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The map label for this gene is lepA

Identifier: 182681904

GI number: 182681904

Start: 1519331

End: 1521139

Strand: Direct

Name: lepA

Synonym: XfasM23_1372

Alternate gene names: 182681904

Gene position: 1519331-1521139 (Clockwise)

Preceding gene: 182681903

Following gene: 182681905

Centisome position: 59.92

GC content: 51.58

Gene sequence:

>1809_bases
ATGTCATCCGATCCGATGAGGAACATTCGCAACTTTTCTATCATTGCCCATGTCGACCATGGCAAGTCCACCCTGGCCGA
CCGCATCATTCAGTTGTGCGGTGGCCTAGAGGCACGTGAGATGGAAGCGCAAGTGCTCGACTCCAACCCAATCGAACGGG
AACGTGGCATCACTATCAAAGCCCAGTCCGTATCGCTGCTGTATAAAGCACAGGACGGACAAAACTACCATCTGAACTTA
ATTGACACCCCCGGACACGTCGATTTCTCCTACGAAGTCAGCCGCTCATTAGCAGCCTGTGAAGGTGCATTGCTGGTCGT
GGATGCGTCTCAAGGTGTGGAAGCGCAATCCGTCGCCAACTGTTACACCGCAGTGGAACAAGGGCTAGAAGTCGTACCCA
TTCTGAACAAAATCGACCTACCCACCGCCGATACTGAACGGGCTAAGGCAGAAATCGAAACAGTGATTGGTATTGACGCC
AGCGAAGCAGTCGCCGTGAGTGCCAAGACCGGCCTTTACGTGGAGCAAGTGCTAGAAGCCATCGTACAGCGCATCCCAGC
ACCACAACCACGTGATACGGAGAAGTTGCAAGCTCTTATCATTGACTCCTGGTTCGACAACTACCTAGGTGTGGTTTCAC
TGGTACGTGTTATGCAGGGCGAGATCACACCTGGCAACAAACTGTTAGTGATGTCCACAGGACGCAGCCACCAAGTGGAC
GCAGTCGGCGTCTTCACACCTAAACGCAAGACACTGGCAAAGCTGACCGCCGGTGAAGTTGGTTGGGTCACCGCATCAAT
CAAAGATGTACACGGCGCGCCCGTCGGGGACACACTGACTCTTACCAGCGATCCGGCACCCAAACCACTGCCAGGTTTCC
AAGAGGTACAGCCACGCGTGTTCGCCGGCTTATTTCCGGTCGATGCTGAGGACTATCCCGACCTACGCGAAGCACTGGAA
AAACTGCGCCTGAACGATGCGGCACTACGCTTTGAACCTGAAAATTCAGAAGCAATGGGCTTCGGCTTCCGTTGCGGCTT
TCTAGGCATGCTGCACATGGAAATCGTACAAGAGCGTCTAGAACGTGAGTACGATCTGAACCTCATCACCACTGCACCGA
CAGTCATCTATGAAGTACTTAAAAACGACGGCACCCTCGTTGCAATGGACAACCCGGCAAAGATGCCTCCCATCAATCAA
ATTAACGAGATCCGTGAACCGATTATCCGTTCCAATATCTTGACCCCGCCAGATTATGTGGGTGCAGTAATCACACTGTG
CGAGGAGAAACGGGGCAGCCAAATCAGCATCACCTACTTAGGAAATCAGGTGCAAGTGGCCTACGAGCTGCCAATGGCAG
AGGTCGTGTTGGACTTCTTCGATAAACTCAAGTCAGTCACTCGTGGTTACGCCTCATTGGATTACCACTTCTTACGCTTC
CAGGAAGGACCGTTTGTTCGGGTGGACACACTGATCAATGGTGACCGGGTGGATGCACTGAGTGTCATCGTGCATCGTCA
TCAAGCCGAACGCCGTGGCCGTGAATTGTGCGAAAAAATGAAAGACTTAATACCGCGGCAGATGTTCGACGTCGCAATAC
AGGCCGCTATAGGATCGCAAATCATCTCACGCTCAACCGTCAAGGCGATGCGAAAAAATGTGCTGGCTAAATGCTACGGT
GGCGATATCAGTCGTAAGAAAAAACTGCTTGAGAAACAGAAAGAAGGCAAAAAACGGATGAAACAAATTGGTCGGGTGGA
AATCCCACAAGAAGCATTTTTAGCCGTACTACAGATAGACAATAAATAA

Upstream 100 bases:

>100_bases
GACCGAACTTCATCAATACTCAAGTATTCCTGAGTACGCTCACTCTGAACCATGCGATAATCCTGCGCTATCCCGACGAT
GGTTCCGGTGCCCCATCTCC

Downstream 100 bases:

