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The map label for this gene is dnaG

Identifier: 182682257

GI number: 182682257

Start: 1921371

End: 1923104

Strand: Direct

Name: dnaG

Synonym: XfasM23_1739

Alternate gene names: 182682257

Gene position: 1921371-1923104 (Clockwise)

Preceding gene: 182682256

Following gene: 182682266

Centisome position: 75.77

GC content: 55.59

Gene sequence:

>1734_bases
ATGGCCCGCATCCCTGATGCATTCATTGATGAACTACTCGCCCGTACCGATATCGTGGAGGTAGTAGGCAGCCGCCTACC
GCTGAAGCGTCAAGGCAAAGAATACGCATCGCGGTGCCCATTCCACGACGAACGCTCCGCCTCCTTCTATGTCTCTCCGA
CCAAGCAGTTCTACCACTGCTTTGGTTGTGGCGCACACGGCACCGCCATCAACTTTCTAATGAACTACGACCGCCTCGAA
TTCCTTGATGCCGTCGACGAACTGGCCAAACGCGCCGGAATGGAAATCCCCCGCGAAACCCAGCAAAAACACGCACACCA
GGACGACAGCCGCCCACTCTACGCCGCACTAGAGGCTGCATCTCACTTTTTCCAAAAACACCTAGAAAACAACCCCAAAG
TCCAGGCATACCTGAATGAACGCGGTGTTGATATCAACACCCGCACACGCTTCCAGATTGGTTACGCACCCGACGGATAT
AGCGTACTGAAAGACACCCTAGGGACCGACGAACGACGCATCACCCTATTAGATCGGGCAGGCATGCTCTCCAAAAACGA
CCGCGACCACGTCTACGACAAATTCCGCGACCGAGTCATGTTCCCGATCTTTGACCGACGCGGCCGCGTCATCGCCTTTG
GCGGCCGCGTCCTGGACAAAGATAAAAGCCCGAAATACCTCAACTCCCCGGAAACCACCTTATTTCACAAAAGCCGCGAA
CTGTACGGCCTCTGGCAAGTACGCCAAGCACACCAGAACATCGAACGACTGATCGTCGTCGAAGGCTACATGGATGTGAT
CTCACTCTTCCAATTTGGCATCACCCAAGCCGTCGCCACACTTGGCACAGCAACCACCGTAGAACACGCCGAACTCCTAT
TTCGCAATACCCCGGACGTGTACTTCTGCTTTGACGGCGACAACGCCGGCCGGAAAGCCGGTTGGCGCGCACTTGAATCG
ATCCTGCCACGCATGAAAGAAGGACGACAAGCCTTCTTCCTCTTCCTGCCCGATGGCGAAGATCCAGACACCATCGTCCG
TAAAGAAGGCAAAAACGCATTCAACGAACGCCTCAAACAAGCCATCCCACTGTCACAATTCTTCTTCGACACACTCTCCA
GCGACATCGCCCTGCATACCCTGGACGGCAAAGCACGCCTGGCCGAACGCGCCCGCCCACTGCTCATGAAAATCCCCGAA
GGCGCATTTAACGACCTCATGCGGCAACGCCTGGCACACCTCACCGGTGTCAACATCGCCCACCCCGGACCAGCCACTGC
CGCCACATCACGGCCAGCGACACGCCTTACTGCACAGAAACGCAACCTCATCCACACAACAATCGCAATCATCCTGCAAC
AGCCCTCCCTGGCAATGACCCTGAACACCCCATACCCTCTTGCCATTAACGACCTAAAACAACCAGGCATCGCCCTACTA
CTAGAATTACTCGACCTGATACACCAGCGCCCGGAGATCACCACAGGCGCCCTGCTAGAACACTTCACCGAACGCGAAGA
ATACGTCCTACTACAGACACTAGCCACACAAACCATGCCAGGTGACACCGCCACCTGGACCCAAGAATTACAAGGCGCGA
TGACACAAATGCAGAAACAGCTACTCCAACAACGCCTGAACGCACTCCACACCAAACAACTGACCCAGCCCCTGGACGAC
ATCGACAAGTACGAACTGCGTGAACTGCTCAAAAACCGCACCACACTACGGTGA

Upstream 100 bases:

>100_bases
CAACGAATGCCCCCCAACCACACAACCCTCCCCCTACACCACCGACAGCTCCCCTCCCCCAATACAACACCACACTTGAC
AGACCGCCTACACTACTAAA

Downstream 100 bases:

>100_bases
AACATCCCACAACCGTCCTACATCCCTACGCAACAACACCCAACCAACACGGATGACCCAACCCATTTGATACAACCAAC
ATCCCGCGTGCCTTGAAACA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MARIPDAFIDELLARTDIVEVVGSRLPLKRQGKEYASRCPFHDERSASFYVSPTKQFYHCFGCGAHGTAINFLMNYDRLE
FLDAVDELAKRAGMEIPRETQQKHAHQDDSRPLYAALEAASHFFQKHLENNPKVQAYLNERGVDINTRTRFQIGYAPDGY
SVLKDTLGTDERRITLLDRAGMLSKNDRDHVYDKFRDRVMFPIFDRRGRVIAFGGRVLDKDKSPKYLNSPETTLFHKSRE
LYGLWQVRQAHQNIERLIVVEGYMDVISLFQFGITQAVATLGTATTVEHAELLFRNTPDVYFCFDGDNAGRKAGWRALES
ILPRMKEGRQAFFLFLPDGEDPDTIVRKEGKNAFNERLKQAIPLSQFFFDTLSSDIALHTLDGKARLAERARPLLMKIPE
GAFNDLMRQRLAHLTGVNIAHPGPATAATSRPATRLTAQKRNLIHTTIAIILQQPSLAMTLNTPYPLAINDLKQPGIALL
LELLDLIHQRPEITTGALLEHFTEREEYVLLQTLATQTMPGDTATWTQELQGAMTQMQKQLLQQRLNALHTKQLTQPLDD
IDKYELRELLKNRTTLR

Sequences:

>Translated_577_residues
MARIPDAFIDELLARTDIVEVVGSRLPLKRQGKEYASRCPFHDERSASFYVSPTKQFYHCFGCGAHGTAINFLMNYDRLE
FLDAVDELAKRAGMEIPRETQQKHAHQDDSRPLYAALEAASHFFQKHLENNPKVQAYLNERGVDINTRTRFQIGYAPDGY
SVLKDTLGTDERRITLLDRAGMLSKNDRDHVYDKFRDRVMFPIFDRRGRVIAFGGRVLDKDKSPKYLNSPETTLFHKSRE
LYGLWQVRQAHQNIERLIVVEGYMDVISLFQFGITQAVATLGTATTVEHAELLFRNTPDVYFCFDGDNAGRKAGWRALES
ILPRMKEGRQAFFLFLPDGEDPDTIVRKEGKNAFNERLKQAIPLSQFFFDTLSSDIALHTLDGKARLAERARPLLMKIPE
GAFNDLMRQRLAHLTGVNIAHPGPATAATSRPATRLTAQKRNLIHTTIAIILQQPSLAMTLNTPYPLAINDLKQPGIALL
LELLDLIHQRPEITTGALLEHFTEREEYVLLQTLATQTMPGDTATWTQELQGAMTQMQKQLLQQRLNALHTKQLTQPLDD
IDKYELRELLKNRTTLR
>Mature_576_residues
ARIPDAFIDELLARTDIVEVVGSRLPLKRQGKEYASRCPFHDERSASFYVSPTKQFYHCFGCGAHGTAINFLMNYDRLEF
LDAVDELAKRAGMEIPRETQQKHAHQDDSRPLYAALEAASHFFQKHLENNPKVQAYLNERGVDINTRTRFQIGYAPDGYS
VLKDTLGTDERRITLLDRAGMLSKNDRDHVYDKFRDRVMFPIFDRRGRVIAFGGRVLDKDKSPKYLNSPETTLFHKSREL
YGLWQVRQAHQNIERLIVVEGYMDVISLFQFGITQAVATLGTATTVEHAELLFRNTPDVYFCFDGDNAGRKAGWRALESI
LPRMKEGRQAFFLFLPDGEDPDTIVRKEGKNAFNERLKQAIPLSQFFFDTLSSDIALHTLDGKARLAERARPLLMKIPEG
AFNDLMRQRLAHLTGVNIAHPGPATAATSRPATRLTAQKRNLIHTTIAIILQQPSLAMTLNTPYPLAINDLKQPGIALLL
ELLDLIHQRPEITTGALLEHFTEREEYVLLQTLATQTMPGDTATWTQELQGAMTQMQKQLLQQRLNALHTKQLTQPLDDI
DKYELRELLKNRTTLR

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=581, Percent_Identity=46.1273666092943, Blast_Score=495, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65787; Mature: 65656

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIPDAFIDELLARTDIVEVVGSRLPLKRQGKEYASRCPFHDERSASFYVSPTKQFYHC
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCCCCCCCCEEEECCHHHHHHH
FGCGAHGTAINFLMNYDRLEFLDAVDELAKRAGMEIPRETQQKHAHQDDSRPLYAALEAA
HCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHH
SHFFQKHLENNPKVQAYLNERGVDINTRTRFQIGYAPDGYSVLKDTLGTDERRITLLDRA
HHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEECC
GMLSKNDRDHVYDKFRDRVMFPIFDRRGRVIAFGGRVLDKDKSPKYLNSPETTLFHKSRE
CCCCCCCHHHHHHHHHHCEECEEECCCCCEEEECCEEECCCCCCCCCCCCCHHHHHCCHH
LYGLWQVRQAHQNIERLIVVEGYMDVISLFQFGITQAVATLGTATTVEHAELLFRNTPDV
HHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCE
YFCFDGDNAGRKAGWRALESILPRMKEGRQAFFLFLPDGEDPDTIVRKEGKNAFNERLKQ
EEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHH
AIPLSQFFFDTLSSDIALHTLDGKARLAERARPLLMKIPEGAFNDLMRQRLAHLTGVNIA
HCCHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCC
HPGPATAATSRPATRLTAQKRNLIHTTIAIILQQPSLAMTLNTPYPLAINDLKQPGIALL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCHHHHH
LELLDLIHQRPEITTGALLEHFTEREEYVLLQTLATQTMPGDTATWTQELQGAMTQMQKQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LLQQRLNALHTKQLTQPLDDIDKYELRELLKNRTTLR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARIPDAFIDELLARTDIVEVVGSRLPLKRQGKEYASRCPFHDERSASFYVSPTKQFYHC
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCCCCCCCCEEEECCHHHHHHH
FGCGAHGTAINFLMNYDRLEFLDAVDELAKRAGMEIPRETQQKHAHQDDSRPLYAALEAA
HCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHH
SHFFQKHLENNPKVQAYLNERGVDINTRTRFQIGYAPDGYSVLKDTLGTDERRITLLDRA
HHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEECC
GMLSKNDRDHVYDKFRDRVMFPIFDRRGRVIAFGGRVLDKDKSPKYLNSPETTLFHKSRE
CCCCCCCHHHHHHHHHHCEECEEECCCCCEEEECCEEECCCCCCCCCCCCCHHHHHCCHH
LYGLWQVRQAHQNIERLIVVEGYMDVISLFQFGITQAVATLGTATTVEHAELLFRNTPDV
HHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCE
YFCFDGDNAGRKAGWRALESILPRMKEGRQAFFLFLPDGEDPDTIVRKEGKNAFNERLKQ
EEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHH
AIPLSQFFFDTLSSDIALHTLDGKARLAERARPLLMKIPEGAFNDLMRQRLAHLTGVNIA
HCCHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCCCCC
HPGPATAATSRPATRLTAQKRNLIHTTIAIILQQPSLAMTLNTPYPLAINDLKQPGIALL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCHHHHH
LELLDLIHQRPEITTGALLEHFTEREEYVLLQTLATQTMPGDTATWTQELQGAMTQMQKQ
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LLQQRLNALHTKQLTQPLDDIDKYELRELLKNRTTLR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]