNPDLKPEKSNSYTLGMVFQPTSSTSMTVDGWQIKRTKEIAQIGTVDAINAGYVIR
DDNLLKGVAGTGQLLGVKAKYVNLNATTARGVDTDVQHRIAIGGLGDLILDLQWSRMLKFQREEANGNISEYAGTHGNCD
VSNCIGTPKDRINFGATWKLNNISVSALGHYRGPMENRLFKGGACVSHFADGSDAPNHCRLNAFTSVDLSANWQATEALA
VFGSVQNLFDRIAPLDPLTGGALNYNPLDSAGAVGRFFTVGARYAFK
>Mature_926_residues
THVAIDSHRYIPRNLLCASISGVLLVAAVSVQAQETNAASNLERITVTGSNIPRTDTETASPVQIVTREDIARSGRATVA
EYLQTLTSDGQGSIPKNFGNGFAMGGSGISLRGLGAGSTLVLINGRRIASYGLADDGQKLFADLSSIPMDAVERVEVLKD
GASSIYGSDAIAGVVNIILRNDFQGALVSASYGLSGYRDGHTPKATMTVGVGNLASDGWNALVSVDVGKSDGIMTSDRKD
RKWIGTGDTRPWGYDLANNQQFLRGAITGKGTIADSSPVGAVRNPSNSQFSSLPGCSKFSSISPQDPGGGCLWDAAQFRS
LTPDEKYANVFTRGTFTLFDTTELYTELSYSKKKSEFSNLPSPVSGAWGSPGGAVNASSGDGATVLAPGHPDNPYNVPAR
LRYAAFDVGPRVKENTNDFMRVVLGIKGSVASWDYDVSVLHSQSQMDRKFTGYLRYSSVRCVLGNLACPAGVWNIGNGSE
TNPRSLYDFISPVLSAQAKTKLDVFDARVSRALADLSGGAMGLAMGVEYRRDQASLTPMTYTDIGDIIGAGYSAYSGEEK
VSAAYAELAAPFTKAFEATVAGRVDHYSSGNTAITPKLGVKWKPFDWFALRGTYAQGFRAPNTAESGKGGLAAFAGITDP
VRCAAAGGSGAECAPRLVSVITSPNPDLKPEKSNSYTLGMVFQPTSSTSMTVDGWQIKRTKEIAQIGTVDAINAGYVIRD
DNLLKGVAGTGQLLGVKAKYVNLNATTARGVDTDVQHRIAIGGLGDLILDLQWSRMLKFQREEANGNISEYAGTHGNCDV
SNCIGTPKDRINFGATWKLNNISVSALGHYRGPMENRLFKGGACVSHFADGSDAPNHCRLNAFTSVDLSANWQATEALAV
FGSVQNLFDRIAPLDPLTGGALNYNPLDSAGAVGRFFTVGARYAFK

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=182, Percent_Identity=35.1648351648352, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788478, Length=162, Percent_Identity=29.6296296296296, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 98089; Mature: 97958

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00104 EPSP_SYNTHASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVAIDSHRYIPRNLLCASISGVLLVAAVSVQAQETNAASNLERITVTGSNIPRTDTET
CCEEEECCCCCCCHHHHHHHHHHEEEEHHHHEECCCCCCCCCCEEEEEECCCCCCCCCCC
ASPVQIVTREDIARSGRATVAEYLQTLTSDGQGSIPKNFGNGFAMGGSGISLRGLGAGST
CCCEEEEEHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEEECCCCCE
LVLINGRRIASYGLADDGQKLFADLSSIPMDAVERVEVLKDGASSIYGSDAIAGVVNIIL
EEEEECCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHH
RNDFQGALVSASYGLSGYRDGHTPKATMTVGVGNLASDGWNALVSVDVGKSDGIMTSDRK
HCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCC
DRKWIGTGDTRPWGYDLANNQQFLRGAITGKGTIADSSPVGAVRNPSNSQFSSLPGCSKF
CCCEECCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCHHCCCCCCHH
SSISPQDPGGGCLWDAAQFRSLTPDEKYANVFTRGTFTLFDTTELYTELSYSKKKSEFSN
CCCCCCCCCCCEEECHHHHCCCCCCHHHHHEEECCEEEEEEHHHHHHHHHHHHHHHHHHC
LPSPVSGAWGSPGGAVNASSGDGATVLAPGHPDNPYNVPARLRYAAFDVGPRVKENTNDF
CCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCHHHH
MRVVLGIKGSVASWDYDVSVLHSQSQMDRKFTGYLRYSSVRCVLGNLACPAGVWNIGNGS
HHHHHHCCCCCCCCCCCCEEEECHHHHHHHHEEEEEECCCEEEECCCCCCCCCCCCCCCC
ETNPRSLYDFISPVLSAQAKTKLDVFDARVSRALADLSGGAMGLAMGVEYRRDQASLTPM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCE
TYTDIGDIIGAGYSAYSGEEKVSAAYAELAAPFTKAFEATVAGRVDHYSSGNTAITPKLG
EHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEECCCCC
VKWKPFDWFALRGTYAQGFRAPNTAESGKGGLAAFAGITDPVRCAAAGGSGAECAPRLVS
CEECCCCEEEEECCHHHCCCCCCCCCCCCCCEEEECCCCCCHHEEECCCCCCHHHHHHHH
VITSPNPDLKPEKSNSYTLGMVFQPTSSTSMTVDGWQIKRTKEIAQIGTVDAINAGYVIR
HHCCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCEEEHHHHHHHHHCCEEECCCCEEEE
DDNLLKGVAGTGQLLGVKAKYVNLNATTARGVDTDVQHRIAIGGLGDLILDLQWSRMLKF
CCCHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHEEEECCCCHHHHHCCHHHHHHH
QREEANGNISEYAGTHGNCDVSNCIGTPKDRINFGATWKLNNISVSALGHYRGPMENRLF
HHHHCCCCHHHHCCCCCCCCHHHCCCCCHHHCCCCEEEEECCEEEEEECCCCCCHHHHEE
KGGACVSHFADGSDAPNHCRLNAFTSVDLSANWQATEALAVFGSVQNLFDRIAPLDPLTG
CCCCHHHHCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
GALNYNPLDSAGAVGRFFTVGARYAFK
CCCCCCCCCCCCCCCHHEEECCEEECC
>Mature Secondary Structure 
THVAIDSHRYIPRNLLCASISGVLLVAAVSVQAQETNAASNLERITVTGSNIPRTDTET
CEEEECCCCCCCHHHHHHHHHHEEEEHHHHEECCCCCCCCCCEEEEEECCCCCCCCCCC
ASPVQIVTREDIARSGRATVAEYLQTLTSDGQGSIPKNFGNGFAMGGSGISLRGLGAGST
CCCEEEEEHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEEECCCCCE
LVLINGRRIASYGLADDGQKLFADLSSIPMDAVERVEVLKDGASSIYGSDAIAGVVNIIL
EEEEECCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHH
RNDFQGALVSASYGLSGYRDGHTPKATMTVGVGNLASDGWNALVSVDVGKSDGIMTSDRK
HCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCC
DRKWIGTGDTRPWGYDLANNQQFLRGAITGKGTIADSSPVGAVRNPSNSQFSSLPGCSKF
CCCEECCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCHHCCCCCCHH
SSISPQDPGGGCLWDAAQFRSLTPDEKYANVFTRGTFTLFDTTELYTELSYSKKKSEFSN
CCCCCCCCCCCEEECHHHHCCCCCCHHHHHEEECCEEEEEEHHHHHHHHHHHHHHHHHHC
LPSPVSGAWGSPGGAVNASSGDGATVLAPGHPDNPYNVPARLRYAAFDVGPRVKENTNDF
CCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCHHHH
MRVVLGIKGSVASWDYDVSVLHSQSQMDRKFTGYLRYSSVRCVLGNLACPAGVWNIGNGS
HHHHHHCCCCCCCCCCCCEEEECHHHHHHHHEEEEEECCCEEEECCCCCCCCCCCCCCCC
ETNPRSLYDFISPVLSAQAKTKLDVFDARVSRALADLSGGAMGLAMGVEYRRDQASLTPM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCE
TYTDIGDIIGAGYSAYSGEEKVSAAYAELAAPFTKAFEATVAGRVDHYSSGNTAITPKLG
EHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEECCCCC
VKWKPFDWFALRGTYAQGFRAPNTAESGKGGLAAFAGITDPVRCAAAGGSGAECAPRLVS
CEECCCCEEEEECCHHHCCCCCCCCCCCCCCEEEECCCCCCHHEEECCCCCCHHHHHHHH
VITSPNPDLKPEKSNSYTLGMVFQPTSSTSMTVDGWQIKRTKEIAQIGTVDAINAGYVIR
HHCCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCEEEHHHHHHHHHCCEEECCCCEEEE
DDNLLKGVAGTGQLLGVKAKYVNLNATTARGVDTDVQHRIAIGGLGDLILDLQWSRMLKF
CCCHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHEEEECCCCHHHHHCCHHHHHHH
QREEANGNISEYAGTHGNCDVSNCIGTPKDRINFGATWKLNNISVSALGHYRGPMENRLF
HHHHCCCCHHHHCCCCCCCCHHHCCCCCHHHCCCCEEEEECCEEEEEECCCCCCHHHHEE
KGGACVSHFADGSDAPNHCRLNAFTSVDLSANWQATEALAVFGSVQNLFDRIAPLDPLTG
CCCCHHHHCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
GALNYNPLDSAGAVGRFFTVGARYAFK
CCCCCCCCCCCCCCCHHEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA