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The map label for this gene is rumA

Identifier: 182681995

GI number: 182681995

Start: 1621457

End: 1622863

Strand: Reverse

Name: rumA

Synonym: XfasM23_1470

Alternate gene names: 182681995

Gene position: 1622863-1621457 (Counterclockwise)

Preceding gene: 182681997

Following gene: 182681994

Centisome position: 64.0

GC content: 52.1

Gene sequence:

>1407_bases
GTGGATGGTGGGCTGGGTTCAGTATGGAACGCAATCTGCGACAATGTTTCGAATCTTATTTGCAGTGTTTCTTTTGTGGC
TCGCCAAAATCGTTTCGATCGTACGTCATTTCAAACGCAGATTATTGATCTCAGTCATGATGGGCGTGGGGTTGCACGAC
CTCATGGCGAGGGAGGTAAGGTGACTTTCGTCACTGGCGCGCTTCCAGGTGAGGTGGTGATTGTTGAGCCAGTTGCGCGT
AATCGTCATTTTGATGAGGCACGTGTCGTTGAGGTGTTGCAGGCATCGCCGCAACGGGTGATACCGCGTTGCTCGCATTT
TGGTGTCTGTTCTGGTTGTGTATTGCAGCATTTGGCTGAAGATGCGCAGGTTGTGTCGAAGCAACGGGTGTTGTTGGAAA
GTTTGGAACGTATTGGTTGTGTCAGTCCGGAGCGTGTGTTGCCGGCGTTGGCCGCGGAGAGCTGGGGGTATCGGCGTAAG
GGGCGTTTTTCTGTACGTTGGGTTGAGAAAAAGGGGAGGACGCTGGTTGGTTTCCGTGAGCATGATCCGCGTTTTGTTGC
CGATGTGTCGGTCTGTCATACAGTGGTTCCGCAGGTCGGTGAAAAAATTGCGCTTTTGGCAACGTTGCTTGACAGTTTGG
ACGGTAGGTGTGACGTACCGCAGATTGAGTTTATTGCCGGTGATGCTGTTGTGGCATTAACGGTGCGTCATTTGCAGCCG
TTGAGTGAGGCGGATCGTTTGGCGCTGATTGAGTTTGGCAAGGAGCATGGTATTGCAATTTTTTTGCAGTCGGGTGGGGT
GGAGAGTGTGCGTTTGTTGTGGCCTGATGAGGTGTTGTTGGCATTTCGCTTGAAGCCTTGGGATGTGGAGTTTGTGTTCC
GTCCGCTGGATTTCATTCAGATCAATGGTGGGCTGAATGAGAAGATGATTGCGCATGCTTTGGATCTGCTCGGTGCTGGA
TTTGGGGAGCGTGTGCTCGATCTGTTTTGTGGTTTGGGTAACTTCACGTTGCCGTTGGCGCGTACTGTGGGTGAGGTGGT
TGGAGTGGAGGGTGATATTGGGCTGGTGGAGCGGGCCAGGGAGAATGCACGGCGTAATGGTTTGGGGAATGCTGAATTTT
TTGTTGCTGATTTGACCCGTGATCAACGCGATGCGCCGTGGATGCGTCAGGGCTTTGATAAGTTGCTGTTGGATCCGCCG
CGTTCCGGAGCGATTGAGGTACTCAAGCAGTTGCCGTTAAAGATGTTCGAACGCATCGTTTATGTCAGTTGTCATCCTGG
ATCTCTGGCGCGTGATGCTGATTTCCTTGTCAATGAACAGGGCTTTGTGTTGCGTGCGGTTGGGGCGATGGACATGTTTC
CGCATACTGCGCATGTGGAGAGTATTGCTGTATTTGATCGTTGCTGA

Upstream 100 bases:

>100_bases
TCATTACTCACAATATGTATGTTCAAAACTCTCTTAGAATTCTGCTCTTGTTTATTTATTTGTACAACAGTTGTTCTTTA
TTTTTTGTTTGGATGGATAT

Downstream 100 bases:

>100_bases
GTGATCCGAAGAGTGTTGCACGGTCTGATTCGCTCTGACTTTTTCTGAATTTTATAAAATTATGGCTATCGAAATAGAAC
GTAAATTCCTGGTTGTTGGG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MDGGLGSVWNAICDNVSNLICSVSFVARQNRFDRTSFQTQIIDLSHDGRGVARPHGEGGKVTFVTGALPGEVVIVEPVAR
NRHFDEARVVEVLQASPQRVIPRCSHFGVCSGCVLQHLAEDAQVVSKQRVLLESLERIGCVSPERVLPALAAESWGYRRK
GRFSVRWVEKKGRTLVGFREHDPRFVADVSVCHTVVPQVGEKIALLATLLDSLDGRCDVPQIEFIAGDAVVALTVRHLQP
LSEADRLALIEFGKEHGIAIFLQSGGVESVRLLWPDEVLLAFRLKPWDVEFVFRPLDFIQINGGLNEKMIAHALDLLGAG
FGERVLDLFCGLGNFTLPLARTVGEVVGVEGDIGLVERARENARRNGLGNAEFFVADLTRDQRDAPWMRQGFDKLLLDPP
RSGAIEVLKQLPLKMFERIVYVSCHPGSLARDADFLVNEQGFVLRAVGAMDMFPHTAHVESIAVFDRC

Sequences:

>Translated_468_residues
MDGGLGSVWNAICDNVSNLICSVSFVARQNRFDRTSFQTQIIDLSHDGRGVARPHGEGGKVTFVTGALPGEVVIVEPVAR
NRHFDEARVVEVLQASPQRVIPRCSHFGVCSGCVLQHLAEDAQVVSKQRVLLESLERIGCVSPERVLPALAAESWGYRRK
GRFSVRWVEKKGRTLVGFREHDPRFVADVSVCHTVVPQVGEKIALLATLLDSLDGRCDVPQIEFIAGDAVVALTVRHLQP
LSEADRLALIEFGKEHGIAIFLQSGGVESVRLLWPDEVLLAFRLKPWDVEFVFRPLDFIQINGGLNEKMIAHALDLLGAG
FGERVLDLFCGLGNFTLPLARTVGEVVGVEGDIGLVERARENARRNGLGNAEFFVADLTRDQRDAPWMRQGFDKLLLDPP
RSGAIEVLKQLPLKMFERIVYVSCHPGSLARDADFLVNEQGFVLRAVGAMDMFPHTAHVESIAVFDRC
>Mature_468_residues
MDGGLGSVWNAICDNVSNLICSVSFVARQNRFDRTSFQTQIIDLSHDGRGVARPHGEGGKVTFVTGALPGEVVIVEPVAR
NRHFDEARVVEVLQASPQRVIPRCSHFGVCSGCVLQHLAEDAQVVSKQRVLLESLERIGCVSPERVLPALAAESWGYRRK
GRFSVRWVEKKGRTLVGFREHDPRFVADVSVCHTVVPQVGEKIALLATLLDSLDGRCDVPQIEFIAGDAVVALTVRHLQP
LSEADRLALIEFGKEHGIAIFLQSGGVESVRLLWPDEVLLAFRLKPWDVEFVFRPLDFIQINGGLNEKMIAHALDLLGAG
FGERVLDLFCGLGNFTLPLARTVGEVVGVEGDIGLVERARENARRNGLGNAEFFVADLTRDQRDAPWMRQGFDKLLLDPP
RSGAIEVLKQLPLKMFERIVYVSCHPGSLARDADFLVNEQGFVLRAVGAMDMFPHTAHVESIAVFDRC

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=192, Percent_Identity=34.8958333333333, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI51173878, Length=192, Percent_Identity=34.8958333333333, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789148, Length=447, Percent_Identity=38.255033557047, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI1790403, Length=185, Percent_Identity=29.1891891891892, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787083, Length=182, Percent_Identity=31.8681318681319, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24666579, Length=198, Percent_Identity=32.3232323232323, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 51653; Mature: 51653

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGGLGSVWNAICDNVSNLICSVSFVARQNRFDRTSFQTQIIDLSHDGRGVARPHGEGGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCE
VTFVTGALPGEVVIVEPVARNRHFDEARVVEVLQASPQRVIPRCSHFGVCSGCVLQHLAE
EEEEECCCCCCEEEECCHHCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHH
DAQVVSKQRVLLESLERIGCVSPERVLPALAAESWGYRRKGRFSVRWVEKKGRTLVGFRE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEC
HDPRFVADVSVCHTVVPQVGEKIALLATLLDSLDGRCDVPQIEFIAGDAVVALTVRHLQP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHEEHHHHHHCC
LSEADRLALIEFGKEHGIAIFLQSGGVESVRLLWPDEVLLAFRLKPWDVEFVFRPLDFIQ
CCCCCCEEEEHCCCCCCEEEEEECCCCCEEEEECCCCEEEEEECCCCCEEEEECCCEEEE
INGGLNEKMIAHALDLLGAGFGERVLDLFCGLGNFTLPLARTVGEVVGVEGDIGLVERAR
ECCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
ENARRNGLGNAEFFVADLTRDQRDAPWMRQGFDKLLLDPPRSGAIEVLKQLPLKMFERIV
HHHHHCCCCCCEEEEEECCCCCCCCCHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHEE
YVSCHPGSLARDADFLVNEQGFVLRAVGAMDMFPHTAHVESIAVFDRC
EEEECCCCCCCCCHHEECCCCCEEEEECHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MDGGLGSVWNAICDNVSNLICSVSFVARQNRFDRTSFQTQIIDLSHDGRGVARPHGEGGK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCE
VTFVTGALPGEVVIVEPVARNRHFDEARVVEVLQASPQRVIPRCSHFGVCSGCVLQHLAE
EEEEECCCCCCEEEECCHHCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHH
DAQVVSKQRVLLESLERIGCVSPERVLPALAAESWGYRRKGRFSVRWVEKKGRTLVGFRE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEEC
HDPRFVADVSVCHTVVPQVGEKIALLATLLDSLDGRCDVPQIEFIAGDAVVALTVRHLQP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHEEHHHHHHCC
LSEADRLALIEFGKEHGIAIFLQSGGVESVRLLWPDEVLLAFRLKPWDVEFVFRPLDFIQ
CCCCCCEEEEHCCCCCCEEEEEECCCCCEEEEECCCCEEEEEECCCCCEEEEECCCEEEE
INGGLNEKMIAHALDLLGAGFGERVLDLFCGLGNFTLPLARTVGEVVGVEGDIGLVERAR
ECCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
ENARRNGLGNAEFFVADLTRDQRDAPWMRQGFDKLLLDPPRSGAIEVLKQLPLKMFERIV
HHHHHCCCCCCEEEEEECCCCCCCCCHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHEE
YVSCHPGSLARDADFLVNEQGFVLRAVGAMDMFPHTAHVESIAVFDRC
EEEECCCCCCCCCHHEECCCCCEEEEECHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]