>100_bases
ACTGGGACTGGTTCAAGGAACCAAGCAGTAATGACATGACTACTCCACGTCGCTTCTGCCCACTTACTTGCACCATTCCC
TATTCCCATCGTACTAAGGA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MSSDPMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLEAREMEAQVLDSNPIERERGITIKAQSVSLLYKAQDGQNYHLNL
IDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQSVANCYTAVEQGLEVVPILNKIDLPTADTERAKAEIETVIGIDA
SEAVAVSAKTGLYVEQVLEAIVQRIPAPQPRDTEKLQALIIDSWFDNYLGVVSLVRVMQGEITPGNKLLVMSTGRSHQVD
AVGVFTPKRKTLAKLTAGEVGWVTASIKDVHGAPVGDTLTLTSDPAPKPLPGFQEVQPRVFAGLFPVDAEDYPDLREALE
KLRLNDAALRFEPENSEAMGFGFRCGFLGMLHMEIVQERLEREYDLNLITTAPTVIYEVLKNDGTLVAMDNPAKMPPINQ
INEIREPIIRSNILTPPDYVGAVITLCEEKRGSQISITYLGNQVQVAYELPMAEVVLDFFDKLKSVTRGYASLDYHFLRF
QEGPFVRVDTLINGDRVDALSVIVHRHQAERRGRELCEKMKDLIPRQMFDVAIQAAIGSQIISRSTVKAMRKNVLAKCYG
GDISRKKKLLEKQKEGKKRMKQIGRVEIPQEAFLAVLQIDNK

Sequences:

>Translated_602_residues
MSSDPMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLEAREMEAQVLDSNPIERERGITIKAQSVSLLYKAQDGQNYHLNL
IDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQSVANCYTAVEQGLEVVPILNKIDLPTADTERAKAEIETVIGIDA
SEAVAVSAKTGLYVEQVLEAIVQRIPAPQPRDTEKLQALIIDSWFDNYLGVVSLVRVMQGEITPGNKLLVMSTGRSHQVD
AVGVFTPKRKTLAKLTAGEVGWVTASIKDVHGAPVGDTLTLTSDPAPKPLPGFQEVQPRVFAGLFPVDAEDYPDLREALE
KLRLNDAALRFEPENSEAMGFGFRCGFLGMLHMEIVQERLEREYDLNLITTAPTVIYEVLKNDGTLVAMDNPAKMPPINQ
INEIREPIIRSNILTPPDYVGAVITLCEEKRGSQISITYLGNQVQVAYELPMAEVVLDFFDKLKSVTRGYASLDYHFLRF
QEGPFVRVDTLINGDRVDALSVIVHRHQAERRGRELCEKMKDLIPRQMFDVAIQAAIGSQIISRSTVKAMRKNVLAKCYG
GDISRKKKLLEKQKEGKKRMKQIGRVEIPQEAFLAVLQIDNK
>Mature_601_residues
SSDPMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLEAREMEAQVLDSNPIERERGITIKAQSVSLLYKAQDGQNYHLNLI
DTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQSVANCYTAVEQGLEVVPILNKIDLPTADTERAKAEIETVIGIDAS
EAVAVSAKTGLYVEQVLEAIVQRIPAPQPRDTEKLQALIIDSWFDNYLGVVSLVRVMQGEITPGNKLLVMSTGRSHQVDA
VGVFTPKRKTLAKLTAGEVGWVTASIKDVHGAPVGDTLTLTSDPAPKPLPGFQEVQPRVFAGLFPVDAEDYPDLREALEK
LRLNDAALRFEPENSEAMGFGFRCGFLGMLHMEIVQERLEREYDLNLITTAPTVIYEVLKNDGTLVAMDNPAKMPPINQI
NEIREPIIRSNILTPPDYVGAVITLCEEKRGSQISITYLGNQVQVAYELPMAEVVLDFFDKLKSVTRGYASLDYHFLRFQ
EGPFVRVDTLINGDRVDALSVIVHRHQAERRGRELCEKMKDLIPRQMFDVAIQAAIGSQIISRSTVKAMRKNVLAKCYGG
DISRKKKLLEKQKEGKKRMKQIGRVEIPQEAFLAVLQIDNK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=607, Percent_Identity=48.9291598023064, Blast_Score=624, Evalue=1e-179,
Organism=Homo sapiens, GI4503483, Length=148, Percent_Identity=42.5675675675676, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.8646616541353, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI25306283, Length=138, Percent_Identity=42.7536231884058, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI25306287, Length=138, Percent_Identity=42.7536231884058, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI19923640, Length=138, Percent_Identity=42.7536231884058, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI18390331, Length=163, Percent_Identity=32.5153374233129, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=45.4545454545455, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=45.4545454545455, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=45.4545454545455, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI217272894, Length=143, Percent_Identity=36.3636363636364, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI217272892, Length=143, Percent_Identity=36.3636363636364, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI34147630, Length=215, Percent_Identity=31.6279069767442, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=599, Percent_Identity=68.9482470784641, Blast_Score=847, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=402, Percent_Identity=29.1044776119403, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789738, Length=162, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=30.1282051282051, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789559, Length=231, Percent_Identity=29.4372294372294, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790412, Length=134, Percent_Identity=32.8358208955224, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789737, Length=134, Percent_Identity=32.8358208955224, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=40.327868852459, Blast_Score=469, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17506493, Length=160, Percent_Identity=38.125, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17556745, Length=159, Percent_Identity=35.8490566037736, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=139, Percent_Identity=36.6906474820144, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=139, Percent_Identity=36.6906474820144, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.1724137931034, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=141, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=142, Percent_Identity=35.9154929577465, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI32566303, Length=245, Percent_Identity=28.9795918367347, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=603, Percent_Identity=45.6053067993366, Blast_Score=538, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6324707, Length=196, Percent_Identity=37.2448979591837, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320593, Length=196, Percent_Identity=37.2448979591837, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323098, Length=214, Percent_Identity=32.7102803738318, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324166, Length=152, Percent_Identity=38.8157894736842, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322359, Length=116, Percent_Identity=37.0689655172414, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=278, Percent_Identity=28.7769784172662, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI78706572, Length=603, Percent_Identity=43.4494195688226, Blast_Score=517, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24585711, Length=152, Percent_Identity=38.1578947368421, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24585713, Length=152, Percent_Identity=38.1578947368421, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24585709, Length=152, Percent_Identity=38.1578947368421, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=43.8848920863309, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24582462, Length=163, Percent_Identity=33.7423312883436, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI221458488, Length=148, Percent_Identity=37.8378378378378, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21357743, Length=141, Percent_Identity=34.7517730496454, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28572034, Length=264, Percent_Identity=28.4090909090909, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921738, Length=282, Percent_Identity=30.1418439716312, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_XYLF2 (B2I601)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830064.1
- ProteinModelPortal:   B2I601
- SMR:   B2I601
- GeneID:   6203752
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1372
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66715; Mature: 66583

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDPMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLEAREMEAQVLDSNPIERERGITIK
CCCCCCHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHCCCEEE
AQSVSLLYKAQDGQNYHLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQSVAN
EEEEEEEEEECCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
CYTAVEQGLEVVPILNKIDLPTADTERAKAEIETVIGIDASEAVAVSAKTGLYVEQVLEA
HHHHHHCCCEEEECHHHCCCCCCCCHHHHHHHHHHEECCCCCEEEEECCCCHHHHHHHHH
IVQRIPAPQPRDTEKLQALIIDSWFDNYLGVVSLVRVMQGEITPGNKLLVMSTGRSHQVD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
AVGVFTPKRKTLAKLTAGEVGWVTASIKDVHGAPVGDTLTLTSDPAPKPLPGFQEVQPRV
EEEEECCCHHHHHHHCCCCCEEEEEEHHHCCCCCCCCEEEECCCCCCCCCCCHHHHCHHH
FAGLFPVDAEDYPDLREALEKLRLNDAALRFEPENSEAMGFGFRCGFLGMLHMEIVQERL
HHCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYDLNLITTAPTVIYEVLKNDGTLVAMDNPAKMPPINQINEIREPIIRSNILTPPDYV
HHHCCCEEEECHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
GAVITLCEEKRGSQISITYLGNQVQVAYELPMAEVVLDFFDKLKSVTRGYASLDYHFLRF
HHHHHHHHHCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHCCEEEEEE
QEGPFVRVDTLINGDRVDALSVIVHRHQAERRGRELCEKMKDLIPRQMFDVAIQAAIGSQ
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IISRSTVKAMRKNVLAKCYGGDISRKKKLLEKQKEGKKRMKQIGRVEIPQEAFLAVLQID
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEC
NK
CC
>Mature Secondary Structure 
SSDPMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLEAREMEAQVLDSNPIERERGITIK
CCCCCHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHCCCEEE
AQSVSLLYKAQDGQNYHLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQSVAN
EEEEEEEEEECCCCCEEEEEEECCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
CYTAVEQGLEVVPILNKIDLPTADTERAKAEIETVIGIDASEAVAVSAKTGLYVEQVLEA
HHHHHHCCCEEEECHHHCCCCCCCCHHHHHHHHHHEECCCCCEEEEECCCCHHHHHHHHH
IVQRIPAPQPRDTEKLQALIIDSWFDNYLGVVSLVRVMQGEITPGNKLLVMSTGRSHQVD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
AVGVFTPKRKTLAKLTAGEVGWVTASIKDVHGAPVGDTLTLTSDPAPKPLPGFQEVQPRV
EEEEECCCHHHHHHHCCCCCEEEEEEHHHCCCCCCCCEEEECCCCCCCCCCCHHHHCHHH
FAGLFPVDAEDYPDLREALEKLRLNDAALRFEPENSEAMGFGFRCGFLGMLHMEIVQERL
HHCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYDLNLITTAPTVIYEVLKNDGTLVAMDNPAKMPPINQINEIREPIIRSNILTPPDYV
HHHCCCEEEECHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
GAVITLCEEKRGSQISITYLGNQVQVAYELPMAEVVLDFFDKLKSVTRGYASLDYHFLRF
HHHHHHHHHCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHCCEEEEEE
QEGPFVRVDTLINGDRVDALSVIVHRHQAERRGRELCEKMKDLIPRQMFDVAIQAAIGSQ
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IISRSTVKAMRKNVLAKCYGGDISRKKKLLEKQKEGKKRMKQIGRVEIPQEAFLAVLQID
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEEC
NK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA