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The map label for this gene is aspS

Identifier: 182681544

GI number: 182681544

Start: 1153166

End: 1154935

Strand: Direct

Name: aspS

Synonym: XfasM23_0999

Alternate gene names: 182681544

Gene position: 1153166-1154935 (Clockwise)

Preceding gene: 182681533

Following gene: 182681550

Centisome position: 45.48

GC content: 51.19

Gene sequence:

>1770_bases
ATGCGTACCCATTTCTGCGGCTTGATTAATGAAACATTGATCGGCCACACCGTTACCCTTGCTGGCTGGACCGATGTGGC
CCGTAACCTGGGTGGAGTTTGCTTTATCGATTTACGTGACCACGAAGGGATCGTACAAATCACGGTAGATTCACGTGCCA
TCGACCAAAACAACTCAGAACTGTTCAGGGTTGCTAGTGGCTTGAGTTATGAGGATGTACTACAAGTTGAAGGCGTTGTG
CACGCCCGTCATGCGGTCAACGATAAGATCAAGACCGGCAAGGTCGAAGTGATTGCTACAAAAATCAAGATACTCAATAA
GGCTGCACCTTTACCGTTCCATGCTCACGAAAACCCAGGCGAAGATATACGTCTGAAATACCGCTACCTGGATCTGCGCC
GCCCGGAAATGCAGCGTATGCAACGTACGCGTATCAAACTGGTACAGGCACTACGTCGCCATCTGGACATGCACGGTTTC
CAAGACATTGAAACGCCAATCCTAACGAAAGCAACCCCTGAAGGAGCCCGTGACTTTCTTGTTCCCGCCAGAATGCACCC
GGGCGAATTTTACGCCTTACCGCAATCACCACAGCTTTTCAAACAAATACTAATGGTGGCTGGTTTCGATCGTTACTATC
AAATCGCACGCTGTTTCCGTGACGAAGCATTGCGTGCGGATCGCCAACTTGAATTCACCCAACTTGATATGGAGTTTGCT
TTTGTAAGCGAACGTGACGTCCAGGACTTTGTTGAAGAGATGATTCGGAGAGTTTTCAAAGAGGTCGCCGGGATCGAGCT
TGATACAACGTTCCCCCGCATGACATGGAAGGAAGCAATGCGCCGTTTTGGCTCTGACAAACCGGATATGCGCATCAATC
TAGAACTGATTGATGTTGCCGCACTCGTTGCTGACAGCACCTTCACCCCATTTACCAATGCCGTTGCGCATCCCAACGGT
CGTGTTGCCGCATTGCGTATCCCCAGAGGCGCAGTATTGTCGCGCAAGCAAATTGATGAATATGCCGCCTACACAGCCAA
GTATGGTGCCACAGGACTTGCTTACGCCAAACTCGCCCCTACAGGCGAAATCACTTCGCCAATCGCTAAATTTTTTTCCG
AGGATGCCTTTGCTGCACTACTCTCACACATCGGAGCGGAGAAAGGCGACATCGTGTTTTTCGGTGCTGGAAACTACAAC
AAAGTCTCCGACTTCATGGGCGCGTTACGCCTGAAAGCCGGCAAAGATTTCGCCCTAATCACCGCAGACTGGCGCCCATT
GTGGGTCACCGACTTTCCAATGTTTGAGTGGGACGAAGAAGCGCAGCGTTACGTTGCCTTACATCACCCCTTCACTGCAC
CAGCAGCCATTAACGATATTGACGAATTACGCACACACGCCAGGACAGCCTTGTCCCGCGGCTATGACATGGTACTCAAT
GGCAACGAAATTGGCGGCGGCTCTATCCGTATTCACCGTCCGGAAATGCAACGTGCAGTCTTTGAACTACTCGGCATTAC
TGAAGATGAAGCACGCGCCAAATTTGGTTTCCTCCTTGACGCACTGAATTATGGTGCCCCACCACACGGAGGGATTGCCT
TTGGCATTGACCGCATCGCCGCACTGATCGCCGGCACTGAATCAATCCGCGATGTTATTCCTTTTCCAAAGACCACCGGC
GCACAATGCCTGATGACCGATGCACCCTCCCCCATCTCGGAAGAGCAACTCTCTGAGATCCATGTGATAACGAAGAAACT
CACGCCATGA

Upstream 100 bases:

>100_bases
GCCCCAGACACCAGCCAGAATTTGCCGAGGTGAAGCGTGAGCCGCAGAATATGCAGCTATCCGACTTCATTCTGAGTCGA
TCGTTCATTGGGGAATTCCG

Downstream 100 bases:

>100_bases
TGCAAAAACAATAGAGGCGATCCGTAATTGCTTGATTCCCCATATCCAACCAATTGCATAACGGCTAAAAGCAGAACATT
GGACCCGGGGACTCCTCCCC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MRTHFCGLINETLIGHTVTLAGWTDVARNLGGVCFIDLRDHEGIVQITVDSRAIDQNNSELFRVASGLSYEDVLQVEGVV
HARHAVNDKIKTGKVEVIATKIKILNKAAPLPFHAHENPGEDIRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDMHGF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVSERDVQDFVEEMIRRVFKEVAGIELDTTFPRMTWKEAMRRFGSDKPDMRINLELIDVAALVADSTFTPFTNAVAHPNG
RVAALRIPRGAVLSRKQIDEYAAYTAKYGATGLAYAKLAPTGEITSPIAKFFSEDAFAALLSHIGAEKGDIVFFGAGNYN
KVSDFMGALRLKAGKDFALITADWRPLWVTDFPMFEWDEEAQRYVALHHPFTAPAAINDIDELRTHARTALSRGYDMVLN
GNEIGGGSIRIHRPEMQRAVFELLGITEDEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALIAGTESIRDVIPFPKTTG
AQCLMTDAPSPISEEQLSEIHVITKKLTP

Sequences:

>Translated_589_residues
MRTHFCGLINETLIGHTVTLAGWTDVARNLGGVCFIDLRDHEGIVQITVDSRAIDQNNSELFRVASGLSYEDVLQVEGVV
HARHAVNDKIKTGKVEVIATKIKILNKAAPLPFHAHENPGEDIRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDMHGF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVSERDVQDFVEEMIRRVFKEVAGIELDTTFPRMTWKEAMRRFGSDKPDMRINLELIDVAALVADSTFTPFTNAVAHPNG
RVAALRIPRGAVLSRKQIDEYAAYTAKYGATGLAYAKLAPTGEITSPIAKFFSEDAFAALLSHIGAEKGDIVFFGAGNYN
KVSDFMGALRLKAGKDFALITADWRPLWVTDFPMFEWDEEAQRYVALHHPFTAPAAINDIDELRTHARTALSRGYDMVLN
GNEIGGGSIRIHRPEMQRAVFELLGITEDEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALIAGTESIRDVIPFPKTTG
AQCLMTDAPSPISEEQLSEIHVITKKLTP
>Mature_589_residues
MRTHFCGLINETLIGHTVTLAGWTDVARNLGGVCFIDLRDHEGIVQITVDSRAIDQNNSELFRVASGLSYEDVLQVEGVV
HARHAVNDKIKTGKVEVIATKIKILNKAAPLPFHAHENPGEDIRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDMHGF
QDIETPILTKATPEGARDFLVPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVSERDVQDFVEEMIRRVFKEVAGIELDTTFPRMTWKEAMRRFGSDKPDMRINLELIDVAALVADSTFTPFTNAVAHPNG
RVAALRIPRGAVLSRKQIDEYAAYTAKYGATGLAYAKLAPTGEITSPIAKFFSEDAFAALLSHIGAEKGDIVFFGAGNYN
KVSDFMGALRLKAGKDFALITADWRPLWVTDFPMFEWDEEAQRYVALHHPFTAPAAINDIDELRTHARTALSRGYDMVLN
GNEIGGGSIRIHRPEMQRAVFELLGITEDEARAKFGFLLDALNYGAPPHGGIAFGIDRIAALIAGTESIRDVIPFPKTTG
AQCLMTDAPSPISEEQLSEIHVITKKLTP

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=605, Percent_Identity=40, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI194272210, Length=253, Percent_Identity=28.0632411067194, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI5031815, Length=253, Percent_Identity=28.0632411067194, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI45439306, Length=255, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1788173, Length=594, Percent_Identity=52.1885521885522, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI1790571, Length=306, Percent_Identity=27.1241830065359, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1789256, Length=325, Percent_Identity=25.8461538461538, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI32566633, Length=593, Percent_Identity=36.5935919055649, Blast_Score=335, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17551876, Length=275, Percent_Identity=26.1818181818182, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994340, Length=266, Percent_Identity=24.4360902255639, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=266, Percent_Identity=24.4360902255639, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=266, Percent_Identity=24.4360902255639, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=640, Percent_Identity=32.8125, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6323011, Length=250, Percent_Identity=30.4, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=258, Percent_Identity=27.5193798449612, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24584738, Length=599, Percent_Identity=37.8964941569282, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640849, Length=258, Percent_Identity=27.5193798449612, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640851, Length=258, Percent_Identity=27.5193798449612, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17136276, Length=241, Percent_Identity=27.8008298755187, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_XYLF2 (B2I4Y5)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829704.1
- ProteinModelPortal:   B2I4Y5
- SMR:   B2I4Y5
- GeneID:   6203589
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0999
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66042; Mature: 66042

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTHFCGLINETLIGHTVTLAGWTDVARNLGGVCFIDLRDHEGIVQITVDSRAIDQNNSE
CCCCHHHHHHHHHHCCEEEECCHHHHHHHCCCEEEEEEECCCCEEEEEECCEECCCCCHH
LFRVASGLSYEDVLQVEGVVHARHAVNDKIKTGKVEVIATKIKILNKAAPLPFHAHENPG
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCC
EDIRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDMHGFQDIETPILTKATPEGARDFL
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHCE
VPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
ECCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECEEEE
FVSERDVQDFVEEMIRRVFKEVAGIELDTTFPRMTWKEAMRRFGSDKPDMRINLELIDVA
ECCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEEEEHHH
ALVADSTFTPFTNAVAHPNGRVAALRIPRGAVLSRKQIDEYAAYTAKYGATGLAYAKLAP
HHHHCCCCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
TGEITSPIAKFFSEDAFAALLSHIGAEKGDIVFFGAGNYNKVSDFMGALRLKAGKDFALI
CCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEE
TADWRPLWVTDFPMFEWDEEAQRYVALHHPFTAPAAINDIDELRTHARTALSRGYDMVLN
EECCCEEEEECCCCCCCCHHHHHEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEE
GNEIGGGSIRIHRPEMQRAVFELLGITEDEARAKFGFLLDALNYGAPPHGGIAFGIDRIA
CCEECCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ALIAGTESIRDVIPFPKTTGAQCLMTDAPSPISEEQLSEIHVITKKLTP
HHHHCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRTHFCGLINETLIGHTVTLAGWTDVARNLGGVCFIDLRDHEGIVQITVDSRAIDQNNSE
CCCCHHHHHHHHHHCCEEEECCHHHHHHHCCCEEEEEEECCCCEEEEEECCEECCCCCHH
LFRVASGLSYEDVLQVEGVVHARHAVNDKIKTGKVEVIATKIKILNKAAPLPFHAHENPG
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCC
EDIRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDMHGFQDIETPILTKATPEGARDFL
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCHHCE
VPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
ECCCCCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECEEEE
FVSERDVQDFVEEMIRRVFKEVAGIELDTTFPRMTWKEAMRRFGSDKPDMRINLELIDVA
ECCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEEEEHHH
ALVADSTFTPFTNAVAHPNGRVAALRIPRGAVLSRKQIDEYAAYTAKYGATGLAYAKLAP
HHHHCCCCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
TGEITSPIAKFFSEDAFAALLSHIGAEKGDIVFFGAGNYNKVSDFMGALRLKAGKDFALI
CCCHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEE
TADWRPLWVTDFPMFEWDEEAQRYVALHHPFTAPAAINDIDELRTHARTALSRGYDMVLN
EECCCEEEEECCCCCCCCHHHHHEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCEEEEE
GNEIGGGSIRIHRPEMQRAVFELLGITEDEARAKFGFLLDALNYGAPPHGGIAFGIDRIA
CCEECCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ALIAGTESIRDVIPFPKTTGAQCLMTDAPSPISEEQLSEIHVITKKLTP
HHHHCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA