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The map label for this gene is proS

Identifier: 182682245

GI number: 182682245

Start: 1908506

End: 1910200

Strand: Reverse

Name: proS

Synonym: XfasM23_1727

Alternate gene names: 182682245

Gene position: 1910200-1908506 (Counterclockwise)

Preceding gene: 182682246

Following gene: 182682244

Centisome position: 75.33

GC content: 50.21

Gene sequence:

>1695_bases
ATGCGTTTGTCTGAGTTTCATCTGCATACCACCAAGGAAATCCCCGCTGATGCCGAGTTGGTGAGTCACCGGCTGATGTT
GCGTGCCGGGATGATTCGTAAATTGGCTTCTGGTCTGTATACATGGTCGCCTTTGGGATTGCGTGTTTTGCGTAAAGTGG
AAGCAATCGTGCGCGATGAGATGAATCGTGCCGGTGCGGTAGAAATGTTGTTACCCACAATTCAGCCTCGTGAATTGTGG
GAAGAATCCGAACGCTGGGAGAAGTTTGGTAGCCAGTTACTCAAGATCAAGGACCGTAAACAAGCCGAGTACTGTTACAG
CCCCACTGCAGAAGAGGCAGTGACTGATTACGTACGACAGGAGTTGACCAGTTATAAACAGTTGCCGGTCAATCTTTACC
AAATTCAAACGAAGTTCCGTGATGAGATTAGACCTCGTTTCGGGGTGATGCGTGCGCGTGAATTTGTGATGAAGGATGCT
TACTCGTTCCATCTGAACGATGCTGATTTGGTACGTGAGTACGAAAATATGAGGGCGACGTATACACGTATCTTTACCAG
GCTGGGGTTGGAGTTCCGTGCAGTGCAGGCTGATTCTGGTGCGATTGGCGGTGATGCTTCGCAGGAGTTTCACGTGATTG
CTGATTCTGGAGAAGATGTATTGGCTTTTTCTACGGGATCGGACTATGCGGCCAATATCGAGGCTGCCATTGCTGCTACG
CCTGGTCCGCGCTTGACCGCAAACGAAACGTTACAGAAAGTCAGCACTCCGACGCAGAAGCGTTGTGAAGATGTTGCTGC
TCTGTTGGATATTCCACTGCAGCGTGTGGTGAAGTCTATCGCGGTGATGACCGATAGCGGCTTTTTTCTGGCCTTGTTGC
GAGGCGATCATACGCTTAACGATATTAAATTGAGCAAGCTACCGGGCTTGGCCAACTTTCGTTTGGCTAATGAGGTCGAA
ATAGCCAGACATCTGGGGAGTGAGCCGGGTTTTCTTGGTCCGGTTTGCCCAGGTATGCCGATCCGCATTATTGCTGATTG
TGAGGTTGCGGTGATGGCTGACTTTGTGGTCGGTGCTAACGAGGTTGGCTTTCATTTGGTTGGAGTGAATTGGGGTCGAG
ATTTGCCTGAGCCGGAAGTTGTGGCTGACATCCGTAATGTGATCGAAGGGGATCGCGCTGTCGATGGTGGGAAGATCTGT
ATTGCGCGCGGTATCGAAGTCGGTCATGTGTTCCAGCTTGGTCGTAAGTATGCCGAAGCAATGAAAGCGACGGTGCTGGA
TGAGTATGGCAAAGCTGTCACCATGACGATGGGTTGTTATGGCATCGGTGTGTCACGGATCGTTGCTGCCGCAATCGAAC
AGAACCATGATGTCGCTGGCATCATTTGGCCCGCTCCCATTGCACCATGGCAGGTAGCGGTTTGTGTGATTAATCCAAAA
AAAGATCCGGTCATTACTGCCGCTGCAGAAATGCTTCTTGCTGAGTTGCAGTCAGCGGATGTTGATACGGTATTAGATGA
TCGTGGTTTGCGTCCTGGCGTGATGTTTGCGGACATGGAATTAATCGGTATTCCTCACCGTATTGTGGTTTCCGAACGTG
GATTAGCTGCTGGTACTTATGAATATCGCGCACGTCGTACTGCAATGGTTGAGAATCTGGATAAGACGACTTTGCTGACG
CGAATAAAAGCCTGA

Upstream 100 bases:

>100_bases
CACACCGCCACACTGAGTATTTCTTCAATGTGGCTTGATTTGCCTGCTGCTTAGGAATCGCTGGCAGAATACGGGGTTTA
TTTTCCTTGGGGAGCTTCCA

Downstream 100 bases:

>100_bases
TTGATTTGTGTTATTGAAATATGAATGTTTCATTGTAACTGTTGCTATGTGTTTGTCAGTTTCAAACCGAGTGATTATAA
TGGCCGACGCTTTAAGTACA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MRLSEFHLHTTKEIPADAELVSHRLMLRAGMIRKLASGLYTWSPLGLRVLRKVEAIVRDEMNRAGAVEMLLPTIQPRELW
EESERWEKFGSQLLKIKDRKQAEYCYSPTAEEAVTDYVRQELTSYKQLPVNLYQIQTKFRDEIRPRFGVMRAREFVMKDA
YSFHLNDADLVREYENMRATYTRIFTRLGLEFRAVQADSGAIGGDASQEFHVIADSGEDVLAFSTGSDYAANIEAAIAAT
PGPRLTANETLQKVSTPTQKRCEDVAALLDIPLQRVVKSIAVMTDSGFFLALLRGDHTLNDIKLSKLPGLANFRLANEVE
IARHLGSEPGFLGPVCPGMPIRIIADCEVAVMADFVVGANEVGFHLVGVNWGRDLPEPEVVADIRNVIEGDRAVDGGKIC
IARGIEVGHVFQLGRKYAEAMKATVLDEYGKAVTMTMGCYGIGVSRIVAAAIEQNHDVAGIIWPAPIAPWQVAVCVINPK
KDPVITAAAEMLLAELQSADVDTVLDDRGLRPGVMFADMELIGIPHRIVVSERGLAAGTYEYRARRTAMVENLDKTTLLT
RIKA

Sequences:

>Translated_564_residues
MRLSEFHLHTTKEIPADAELVSHRLMLRAGMIRKLASGLYTWSPLGLRVLRKVEAIVRDEMNRAGAVEMLLPTIQPRELW
EESERWEKFGSQLLKIKDRKQAEYCYSPTAEEAVTDYVRQELTSYKQLPVNLYQIQTKFRDEIRPRFGVMRAREFVMKDA
YSFHLNDADLVREYENMRATYTRIFTRLGLEFRAVQADSGAIGGDASQEFHVIADSGEDVLAFSTGSDYAANIEAAIAAT
PGPRLTANETLQKVSTPTQKRCEDVAALLDIPLQRVVKSIAVMTDSGFFLALLRGDHTLNDIKLSKLPGLANFRLANEVE
IARHLGSEPGFLGPVCPGMPIRIIADCEVAVMADFVVGANEVGFHLVGVNWGRDLPEPEVVADIRNVIEGDRAVDGGKIC
IARGIEVGHVFQLGRKYAEAMKATVLDEYGKAVTMTMGCYGIGVSRIVAAAIEQNHDVAGIIWPAPIAPWQVAVCVINPK
KDPVITAAAEMLLAELQSADVDTVLDDRGLRPGVMFADMELIGIPHRIVVSERGLAAGTYEYRARRTAMVENLDKTTLLT
RIKA
>Mature_564_residues
MRLSEFHLHTTKEIPADAELVSHRLMLRAGMIRKLASGLYTWSPLGLRVLRKVEAIVRDEMNRAGAVEMLLPTIQPRELW
EESERWEKFGSQLLKIKDRKQAEYCYSPTAEEAVTDYVRQELTSYKQLPVNLYQIQTKFRDEIRPRFGVMRAREFVMKDA
YSFHLNDADLVREYENMRATYTRIFTRLGLEFRAVQADSGAIGGDASQEFHVIADSGEDVLAFSTGSDYAANIEAAIAAT
PGPRLTANETLQKVSTPTQKRCEDVAALLDIPLQRVVKSIAVMTDSGFFLALLRGDHTLNDIKLSKLPGLANFRLANEVE
IARHLGSEPGFLGPVCPGMPIRIIADCEVAVMADFVVGANEVGFHLVGVNWGRDLPEPEVVADIRNVIEGDRAVDGGKIC
IARGIEVGHVFQLGRKYAEAMKATVLDEYGKAVTMTMGCYGIGVSRIVAAAIEQNHDVAGIIWPAPIAPWQVAVCVINPK
KDPVITAAAEMLLAELQSADVDTVLDDRGLRPGVMFADMELIGIPHRIVVSERGLAAGTYEYRARRTAMVENLDKTTLLT
RIKA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=219, Percent_Identity=42.4657534246575, Blast_Score=190, Evalue=4e-48,
Organism=Escherichia coli, GI1786392, Length=555, Percent_Identity=55.1351351351351, Blast_Score=613, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI115532348, Length=247, Percent_Identity=35.2226720647773, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI193203271, Length=85, Percent_Identity=32.9411764705882, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320931, Length=202, Percent_Identity=44.0594059405941, Blast_Score=193, Evalue=6e-50,
Organism=Drosophila melanogaster, GI24656200, Length=229, Percent_Identity=34.9344978165939, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_XYLF2 (B2I7W6)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830405.1
- ProteinModelPortal:   B2I7W6
- SMR:   B2I7W6
- GeneID:   6202816
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1727
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 62452; Mature: 62452

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSEFHLHTTKEIPADAELVSHRLMLRAGMIRKLASGLYTWSPLGLRVLRKVEAIVRDE
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MNRAGAVEMLLPTIQPRELWEESERWEKFGSQLLKIKDRKQAEYCYSPTAEEAVTDYVRQ
HCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHH
ELTSYKQLPVNLYQIQTKFRDEIRPRFGVMRAREFVMKDAYSFHLNDADLVREYENMRAT
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
YTRIFTRLGLEFRAVQADSGAIGGDASQEFHVIADSGEDVLAFSTGSDYAANIEAAIAAT
HHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCEEEEEEC
PGPRLTANETLQKVSTPTQKRCEDVAALLDIPLQRVVKSIAVMTDSGFFLALLRGDHTLN
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
DIKLSKLPGLANFRLANEVEIARHLGSEPGFLGPVCPGMPIRIIADCEVAVMADFVVGAN
CEEHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCC
EVGFHLVGVNWGRDLPEPEVVADIRNVIEGDRAVDGGKICIARGIEVGHVFQLGRKYAEA
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
MKATVLDEYGKAVTMTMGCYGIGVSRIVAAAIEQNHDVAGIIWPAPIAPWQVAVCVINPK
HHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCC
KDPVITAAAEMLLAELQSADVDTVLDDRGLRPGVMFADMELIGIPHRIVVSERGLAAGTY
CCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEECCCEECCCCEEEEECCCCCCCCH
EYRARRTAMVENLDKTTLLTRIKA
HHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MRLSEFHLHTTKEIPADAELVSHRLMLRAGMIRKLASGLYTWSPLGLRVLRKVEAIVRDE
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MNRAGAVEMLLPTIQPRELWEESERWEKFGSQLLKIKDRKQAEYCYSPTAEEAVTDYVRQ
HCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHH
ELTSYKQLPVNLYQIQTKFRDEIRPRFGVMRAREFVMKDAYSFHLNDADLVREYENMRAT
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
YTRIFTRLGLEFRAVQADSGAIGGDASQEFHVIADSGEDVLAFSTGSDYAANIEAAIAAT
HHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCEEEEEEC
PGPRLTANETLQKVSTPTQKRCEDVAALLDIPLQRVVKSIAVMTDSGFFLALLRGDHTLN
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
DIKLSKLPGLANFRLANEVEIARHLGSEPGFLGPVCPGMPIRIIADCEVAVMADFVVGAN
CEEHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCC
EVGFHLVGVNWGRDLPEPEVVADIRNVIEGDRAVDGGKICIARGIEVGHVFQLGRKYAEA
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
MKATVLDEYGKAVTMTMGCYGIGVSRIVAAAIEQNHDVAGIIWPAPIAPWQVAVCVINPK
HHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEECCC
KDPVITAAAEMLLAELQSADVDTVLDDRGLRPGVMFADMELIGIPHRIVVSERGLAAGTY
CCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEECCCEECCCCEEEEECCCCCCCCH
EYRARRTAMVENLDKTTLLTRIKA
HHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA