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The map label for this gene is rlmN [H]

Identifier: 182682234

GI number: 182682234

Start: 1895752

End: 1896957

Strand: Reverse

Name: rlmN [H]

Synonym: XfasM23_1716

Alternate gene names: 182682234

Gene position: 1896957-1895752 (Counterclockwise)

Preceding gene: 182682235

Following gene: 182682233

Centisome position: 74.81

GC content: 49.92

Gene sequence:

>1206_bases
GTGATTCCCAGTGTGACAACAACGACAACTAGTGGTGAGCCCATCGCTAGCGATGTTGCTCGTAAACAGAATCTGCTGGA
ACTTGACCGTGAGGGGTTGGAGCGCTTTTTCGAAGATGTTCTTGGTGAGAAACGCTATCGTGCCCATCAGGTCATGAAAT
GGATCCACCACCGTTATGTCGCTGATTTCGAGCAAATGACCGATGTTGGCAAAGCGTTGCGTACTAGGTTGCAGGCTTGC
GCTGAGGTCAGGGTCCCCCGTGTTGTCTTCGACAAGCACTCAGCCGATGGCACACACAAGTGGTTGCTGGCGATGGGCAC
TGACAGAAAAAATGCGATTGAGACTGTTTATATCCCGGACAAAGGGCGTGGTACGTTGTGTGTGTCTTCGCAAATAGGGT
GTGGATTGAACTGTACGTTTTGTTCGACAGCCACCCAAGGTTTTAACCGCAATCTGACCACGGCCGAGATTATCGGCCAG
GTTTGGGTTGCTGCCCGTCACCTTGGTAATGTGCCGCACCAGCAGCGTCGTCTTACGAATGTGGTAATGATGGGTATGGG
TGAGCCCTTAATGAATTTCGATAACGTTGTGCGTGCGATGAGCGTGATGCGTGATGATTTGGGCTACGGGTTGTCCAACA
AGCGTGTGACTTTATCGACTTCCGGTTTAGTTCCGATGATTGATCGCCTCTCGACTGAGAGTGATGTGTCTTTGGCTGTA
TCACTGCATGCGCCGAATGACAAACTGCGCGAACAACTTGTTCCGTTGAATAAGAAATATCCAATTGTTGAGCTGATGGC
ATCCTGCGAGCGTTATTTGTCTGTTAATCGTAAACGTGATTCTGTCACTTTTGAATACACTTTGATGAAGGGTGTTAATG
ACAAGCAAGAACATGCTCATGAGTTGGCCAAACTAATGCGGCAATTTGACTGTGCGATGCAGGTGAAGGGTGCAGCGAAG
GTTAACCTGATTCCATTCAACCCGTTTCCGGGCACCTGTTATGAACGTTCTACGGAAGTTGATATCCGTGCTTTCCAGAA
GATCCTGTTGGACGCACAAATTTTGGCCATGGTGCGTCGTACTCGTGGAGACGACATTGATGCAGCGTGCGGGCAATTGA
AAGGGCAAGTCGTGGATCGTACTCGCCGTCAAGCTGAGTTCCGCCGCACTATTGAAGATCGGGTTGGTCGAGATGTTGCA
GCGTGA

Upstream 100 bases:

>100_bases
GGTTCGGATTCGGTCGAGAATGCTGCGATTGAAGTGGCTTACTTTTTCGCTGCTACCGAAATTATTTTGCGATGAGATTG
TAGGCATGAATGGGTTTGCG

Downstream 100 bases:

>100_bases
TATATCCCTGGTTTTAGCGTTTTCTTCTTTACTGATTTTATCTGGCTGCTTAGTCATGTCTGATAAGAGGGAGACACGTA
TTAAGTCAGTTCAGAATCTC

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MIPSVTTTTTSGEPIASDVARKQNLLELDREGLERFFEDVLGEKRYRAHQVMKWIHHRYVADFEQMTDVGKALRTRLQAC
AEVRVPRVVFDKHSADGTHKWLLAMGTDRKNAIETVYIPDKGRGTLCVSSQIGCGLNCTFCSTATQGFNRNLTTAEIIGQ
VWVAARHLGNVPHQQRRLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLSNKRVTLSTSGLVPMIDRLSTESDVSLAV
SLHAPNDKLREQLVPLNKKYPIVELMASCERYLSVNRKRDSVTFEYTLMKGVNDKQEHAHELAKLMRQFDCAMQVKGAAK
VNLIPFNPFPGTCYERSTEVDIRAFQKILLDAQILAMVRRTRGDDIDAACGQLKGQVVDRTRRQAEFRRTIEDRVGRDVA
A

Sequences:

>Translated_401_residues
MIPSVTTTTTSGEPIASDVARKQNLLELDREGLERFFEDVLGEKRYRAHQVMKWIHHRYVADFEQMTDVGKALRTRLQAC
AEVRVPRVVFDKHSADGTHKWLLAMGTDRKNAIETVYIPDKGRGTLCVSSQIGCGLNCTFCSTATQGFNRNLTTAEIIGQ
VWVAARHLGNVPHQQRRLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLSNKRVTLSTSGLVPMIDRLSTESDVSLAV
SLHAPNDKLREQLVPLNKKYPIVELMASCERYLSVNRKRDSVTFEYTLMKGVNDKQEHAHELAKLMRQFDCAMQVKGAAK
VNLIPFNPFPGTCYERSTEVDIRAFQKILLDAQILAMVRRTRGDDIDAACGQLKGQVVDRTRRQAEFRRTIEDRVGRDVA
A
>Mature_401_residues
MIPSVTTTTTSGEPIASDVARKQNLLELDREGLERFFEDVLGEKRYRAHQVMKWIHHRYVADFEQMTDVGKALRTRLQAC
AEVRVPRVVFDKHSADGTHKWLLAMGTDRKNAIETVYIPDKGRGTLCVSSQIGCGLNCTFCSTATQGFNRNLTTAEIIGQ
VWVAARHLGNVPHQQRRLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLSNKRVTLSTSGLVPMIDRLSTESDVSLAV
SLHAPNDKLREQLVPLNKKYPIVELMASCERYLSVNRKRDSVTFEYTLMKGVNDKQEHAHELAKLMRQFDCAMQVKGAAK
VNLIPFNPFPGTCYERSTEVDIRAFQKILLDAQILAMVRRTRGDDIDAACGQLKGQVVDRTRRQAEFRRTIEDRVGRDVA
A

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=364, Percent_Identity=54.9450549450549, Blast_Score=409, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 45218; Mature: 45218

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPSVTTTTTSGEPIASDVARKQNLLELDREGLERFFEDVLGEKRYRAHQVMKWIHHRYV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
ADFEQMTDVGKALRTRLQACAEVRVPRVVFDKHSADGTHKWLLAMGTDRKNAIETVYIPD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCEEEEEEECCCCCCCEEEEEECC
KGRGTLCVSSQIGCGLNCTFCSTATQGFNRNLTTAEIIGQVWVAARHLGNVPHQQRRLTN
CCCCEEEEECCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLSNKRVTLSTSGLVPMIDRLSTESDVSLAV
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCCCEEEE
SLHAPNDKLREQLVPLNKKYPIVELMASCERYLSVNRKRDSVTFEYTLMKGVNDKQEHAH
EEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHH
ELAKLMRQFDCAMQVKGAAKVNLIPFNPFPGTCYERSTEVDIRAFQKILLDAQILAMVRR
HHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TRGDDIDAACGQLKGQVVDRTRRQAEFRRTIEDRVGRDVAA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIPSVTTTTTSGEPIASDVARKQNLLELDREGLERFFEDVLGEKRYRAHQVMKWIHHRYV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
ADFEQMTDVGKALRTRLQACAEVRVPRVVFDKHSADGTHKWLLAMGTDRKNAIETVYIPD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCEEEEEEECCCCCCCEEEEEECC
KGRGTLCVSSQIGCGLNCTFCSTATQGFNRNLTTAEIIGQVWVAARHLGNVPHQQRRLTN
CCCCEEEEECCCCCCCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLSNKRVTLSTSGLVPMIDRLSTESDVSLAV
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCCCEEEE
SLHAPNDKLREQLVPLNKKYPIVELMASCERYLSVNRKRDSVTFEYTLMKGVNDKQEHAH
EEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHH
ELAKLMRQFDCAMQVKGAAKVNLIPFNPFPGTCYERSTEVDIRAFQKILLDAQILAMVRR
HHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TRGDDIDAACGQLKGQVVDRTRRQAEFRRTIEDRVGRDVAA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA