Click here to switch to the map view.

The map label for this gene is gloB

Identifier: 182681834

GI number: 182681834

Start: 1429599

End: 1430375

Strand: Direct

Name: gloB

Synonym: XfasM23_1299

Alternate gene names: 182681834

Gene position: 1429599-1430375 (Clockwise)

Preceding gene: 182681830

Following gene: 182681835

Centisome position: 56.38

GC content: 51.74

Gene sequence:

>777_bases
ATGCGACTGACTCCACTCCCCGCATTCGACAACAATTACATCTGGACATTGATTGCCCCTGACGGCCGAGCAATCATCGT
TGATCCCGGACAAGCCTTGCCAATATTAGAAGCTCATTCCAAAGGACTCATCCCCACTGCAATCCTATTGACACACCACC
ACGCCGATCACATCGGCGGAGTGCCAGAACTGCTAGAACGCTGGCCAACACTTCCCGTCTATGCCCCACACGACACACGC
ATCGCACTGAATTACCACCGCATTGGCGAGGGAGATTCATTGAACATCCTTGGTATGCGATTCCAAGTTATCCACACCCC
AGGGCATACTCACAGCCACTTAACCTTCATAGGTAACGATCTACTGTTCTGCGGTGACACACTATTCAGCCTAGGTTGTG
GGCAGATCTTTGAAGGAACCCCCACACAGATGCTCGCATCATTGCAACGATTGGCTGCACTGCCCATACAAACCCGCGTA
TGCTGCGGTCACGAATACACATTGTCCAACGCTGCATTCGCACTGCACGTCGACCCCACCAACACCGCCTTACAAAAACG
TCGACAACAAGCAAACGCCATGCGTCTCGCAGGCCTCCCCACATTGCCTATTTCACTCGAAAGCGAACTAAACACCAACC
CATTCCTGCGTACGGCTGCACCTACCATCCATGCAGCAACAGCTACTCACCTGCAACGAACACCAATAGATGAAGTAGAA
GTATTCGCTACGCTACGCCACTGGAAAAACAACTTCCCCATAAAAAACATCCCCTGA

Upstream 100 bases:

>100_bases
GGATACTCAAACTGAATTAACAAACGCCCTGCCCCCGTTTCGAACCAGGATGAGAGACCCGTTTGACATGGAGGCAGAGC
GGCAGGCATGGTCACATTCT

Downstream 100 bases:

>100_bases
TGTGCTTGTGCTGCTTGTGCTGCTTGTGCTGCTTGTGCTGCTTGTGCTGCTTGTGCTGCTTGTGCTGCTTGTGCTGTACA
GAATTGAAAAACTTCAGGCC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRLTPLPAFDNNYIWTLIAPDGRAIIVDPGQALPILEAHSKGLIPTAILLTHHHADHIGGVPELLERWPTLPVYAPHDTR
IALNYHRIGEGDSLNILGMRFQVIHTPGHTHSHLTFIGNDLLFCGDTLFSLGCGQIFEGTPTQMLASLQRLAALPIQTRV
CCGHEYTLSNAAFALHVDPTNTALQKRRQQANAMRLAGLPTLPISLESELNTNPFLRTAAPTIHAATATHLQRTPIDEVE
VFATLRHWKNNFPIKNIP

Sequences:

>Translated_258_residues
MRLTPLPAFDNNYIWTLIAPDGRAIIVDPGQALPILEAHSKGLIPTAILLTHHHADHIGGVPELLERWPTLPVYAPHDTR
IALNYHRIGEGDSLNILGMRFQVIHTPGHTHSHLTFIGNDLLFCGDTLFSLGCGQIFEGTPTQMLASLQRLAALPIQTRV
CCGHEYTLSNAAFALHVDPTNTALQKRRQQANAMRLAGLPTLPISLESELNTNPFLRTAAPTIHAATATHLQRTPIDEVE
VFATLRHWKNNFPIKNIP
>Mature_258_residues
MRLTPLPAFDNNYIWTLIAPDGRAIIVDPGQALPILEAHSKGLIPTAILLTHHHADHIGGVPELLERWPTLPVYAPHDTR
IALNYHRIGEGDSLNILGMRFQVIHTPGHTHSHLTFIGNDLLFCGDTLFSLGCGQIFEGTPTQMLASLQRLAALPIQTRV
CCGHEYTLSNAAFALHVDPTNTALQKRRQQANAMRLAGLPTLPISLESELNTNPFLRTAAPTIHAATATHLQRTPIDEVE
VFATLRHWKNNFPIKNIP

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=264, Percent_Identity=36.3636363636364, Blast_Score=154, Evalue=6e-38,
Organism=Homo sapiens, GI94538322, Length=264, Percent_Identity=36.3636363636364, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI14150041, Length=255, Percent_Identity=35.6862745098039, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI116642887, Length=227, Percent_Identity=34.3612334801762, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI21703352, Length=235, Percent_Identity=33.6170212765957, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI46361987, Length=202, Percent_Identity=37.6237623762376, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1786406, Length=254, Percent_Identity=45.2755905511811, Blast_Score=207, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17536925, Length=226, Percent_Identity=34.5132743362832, Blast_Score=123, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6320478, Length=270, Percent_Identity=29.6296296296296, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324614, Length=272, Percent_Identity=28.6764705882353, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356335, Length=266, Percent_Identity=37.9699248120301, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24667711, Length=265, Percent_Identity=38.1132075471698, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24667703, Length=263, Percent_Identity=37.6425855513308, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_XYLF2 (B2I5S5)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829994.1
- ProteinModelPortal:   B2I5S5
- SMR:   B2I5S5
- GeneID:   6202130
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1299
- HOGENOM:   HBG753931
- OMA:   FGHEYTL
- ProtClustDB:   CLSK497122
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28495; Mature: 28495

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTPLPAFDNNYIWTLIAPDGRAIIVDPGQALPILEAHSKGLIPTAILLTHHHADHIGG
CCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCEEECCCCCCCCEEEEEEECCHHHCCC
VPELLERWPTLPVYAPHDTRIALNYHRIGEGDSLNILGMRFQVIHTPGHTHSHLTFIGND
HHHHHHHCCCCCEECCCCCEEEEEEEECCCCCCEEEEEEEEEEEECCCCCCCEEEEECCC
LLFCGDTLFSLGCGQIFEGTPTQMLASLQRLAALPIQTRVCCGHEYTLSNAAFALHVDPT
EEEECHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCCEEEEEECCC
NTALQKRRQQANAMRLAGLPTLPISLESELNTNPFLRTAAPTIHAATATHLQRTPIDEVE
HHHHHHHHHHHHHEEECCCCCCCEEECCCCCCCCEEEECCCEEEHHHHHHHHCCCHHHHH
VFATLRHWKNNFPIKNIP
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRLTPLPAFDNNYIWTLIAPDGRAIIVDPGQALPILEAHSKGLIPTAILLTHHHADHIGG
CCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCEEECCCCCCCCEEEEEEECCHHHCCC
VPELLERWPTLPVYAPHDTRIALNYHRIGEGDSLNILGMRFQVIHTPGHTHSHLTFIGND
HHHHHHHCCCCCEECCCCCEEEEEEEECCCCCCEEEEEEEEEEEECCCCCCCEEEEECCC
LLFCGDTLFSLGCGQIFEGTPTQMLASLQRLAALPIQTRVCCGHEYTLSNAAFALHVDPT
EEEECHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCCEEEEEECCC
NTALQKRRQQANAMRLAGLPTLPISLESELNTNPFLRTAAPTIHAATATHLQRTPIDEVE
HHHHHHHHHHHHHEEECCCCCCCEEECCCCCCCCEEEECCCEEEHHHHHHHHCCCHHHHH
VFATLRHWKNNFPIKNIP
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA