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The map label for this gene is yafV [C]

Identifier: 182682067

GI number: 182682067

Start: 1716297

End: 1717184

Strand: Direct

Name: yafV [C]

Synonym: XfasM23_1545

Alternate gene names: 182682067

Gene position: 1716297-1717184 (Clockwise)

Preceding gene: 182682066

Following gene: 182682068

Centisome position: 67.69

GC content: 52.93

Gene sequence:

>888_bases
ATGACAACTCATCTTCTCCCCGTAGCGCTGATCCAAGAGCGCAACCATGGCACTGCGGAAGCCAATTTGAGCATCATCGA
AGCACGTGTCGCCGAAGCTGCCGCACAGGGCGCTCAACTGGTGCTACTGCATGAATTACACAACAACGCATACTTCTGCC
AACATGAATCGGTCAACGAATTCGACTTAGCAGAGCCAATTCCAGGTCCAAGTACCGAACGTCTAAGCGCACTGGCGAAA
CAACATCGTGTGGTTATTATTGGCTCACTGTTTGAGAAGCGTGCCGCAGGCCTCTATCACAATACTGCAGTGGTACTAGA
AAAGGACGGCAGGCTAGTTGGCAAATACCGTAAGATGCACATTCCAGATGACCCTGGTTTCTATGAGAAGTTCTACTTCA
CCCCAGGTGATATCGGCTTCAAACCAATCGATACATCTGTCGGCCGTCTCGGTGTTCTAGTGTGCTGGGATCAATGGTAC
CCAGAAGCAGCACGACTAATGGCGCTTGCAGGTGCCGAACTACTGCTATACCCAACTGCAATCGGCTGGGATCCGAATGA
TGAACACGACGAGCAAACCCTGCAGCGCGACGCTTGGTTACTGAGCCACCGTGGCCACGCCATTGCAAATAGCCTACCCG
TACTCAGTTGCAACCGTACCGGCCACGAACCATCGCCACTTTGCACCTCAGGCATTCATTTTTGGGGAAACAGCCACGTC
CTCGGCCCACAAGGCGAGTTCCTCGCTGAAGCCAACAGCAACGGACCAGAAATTTTGACATGCGAAATCAACCTACAACG
CAGCGAGCACGTACGGCGGATCTGGCCATTTCTGCGCGACCGTCGCATCGACGCCTACGGCGACTTACTCAAACGTTACA
TTGACTAG

Upstream 100 bases:

>100_bases
CTTGCCACATAAACACATGTATTGGATAACACTGCGCTGAGGCCGCAACCCCTCTCAAGATTACACACGAGCCCCCCATT
GCGCATTCTTCATATATGTC

Downstream 100 bases:

>100_bases
ACGGTAAACACCTCAGCCTTACAGGCACGCATGAATGAGAAGCCAATGATCACACCTGCCCACCACCAGACAGCACATCC
GTCTAGTGTGGCTCACCTTC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTTHLLPVALIQERNHGTAEANLSIIEARVAEAAAQGAQLVLLHELHNNAYFCQHESVNEFDLAEPIPGPSTERLSALAK
QHRVVIIGSLFEKRAAGLYHNTAVVLEKDGRLVGKYRKMHIPDDPGFYEKFYFTPGDIGFKPIDTSVGRLGVLVCWDQWY
PEAARLMALAGAELLLYPTAIGWDPNDEHDEQTLQRDAWLLSHRGHAIANSLPVLSCNRTGHEPSPLCTSGIHFWGNSHV
LGPQGEFLAEANSNGPEILTCEINLQRSEHVRRIWPFLRDRRIDAYGDLLKRYID

Sequences:

>Translated_295_residues
MTTHLLPVALIQERNHGTAEANLSIIEARVAEAAAQGAQLVLLHELHNNAYFCQHESVNEFDLAEPIPGPSTERLSALAK
QHRVVIIGSLFEKRAAGLYHNTAVVLEKDGRLVGKYRKMHIPDDPGFYEKFYFTPGDIGFKPIDTSVGRLGVLVCWDQWY
PEAARLMALAGAELLLYPTAIGWDPNDEHDEQTLQRDAWLLSHRGHAIANSLPVLSCNRTGHEPSPLCTSGIHFWGNSHV
LGPQGEFLAEANSNGPEILTCEINLQRSEHVRRIWPFLRDRRIDAYGDLLKRYID
>Mature_294_residues
TTHLLPVALIQERNHGTAEANLSIIEARVAEAAAQGAQLVLLHELHNNAYFCQHESVNEFDLAEPIPGPSTERLSALAKQ
HRVVIIGSLFEKRAAGLYHNTAVVLEKDGRLVGKYRKMHIPDDPGFYEKFYFTPGDIGFKPIDTSVGRLGVLVCWDQWYP
EAARLMALAGAELLLYPTAIGWDPNDEHDEQTLQRDAWLLSHRGHAIANSLPVLSCNRTGHEPSPLCTSGIHFWGNSHVL
GPQGEFLAEANSNGPEILTCEINLQRSEHVRRIWPFLRDRRIDAYGDLLKRYID

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=297, Percent_Identity=31.6498316498317, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI7706509, Length=284, Percent_Identity=28.169014084507, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI297632350, Length=294, Percent_Identity=28.9115646258503, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI5031947, Length=294, Percent_Identity=28.9115646258503, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI297632348, Length=294, Percent_Identity=28.9115646258503, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI297632346, Length=195, Percent_Identity=29.7435897435897, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17533173, Length=311, Percent_Identity=29.5819935691318, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17556280, Length=288, Percent_Identity=26.3888888888889, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=265, Percent_Identity=30.9433962264151, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6322335, Length=318, Percent_Identity=23.2704402515723, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21358471, Length=288, Percent_Identity=30.5555555555556, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355835, Length=297, Percent_Identity=28.6195286195286, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17933642, Length=286, Percent_Identity=26.9230769230769, Blast_Score=98, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33067; Mature: 32936

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHLLPVALIQERNHGTAEANLSIIEARVAEAAAQGAQLVLLHELHNNAYFCQHESVNE
CCCCCEEHHEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCC
FDLAEPIPGPSTERLSALAKQHRVVIIGSLFEKRAAGLYHNTAVVLEKDGRLVGKYRKMH
CCCCCCCCCCCHHHHHHHHHCCCEEEEECHHHHHHCCCEECEEEEEECCCCEEEEEEEEC
IPDDPGFYEKFYFTPGDIGFKPIDTSVGRLGVLVCWDQWYPEAARLMALAGAELLLYPTA
CCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEE
IGWDPNDEHDEQTLQRDAWLLSHRGHAIANSLPVLSCNRTGHEPSPLCTSGIHFWGNSHV
ECCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCEEECCCCCCCCCCCCCCCEEECCCCEE
LGPQGEFLAEANSNGPEILTCEINLQRSEHVRRIWPFLRDRRIDAYGDLLKRYID
ECCCCCEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TTHLLPVALIQERNHGTAEANLSIIEARVAEAAAQGAQLVLLHELHNNAYFCQHESVNE
CCCCEEHHEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCC
FDLAEPIPGPSTERLSALAKQHRVVIIGSLFEKRAAGLYHNTAVVLEKDGRLVGKYRKMH
CCCCCCCCCCCHHHHHHHHHCCCEEEEECHHHHHHCCCEECEEEEEECCCCEEEEEEEEC
IPDDPGFYEKFYFTPGDIGFKPIDTSVGRLGVLVCWDQWYPEAARLMALAGAELLLYPTA
CCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEE
IGWDPNDEHDEQTLQRDAWLLSHRGHAIANSLPVLSCNRTGHEPSPLCTSGIHFWGNSHV
ECCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCEEECCCCCCCCCCCCCCCEEECCCCEE
LGPQGEFLAEANSNGPEILTCEINLQRSEHVRRIWPFLRDRRIDAYGDLLKRYID
ECCCCCEEEECCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]