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The map label for this gene is leuA

Identifier: 182681653

GI number: 182681653

Start: 1258957

End: 1260516

Strand: Direct

Name: leuA

Synonym: XfasM23_1111

Alternate gene names: 182681653

Gene position: 1258957-1260516 (Clockwise)

Preceding gene: 182681652

Following gene: 182681658

Centisome position: 49.65

GC content: 53.08

Gene sequence:

>1560_bases
GTGAACACGAGCATATCCAGCCAGCCGCCCTGTATCCGGATTTTTGATACCACCCTGCGGGACGGTGAGCAATCTCCCGG
TTGCAGTATGCCTGCACAACAAAAACTGGTCATGGCACGCGCATTAGATGCATTGGGCGTAGACATTATCGAGACTGGTT
TCCCAGCCAGTTCCCAATCCGACTACGAAGCCATGACGCTGATCGCACGCGAGTTACGTAGACCAACTCTGGCAGTACTG
TCACGTTGTCTGCAAGCCGATATCGAAACTTCAGCACGCGCACTGGAATCAGCAACCAAACCACGCTTGCATGTATTTCT
GTCAACCAGCCCATTACACCGCGAGCACAAGCTACGCATGAGCAAGGAACAGGTGCTGGAGTCAGTGCACAAGCATGTGA
GTCTCAGTCGAACCTTGATTGACGATGTGGAATTCTCGGCCGAGGATGCGACGCGCACAGAGGAGGATTTCCTGATAGAG
GTTACCCGCGTGGCAATCGCCGCTGGTGCAACCACGATCAACCTGCCAGACACCGTAGGGTTCAGCACGCCTGAAGAAAT
CCGCAACATGTTTACGCGCGTCATCGCGAACGTCGAAGGTGCAAACAAAGTGATCTTTAGTGCGCACTGCCACGACGATC
TGGGTTTGGCCGTTGCTAACTCGCTGGCAGCCATTGAGGGCGGTGCACGCCAAATCGAATGTACTATCAATGGCATTGGT
GAGCGCGCTGGTAACTGCGCGCTGGAAGAACTCACTATGGTGTTGAAAGTACGCAACGCGTTTTACAACATTGATACTTC
AATCCACACATCACGTATCGTCTCCACCTCCCAGTTACTGCAACGATTGGTTGGCATGCCCGTGCAACGTAACAAGGCAG
TAGTAGGTGCCAATGCCTTTGCACATGAATCGGGTATCCACCAGCACGGTATGCTGCGCCATCGCGGCACCTACGAAATC
ATGCGTCCACAAGAAGTCGGTTGGGTATGTTCGCATATGGTGCTCGGCCGCCATAGCGGCCGTGCTGCGGTCGAACAGCG
TCTACGCGCACTGGGCTACTTGCTGGAGGAAGAAGATCTAAAACTGGTATTTGAAGAATTCAAACAGCTTTGTGAGAAAC
AGCGTTTGGTCACCGATGTCGACCTGCAAGTACTGATGCAAGATACAACAGTACAGCATGGCTATCGCTTGGCCTCAATG
ACAATCAGTGATGTTGGTAACCGGGCCAACGCACTGGTGGAATTGTCCGATCCCCAAGGTCAGCGTGTTGCCGAAACTGC
GCAAGGCAACGGCCCAGTAGATGCACTGTTCGGAGCGCTGGCTGCAGCAACTGGGGTCAAACTGGAGTTGGACAGCTATC
AGGTACACAGTGTTGGTATCGGGGCCGATGCACGTGGTGAAGCAAACCTCAGTGTACGCCACAACGATACTCAGTACGAA
GGTACAGGGACCAGTAAAGACATTATCGAAGCCAGTGCGCTTGCTTGGCTAGAGGTCGCCAACCGTTTGCTACGTAACCC
CGAAAATATGCAAAATAAACAAAACACAGCACTGGCCTGA

Upstream 100 bases:

>100_bases
AGATCGCCCCAACAATTGCGGCAATTTACCGCTCCCTTTTAACTGATTCCACCCCATATCCACGCCTGCCAATAGCACTG
CTGTAGAGGAGTATTCACTG

Downstream 100 bases:

>100_bases
TTCGATGCATTACACATCTTGATCATAGTTCCTCTCCGAAACATAGAGATTCAGGTACAAACTGAAGCTCTATGGGCTTC
CCTCAGGAATTGAAAAACCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MNTSISSQPPCIRIFDTTLRDGEQSPGCSMPAQQKLVMARALDALGVDIIETGFPASSQSDYEAMTLIARELRRPTLAVL
SRCLQADIETSARALESATKPRLHVFLSTSPLHREHKLRMSKEQVLESVHKHVSLSRTLIDDVEFSAEDATRTEEDFLIE
VTRVAIAAGATTINLPDTVGFSTPEEIRNMFTRVIANVEGANKVIFSAHCHDDLGLAVANSLAAIEGGARQIECTINGIG
ERAGNCALEELTMVLKVRNAFYNIDTSIHTSRIVSTSQLLQRLVGMPVQRNKAVVGANAFAHESGIHQHGMLRHRGTYEI
MRPQEVGWVCSHMVLGRHSGRAAVEQRLRALGYLLEEEDLKLVFEEFKQLCEKQRLVTDVDLQVLMQDTTVQHGYRLASM
TISDVGNRANALVELSDPQGQRVAETAQGNGPVDALFGALAAATGVKLELDSYQVHSVGIGADARGEANLSVRHNDTQYE
GTGTSKDIIEASALAWLEVANRLLRNPENMQNKQNTALA

Sequences:

>Translated_519_residues
MNTSISSQPPCIRIFDTTLRDGEQSPGCSMPAQQKLVMARALDALGVDIIETGFPASSQSDYEAMTLIARELRRPTLAVL
SRCLQADIETSARALESATKPRLHVFLSTSPLHREHKLRMSKEQVLESVHKHVSLSRTLIDDVEFSAEDATRTEEDFLIE
VTRVAIAAGATTINLPDTVGFSTPEEIRNMFTRVIANVEGANKVIFSAHCHDDLGLAVANSLAAIEGGARQIECTINGIG
ERAGNCALEELTMVLKVRNAFYNIDTSIHTSRIVSTSQLLQRLVGMPVQRNKAVVGANAFAHESGIHQHGMLRHRGTYEI
MRPQEVGWVCSHMVLGRHSGRAAVEQRLRALGYLLEEEDLKLVFEEFKQLCEKQRLVTDVDLQVLMQDTTVQHGYRLASM
TISDVGNRANALVELSDPQGQRVAETAQGNGPVDALFGALAAATGVKLELDSYQVHSVGIGADARGEANLSVRHNDTQYE
GTGTSKDIIEASALAWLEVANRLLRNPENMQNKQNTALA
>Mature_519_residues
MNTSISSQPPCIRIFDTTLRDGEQSPGCSMPAQQKLVMARALDALGVDIIETGFPASSQSDYEAMTLIARELRRPTLAVL
SRCLQADIETSARALESATKPRLHVFLSTSPLHREHKLRMSKEQVLESVHKHVSLSRTLIDDVEFSAEDATRTEEDFLIE
VTRVAIAAGATTINLPDTVGFSTPEEIRNMFTRVIANVEGANKVIFSAHCHDDLGLAVANSLAAIEGGARQIECTINGIG
ERAGNCALEELTMVLKVRNAFYNIDTSIHTSRIVSTSQLLQRLVGMPVQRNKAVVGANAFAHESGIHQHGMLRHRGTYEI
MRPQEVGWVCSHMVLGRHSGRAAVEQRLRALGYLLEEEDLKLVFEEFKQLCEKQRLVTDVDLQVLMQDTTVQHGYRLASM
TISDVGNRANALVELSDPQGQRVAETAQGNGPVDALFGALAAATGVKLELDSYQVHSVGIGADARGEANLSVRHNDTQYE
GTGTSKDIIEASALAWLEVANRLLRNPENMQNKQNTALA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=501, Percent_Identity=43.7125748502994, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6320019, Length=371, Percent_Identity=27.2237196765499, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6320071, Length=377, Percent_Identity=26.790450928382, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6324682, Length=547, Percent_Identity=25.9597806215722, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=559, Percent_Identity=24.865831842576, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_XYLF2 (B2I596)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829813.1
- ProteinModelPortal:   B2I596
- SMR:   B2I596
- GeneID:   6203922
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1111
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56793; Mature: 56793

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSISSQPPCIRIFDTTLRDGEQSPGCSMPAQQKLVMARALDALGVDIIETGFPASSQS
CCCCCCCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
DYEAMTLIARELRRPTLAVLSRCLQADIETSARALESATKPRLHVFLSTSPLHREHKLRM
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHC
SKEQVLESVHKHVSLSRTLIDDVEFSAEDATRTEEDFLIEVTRVAIAAGATTINLPDTVG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCC
FSTPEEIRNMFTRVIANVEGANKVIFSAHCHDDLGLAVANSLAAIEGGARQIECTINGIG
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCC
ERAGNCALEELTMVLKVRNAFYNIDTSIHTSRIVSTSQLLQRLVGMPVQRNKAVVGANAF
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHH
AHESGIHQHGMLRHRGTYEIMRPQEVGWVCSHMVLGRHSGRAAVEQRLRALGYLLEEEDL
HHHCCCCCCCCHHCCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHH
KLVFEEFKQLCEKQRLVTDVDLQVLMQDTTVQHGYRLASMTISDVGNRANALVELSDPQG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCEEEEECCCCC
QRVAETAQGNGPVDALFGALAAATGVKLELDSYQVHSVGIGADARGEANLSVRHNDTQYE
CHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCEEEEEEECCCCCCCCCCEEEEECCCCCC
GTGTSKDIIEASALAWLEVANRLLRNPENMQNKQNTALA
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure
MNTSISSQPPCIRIFDTTLRDGEQSPGCSMPAQQKLVMARALDALGVDIIETGFPASSQS
CCCCCCCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
DYEAMTLIARELRRPTLAVLSRCLQADIETSARALESATKPRLHVFLSTSPLHREHKLRM
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHC
SKEQVLESVHKHVSLSRTLIDDVEFSAEDATRTEEDFLIEVTRVAIAAGATTINLPDTVG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCC
FSTPEEIRNMFTRVIANVEGANKVIFSAHCHDDLGLAVANSLAAIEGGARQIECTINGIG
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCC
ERAGNCALEELTMVLKVRNAFYNIDTSIHTSRIVSTSQLLQRLVGMPVQRNKAVVGANAF
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHH
AHESGIHQHGMLRHRGTYEIMRPQEVGWVCSHMVLGRHSGRAAVEQRLRALGYLLEEEDL
HHHCCCCCCCCHHCCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHH
KLVFEEFKQLCEKQRLVTDVDLQVLMQDTTVQHGYRLASMTISDVGNRANALVELSDPQG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCEEEEECCCCC
QRVAETAQGNGPVDALFGALAAATGVKLELDSYQVHSVGIGADARGEANLSVRHNDTQYE
CHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCEEEEEEECCCCCCCCCCEEEEECCCCCC
GTGTSKDIIEASALAWLEVANRLLRNPENMQNKQNTALA
CCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA