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The map label for this gene is gph [H]

Identifier: 182682095

GI number: 182682095

Start: 1745811

End: 1746500

Strand: Reverse

Name: gph [H]

Synonym: XfasM23_1573

Alternate gene names: 182682095

Gene position: 1746500-1745811 (Counterclockwise)

Preceding gene: 182682096

Following gene: 182682094

Centisome position: 68.88

GC content: 56.96

Gene sequence:

>690_bases
ATGCCACTGAATGAAGTGTCCGGCAACGCCTTCCCCCGTACCGTGTTGTTTGATTTGGATGGCACATTGCTTGATAGCGC
CCCCGACATGCTTGCCACTGCCAACGCCATGTTGGCTGCACGTGGTCGTGCCCCAATCACCTTGATGCAACTACGTCCGG
TGATTTCCAGAGGCACATTTAGGATTATTGCTGTGGCTTTCCCGGAACTGGATGCCGCAGCCATCCAGGGACTGATACCG
GAGTTTCTCCAGCGTTATGAAGCACTGATTGGTAGCGTCTCCAAGCCATTCGATGGCGTTGAAATGATGCTTCATGCTCT
GGAGTGCGCTGGGACGGTGTGGGGCATTGTTACCAACAAACCGGAGTTTTTAGCGCGGCTGATCTTACCGTTGCTGGGAT
GGACATCCCGTTGCGCCGTGCTGATCGGCGGAGATACCCTCGCCGAGCGTAAACCGCATCCCCTACCACTGTTGACGGCC
GCTGAGCGTATTGGCGTCATGCCTACAGACTGTGTCTACGTGGGGGACGATGTCAGAGACATCCAGGCGGCACGTGCGGC
GGGTATGCCCTCAATGGTTGCACTGTGGGGATATCGCTCCCATGAAGACAACCCCATGACCTGGCAGGCCGATACCCTGG
TTGAACAACCCCACCTGTTGAGTCGGCCCGATGTTTGGCCCAGCACCTGA

Upstream 100 bases:

>100_bases
GTCAGCGGCATGCGGTACGAGCCGTGGCGCAATCGTGCCCGGCTTACCGCTCGCACTGACATCAACTATCTTGCTTGCGC
CATCTCCCCTGAGGCTTCGC

Downstream 100 bases:

>100_bases
ACATCGCCTTGTTGTATTGATCGTGTACTGACGGTGACTGGCTCCGTTGCTCTGGATAGCTTCCTGGACAAGTGGCTGGC
CCGCTGGCCGCAATGGTCAG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPLNEVSGNAFPRTVLFDLDGTLLDSAPDMLATANAMLAARGRAPITLMQLRPVISRGTFRIIAVAFPELDAAAIQGLIP
EFLQRYEALIGSVSKPFDGVEMMLHALECAGTVWGIVTNKPEFLARLILPLLGWTSRCAVLIGGDTLAERKPHPLPLLTA
AERIGVMPTDCVYVGDDVRDIQAARAAGMPSMVALWGYRSHEDNPMTWQADTLVEQPHLLSRPDVWPST

Sequences:

>Translated_229_residues
MPLNEVSGNAFPRTVLFDLDGTLLDSAPDMLATANAMLAARGRAPITLMQLRPVISRGTFRIIAVAFPELDAAAIQGLIP
EFLQRYEALIGSVSKPFDGVEMMLHALECAGTVWGIVTNKPEFLARLILPLLGWTSRCAVLIGGDTLAERKPHPLPLLTA
AERIGVMPTDCVYVGDDVRDIQAARAAGMPSMVALWGYRSHEDNPMTWQADTLVEQPHLLSRPDVWPST
>Mature_228_residues
PLNEVSGNAFPRTVLFDLDGTLLDSAPDMLATANAMLAARGRAPITLMQLRPVISRGTFRIIAVAFPELDAAAIQGLIPE
FLQRYEALIGSVSKPFDGVEMMLHALECAGTVWGIVTNKPEFLARLILPLLGWTSRCAVLIGGDTLAERKPHPLPLLTAA
ERIGVMPTDCVYVGDDVRDIQAARAAGMPSMVALWGYRSHEDNPMTWQADTLVEQPHLLSRPDVWPST

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=212, Percent_Identity=34.9056603773585, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24926; Mature: 24795

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLNEVSGNAFPRTVLFDLDGTLLDSAPDMLATANAMLAARGRAPITLMQLRPVISRGTF
CCCCCCCCCCCCEEEEEECCCHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCE
RIIAVAFPELDAAAIQGLIPEFLQRYEALIGSVSKPFDGVEMMLHALECAGTVWGIVTNK
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCC
PEFLARLILPLLGWTSRCAVLIGGDTLAERKPHPLPLLTAAERIGVMPTDCVYVGDDVRD
HHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCCCHHHHHHHHHCCCCCCEEEECCCHHH
IQAARAAGMPSMVALWGYRSHEDNPMTWQADTLVEQPHLLSRPDVWPST
HHHHHHCCCCHHHHHHCCCCCCCCCCEEEHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLNEVSGNAFPRTVLFDLDGTLLDSAPDMLATANAMLAARGRAPITLMQLRPVISRGTF
CCCCCCCCCCCEEEEEECCCHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCE
RIIAVAFPELDAAAIQGLIPEFLQRYEALIGSVSKPFDGVEMMLHALECAGTVWGIVTNK
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEECCC
PEFLARLILPLLGWTSRCAVLIGGDTLAERKPHPLPLLTAAERIGVMPTDCVYVGDDVRD
HHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCCCHHHHHHHHHCCCCCCEEEECCCHHH
IQAARAAGMPSMVALWGYRSHEDNPMTWQADTLVEQPHLLSRPDVWPST
HHHHHHCCCCHHHHHHCCCCCCCCCCEEEHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]