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The map label for this gene is purA

Identifier: 182682237

GI number: 182682237

Start: 1900465

End: 1901757

Strand: Reverse

Name: purA

Synonym: XfasM23_1719

Alternate gene names: 182682237

Gene position: 1901757-1900465 (Counterclockwise)

Preceding gene: 182682238

Following gene: 182682235

Centisome position: 75.0

GC content: 51.97

Gene sequence:

>1293_bases
ATGGGTCAGTCAGTTGTCGTGCTCGGTGCCCAGTGGGGCGATGAAGGAAAGGGCAAGATCGTCGACCTTCTCACCGAGGA
AATTGGTGCTGTTGTGCGTTTTCAGGGAGGTCACAATGCTGGCCACACTCTGGTTATCAATGCTAAGAAAACCGTTTTGC
ATCTTATTCCATCTGGAATTTTGCGGAATGGTGTGCTCTGCTTGATTGGCAACGGTGTAGTTATCTCGCCAGCCGCATTG
CGAAAGGAGATTGAGGAGCTGGAAGATACCGGTTTGGAAATACGTTCCCGTTTGAAGATTTCGCCAGCTGCGCCGTTGAT
CATGGAATATCACATTGCACTGGATCAAGCACGCGAGAAAGCGGCTGGCGGTAGGGCTATTGGTACGACCGGTCGAGGGA
TTGGTCCGGCGTACGAGGACAAGGTCGGGCGTCGTGGTATTCGTGTCGCTGACCTTCATTACCCAGACCAATTGGCGGAA
AAGCTACGTGCTGCGTTGGATTACCACAATTTTGTGTTGACTCGATATTTTGGTGTGGATGGGATGGATTTCCAGCGGAT
CTATGACGAGATGTTAGTGTTCGCTGAATACGTCGAGCCGATGAAATCTGATGTTGCGGGTATCCTCCATGACCTGCGCA
AGCAAGGCAAGCGAGTGCTCTTTGAGGGTGCCCAAGGGACATTGCTGGATATCGATCACGGCACCTATCCGTACGTGACT
AGCTCCAGCACGACGGTTGGTGGTGCTCTCAGTGGTGCTGGTGTTGGTGTCCAGGATATCGACTATGTGTTGGGCATTGC
TAAGGCTTATGCAACTCGTGTTGGAGGTGGTCCATTTCCAACCGAGTTGGATGATAAAATTGGTCAGGGTATCCGAGATC
GTGGTGTTGAGTACGGTGCTTCCACTGGTCGTCCACGCCGCTGTGGTTGGATGGACATTGTTGCGCTCAAGCGTGCTGTT
GCCATCAATGGGATTACCGGTCTGTGTATTACCAAATTGGATGTGCTGGACGGAATGGATAAGCTTAAGATCTGTATCGC
TTATGAGTACCACGATAAACGAAGTGAGTACGCTCCGTTGGATGCGCAAGGTTGGGAAGAATGCACCCCAGTGTACTTGG
AATTTCCAGGCTGGAATGAGAGCACGCATGGCATTACTTCGTGGGAGAAACTCCCTCCAGCAGCCCGCGCTTATCTGTGT
GCGCTGGAAGAGCTGGCTGGCTGTCCGATTGGTATCGTGAGCACTGGCCCAGACCGAGAGCACACGATCATGTTGCATGA
TCCTTTTGCTTGA

Upstream 100 bases:

>100_bases
GTGCTGTGATGGTGTCTTGAATAAAGGTGCTTAACAAATACTTGTATGTTCTATTCCGTGGCTCCTGTCGGTGCCAGAAA
AATCTGAGGAGTTACCCATT

Downstream 100 bases:

>100_bases
TTTCTTAGTGATTCAAGGTCGGAGGATTTATTGGGAATAATGGATGATGTGCTTGTCGTTACACATTTCCACATGTGGCC
ATTTGATTTTTCCGAACCTA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINAKKTVLHLIPSGILRNGVLCLIGNGVVISPAAL
RKEIEELEDTGLEIRSRLKISPAAPLIMEYHIALDQAREKAAGGRAIGTTGRGIGPAYEDKVGRRGIRVADLHYPDQLAE
KLRAALDYHNFVLTRYFGVDGMDFQRIYDEMLVFAEYVEPMKSDVAGILHDLRKQGKRVLFEGAQGTLLDIDHGTYPYVT
SSSTTVGGALSGAGVGVQDIDYVLGIAKAYATRVGGGPFPTELDDKIGQGIRDRGVEYGASTGRPRRCGWMDIVALKRAV
AINGITGLCITKLDVLDGMDKLKICIAYEYHDKRSEYAPLDAQGWEECTPVYLEFPGWNESTHGITSWEKLPPAARAYLC
ALEELAGCPIGIVSTGPDREHTIMLHDPFA

Sequences:

>Translated_430_residues
MGQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINAKKTVLHLIPSGILRNGVLCLIGNGVVISPAAL
RKEIEELEDTGLEIRSRLKISPAAPLIMEYHIALDQAREKAAGGRAIGTTGRGIGPAYEDKVGRRGIRVADLHYPDQLAE
KLRAALDYHNFVLTRYFGVDGMDFQRIYDEMLVFAEYVEPMKSDVAGILHDLRKQGKRVLFEGAQGTLLDIDHGTYPYVT
SSSTTVGGALSGAGVGVQDIDYVLGIAKAYATRVGGGPFPTELDDKIGQGIRDRGVEYGASTGRPRRCGWMDIVALKRAV
AINGITGLCITKLDVLDGMDKLKICIAYEYHDKRSEYAPLDAQGWEECTPVYLEFPGWNESTHGITSWEKLPPAARAYLC
ALEELAGCPIGIVSTGPDREHTIMLHDPFA
>Mature_429_residues
GQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINAKKTVLHLIPSGILRNGVLCLIGNGVVISPAALR
KEIEELEDTGLEIRSRLKISPAAPLIMEYHIALDQAREKAAGGRAIGTTGRGIGPAYEDKVGRRGIRVADLHYPDQLAEK
LRAALDYHNFVLTRYFGVDGMDFQRIYDEMLVFAEYVEPMKSDVAGILHDLRKQGKRVLFEGAQGTLLDIDHGTYPYVTS
SSTTVGGALSGAGVGVQDIDYVLGIAKAYATRVGGGPFPTELDDKIGQGIRDRGVEYGASTGRPRRCGWMDIVALKRAVA
INGITGLCITKLDVLDGMDKLKICIAYEYHDKRSEYAPLDAQGWEECTPVYLEFPGWNESTHGITSWEKLPPAARAYLCA
LEELAGCPIGIVSTGPDREHTIMLHDPFA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=41.92037470726, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=41.6279069767442, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=40.0503778337531, Blast_Score=285, Evalue=6e-77,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=59.5348837209302, Blast_Score=534, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI25146553, Length=431, Percent_Identity=42.4593967517401, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=431, Percent_Identity=42.4593967517401, Blast_Score=328, Evalue=3e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=43.1235431235431, Blast_Score=331, Evalue=1e-91,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=41.7452830188679, Blast_Score=324, Evalue=8e-89,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_XYLF2 (B2I7V8)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830397.1
- ProteinModelPortal:   B2I7V8
- SMR:   B2I7V8
- GeneID:   6202889
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1719
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46641; Mature: 46510

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINAKKTVLHLIPSGI
CCCCEEEEEECCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCEEEEECHHHHHHHHHHHH
LRNGVLCLIGNGVVISPAALRKEIEELEDTGLEIRSRLKISPAAPLIMEYHIALDQAREK
HHCCEEEEECCCEEECHHHHHHHHHHHHHCCHHHHHHEECCCCCCHHHHHHHHHHHHHHH
AAGGRAIGTTGRGIGPAYEDKVGRRGIRVADLHYPDQLAEKLRAALDYHNFVLTRYFGVD
HCCCCEECCCCCCCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEHEECCC
GMDFQRIYDEMLVFAEYVEPMKSDVAGILHDLRKQGKRVLFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEE
SSSTTVGGALSGAGVGVQDIDYVLGIAKAYATRVGGGPFPTELDDKIGQGIRDRGVEYGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHCCCCCCC
STGRPRRCGWMDIVALKRAVAINGITGLCITKLDVLDGMDKLKICIAYEYHDKRSEYAPL
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCC
DAQGWEECTPVYLEFPGWNESTHGITSWEKLPPAARAYLCALEELAGCPIGIVSTGPDRE
CCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
HTIMLHDPFA
CEEEEECCCC
>Mature Secondary Structure 
GQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINAKKTVLHLIPSGI
CCCEEEEEECCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCEEEEECHHHHHHHHHHHH
LRNGVLCLIGNGVVISPAALRKEIEELEDTGLEIRSRLKISPAAPLIMEYHIALDQAREK
HHCCEEEEECCCEEECHHHHHHHHHHHHHCCHHHHHHEECCCCCCHHHHHHHHHHHHHHH
AAGGRAIGTTGRGIGPAYEDKVGRRGIRVADLHYPDQLAEKLRAALDYHNFVLTRYFGVD
HCCCCEECCCCCCCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEHEECCC
GMDFQRIYDEMLVFAEYVEPMKSDVAGILHDLRKQGKRVLFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCEEE
SSSTTVGGALSGAGVGVQDIDYVLGIAKAYATRVGGGPFPTELDDKIGQGIRDRGVEYGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHCCCCCCC
STGRPRRCGWMDIVALKRAVAINGITGLCITKLDVLDGMDKLKICIAYEYHDKRSEYAPL
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCC
DAQGWEECTPVYLEFPGWNESTHGITSWEKLPPAARAYLCALEELAGCPIGIVSTGPDRE
CCCCCCCCCCEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
HTIMLHDPFA
CEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA