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The map label for this gene is purU

Identifier: 182681642

GI number: 182681642

Start: 1245166

End: 1246017

Strand: Direct

Name: purU

Synonym: XfasM23_1100

Alternate gene names: 182681642

Gene position: 1245166-1246017 (Clockwise)

Preceding gene: 182681639

Following gene: 182681643

Centisome position: 49.11

GC content: 48.94

Gene sequence:

>852_bases
ATGCGTCACGACTATATCCTCACTTTATCCTGTCCCGACTGTACTGGCCTTGTGTATCGGATCAGTGGCGAGTTGTTTCG
AGCGGGCTGCAATATTCTTGATGCGCAACAATTTGTTGACAAGGAAAGTCATCAGTTTTTTCTTCGCGTGCATTTTGATG
TGGCTGATAGTAGTTCATTGGCAGAACTGCAAAACCAGATCAGCATACTTGCCAAAGCCTATGCAATGCAGTGGCAATTG
CACGATGCGCGCCGACGTTCCCGCCTGCTGGTAATGGTCAGTAAGCAAGGTCACTGCCTTAACGATTTATTGTTCCGTAT
ACACAGTCGGCAATTGCAAGCAAAAATCGTCACTGTCGTTTCCAACCACAATGAGTTTGCCCCTTTAACGGCCTCATACG
GTGTTCCATTCCAGCACTTACCCGTGAATGCAGACAACCGTACTGAACAAGAGGCTCGGATCATACAAATGGTCGAGCGT
GAACAGATCGACTTGGTGATTTTGGCGCGCTATATGCAAATACTTTCACCTGCGTTGTGTGAGGCACTGCTTGGGCGTGC
GATCAACATTCATCACAGTTTCTTGCCCAGTTTCAAGGGTGCCCAACCTTACCATCAAGCACACGCACGCGGGGTAAAAA
TCATCGGTGCCACTGCACACTATGTCACCCATGATTTGGACGAAGGTCCCATCATCGAACAGGACGTAGCACGGGTTGAT
CATTCAATGACGGCACATGATTTGGTGCGTATAGGCAGTGATATCGAGTCGCTGGTCTTAGCACGTGCAGTGAGTCGCCA
TATCGAACATCGGATCTTGCTTAACGGTCATCGGACAGTGGTGTTTCGCTAG

Upstream 100 bases:

>100_bases
TTTCAGTTCGATTTAATAAGCGCAGCTTAGATGAACTACACATTGCTACCATTAAATAATGACCATCCGATTGCAGTAGA
GTTGCATTAGCACCATAAAA

Downstream 100 bases:

>100_bases
AACTCCTTATGCACTGTGAGCCAGCCGGTTTGGTGGATTGTAGATGGGCCATCAGCGATGCTAAATCGAGTCTTCGGGTC
ACTTGCAGCAAGCACAAATG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRHDYILTLSCPDCTGLVYRISGELFRAGCNILDAQQFVDKESHQFFLRVHFDVADSSSLAELQNQISILAKAYAMQWQL
HDARRRSRLLVMVSKQGHCLNDLLFRIHSRQLQAKIVTVVSNHNEFAPLTASYGVPFQHLPVNADNRTEQEARIIQMVER
EQIDLVILARYMQILSPALCEALLGRAINIHHSFLPSFKGAQPYHQAHARGVKIIGATAHYVTHDLDEGPIIEQDVARVD
HSMTAHDLVRIGSDIESLVLARAVSRHIEHRILLNGHRTVVFR

Sequences:

>Translated_283_residues
MRHDYILTLSCPDCTGLVYRISGELFRAGCNILDAQQFVDKESHQFFLRVHFDVADSSSLAELQNQISILAKAYAMQWQL
HDARRRSRLLVMVSKQGHCLNDLLFRIHSRQLQAKIVTVVSNHNEFAPLTASYGVPFQHLPVNADNRTEQEARIIQMVER
EQIDLVILARYMQILSPALCEALLGRAINIHHSFLPSFKGAQPYHQAHARGVKIIGATAHYVTHDLDEGPIIEQDVARVD
HSMTAHDLVRIGSDIESLVLARAVSRHIEHRILLNGHRTVVFR
>Mature_283_residues
MRHDYILTLSCPDCTGLVYRISGELFRAGCNILDAQQFVDKESHQFFLRVHFDVADSSSLAELQNQISILAKAYAMQWQL
HDARRRSRLLVMVSKQGHCLNDLLFRIHSRQLQAKIVTVVSNHNEFAPLTASYGVPFQHLPVNADNRTEQEARIIQMVER
EQIDLVILARYMQILSPALCEALLGRAINIHHSFLPSFKGAQPYHQAHARGVKIIGATAHYVTHDLDEGPIIEQDVARVD
HSMTAHDLVRIGSDIESLVLARAVSRHIEHRILLNGHRTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=175, Percent_Identity=33.7142857142857, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI209869995, Length=175, Percent_Identity=33.7142857142857, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI209869993, Length=175, Percent_Identity=33.7142857142857, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1787483, Length=279, Percent_Identity=40.1433691756272, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1788846, Length=149, Percent_Identity=30.8724832214765, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=217, Percent_Identity=31.3364055299539, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24582400, Length=157, Percent_Identity=29.9363057324841, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32141; Mature: 32141

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHDYILTLSCPDCTGLVYRISGELFRAGCNILDAQQFVDKESHQFFLRVHFDVADSSSL
CCCCEEEEEECCCCCCEEEEECHHHHHHCCCEEHHHHHHCCCCCEEEEEEEEECCCCHHH
AELQNQISILAKAYAMQWQLHDARRRSRLLVMVSKQGHCLNDLLFRIHSRQLQAKIVTVV
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEE
SNHNEFAPLTASYGVPFQHLPVNADNRTEQEARIIQMVEREQIDLVILARYMQILSPALC
ECCCCCCCEEECCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EALLGRAINIHHSFLPSFKGAQPYHQAHARGVKIIGATAHYVTHDLDEGPIIEQDVARVD
HHHHCCEEEHHHHHCCCCCCCCCHHHHHHCCEEEEEEEHHHHHCCCCCCCCCHHHHHHHH
HSMTAHDLVRIGSDIESLVLARAVSRHIEHRILLNGHRTVVFR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCEEEEEC
>Mature Secondary Structure
MRHDYILTLSCPDCTGLVYRISGELFRAGCNILDAQQFVDKESHQFFLRVHFDVADSSSL
CCCCEEEEEECCCCCCEEEEECHHHHHHCCCEEHHHHHHCCCCCEEEEEEEEECCCCHHH
AELQNQISILAKAYAMQWQLHDARRRSRLLVMVSKQGHCLNDLLFRIHSRQLQAKIVTVV
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEE
SNHNEFAPLTASYGVPFQHLPVNADNRTEQEARIIQMVEREQIDLVILARYMQILSPALC
ECCCCCCCEEECCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EALLGRAINIHHSFLPSFKGAQPYHQAHARGVKIIGATAHYVTHDLDEGPIIEQDVARVD
HHHHCCEEEHHHHHCCCCCCCCCHHHHHHCCEEEEEEEHHHHHCCCCCCCCCHHHHHHHH
HSMTAHDLVRIGSDIESLVLARAVSRHIEHRILLNGHRTVVFR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]